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Citations for Millipore Sigma :
7451 - 7500 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... in a pre-cooled MLA-80 rotor at 2°C for 30 minutes and then filtered through a 0.45-μm Milliex-HV polyvinylidene fluoride membrane (Millipore, Bedford, MA). The filtrate was loaded onto an equilibrated TOG-column ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fractions were concentrated (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane, EMD Millipore, Merck, Burlington MA, USA) and purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE LS ...
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bioRxiv - Microbiology 2022Quote: ... Approximately equal amounts of purified histones and parasite histones were separated by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Millipore, USA). Chemiluminescent detection of bands was carried out by using Super Signal West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then separated by 10% SDS-PAGE in a Bio-Rad mini-gel system (7×8.4cm) and transferred onto polyvinylidene fluoride membrane (EMD Millipore, IPFL00010). The membrane was blocked with TBS-T (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Tissue lysates were separated by SDS-PAGE gels (4%-12%) and transferred to polyvinylidene difluoride fluorescence membranes (Millipore, Billerica, MA, USA). Non-fat dry milk (NFDM-10% ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein (40 μg) were resolved by SDS-PAGE and transferred to a PVDF membrane (#IPVH00010, Millipore, MA, USA). Membranes were blocked with 1×TBST containing 5% dry milk for 2-hr at room temperature and probed with the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were plated on polyester membrane inserts (0.4 μm pore) in 6 well culture plates (Corning Costar CLS3450-24EA, Sigma-Aldrich, UK), 2-3 slices per insert ...
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bioRxiv - Immunology 2022Quote: ... Antibody preparations for in vivo infusions were micro-filtered (Ultrafree®-CL devices - 0.1 µm PVDF membrane, Merck-Millipore, Darmstadt, Germany), and checked for endotoxins levels using the ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (GenScript).
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bioRxiv - Cell Biology 2022Quote: ... Telomere fragments were detected by a prehybridization step with DIG Easy Hyb Granules for 1h and by the actual hybridization of the membrane with a solution containing Telomere Probe dissolved 1:5000 in DIG Easy Hyb granules (Sigma Aldrich) at 42 °C overnight ...
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bioRxiv - Microbiology 2022Quote: We filtered 14L of seawater for DNA samples and 7L of seawater for RNA samples sequentially through a 3 μm pore size polycarbonate track etch membrane filter (AMD manufacturing, ON, Canada) and a 0.22 μm pore size Sterivex filter (Millipore, MA, USA). Filters were stored in RNALater (ThermoFischer ...
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bioRxiv - Cell Biology 2022Quote: ... The total volume was concentrated to ∼2 ml by spin filtration using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-100 membrane (Sigma-Aldrich). The non-vesicular protein fraction volumes were each collected in 2 ml of PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... in a semi-dry blotting chamber for 45 min at 20 V and 4 °C and crosslinked to the membrane with EDC crosslinking solution (127 mM 1-methylimidazole (Sigma-Aldrich), 163 mM N-(3-dimethylaminopropyl)-N&-ethylcarbodiimide hydrochloride (Sigma-Aldrich) ...
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bioRxiv - Genetics 2020Quote: ... 15 μ l samples were loaded on a 4-20% SDS-PAGE and blotted onto Immobilon P PVDF membrane (Millipore, IPVH00010). Next blocking with 0.5% blocking reagent (Roche ...
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bioRxiv - Immunology 2020Quote: ... T and B cells were incubated either as mixed or separated with 0.4 μm pore sized polyester Corning Transwell® membrane insert (Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of protein per well were run on 10% SDS-polyacrylamide (sodium dodecyl sulphate polyacrylamide) gel electrophoresis and then transferred to PVDF membranes (Millipore, USA). Blots were blocked in 5% bovine serum albumin at room temperature for 1 hour and incubated with primary antibodies overnight at 4° followed by incubation with corresponding secondary antibodies at room temperature for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Membranes were then thoroughly washed (6 × 10 min) with PBS-T before revealed with SigmaFast NBT/BCIP substrate solution (Sigma-Aldrich).
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with the secondary antibody (anti-Mouse-HRP IgG, 1:10,000, Sigma-Aldrich, Saint Louis, MO, USA) diluted in TBS-T for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... 15μg protein was separated by electrophoresis on a gradient (8-20%) SDS-polyacrylamide gel and transferred for 1 hour at 100V onto a polyvinylidene difluoride membrane (Millipore Sigma, catalog number IPVH00010 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on 10% SDS-PAGE gels and electrotransferred onto Immobilon-polyvinylidene difluoride membranes (Millipore Sigma, Burlington, MA). After blocking with 5% non-fat milk ...
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bioRxiv - Immunology 2020Quote: ... 10-15 μg of proteins were loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Biochemistry 2020Quote: The sample was concentrated using Amicon Ultra Spin Concentrators with a 10 kDa molecular weight cut off membrane (Millipore, MA, USA), discarding the permeate ...
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bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
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bioRxiv - Systems Biology 2021Quote: ... the gel was scanned for total protein quantification and the proteins were subsequently transferred to an Immobilon-FL PVDF membrane (CAT # IPF00010, EMD Millipore). The membrane was probed with Rabbit anti-RAN (CAT # PA 1-5783 ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of proteins (40 mg) were first loaded on SDS-PAGE gels for electrophoresis and then transferred onto polyvinylidene difluoride (PVDF) membranes (IPFL00010, Millipore, US) for primary antibody incubation ...
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bioRxiv - Plant Biology 2021Quote: ... The PVDF membrane was then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (diluted 1:10,000; Sigma-Aldrich, Stockholm, Sweden) for 1 h at room temperature followed by three washes with TBST and detection using a Agrisera ECL kit (catalogue no ...
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bioRxiv - Biochemistry 2020Quote: ... The CFS was concentrated to 70 mL through a 10 kDa polyethersulfone membrane discs (Generon, UK) using an Amicon UF cell (Model 8200, Millipore, USA). The concentrated CFS was subjected to n-butanol extraction according to Abo-Amer [41] with a few modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 8 and the α-NOCT antibody was antigen affinity purified with NOCT (64-431) immobilized on Immobilon-P PVDF membrane (Millipore). Guinea pig α-NOCT was eluted from the membrane with 0.1 M glycine and 20% glycerol ...
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bioRxiv - Genetics 2020Quote: ... The membrane was pre hybridized for 5 h at 55 °C in hybridization solution (PerfectHyb™ Plus Hybridization Buffer, Sigma Aldrich). Hybridization of the respective radio-labeled probe was carried out overnight at 55 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Equal volumes from each sample were run on 4-12% Bis-Tris gel (Novex, #NP0322BOX) and transferred onto PVDF membrane (Millipore, #IPFL00010). The membranes were then blocked with Casein-containing buffer (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were then chemically cross-linked to the membrane during 90 min at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma Aldrich). Membranes were prehybridized for 30 min in PerfectHyb™ plus (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal protein amounts (15-25μg) were separated on hand cast 8-20% acrylamide Tris-glycine gels then transferred to Immobilon-P® PVDF membranes (Millipore). Membranes were stained with amido black and rinsed in ddH2O for 5 minutes followed by blocking for 1h on rocker in 5% non-fat dry milk in TBST (Tris-buffered saline ...
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bioRxiv - Genetics 2020Quote: ... Protein samples were resolved on a Bolt™ 10% Bis-Tris Plus gel and transferred onto a polyvinylidene difluoride membrane (Immobilon-P; Millipore). Membranes were probed with anti-H4 (ab10158 ...
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bioRxiv - Immunology 2021Quote: ... The Dil-labelled nanocarriers were visualised using the lipophilic dye excitation wavelength of 514 nm while plasma membranes were labelled with FITC-conjugated cholera toxin (Sigma, C1655) and visualised at the excitation wavelength of 488 nm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Aliquots (40 μg) of protein samples were subjected to 10% SDS-PAGE and transferred to PVDF membranes (Millipore, Bedford, MA, USA). After blocking with 5% nonfat milk (dissolved in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to Immobilon 0.20 μm (LC3, GAPDH) or 0.45 μm (CD9, CD63) pore-sized PVDF membranes (Merck Millipore Ltd., Cork, Ireland) by wet transfer ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was collected and filtered through a 250 ml sterile 0.22μm PES membrane filter (EMD Millipore Corporation, Billerica, MA, USA) to remove any remaining bacterial cells ...
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bioRxiv - Systems Biology 2021Quote: ... The membrane slices were then incubated in their respective primary antibody diluted in 2.5% Bovine Serum Albumin (BSA [Sigma Aldrich, 12660]) in TBS-T solution for overnight with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins extracted from the brain tissues were separated by SDS-PAGE and electro-transferred to a PVDF membrane (Millipore, Bedford, MA). The membrane was blocked with 2% (v/v ...
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bioRxiv - Plant Biology 2022Quote: ... 250 ml log-phase culture cells were removed by centrifugation and the supernatant filtered through 0.22 µm membranes (Millipore, Billerica, MA). Solid phase extractions of the supernatants were done using benzene sulphonic acid cation exchanger on silica (Macherey Nagel ...
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bioRxiv - Microbiology 2020Quote: ... then 5×106 PFUs were resolved on an 8-12% SDS-PAGE gel and transferred to an Immobilon-FL polyvinylidene difluoride membrane (Millipore, IPEL00010). Membranes were probed for 30 min at room temperature or 4°C overnight with antibodies as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were blocked in blocking buffer (5% skim milk, 0.1% Tween in PBS) and incubated with β-Actin (#A5316, Sigma-Aldrich), MYC (#9402 ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were subsequently incubated with the indicated primary antibodies followed by the appropriate horseradish peroxidase-labelled antibodies (1/10000, Sigma-Aldrich or GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Fifty micrograms of protein was fractionated via 10% SDS-PAGE and transferred to Immobilon Transfer Membranes (Millipore Corporation, Billerica, MA, USA). Primary antibodies used in this study included those against β-actin (GTX109639 ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml of conditioned medium were dialysed with dialysis tubing cellulose membranes (Sigma-Aldrich, cut-off 12 kDa, REF: D9777-100FT) against 50 ml DTM supplemented with 15 % FBS at 4 °C in two rounds (16 hours ...
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bioRxiv - Microbiology 2020Quote: ... The water samples were filtered through a 0.2 μm pore size polycarbonate disc filter (Whatman® Nuclepore™ Track-Etched Polycarbonate Membrane, Sigma) to collect microbial biomass ...
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bioRxiv - Microbiology 2020Quote: ... All buffers were prepared using water purified to a resistance of 18.2 MΩ cm-1 and filtered through a 0.2 μm membrane (Millipore, Billerica, MA, USA) before use.
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bioRxiv - Immunology 2021Quote: ... an amount of 15 μg of proteins was loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Microbiology 2020Quote: ... The heated samples were subjected to 12% (v/v) SDS-PAGE and then electrophoretically transferred onto a nitrocellulose membrane (320mA for 2h) (Sigma-Aldrich) in transfer buffer [25mM Tris–HCl pH 7.5 ...