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Citations for Millipore Sigma :
7351 - 7400 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The HOS-TMPRSS2 cells that stably express Rhinolophus bat ACE2 were generated as described below (see “Generation of HOS-TMPRSS2 cells stably expressing a variety of ACE2 proteins” section) and maintained in Dulbecco’s modified Eagle’s medium (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... SG13009 colonies containing the appropriate species D pQE fiber knob protein expression vectors were cultured to an optical density of 0.6 in Terrific Broth (TB, Sigma-Aldrich, Gillingham, UK), harvested by centrifugation and purified by nickel immobilised metal affinity chromatography (IMAC ...
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bioRxiv - Microbiology 2023Quote: Proteins in the cell and viral lysates (5 μg of total protein and 10 ng of p24 antigen) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat# IPVH00010). Membranes were blocked with 5% milk in PBS containing 0.1% Tween 20 (0.1% PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Western immunoblotting to confirm protein expression was performed as stated above using anti-FLAG HRP-conjugated antibody (Sigma Aldrich, A8592; 1:15,000) to verify transfection and expression of FLAG-RGS14.
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bioRxiv - Biophysics 2023Quote: Denaturing MP experiments were carried out by incubating first the samples to a protein concentration of 100-400 nM in 5.4 M Urea (Sigma, Saint-Louis, USA) or 6 M Guanidine (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... 500 nM GBP2FL or 300 nM GBP2GD protein (wild type or mutant) was mixed with 200 µM GTP (Sigma, Cat No. G8877) in a reaction buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... the protein was diluted tenfold into temperature-equilibrated deuterated buffer made by lyophilizing 1x PBS and resuspending in D2O (Sigma-Aldrich 151882). Samples were quenched at each time point (15s ...
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bioRxiv - Bioengineering 2023Quote: ... ProteoExtract® native membrane protein extraction kit and 10 kDa Amicon® Ultra-15 centrifugal filters were purchased from Millipore Sigma (USA). Assays for measuring cell toxicity were purchased from Promega (Madison ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were resuspended in cold Ripa buffer supplemented with 1% n-Dodecyl-ß-D-maltoside and complete ethylenediaminetetraacetic acid (EDTA)-free protein inhibitors (Sigma-Aldrich), and incubated at 4°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were separated by SDS-PAGE using 10% agarose gel and transferred to transfer membranes (Millipore-Sigma, St. Louis, MO, USA), which were incubated in 5% nonfat milk (A600669 ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were separated by SDS-PAGE using 10% agarose gel and transferred to transfer membranes (Millipore-Sigma, St. Louis, MO, USA), which were incubated in 5% nonfat milk (A600669 ...
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bioRxiv - Physiology 2023Quote: ... The precipitated protein samples were resuspended in 100 µl of 100mM triethylammonium bicarbonate (TEAB) buffer pH 8.5 (Sigma-Aldrich cat no - T7408).
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bioRxiv - Plant Biology 2023Quote: ... 100 ng purified recombinant CTR1-KD or 1 µg MPK4 was mixed with 2 µg Myelin Basic Protein (MBP, a common kinase substrate, Sigma Cat. M1891) and 1 mM ATPγS in kinase reaction buffer (10 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged for 10 min (relative centrifugal force 12 879 g) and protein concentration was measured using the Bradford reagent (Sigma-Aldrich, USA). A protein calibration curve was determined with bovine serum albumin dissolved in a lysis buffer ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Genomics 2023Quote: ... the anti-TOMM20 antibody and the ConA protein were functionalized for click chemistry with the addition of a NHS-SS-DBCO (DBCO) (Sigma Aldrich #761532) molecule that subsequently reacted with the azide groups linked to their respective fluorophores ...
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bioRxiv - Biochemistry 2022Quote: ... for knockouts)37 containing the Che9c phage RecT annealase and Bxb1 integrase was electroporated into electrocompetent Msmeg cells (mc2155 strain73) and protein expression was induced with 500 ng/mL anhydrotetracycline (ATc, Sigma, cat. #31741). For chromosomal tagging ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted protein was concentrated in an Amicon Ultra-15 50 KDa molecular mass cut-off centrifugal filter unit (Millipore, Burlington, MA, USA) and further purified by SEC on a Superdex 200 Increase 10/300 GL (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Disulfide formation was determined by injecting protein at 1.5 mg/mL in the presence and absence of 50 mM TCEP-HCl (Millipore Sigma 646547-10X1ML) and detecting the mass shift.
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Genomics 2023Quote: ... untethered Dam and Dam-Cbx1 constructs were fused to an ERT2 domain so that the fusion protein would be translocated to the nucleus upon 4-Hydroxytamoxifen addition (4-OHT, Sigma, Cat#SML1666) (Extended Data Table 1).
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bioRxiv - Immunology 2023Quote: ... The eluted proteins were buffer exchanged into PBS and then concentrated using Amicon® 30 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9030).
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bioRxiv - Biochemistry 2023Quote: Tardigrade proteins were extracted using a modified acid extraction.35 Tardigrades were incubated in concentrated trifluoroacetic acid (Sigma Aldrich, St. Louis, MO) for 40 min before neutralizing to approximately pH 7 with 2 M Tris (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Glycine and Tris-HCl in the protein solution were replaced with PBS three times using centrifugation with Amicon Ultra-15 Centrifugal Filter Unit (50K) (Millipore, Cat# UFC905024). Protein concentrations were determined using a NanoDrop spectrophotometer (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Proteins were concentrated and exchanged with phosphate-buffered saline (PBS) pH7.4 using an Amicon YM-10 centrifuge concentrator (Millipore, Bed-ford, MA).
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bioRxiv - Plant Biology 2023Quote: ... Supernatants containing soluble proteins fraction were transferred in a new Eppendorf tube of 1.5mL and the total protein concentration was determined by BRADFORD assay (Sigma-Aldrich, ref 23238).
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... SDS samples containing 4∼8 μg total proteins were separated with 12% HEPES-Tris PAGE gel (MEILUNBIO, MA0243) and transferred to polyvinylidene difluoride membranes (Millipore, 70584-3). The membrane was blocked with 5% skim milk in TBST buffer (20mM Tris ...
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bioRxiv - Immunology 2023Quote: ... To ensure efficient and reproducible protein immobilization a preliminary array validation was carried out with anti-FLAG antibodies (Sigma-Aldrich, cat# F7425) 1:5000 and mouse anti-His6 tag polyclonal antibodies (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The prepared proteins were separated by SDS-PAGE gel electrophoresis (Yazyme, Shanghai, China) and transferred to activated PVDF membranes (Millipore, MA, USA). The PVDF membranes were then gently rinsed with TBST and placed in 7% skim milk powder solution and closed on a shaker at room temperature for 3 h ...
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bioRxiv - Pathology 2023Quote: ... An equal amount of protein (10 µg) was subsequently separated by 12% SDS-PAGE and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Bedford, MA). The membrane was blocked with a 5% skimmed milk solution prepared in Tris-buffered saline containing 0.1% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The viral pellet was then lysed in Western blot lysis buffer with 4x Protein Sample Loading Buffer (LICOR) supplemented with 10% β-mercaptoethanol (Sigma Aldrich) and heated at 95°C for 5 min.
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 40 μl of Protein A/G magnetic beads was incubated with 5 μg anti-pan-ADP-ribose binding reagent (Millipore Sigma, MABE1016) per IP sample in 100 μl PBS for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates with anti-FLAG antibody were then incubated for additional 30 min (4°C, rotating) with 75 µl/sample pre-washed (3 times in buffer A) Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Afterwards ...
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
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bioRxiv - Neuroscience 2023Quote: ... For each lane we treated 15-20 μg protein samples with 4X NuPAGE LDS Sample Loading buffer (Thermo #NP0007) and 5% 2-mercaptoethanol (Sigma #M3148-25ml), heated them at 70°C for 6 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 hours at RT and then immunoblotted overnight at 4°C with the primary mouse anti-amyloid precursor protein A4 antibody (clone 22C11) (Sigma MAB348-AF647) (1:3000 ...
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bioRxiv - Microbiology 2023Quote: ... active and inactive NS2B(H)-NS3 pro proteases were generated as described previously and cloned into the MCS1 (multiple cloning site 1) of dual protein expression plasmid pETDuet-1 (Novagen, Beijing, China) through restriction sites EcoRI and NotI ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were visualized by Coomassie-stained SDS-PAGE and concentrated using a 3 kDa MWCO Amicon Ultra centrifugal filter (Millipore Sigma, #UFC9003,).
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bioRxiv - Immunology 2024Quote: ... Proteins from total cell extract were quantified using BCA reagent according to manufacturer instructions (Sigma-Aldrich, Humeau, La Chapelle-Sur-Erdre, France). A standardized amount of protein extracts was treated with Laemmli buffer containing 10 mM DTT and heated at 95 ° C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 15µg of each protein sample was prepared with Laemmli Sample Buffer (Bio- Rad cat. 1610737) and 50mM DTT (Millipore Sigma cat. 43816). Samples were loaded on a 4-15% Criterion TGX Protein Gel (Bio-Rad cat ...
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bioRxiv - Biophysics 2024Quote: ... γ-cyclodextrin (γ-CD, CAS#7585-39-9), C-reactive protein (CRP, AG723) and human serum (H4522-100ML) were ordered from Sigma-Aldrich. 1 µM γ-CD stock suspension was prepared with MilliQ ...
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bioRxiv - Neuroscience 2024Quote: ... 450 µg of total protein were incubated for 20 min (rotating wheel, RT) with 50 µL of streptavidin-agarose beads (Sigma-Aldrich, #S1638). After incubation ...