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Citations for Millipore Sigma :
7401 - 7450 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... Then equal amounts of total protein in each condition were resolved by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, ISEQ00010). The PVDF membrane was blocked with 1% (for anti-FLAG antibody ...
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bioRxiv - Microbiology 2020Quote: ... Equal amount of total protein was loaded and separated on 10% SDS-polyacrylamide gels and transferred to a polyvinylidene difluoride (PVDF) membranes (Millipore, IPVH00010). MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Microbiology 2020Quote: ... The same amount of protein was injected into a 10% SDS-PAGE gel for separation and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Germany). Cell membranes were blocked at room temperature for 2 hours with 5% skim milk and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated with 1:10,000 vol vol−1 secondary antibody anti-mouse horseradish peroxidase (HRP) conjugate (A5278, Sigma–Aldrich, USA) in 2% weight vol−1 BSA-TTBS ...
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bioRxiv - Neuroscience 2021Quote: ... 40μg of total proteins from each sample were separated by 8∼12% SDS-PAGE and transferred onto PVDF membranes (Millipore, USA), the membranes were blocked in 5% BSA ...
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bioRxiv - Microbiology 2021Quote: ... the alumina membranes were washed in sterile dH2O and incubated in 0.1 mg/ml poly-L-lysine (Sigma-Aldrich, Stockholm, Sweden) in dH2O for 5 minutes to prepare the surface for Matrigel coating ...
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bioRxiv - Microbiology 2020Quote: ... to obtain a final concentration of 80 % acetone (v/v) and pipetted on an Ultrafree-MC PTFE membrane 0.2 µm filter (Merck Millipore, Burlington, MA). The filter was incubated for 2 min at room temperature and was then centrifuged at 5000 x g for 1 min ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were transferred to a low fluorescent polyvinylidene difluoride membrane Immobilon-FL (0.45 μm pore size; Merck-Millipore, Czech Republic) by a Mini Trans-Blot® cell system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... each sample was filtered individually through a vacuum system under sterile conditions using a sterile membrane filter (pore size 0.22 μm, GV CAT No GVWP04700, Millipore, Darmstadt, Germany). The filters were collected and stored at −30 °C until processing ...
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bioRxiv - Microbiology 2020Quote: ... from the well were filter using a sterile membrane filter (pore size 0.22 μm, GV CAT No GVWP04700, Millipore, Darmstadt, Germany). The filters were collected and then resuspended in Dorn mineral medium [50] with methanol 25 mM as a sole source of carbon and energy ...
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bioRxiv - Microbiology 2021Quote: ... approximately 50 - 100 L of groundwater was filtered sequentially through 0.2 µm and 0.1 µm pore sized PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany; Table S1). With the exception of H32 (did not yield sufficient volumes) ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 µg of protein from each sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Merck Millipore, USA). Then ...
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma) overnight at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... membranes were washed (3×10 min) with TBS-T and incubated with the corresponding secondary antibody conjugated with peroxidase (Sigma-Aldrich) for 1 h at rt ...
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bioRxiv - Molecular Biology 2021Quote: ... and further concentrated 20-fold using Amicon Ultra Centrifugal Filter Units with a 10-kDa molecular mass cut-off membrane (Millipore Sigma) at 4°C to obtain ALF with a physiological concentration reported within the human lung (1 mg/mL of phospholipid) ...
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bioRxiv - Molecular Biology 2021Quote: ... of protein were separated by performing SDS-PAGE (10% or 12%) and then transferred onto polyvinylidene difluoride (PVDF) membranes (IPVH00005, Millipore, USA). Membranes were blocked for 1 hours with 5% skim milk at room temperature and then incubated overnight at 4 °C with the primary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling, pTYR 4G10 Millipore) and secondary 1h antibodies coupled to HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal volumes of protein samples were separated by 10% SDS/PAGE and electro-transferred to PVDF membranes (Millipore, Billerica, MA, USA). The immunoblots were probed with the indicated antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The crude protein was separated by 12% SDS-PAGE and then transferred to a nitrocellulose filter membrane (Millipore, Billerica, MA, USA) using a Trans-Blot Semi-Dry transfer cell (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 µg of whole-cell lysates was separated on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Millipore, Burlington, MA). The membranes were incubated with appropriate primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was blocked for 1 h with 5 % milk in TBST and blotted for anti-ssDNA (1:1000, Millipore, MAB3034).
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bioRxiv - Cancer Biology 2022Quote: ... The solution was neutralized using sodium acetate pH 7.0 and DNA sample was transferred to Whatman Hybond N+ Blotting Membrane (Millipore Sigma, Z761079). Nucleic acids were cross-linked by to the membrane by baking at 60 °C for 30 min followed by UV exposure ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were electrophoresed on 8-12% SDS-PAGE gels for approximately 60-70 min in SDS-PAGE running buffer and then transferred to an Immobilon-P polyvinylidene difluoride membrane (Millipore Corp.) using a MiniTransblot apparatus (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Collected blebs were then pelleted with a centrifugation at 16100 g for 5 min and incubated in a solution containing an exogeneous ATP regeneration system (energy mix) and alpha-toxin to permeabilise the bleb membrane (5% A-Hemolysin alphatoxin (1 mg/mL; H9395, Sigma Aldrich), 2% energy mix (50 mg/mL UTP ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated for 12 h at 4°C with the primary antibody: anti-VGlut1(AB5905, Millipore, Burlington, 1:2000), anti-PSD95 (51-6900 ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein samples were electrophoresed on 8% or 10% precast sodium dodecyl sulfate–polyacrylamide gel and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). The PVDF membranes were incubated with Tris-buffered saline containing Tween (TBS-T ...
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bioRxiv - Plant Biology 2022Quote: Five-day-old seedlings grown under the conditions described above were stained with the membrane-staining fluorescence probe FM4-64 at 4 μM (Sigma, Germany) for 10 min ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Molecular Biology 2022Quote: ... The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis and transferred onto Immobilon Transfer Membranes (Millipore, INYC00010) for immunoblotting ...
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bioRxiv - Microbiology 2022Quote: ... The membrane pellets were solubilized in 4 mL Wash buffer with 1% Triton 100-X (Sigma-Aldrich, Saint Louis, MO, USA). The solubilized membrane fraction was loaded on a column containing 0.5 mL Strep-tactin Superflow plus resin (50% suspension ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein (30 μg) were separated in a 10% SDS-polyacrylamide gel and then transferred to PVDF membranes (Millipore, USA). The membranes were blocked in 5% w/v bovine serum albumin before incubation with the primary antibodies at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... Equal amounts of proteins were electrophoresed on 12% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (PVDF, Millipore, USA). PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells that had migrated to the lower surface of the membrane were fixed with formalin and stained with crystal violet (Sigma-Aldrich). The migrating cells were examined microscopically and determined by counting the migrating/invasive cells in 5 randomly selected fields using an Olympus BX41 microscope (Olympus ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μg total proteins were loaded to the 8% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gels and transferred onto PVDF membrane (Millipore, IPFL00010) by electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... were seeded for 72 h on polycarbonate membrane Transwell permeable inserts (Costar, 3 μm pore size, 12 mm diameter, Sigma Ireland) and incubated for three days ...
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibodies: 1∶15000 rabbit anti-V5 antibody (SIGMA, Cat. # V8137), and 1:12000 mouse anti-α-Tubulin antibody (SIGMA ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... The lipid mixture was then resuspended in 1 ml HBSS, and suspensions were extruded using 100 nm polycarbonate membranes (Whatman, #800309) and a LiposoFast extruder device (Sigma-Aldrich) to generate large unilamellar vesicles.
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bioRxiv - Biophysics 2020Quote: ... The lipid mixture was extruded through a polycarbonate membrane with 100 nm pore size using an extruder set with holder/heating block (610000-1EA, Sigma-Aldrich). The lipid vesicle solution was stored at 4 °C and used within 2 weeks.
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bioRxiv - Microbiology 2021Quote: ... the supernatant was harvested by centrifugation at 10,000 x g at 4°C for 30 min and filter-sterilized using a 0.45 μm pore size hydrophilic PVDF membrane (Millex®-HV 0.45 μm, Merck Millipore, Darmstadt, Germany).
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bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated overnight at 4°C with an anti-GFP (ROCHE Anti-GFP, from mouse IgG1K, Millipore Sigma) (1:1000) ...
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bioRxiv - Physiology 2021Quote: ... Equal amounts of protein (5 μg) were subjected to 12.5 % SDS-polyacrylamide gel electrophoresis and blotted onto 0.2 mm PVDF membrane (Millipore, Billerica, MA, USA).
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bioRxiv - Immunology 2020Quote: ... Then the cell extracts mixed with 5 × SDS sample buffer were separated by 12 % SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Millipore, USA). Subsequently ...
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bioRxiv - Bioengineering 2021Quote: Proteins were separated on 9% or 12% SDS-PAGE gels in Tris-Glycine buffer and then were transferred into PVDF membrane (Sigma 05317). The blots were then blocked in 3% BSA (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Membrane was then washed 3x for 10 min with TBST and subsequently incubated with the alginate antibody (Sigma, monoclonal, anti-mouse) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: The edges of whole retinas were nicked to allow the retina to lay flat and placed on a nitrocellulose membrane (0.8 µm, Millipore, MA, USA) with the photoreceptor side up ...