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Citations for Millipore Sigma :
7501 - 7550 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Telomere vesicles were extracted from APCs live-labelled with Cy3 TelC telomere probes and the membrane lipid dye PKH67 (Sigma Aldrich). After labelling ...
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bioRxiv - Microbiology 2021Quote: ... DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore, Cork, Ireland) and DNA using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... The membrane was initially blocked with 5% skin milk with PBS buffer for 2 hours and after few PBS rinse the membrane was incubated with monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse (Sigma-Aldrich).
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight at 4°C with a 1:5000 dilution of monoclonal rabbit anti-HA antibodies (Sigma-Aldrich) or 1:5000 dilution of monoclonal rabbit anti-FLAG antibodies (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... Tissue was cut into 410 µM thick sections on a tissue chopper and cultured at the medium/air interface on membranes (Millipore PICMORG50) at 37° C in 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... whole cell lysates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a 0.2μm pore-size nitrocellulose membrane (Millipore-Sigma, Burlington, MA). After transfer ...
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bioRxiv - Immunology 2022Quote: ... 10-15 µg of proteins were loaded onto a 96-well plate with an activated and primed polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma). Proteins adsorbed to the membrane were trypsinized by incubation with the protease for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Purified chromatin was separated by SDS-PAGE and transferred to 0.45 μm polyvinylidene difluoride membranes (Immobilon, Millipore, Merck KGaA, Darmstadt, Germany). Blots were incubated with primary antibodies HDAC1 (Cell Signalling # D5C6U ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were fractionated by 10-15% SDS gels and electro-transferred to polyvinylidene difluoride transfer membranes (#IPVH00010, Millipore, Billerica, MA, USA). The membrane was incubated overnight with primary antibodies and then with secondary antibodies conjugated with horseradish peroxidase (#7074S and #7076S ...
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bioRxiv - Cancer Biology 2022Quote: ... 40-100 μg of the total protein were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto polyvinylidene difluoride (PVDF) membrane (Millipore, Germany) by the wet transfer method ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was immobilized on a nitrocellulose membrane and then probed with antibodies against (6-4)PPs (Cosmo Bio NM-DND-002) or ssDNA (Millipore MAB3034). Chemiluminescence was detected as for protein immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked using 5% nonfat milk and probed with 1:10000 dilution of anti-HA (Sigma-Aldrich, H6908, Lot # 098M4812V).
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: Native PAGE-separated proteins or SDS PAGE-separated proteins were then transferred to PVDF membranes (EMD Millipore, catalog #IPVH00010, lot #R1EB02212) and subjected to Western blotting analysis as described previously (Li et al ...
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bioRxiv - Immunology 2022Quote: ... These samples were collected and concentrated using an Amicon Ultra-15 Centrifugal Filter with Ultracel-10 Membrane (Millipore Sigma, cat. # UFC901024) and buffer exchanged to DBPS with Zeba™ Spin Desalting Columns (7K MWCO ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... 20 µl protein extract were separated on a 12% SDS gel and transferred to Nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO). Anti-S6K1 (phospho T449 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed and probed with either goat anti-mouse horseradish peroxidase or goat anti-rabbit horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10,000 for 1 h (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... Samples were electrophoretic separated on SDS-PAGE gels of sodium dodecyl sulfate-polyacrylamide and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA) at 320 mA for 60 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... The anti-evaporation layer was primed with water and the formed membranes were washed with UltraPure water for 20 hours and subsequently passivated with bovine serum albumin (1%, Sigma-Aldrich) in (50 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were loaded into a 12% SDS-PAGE gels and transferred onto polyvinylidene difluoride membranes (pore size: 0.22 mm, Millipore, Billerica, USA). The membranes were blocked with 5% non-fat milk for 1 h and incubated with primary antibodies against β-actin (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins from cell lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (Millipore IPVH00010). For native blots ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon-FL polyvinylidene difluoride membranes (Millipore, IPFL00010). The membrane was blocked in blocking buffer (Trisbuffered saline with 0.1% Tween 20 (TBST ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was electrophoresed at 170 V for 50 min and transferred at 20 V for 2 hours to PVDF membranes (EMD Millipore). Membranes were blocked with 5% non-fat milk at room temperature for 1 hour and incubated with primary antibodies (see reagents) ...
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bioRxiv - Neuroscience 2022Quote: ... and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET), labeling mixture that was incubated for 10 min at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... 600 μg protein equivalents of exosomes pelleted by ultracentrifugation were resuspended in 500 μl Diluent-C for membrane labeling (Sigma CGLDIL) and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET) ...
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bioRxiv - Molecular Biology 2022Quote: ... TAP- and HTP-tagged proteins were detected after a unique incubation of the membranes with Peroxidase Anti-Peroxidase Complex produced in rabbit (1:2,000; Sigma, Cat# P1291). Chemiluminescence signals were revealed on an Amersham™ Imager 600 (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples dissolved in Laemmli buffer were separated on SDS-PAGE gel and transferred to Immobilon-P PVDF membrane (Millipore, IPVH00010). Transferred protein samples were detected using following primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was washed and subjected to Anti-DIG-AP antibody by using DIG Wash and Block Buffer Set (Sigma, #11585762001) and immonological detection of the membrane was conducted by CDP-Star chemiluminescent substrate included in the northern blotting kit.
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bioRxiv - Neuroscience 2022Quote: ... and afterwards transferred to a methanol- activated Immobilon-P PVDF (poly-vinylidene difluoride) membrane (catalog#IPCVH00010, Merck Millipore Ltd., Carrigtwohill, Ireland) by tank blotting at 250mA for 90 min in a cold room (4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Secreted CPSs from each cryptococcal strain cells were collected and purified by ultrafiltration using an Amicon® system with a membrane cutoff of 10 kDa (Millipore). The effective diameter and the polydispersity of the secreted polysaccharide preparations were measured in a 1 mg/mL solution (weight per volume ...
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bioRxiv - Microbiology 2022Quote: ... Aerobic assays were incubated in a 28°C shaker at 225 rpm in 96-well plates sealed with Breathe-Easy membrane (Sigma-Aldrich). All other O2 conditions were generated in a gas-controlled chamber (Ruskinn Invivo2400 ...
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bioRxiv - Molecular Biology 2023Quote: ... were separated in a 12% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a polyvinylidene difluoride membrane (PVDF) (EMD Millipore) at 100V for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Developmental Biology 2024Quote: ... the lysates were separated on 8% v/v or 12% v/v polyacrylamide gels and transferred to a Polyvinylidene difluoride (PVDF) membrane (Millipore ISEQ00010). Membranes were incubated in 5% w/v milk buffer for 1 h at room temperature and incubated in primary antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoreses (SDS-PAGE) on 15% homemade gel and transferred to Immobilon-P transfer membranes (Millipore Corp).
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bioRxiv - Neuroscience 2024Quote: ... the hippocampus and entorhinal cortex were dissected from the slices and positioned on a fluoro-carbon polymer porous membrane (Millipore, PICM0RG50) with 1 ml of slice culture medium beneath ...
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bioRxiv - Microbiology 2024Quote: ... for 10 minutes in the dark and then filtered through Nuclepore black polycarbonate membrane filters (0.22 µm pore size, Merck Millipore, Darmstadt, Germany) with negative pressure (≤ 20 kPa) ...
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bioRxiv - Neuroscience 2023Quote: ... Membrane was washed 3x the next day with TBST and incubated with anti-mouse HRP (#NXA931V; Sigma-Aldrich, St. Louis, Missouri) at 1:5000 dilution for 1 hour at RT and washed ...
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bioRxiv - Microbiology 2024Quote: ... Greiner Bio-One 96-well U-bottom microtiter plates were inoculated with bacterial strains at an initial OD600 of 0.02 in a final well medium volume of 150 μL and sealed with a Breathe-Easy® sealing membrane (Sigma Aldrich). Inoculated plates were incubated at 37°C with 300 RPM shaking ...
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bioRxiv - Cell Biology 2023Quote: ... CAD cells were plated onto coverslips that had been coated with 10 μg/mL laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... a volume of 20 mL with an OD720 of 0.6 was withdrawn from the culture grown in the desired conditions and vacuum filtered through a Durapore® 0.45 µm PVDF membrane (Merck Millipore-Ireland). The filter with the biomass was immediately inverted into a petri dish with 1.6 ml of pre-cooled methanol for quenching and incubated at −80°C for an hour with periodic rubbing of the filter against the container to aid cell lysis ...
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bioRxiv - Immunology 2024Quote: ... was added to a well of a 24 well plate and a 6.5 mm Transwell® Polycarbonate Membrane insert with a 5.0 µm pore (Costar #3421/Sigma # CLS3421-48EA) was added to each well ...
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bioRxiv - Biochemistry 2023Quote: ... If additional primary antibody incubations were required the membrane was stripped using ReBlot Plus Strong Antibody Stripping Solution (EMD Millipore, 2504), washed and blocked again before being reincubated with primary antibody.
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bioRxiv - Biophysics 2024Quote: ... Viral supernatants were filtered using a 33 mm diameter sterile syringe filter with a 0.45 μm pore size hydrophilic PVDF membrane (Millipore Sigma, #SLHVR33RS) and added to HEK293T cells ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred by wet blot transfer on 0.2 µM pore size PVDF membrane (Amersham, GE10600021) and immunoblotted using MCU (Sigma-Aldrich, HPA016480) according to the standard protocol.
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and/or eGFP fluorescence (Ex/Em: 488 nm / 510 nm) was measured in clear 96 well plates sealed with a Breathe-Easy® membrane (Sigma) with linear shaking at 37°C for 48 h using a Spark 10M plate reader (Tecan).
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bioRxiv - Systems Biology 2023Quote: ... set to “high” shake speed and linear shaking mode in a 24 well microtiter dish plate sealed with a Breathe-EasyⓇ transparent membrane (Sigma-Aldrich). For the data in Supplementary Figure 9 ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...