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Citations for Millipore Sigma :
7601 - 7650 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... followed by concentration to approximately 200 μl on an Amicon® Ultra-15 Centrifugal Filter with Ultracel-3k membrane (UFC9003, Millipore) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... from Barcelona (Spain) was filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts) to remove bacteria ...
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bioRxiv - Cancer Biology 2023Quote: ... and 25-50 µg per sample were run on 10% homemade SDS-PAGE gels with transfer to PVDF membranes (Millipore #IPFL00010). Membrane blocking was performed with Odyssey PBS blocking buffer (LI-COR #927-40000 ...
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bioRxiv - Molecular Biology 2023Quote: ... #CRL-4031) maintained in serum free media were seeded on the bottom of collagen-coated permeable transwell membranes (EMD Millipore, #PICM01250) and allowed to form epithelial monolayers ...
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bioRxiv - Neuroscience 2023Quote: ... Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques ...
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bioRxiv - Neuroscience 2023Quote: ... solubilized GluA2 or GluA2-γ2 (1 nmol) and membrane scaffold proteins (50 μL, final concentration 1 mg/mL) (MSP1E3D1, Sigma‒ Aldrich) were added ...
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bioRxiv - Biochemistry 2023Quote: ... After washing the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, A2179) in PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... The integrity of the cellular barrier was monitored daily by transepithelial electrical resistance (TEER) across the permeable membrane using a Millicell ESR-2 Voltohmmeter (EMD Millipore) and was confirmed to have increased over 30 days in culture (Supplemental Figure 4A) ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween 20 (Sigma-Aldrich, St. Louis, MO, USA) (TBS-T ...
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bioRxiv - Genomics 2023Quote: ... Samples were separated on SDS-PAGE (stacking 4% and resolving (8% top and 15% bottom)) and the protein-resolved gel was transferred onto a PVDF membrane (Millipore, #IPVH00010) in a 1X transfer buffer (25 mM Tris ...
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bioRxiv - Genetics 2023Quote: ... Equal amounts of protein were separated on a SDS-PAGE gel and transferred to an Immobilon-P polyvinylidene difluoride (PDVF) membrane (Millipore, IPVH00010). Membranes were blocked in PBS with 3% milk powder (Sigma ...
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bioRxiv - Genetics 2023Quote: ... The next morning membranes were washed once with blocking buffer for 5-10min and incubated with horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:10 000 in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cell-free extracts were then resolved by SDS-PAGE using 12.5%-15% polyacrylamide gels and subsequently electro-blotted onto a 0.45 μm polyvinylidene difluoride (PVDF) Immobilon®-P membrane (Merck Millipore, Sigma Aldrich) at 100 V for 90 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were electrophoresed on polyacrylamide gels transferred onto PVDF membranes and probed using the following antibodies: mouse anti-beta-Actin (Sigma #A1978), mouse anti-Vinculin (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (Supplementary Table 1) in 3% dried milk (Marvel) or 3% BSA (Sigma Aldrich) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were washed three times with PBST for 5 minutes each before incubating with anti-mouse secondary antibodies (Sigma-Aldrich, F1804) for at least 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were exposed to either fluorophore-conjugated secondary antibodies (Li-Cor; 1:2000) or HRP-conjugated secondary antibodies (Sigma; 1:3000) in TBST with 5% milk for 30 min (Li-Cor ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the phage suspensions were further filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts), diluted and plated as indicated in the previous paragraph to verify the uniformity of the plaques ...
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bioRxiv - Cell Biology 2023Quote: ... Rinsed membranes were incubated in primary antibody solution (primary antibody reserved in 5% bovine serum albumin, BSA, Sigma, 0.1% (w/v) NaN3 in TBS ...
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 30 μg of protein was separated on a 12% polyacrylamide gel and transferred onto a PVDF membrane (Immobilon-P, Millipore, IPVH00010) in a 20 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μg of protein lysate was electrophoresed on 12% SDS-polyacrylamide gels and transferred to PVDF membranes (EMD Millipore, Burlington, MA). After blocking with 5% milk ...
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bioRxiv - Microbiology 2024Quote: ... the proteins on the membrane were stained with primary antibodies diluted in PBS-T (1x PBS with 0.2% (v/v) Tween 20 (Sigma-Aldrich, #P9416) and 0.1% (v/v ...
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bioRxiv - Immunology 2024Quote: ... Proteins from cell lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (Millipore IPVH00010). For native blots ...
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bioRxiv - Microbiology 2024Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were equilibrated in pre-chilled transfer buffer with 15% methanol and then protein was transferred to a 0.45 µm nitrocellulose membrane (Sigma catalog# 10600002) via wet electroblotting on ice for 1 h at 300 mV ...
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bioRxiv - Biochemistry 2024Quote: ... of the three cell lines were separated in a 12% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a polyvinylidene difluoride membrane (PVDF) (EMD Millipore) at 100V for 1h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein 20 ug were separated by SDS-PAGE and further transferred onto 0.2 um PVDF membrane (Millipore, Cat. No. IPVH00010) pre-soaked with ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... equal amounts of proteins were loaded onto polyacrylamide gels (8-12%) under reducing conditions and transferred to Immobilon-P membranes (EMD Millipore). After blocking with 5% BSA (wt/vol ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were blocked with 5% (w/v) non-fat dry milk in PBS containing 0.5% (v/v) Tween20 (Sigma Aldrich, USA), and primary antibodies were incubated overnight at 4°C with agitation (table 2) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the membrane blocked with 5% skim milk powder dissolved in PBS-T (1x PBS with 0.1% Tween-20 (Sigma-Aldrich #P1379)) for ∼1 h at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Microtiter plates were incubated at 30 °C for the duration of the time course at “high” shake speed setting and linear shaking mode in a 96 well microtiter plate sealed with a Breathe-Easylll transparent membrane (Sigma-Aldrich). Three biological replicates were processed by growthcurver R package and doubling times were found for all strains (Sprouffske and Wagner 2016).
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bioRxiv - Cancer Biology 2024Quote: ... 1×104 cells/cm2 CSCs plated in triplicates on transwell polycarbonate membranes with cell culture inserts (Millipore Sigma, Cat. No. CLS3428) pre-coated with Matrigel (Corning ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the membranes were retained by vacuum filtration to a Whatman GF/C glass microfiber filter (Sigma-Aldrich, St. Louis, MO, USA) and the free radioligand was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... nitrocellulose membranes were incubated at RT for 2 h with anti-GFP (Miltenyi 130-091-833) or anti-Flag (Sigma A8592) antibodies conjugated with horseradish peroxidase in TBS-T at 1:5000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... 12μm-thick cryosections were converted onto RNAse-free polyethylene terephthalate membrane (PET) slides (Zeiss 415190-9051-000) after these had been coated with poly-L-Lysine (Sigma Aldrich).
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated for 2 hours at room temperature with monoclonal anti-β-actin HRP-conjugated mouse antibody (A3854, Sigma-Aldrich). Membranes were developed with Immobilon® Forte Western HRP Substrate (Cat ...
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bioRxiv - Microbiology 2024Quote: ... were electrophoresed on 10% sodium dodecyl sulphate- polyacrylamide gel and the separated proteins were transferred onto the polyvinylidene difluoride membranes (Millipore, USA) The membranes were blocked with Tris-buffered saline with Tween (TBST ...
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bioRxiv - Pathology 2024Quote: ... the supernatants were collected by centrifugation at 4200 × g for 10 min at room temperature and filtered through a 0.22 μm membrane (Millipore, Billerica, MA). The culture filtrates were then added to pre-cooled CMA medium at a final concentration of 10 % (v/v) ...
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bioRxiv - Systems Biology 2024Quote: ... the membranes were treated with HRP-coupled secondary antibodies (Anti-mouse IgG, Sigma-Aldrich A9044; Anti-rabbit IgG, Sigma-Aldrich A0545) for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... were subjected to SDS-PAGE on a 10% polyacrylamide gel and subsequently transferred to Immobilon-P PVDF-membrane (Merck Millipore, IPVH85R). The membrane was blocked with 5% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was blocked with 5% BSA and incubated with primary antibodies against Tuj-1 (dilution 1:10,000, Sigma, Catalog # T8578) and pan-Ago (dilution 1:1200 ...
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bioRxiv - Microbiology 2024Quote: ... The remaining culture was harvested by centrifugation with the supernatant saved after extraction and filtration through a 0.22 μm PES membrane filter unit (Millipore Express, SLGPR33RB). The concentration of hydrogen peroxide in the supernatants was determined using the Fluorimetric Hydrogen Peroxide Assay Kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... swab samples in VTM were centrifuged at 3200 RPM for 15 minutes and the resulting liquid was passed through a 0.22 µm filter (Membrane Solutions, USA) and then the filtrate was treated with a mix of nucleases: Benzonase (Sigma-Aldrich, USA), Turbo DNAse ...
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bioRxiv - Molecular Biology 2024Quote: ... depending on the molecular weight of the target protein and transferred to 0.45 µm Immobilon®-FL PVDF Membrane (Millipore, IPFL00010). The membranes were blocked with blocking solution (5% w/v milk in Tris-buffered saline ...
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bioRxiv - Microbiology 2024Quote: ... Purification and concentration of □ ERS-1 was carried out with Amicon® Ultra-15 centrifugal filter with Ultracel100 kDa membrane (Merck Millipore) (Sun et al. ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with 3 % BSA for 20 min and incubated with 4 µg anti-FLAG monoclonal antibody (Sigma SAB4200119) for 20 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Hydrogel formation and follicle encapsulation was performed as previously described with the addition of staining follicles with the cell membrane marker PKH26 (2 μM final concentration; Sigma-Aldrich) prior to encapsulation to improve visualization ...
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bioRxiv - Neuroscience 2024Quote: ... 400 µm slices were cut with a vibratome and transferred to a membrane culture insert (Sigma-Aldrich, PICMORG50, St. Gallen, Switzerland). The culture media consisted of 50% MEM (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The basement membrane overlying body wall muscles was enzymatically removed by 0.5 mg/ml collagenase for 10 s (C5138, Sigma-Aldrich, Germany). Integrity of BWMs and nerve cord was visually examined via DIC microscopy ...