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Citations for Millipore Sigma :
7551 - 7600 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and αCD133-scFv-DHFR2 fusion proteins were ordered from Integrated DNA Technologies (IDT) and cloned into the Novagen pET28a(+) vector (EMD Millipore, Cat: 69864-3) via NcoI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The fractions containing GRP78 protein were combined and concentrated by centrifuging through 30-kDa Amicon ultra-15 centrifugal filter units (EMD Millipore, Billerica, MA, USA). A solution of GRP78 protein was prepared at the concentration of 6 mg·ml-1 in 50 mM NaHCO3 buffer for the binding experiments.
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bioRxiv - Molecular Biology 2021Quote: ... 500μg of lysates in 500 μL of SEC buffer were incubated for 3 hours with primary antibodies directed to either AGO proteins (WAKO anti-AGO2 #011-22033, EMD Millipore anti-panAGO #MABE56) or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... followed by donkey anti-rabbit DyLight405 (Jackson Immuno Research 711-475-152) or neuron nuclei protein marker (NeuN, neuronal marker, guinea pig anti-NeuN, EMD Millipore ABN90, ON, Canada) followed by donkey anti-guinea pig (CF405M ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed 3 times with PBST and the protein bands were visualized by enhancing TMB substrate solution (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2022Quote: ... neurons from frozen spinal cord sections were stained by incubation with the specific neuronal marker anti-neuronal nuclei protein (NeuN, 1:500; Merk Millipore cat#MAB377, RRID: AB_11210778) diluted in blocking solution (PBS with 0.1% BSA and 0.2% Triton X100) ...
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bioRxiv - Immunology 2022Quote: ... and then lysed for 15 min with a M-PER Mammalian protein extraction reagent (Pierce Biotechnology, Rockford, IL) containing a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and 1 mM p-amidinophenyl methanesulfonylfluoride (PMSF) ...
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bioRxiv - Cancer Biology 2022Quote: ... 300 ug of protein in 600 uL of lysis buffer was used for the GTP pulldown with 20 ul of PAK beads (Sigma, Cat. no. 14-325) incubated for one hour ...
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bioRxiv - Neuroscience 2019Quote: ... 50 µl dynabeads Protein G (ThermoFischer Scientific, Waltham, MA, USA) were blocked using albumin from chicken egg white (Sigma-Aldrich, St. Louis, MO, USA) while rocking for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Biophysics 2020Quote: ... The following globular proteins were used to fit a standard curve of elution profile versus hydrodynamic radius: thyroglobulin 86 Å (Sigma-Aldrich, Cat #T9145) bovine serum albumin–dimer 45.6 Å and monomer 36.2 Å (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... 20µg of protein of each sample was separated on polyacrylamide gels at 160V for 80 minutes and blotted onto PVDF-membranes (Millipore, 80mA/membrane, 90 minutes). After blocking for 2h in Tris-buffered saline with Tween-20 containing 5% nonfat dry milk and/or BSA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2021Quote: ... sBHI media controls or Porphyromonas cell-free supernatants (240 µg of total protein) were incubated with 120 µg of human fibrinogen (Sigma-Aldrich, St. Louis, MO). Reaction mixtures were incubated at 37°C under anaerobic conditions or in an atmosphere of 5% CO2 for cell suspension or cell-free supernatants ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the target protein were concentrated to 2 mL using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa molecular weight cut-off (MWCO)) and injected into a gel filtration column (HiLoad 16/60 Superdex 200 ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed or tissue mechanically homogenised in lysis buffer (Extraction buffer, FNN0011) and protein content was quantified by a BCA assay (Sigma-Aldrich, St. Louis, MO). Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Western blots were treated with antibodies against the appropriate proteins followed by a second reaction with anti-mouse or anti-rabbit IgG conjugated to horseradish peroxidase (Sigma, St. Louis, MO, USA). The SuperSignal West Pico and Femto substrate kits (Pierce ...
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bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
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bioRxiv - Genomics 2021Quote: ... the aliquot of sheared chromatin fragments were incubated with antibody conjugated Protein A Dynabeads using total histone 3- (unmodified H3 antibody, Millipore #05-499; 1:1000) or histone 4- (unmodified H4 antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred to polyvinylidenedifluoride membranes that were blocked in 5% non-fat milk or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in Tris-buffered saline with 0.1% Tween 20 (TBS-T) ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... Supernatant equivalent to ∼100 mg total protein was incubated with 200 µl (packed) resin of anti-FLAG M2 affinity gel (Sigma, catalog # A2220, lot # SLCH0130) for 2 h on a rotator at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and 7.5 ml of heavy and light protein extracts were prepared and incubated with 40 μl EZview red anti-Myc affinity gel (Sigma-Aldrich, Cat. E6654-1ML) at 4°C for 4 h ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µg equivalent of protein from each sample were then treated with 5 mM tris (2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich Cat No: C4706) to reduce disulfide bonds and the resulting free cysteine thiols were alkylated with 10 mM chloroacetamide (Sigma Aldrich Cat No ...
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bioRxiv - Physiology 2023Quote: ... Free-floating brain sections were incubated at 4°C overnight with mouse anti-Glial fibrillary acidic protein (GFAP, 1:1000, Sigma-Aldrich, Saint-Louis, USA) or mCherry (ab125096 ...
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bioRxiv - Neuroscience 2022Quote: ... the expression of truncated tau protein was induced by the cultivation of cells in a medium without doxycycline (Sigma-Aldrich, St. Louis, MO, USA) for 3 days before cell seeding into co-culture ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled RNAs in the eluate were then detected by autoradiography and proteins were subjected to WB using an anti-Flag antibody (Sigma-Aldrich F3165; 1:10000).
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Neuroscience 2023Quote: Inflammatory proteins were measured using the MILLIPLEX Mouse Cytokine/Chemokine Magnetic Bead Panel – Premixed 32 Plex – Immunology Multiplex Assay (EMD Millipore. Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
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bioRxiv - Neuroscience 2024Quote: ... cells were stimulated with 1 µg/ml HERV-W ENV protein (Protein’eXpert, Grenoble, France) or 100 ng/ml lipopolysaccharide (LPS; Sigma-Aldrich, St. Louis, MO, USA) and respective buffer solutions (ENV buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Cell Biology 2023Quote: ... and protein was extracted in a modified RIPA buffer containing protease and phosphatase inhibitors (P8340 and 524624, respectively; Sigma-Aldrich, St. Louis, MO, USA). Proteins were detected by immunoblotting using standard techniques ...
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore; Thermo Fisher Scientific). PVDF membrane was treated with primary and secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Proteins from total cells extracts were quantified using bicinchoninic acid (BCA) reagent according to manufactured instructions (Sigma-aldrich Humeau, La Chapelle-Sur-Erdre, France). Protein extracts were treated with Leammli buffer ...
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bioRxiv - Neuroscience 2023Quote: ... cells from mixed glial cultures were collected and protein was digested using a lysis buffer containing Triton X-100 (Sigma Aldrich, St. Louis, MO) and supplemented with protease and phosphatase inhibitors (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Car-rigtwohill Co. Cork TRL). Concentrated proteins were aliquoted and stored at -80°C in sizing buffer.
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bioRxiv - Immunology 2023Quote: ... The concentrated proteins were then further purified through size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (Sigma-Aldrich GE28-9909-44). Selected fractions resulting from the size-exclusion run were pooled together and concentrated again for later use.
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bioRxiv - Molecular Biology 2023Quote: ... the protein solution was dialyzed overnight at 4°C in tubes of the Pur-A-LyzerTM Maxi 6000 Dialysis Kit (Sigma, cat. No. PURX60100-1KT) against dialysis buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 2 mg equivalent of protein from each sample was then reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich Cat No: C4706) for 30 minutes at room temperature and alkylated with 10 mM chloroacetamide (CAA ...
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bioRxiv - Molecular Biology 2023Quote: ... reared on SiGMV or CuLCrV infected sida or cucurbit plants were homogenized in 600 □l of CytoBuster protein extraction reagent (Millipore Sigma, Burlington, MA, USA) with 1X protease inhibitor cocktail (Cat#G6521 ...
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... in combination with anti-Ca2+/calmodulin-dependent protein kinase II α (CKII; monoclonal mouse IgG1, 05-532, clone 6G9, EMD Millipore Corporation, Temecula, USA), diluted 1:4000 ...
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bioRxiv - Plant Biology 2023Quote: ... and SIX6 with an internal TEV protease cleavage site for pro-domain removal were processed with 2 to 4 units of thrombin from bovine plasma (600-2,000 NIH units/mg protein) (Sigma-Aldrich Inc., St. Louis, USA) per mg of protein at 4°C or TEV protease (produced in-house ...
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bioRxiv - Biophysics 2023Quote: ... Purified protein was diluted to the concentration of 0.2 μg/ml and then incubated with Trypsin-agarose (Sigma-Aldrich Chemie GmbH, Buchs, SG Switzerland) for 2 h under 4°C temperature ...
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bioRxiv - Physiology 2024Quote: ... Biotinylated proteins were eluted by incubation with 2x SDS-PAGE sample buffer containing β-mercaptoethanol (100 µmol/L, Cat. # M6250; Sigma-Aldrich, Darmstadt, Germany) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Each lysate containing the same amount of proteins was separated on 8–12% SDS-polyacrylamide gels (PAGE) and electro-transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA, #IPVH00010). The membrane was incubated at 4°C overnight with the following primary antibodies ...