Labshake search
Citations for Millipore Sigma :
7551 - 7600 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... two liters of water were collected in triplicate from surface and bottom waters and concentrated on to a 0.22 μm filter (MF-Millipore Membrane filter) using a portable peristaltic pump at low pressure to minimize cell breakage ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Neuroscience 2023Quote: ... Western Blotting experiments on viral particles were carried out on the collected culture media concentrated with Vivaspin 6 MWCO 30 kDa membranes (Sigma Aldrich). Samples were then reduced and denatured by boiling in 4X Laemmli sample buffer at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The water samples were transferred to the lab in a cooling box where upon arrival 30 ml water from the turbid soda pans and 50 ml from the brown pan were filtered onto 0.1 µm pore size filters (MF-Millipore membrane filter) to collect planktonic microbial cells ...
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bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% bovine serum albumin and probed with the following primary antibodies: β-actin (Sigma, Cat. No. A1978), EGFR (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein per lane (20–50 μg) were loaded onto 4%–12% sodium dodecyl sulfate–polyacrylamide gels (Absin, China) and transferred to polyvinylidene difluoride membranes (Millipore, USA), and the membranes were incubated in 5% skim milk powder (weight/volume ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... After nuclear membrane permeabilization with a 10-min exposure with 0.2% Triton X-100 (Sigma-Aldrich®, St. Louis, MO, USA) solution ...
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bioRxiv - Developmental Biology 2022Quote: ... Fiji was used to improve the cell membrane fluorescence signal with background subtraction (Rolling ball radius 50 pixels) and by applying a 3D gaussian blur filter (Sigma 1.5). 3D segmentation was performed on samples stained with Ecadherin and phalloidin to segment cell membranes ...
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bioRxiv - Immunology 2022Quote: The nuclear protein extracts from the TNF-stimulated A549 cells were subjected to SDS-PAGE and transferred to a nitrocellulose membrane (Millipore, USA) using the transfer buffer (192 mM glycine ...
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bioRxiv - Microbiology 2022Quote: ... Three microliters of cell suspension (whole cell) or cell lysate were spotted on an Immobilon-PSQ transfer membrane (Merck Millipore Ltd.) and air dried ...
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bioRxiv - Cell Biology 2023Quote: ... The gels were transferred at 16V to 0.45µm pore size polyvinylidene difluoride (PVDF) membranes (EMD Millipore Immobilon-FL; catalog no. IPFL85R), or 0.2µm pore size PVDF membranes (EMD Millipore Immobilon-PSQ ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich (A2179)) in PBST ...
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bioRxiv - Cell Biology 2023Quote: ... bilateral hippocampi were collected and digested with 0.22 μm PES membrane-filtered custom digestion solution containing 5 mg/mL deoxyribonuclease I (Sigma, D4527/10KU), 1.5 mM CaCl2 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were subsequently blocked for 1 hour at room temperature in blocking buffer (PBS with 5% skimmed milk powder (Sigma-Aldrich) and 0.1% Tween) ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved in SDS-polyacrylamide gels (4 % stacking and 10 % running) and transferred onto polyvinylidene difluoride (PVDF) membranes (Immobilon-P, EMD Millipore), according to standard protocols ...
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bioRxiv - Physiology 2023Quote: ... The pelleted cells of the cardiac myocyte fraction were resuspended in ice-cold 7 mL Perfusion Buffer and applied onto a 20-µm nylon net membrane (Millipore, SCNY00020). Trapped cells on the membrane were collected with 7 mL Perfusion Buffer ...
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bioRxiv - Plant Biology 2023Quote: ... Forty µg of total protein from each sample was resolved in 12% SDS gel and blotted onto a PVDF membrane (Millipore, USA). The blot was blocked with 5% non-fat milk in TBST buffer (50 mM Tris–HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was quantified by Bradford assay54 and 20 μg of protein of each cell line were separated by SDS-PAGE and electrotransferred to PVDF membranes (Millipore Sigma). The primary antibodies anti-ZIP11 and anti-GAPDH as a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were incubated overnight at 4°C with gentle shaking with rat anti-DAT (1:1000, Millipore Cat# MAB369, RRID:AB_2190413), rabbit anti-TH (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... the 96-well collection plate was submitted to the Molecular Biology Core Facilities at Dana-Farber Cancer Institute where the collection plate was dried down using a permeable membrane (Sigma, A9924) and resuspended in 10 µl of molecular biology grade nuclease-free water ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and the trophozoites were resuspended in 500 μl NaPBS/0.05 % saponin (or permeabilization of cell membranes (Sigma-Aldrich, Merck, Taufkirchen, Germany) and incubated for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride membranes (PVDF, Millipore, USA). The membranes were blocked with 5% low-fat milk for 2 h at room temperature (RT) ...
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bioRxiv - Developmental Biology 2023Quote: ... All proteins were separated in 5-22% gradient SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (PVDF, Millipore, Billerica, MA) using a semi-dry transfer apparatus (Hoeffer) ...
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bioRxiv - Neuroscience 2023Quote: ... Fifteen micrograms of total protein were resolved by denaturing in 10% and 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were then transferred overnight to polyvinylidene difluoride (PVDF) membranes (Cat. # IPFL00010, Millipore Sigma). 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... pestis KIM6+ outer membranes were examined by quantifying the β-lactamase activity as described previously (7) using the chromogenic substrate CENTA (Sigma). Briefly ...
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bioRxiv - Immunology 2023Quote: ... each sample with equal amounts of protein was subjected to SDS–PAGE and transferred to a PVDF membrane (Millipore, Burlington, MA). The membranes were incubated with the primary antibody at 4 ℃ overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of denatured protein homogenate were resolved by SDS-PAGE on 10% polyacrylamide gels and transferred to a polyvinylidene difluoride membrane (Millipore, USA). After blocking at room temperature for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µg of cell extract was loaded into SDS PAGE gel (15% w/v) and transferred to PVDF membrane (Millipore Corp.). After transferring proteins to PVDF ...
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bioRxiv - Microbiology 2023Quote: Water was sampled by filtering 250 ml of water over a Millipore® Isopore polycarbonate membrane filter (0.22-μm pore size; Millipore®) using a vacuum filtration system ...
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bioRxiv - Cell Biology 2022Quote: ... Reactions were stopped by Laemmli buffer addition and samples were subjected to SDS-PAGE and transferred onto polyvinylidene fluoride membranes (Immobilon-FL, EMD Millipore). The incorporated [32P]-ADP-ribose was detected by autoradiography.
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bioRxiv - Cancer Biology 2023Quote: ... An equal amount of protein (10μg) per sample was separated on 10% SDS polyacrylamide gels and then transferred onto PVDF membranes (Millipore, MA, USA). The membrane was blocked with 4% non-fat milk in TBST buffer at room temperature for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated using a 10% sodium dodecyl sulfate polyacrylamide gel and transferred to immobilon polyvinylidene difluoride membranes (EMD Millipore). After blocking for one hour at room temperature in odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P, Millipore, IPVH00010). After blocking the membranes in 5% BSA in TBST (1× TBS (Cell Signalling) ...
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bioRxiv - Immunology 2023Quote: ... the gel was rinsed again with water and soaked in 10X SSC for 30-60 min before capillary transfer to an ImmobilonP membrane (Millipore Sigma), as described previously120 ...
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bioRxiv - Neuroscience 2023Quote: ... The protein extracts were loaded in equal amounts into the wells of a 10% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (EMD Millipore). The blots were incubated overnight with primary antibodies at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the homogenized hippocampi were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then electroblotted onto the polyvinylidene difluoride (PVDF) membranes (EMD Millipore). Blotted membranes were blocked with 5% skim milk in Tris-buffered saline supplemented with 0.1% Tween-20 (TBST ...
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bioRxiv - Bioengineering 2023Quote: ... by dialysis using an Amicon® Ultra-15 Centrifugal Filter Unit with 100 kDa molecular weight cutoff regenerated cellulose membrane (Millipore) or via tangential flow filtration (Repligen ...
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bioRxiv - Biochemistry 2023Quote: ... medium containing viral particles was harvested and filtered using 0.45µm membranes and immediately used to culture H460 cells in the presence of 4 µg/mL polybrene (Sigma, TR-1003-G). After 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Immobilion-P, Millipore, Schwalbach, Germany) by semi-dry blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were separated on 10% freshly cast SDS-PAGE gels followed by a wet-transfer method using a 0.45 μm Immobilon-P PVDF membrane (Millipore, Cat. # IPVH00010). The transfer was performed in the prechilled 1x Tris-glycine transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... 100 μL of the diluted cultures were transferred to a 96-well plate and sealed with a Breathe-Easy sealing membrane (Z380059, Sigma Aldrich). Growth at 37°C ...
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bioRxiv - Genetics 2023Quote: ... 30 µl of histone extracts were resolved on 10% SDS-PAGE and transferred to a nitrocellulose membrane using the Semi-Dry Electroblotting Unit System (Sigma-Aldrich). Histone H3 and modified residues were detected with antibodies against H3 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... The covering cloth was removed from the microphone capsule and the follicle end of a whisker was attached directly to the very center of the exposed electret membrane with a nL-scale drop of a glue (Ethyl-2 Cyanoacrylate, Sigma-Aldrich). The glue covered less than 0.02mm2 area of the membrane to minimize possible membrane acoustic distortions (See inset in Fig.S1A) ...
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bioRxiv - Cancer Biology 2023Quote: ... 50µg of each protein sample was run on a 10% SDS-PAGE gel followed with a 90V transfer at 4°C for 90 minutes to a PVDF membrane (Millipore #IPFL00010). Membranes were blocked for one hour with Intercept PBS blocking buffer (LiCor #927-40000 ...
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bioRxiv - Bioengineering 2023Quote: ... Top channels (exposed membrane area ∼ 1.05 cm2) were filled with 30 µL of 50 µg/mL bovine plasma fibronectin (Sigma-Aldrich, F1141) at for 90 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: The deep-well plate with the drugs and communities was sealed with an AeraSeal breathable membrane (Sigma-Aldrich, cat. No. A9224) and incubated anaerobically at 37 °C for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... Microbial cells were obtained after filtering through 0.22-μm-pore-size polycarbonate membranes (45 mm diameter; Millipore, Bedford, MA, United States). The polycarbonate membranes with the collected microbial cells were cut into small pieces by using the sterile scissor ...