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Citations for Millipore Sigma :
7451 - 7500 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was measured using THERMO Labsystems Multiscan EX at wavelength 570 nm with a Bradford Reagent (Sigma Life Science cat no. B6916) and a bovine serum albumin standard (Thermo Scientific cat no ...
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bioRxiv - Immunology 2019Quote: ... Chromatin (5 μg) was used for immunoprecipitation reactions on Protein A beads with 0.5 μg of polyclonal antibodies for H3K4me1 (Millipore Sigma Cat. 07-436), H3K4me2 (Millipore Sigma Cat ...
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bioRxiv - Biophysics 2019Quote: ... reduced and alkylated protein extracts were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, # MRCPRT010 Ultracel YM-10). Digested samples were desalted using ZipTips according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The anti-Phosphoepitope SR proteins (Cat#MABE50; clone 1H4) and anti-SRp40 (Cat#06-1365) antibodies were purchased from Millipore Sigma (Temecula, CA) and used at 1:500 dilution ...
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bioRxiv - Immunology 2020Quote: ... a pooled rhesus serum collected day 14 after infection with SARS CoV-2 was used as standard after depletion of IgG using GE Healthcare Protein G Sepharose (Sigma, St. Louis, MO) as described (52) ...
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bioRxiv - Cell Biology 2020Quote: ... The precipitated proteins were centrifuged for 10 min at 18,000 g and the protein pellet resuspended 50 mM NH4HCO3 before digestion with sequencing grade porcine trypsin (enzyme to protein ratio of 1:40) (Sigma-Aldrich, MO, USA) for 16 hours at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... 20 μL of either eIF4GI or DAP5 capture antibodies were incubated with PureProteome™ Protein A/G mix magnetic Beads (EMD Millipore Corporation) at room temperature with continuous mixing for 1 hr ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS- PAGE and those containing pure tag free CBX8 protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer consisting of 20mM MES pH 6.5 ...
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bioRxiv - Synthetic Biology 2021Quote: The N-terminal GST-tag was removed from CBX8 proteins by TEV protease cleavage according to manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer containing 25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA). After blocked with 5% non-fat milk ...
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bioRxiv - Genomics 2019Quote: ... Approximately 25μg of proteins were analyzed on 10% SDS-PAGE followed by western blots using monoclonal anti-FLAG-M2-Peroxidase (HRP) (Sigma Aldrich, 1:2000) and anti-β actin (1:4000).
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bioRxiv - Cancer Biology 2019Quote: ... Where indicated immunoprecipitates were treated with RNase A (100 µg per mg of protein lysate used for immunoprecipitation; Sigma-Aldrich, St. Louis, MO) (56) ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Physiology 2021Quote: ... Protein extraction from the remaining EDTA-insoluble pellet was performed with a 4M guanidine hydrochloride (Gu-HCl, Sigma-Aldrich St. Louis, MO, USA) solution at pH 7.4 containing protease inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then clarified by centrifugation and the protein content of each supernatant was determined using the Quanti-Pro bicinchoninic acid (BCA) assay kit (Sigma-Aldrich, MO, USA). Equal protein amounts (100 μg ...
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Bioengineering 2020Quote: ... endothelial cells were enzymatically removed and subsequently epithelial cells were lysed with a protein lysis buffer (MSD, R60TX-3) containing protease (ThermoFischer, 78425) and phosphatase inhibitors (Sigma-Aldrich, P0044 & P5726). Protein extraction was performed according to standard protocols and the quantification of the total amount of protein/ sample was done by Bradford assay (Thermo Fischer ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Biochemistry 2020Quote: A 10 μM solution of purified PAPR10-CD protein was incubated for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Neuroscience 2021Quote: ... 2.5 ug of each protein sample in 50 μL of PB lysis buffer with PMSF cocktail inhibitor (Sigma-Aldrich, St. Louis, MO, USA) was spotted on nitrocellulose membrane and washed 2X with TBS by vacuum filtration ...
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bioRxiv - Neuroscience 2022Quote: ... The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting using the following antibodies: anti-HA (Sigma, H3663; clone 12CA5, 1:1000), anti-Flag (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: Samples were isolated from whole brain as described above and proteins were precipitated from lysis buffer using Trichloroacetic acid (TCA) as follows: 50μl of 100% TCA (Sigma/Millipore – cat# T6399-5G) was added to 200 μl of protein sample at a final concentration of 20% ...
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bioRxiv - Physiology 2022Quote: ... The protein extracts were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon membrane (EMD Millipore, Billerica, MA). The membrane was blocked with 5% nonfat milk for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... alkylated proteins were digested using Lysyl Endopeptidase® (FUJIFILM Wako Pure Chemical Corporation, 125-05061) and sequencing grade modified trypsin (Sigma, TRYPSEQM-RO) followed by quenching with 1% trifluoroacetic acid ...
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bioRxiv - Cancer Biology 2022Quote: Cells were harvested and pelleted for protein extraction using a cocktail consisting of 1X RIPA Lysis and Extraction Buffer (EMD Millipore 20-188), 5% glycerol ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were separated on 8% SDS-PAGE gels and transferred to a PVDF membrane (immobilon FL; Sigma-Aldrich, St Louis, MO, USA). Membranes were blocked with 1% (for TMEM16A protein ...
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bioRxiv - Plant Biology 2022Quote: ... 20 mM Tris–HCl [pH 8.0], 5 mM imidazole, 5% glycerol, 1 mM PMSF, 1/10 protein inhibitor cocktail: Sigma, St. Louis, MO, USA). After centrifugation for 40 min at 21,000 xg ...
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bioRxiv - Plant Biology 2022Quote: ... Various recombinant TFIIIA-7ZF proteins with an intein-chitin binding domain (CBD) tag were overexpressed using the Escherichia coli BL21(DE3) Rosetta strain (EMD Millipore, Burlington, MA). For each construct ...
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bioRxiv - Immunology 2022Quote: All protein was extracted from ear skin samples by homogenizing tissues in RIPA buffer containing protease inhibitor cocktails (Sigma-Aldrich, St. Louis, MO). SCF levels were determined using ELISA kit (R&D Systems) ...
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bioRxiv - Microbiology 2022Quote: ... IgG assay samples were incubated with 100 μL of pre-washed 50/50 Protein Sepharose A slurry (Sigma-Aldrich, diluted in lysis buffer) for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: Total GLP1 protein in brain tissue or in plasma was determined using GLP-1 Total ELISA 96-Well Plate Assay (Sigma-Aldrich, EZGLP1T-36K). The assay was conducted according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... group was fed 5 mg artemia and 30 mg egg yolks from chicken (59% fat, 32% proteins, 2% carbohydrates; Sigma-Aldrich, MO, USA) for one or two months (Landgraf et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). The antibodies are listed in Supplementary Table 4 ...
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bioRxiv - Biophysics 2022Quote: ... The imidazole was removed from the purified protein using an Amicon Ultra centrifugal filter with a regenerated cellulose membrane (Millipore Sigma, Burlington, MA). At least 3 washes with imidazole-free PBS buffer were done ...
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bioRxiv - Cell Biology 2024Quote: ... Control conditions included a set of micropattterned grids that were not incubated in antibody (No Ab) and another set of micropatterned grids that were treated with monoclonal antibodies to VZV glyco-protein E (gE; EMD Millipore Cat# MAB8612).
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bioRxiv - Bioengineering 2024Quote: A vascular matrix was formed by co-encapsulating EC and MSC within a fibrin carrier (Sigma-Aldrich, 2.5 mg/mL final clottable protein) and casting the cell-laden construct into individual wells of a 48-well plate (250 µL/well) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Biophysics 2024Quote: ... Columns were then washed with 5 column volumes of K+-Tris buffer and protein as eluted using 2.5 mM d-desthiobiotin (Sigma-Aldrich, St. Louis, MO) in K+-Tris buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... the chromatin was precleared for 1hr at 4°C with pre-blocked ChIP-grade Protein A/G magnetic beads (Millipore Sigma, 16-663). The precleared lysate was divided into equal parts and immunoprecipitated using either anti-FLAG antibody (Sigma ...
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bioRxiv - Genetics 2024Quote: ... The sonicated DNA was then precleared with 50 µl of Protein A agarose beads/Salmon sperm DNA slurry (EMD Millipore, Catalog # 16–157) for one hour on a rotator in cold ...
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bioRxiv - Microbiology 2024Quote: ... a 10 μM solution of purified PAPR10-CD protein was incubated for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined by standard curve intensity measurements from Coomassie blue-stained bovine serum albumin (A7617, Sigma Aldrich, St. Louis, MO, USA) of known concentrations.