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Citations for Millipore Sigma :
7401 - 7450 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 25µg total lysate was removed as input and 0.5mg total protein was incubated with pre-washed magnetic M2 anti-FLAG (Sigma-Aldrich M8823-5ML) beads at 4°C for 1.5 hours while inverting ...
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bioRxiv - Molecular Biology 2024Quote: ... to alkylation with IAM 200mM and to complete protein digestion with 1 μg of Trypsin (Sigma-Aldrich Inc., St. Louis, MO, USA). The peptide digests were desalted on the Discovery® DSC-18 solid phase extraction (SPE ...
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bioRxiv - Immunology 2024Quote: ... These diluted test peptides were co-incubated with 0.1 μM biotin-labeled peptide and 50 nM purified MHC-II proteins (ProImmune, Oxford, United Kingdom) at 37°C for 24h in the presence of Octyl-β-D-glucopyranaside (Sigma-Aldrich). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... Pooled lysate was subsequently split into 2×200 μL aliquots and protein was enriched by adding 20 μL anti-flag (Sigma cat#M8823) or anti-myc (Fisher cat#PI88842 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pooled lysate was divided into two 350 μL aliquots and protein was enriched by adding 35 μL anti-flag (Sigma cat#M8823) or anti-pErk beads (see ‘Basal Activity Scan’ for instructions on generating pErk beads) ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was cut across the molecular mass region of the corresponding proteins and separately probed with the following corresponding antibodies: an anti-β-glucuronidase (N-terminal) antibody (G5420, Sigma‒Aldrich) and an anti-tubulin antibody (T5168 ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were visualized by Coomassie-stained SDS-PAGE and concentrated using a 3 kDa MWCO Amicon Ultra centrifugal filter (Millipore Sigma, #UFC9003,).
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bioRxiv - Physiology 2024Quote: ... 20 µg total protein extracts were separated by 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and cut into 2-3 parts ...
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bioRxiv - Genomics 2024Quote: ... Protein was purified by Nickel-NTA resin and treated with TEV protease (1mg lab made TEV per 40mg of protein) and benzonase-100units/ml (Novagen, 70664-3) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluates were resolved on a 10% SDS-PAGE gel and the proteins were electroblotted onto PVDF membranes (Millipore, Immobilon-P, Cat # IPVH20200) for probing with following primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Twenty micrograms of total protein were separated on 10% SDS- PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore Co., Bedford, MA). Protein transfer was checked by staining the membrane with Ponceau S red (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... kinase assays were performed by incubating the immunoprecipitated proteins and beads in 30 μl kinase buffer supplemented with 50 μM ATP and 3 μg histone H1 (EMD Millipore, 14-155) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... An even protein transfer was verified by reversibly staining membranes with Poncaeu S solution (Sigma-Aldrich, 0.1% w/v in 1% acetic acid). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein was separated by 12% sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) electrophoresis and then was transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA), which was followed by incubation with antibodies against STAP1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-10 μg protein lysates were cultured and proteasome activity measured using the 20S Proteasome Activity Assay kit (APT280, Millipore, Billerica, MA, USA) per manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2019Quote: ... cells were harvested between 24 and 48 h post-transfection and lysed with M-PER Mammalian Protein Extraction Reagent (Pierce Biotechnology) containing a mammalian protease inhibitor cocktail (SIGMA P8340, Sigma-Aldrich) according to the protocol of the manufacturer ...
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bioRxiv - Cancer Biology 2019Quote: ... A total of 70 μg of protein was subjected to electrophoresis and then transferred to nitrocellulose membranes (Sigma Chemical Co., St. Louis, MO). The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal ...
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bioRxiv - Physiology 2019Quote: ... equal amounts of protein (in 1X Laemmli loading buffer) were resolved using SDS-PAGE gels and transferred to PVDF membranes (Millipore Cat. No# IPVH00010). After blocking in 5% BSA or 5% fat-free milk in TBST (TBS with 0.1% Tween-20 ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were induced to express the indicated BioID2-tagged proteins by the addition of 0.2 μg/ml doxycycline (Sigma-Aldrich, St. Louis, MO) for 16 hours ...
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bioRxiv - Bioengineering 2020Quote: ... cells were pelleted by 10 minutes of centrifugation (4122 RCF, 4 degrees C) and lysed with BugBuster protein extraction reagent (Millipore Sigma, Catalog #70584) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used for immunofluorescence on whole-mounted retinal sections were brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID:AB_94166) at 1:1000 and GFAP (1:1000) ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: Cell culture supernatant was diluted 1:1 in binding buffer (0.16 M sodium phosphate, 500 mM NaCl, pH 9) and loaded on a Protein A Sepharose (Sigma Aldrich, 17-1279-03) column at a flow rate of 2 ml/min ...
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bioRxiv - Microbiology 2021Quote: ... Buffers were then exchanged to PBS and proteins were concentrated by 30 kDa cut-off Amicon Ultra-15 Centrifugal Filter Units (Millipore, Cat. No. UFC903096).
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bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (30 μg) from the cell lysate were resolved by SDS–PAGE and transferred to Immobilon-P membranes (Millipore Corp., Billerica, MA, USA). Membranes were incubated with anti-NLRP3 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... and screened for expression of the fusion proteins by western blotting with an anti-FLAG M2-HRP antibody (Sigma, mouse monoclonal, cat#: A8592).
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bioRxiv - Biochemistry 2021Quote: ... For the time course shown in Fig 2B 19 μl of this protein were combined with 1 μl of trypsin (Sigma; 1 mg/ml) and the mixture was incubated at RT ...
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bioRxiv - Bioengineering 2021Quote: ... The dried silk fibroin protein was then solubilized by denaturation in 9.3 M aqueous lithium bromide (Catalog No. 213225, Sigma Aldrich, St. Louis, MO) at 60°C for 4 hours ...
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bioRxiv - Cell Biology 2021Quote: ... The immunoprecipitates on protein A agarose or sepharose were either boiled directly in sample buffer or eluted with FLAG peptide (Sigma, 200 μg/mL) or HA peptide (Roche ...
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bioRxiv - Systems Biology 2020Quote: ... on day 0 with a 50 µl volume of Escherichia coli O111:B4 LPS purified by ion exchange and with <1% protein and <1% RNA (Sigma Aldrich; catalog L3024), which was diluted in sterile ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Immunology 2021Quote: ... The protein was dialyzed against PBS and subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL (Sigma, GE Healthcare product) column on an AktaTM Pure (GE ...
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bioRxiv - Plant Biology 2021Quote: ... Aliquots were run on a 4–12% SDS-PAGE gradient gel and electrophoresed proteins were transferred to a PDVF membrane (Immobilon-P, Millipore, Burlington, MA, USA). To facilitate detection ...
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bioRxiv - Molecular Biology 2021Quote: ... the antibody – lysate mixture was incubated at 4°C with rotation for 1 hr prior to the addition of protein A/G agarose beads (Millipore, 50 L/IP) and continued overnight incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblotting for specific proteins was carried out using the following primary antibodies: Mouse monoclonal antibodies against anti-FLAG M2 (Millipore Sigma, Billerica, MA), Jak1 (CST-50996) ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal volumes of protein samples were separated by SDS-poly acrylamide gel electrophoresis and electro-transferred to PVDF membranes (Millipore, Billerica, MA, USA). After blocked with 5% non-fat milk dissolved in TBST (10mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting protein was further concentrated (Amicon Ultra 0.5 mL and 4 mL Centrifuge filters, 30 KDa NMWL devices, Millipore Cat#UFC505024 and UFC805024) and flash frozen in liquid nitrogen for future use.
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bioRxiv - Neuroscience 2022Quote: ... The resulting protein was concentrated (Amicon Ultra 0.5 mL and 4 mL Centrifuge filters, 30 kDa NMWL devices, Millipore Cat#UFC505024 and UFC805024) and flash frozen in liquid nitrogen for future use.
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bioRxiv - Plant Biology 2022Quote: ... normalizing protein levels to the Rubisco large subunit visualized in membranes stained with Ponceau (0.1 % Ponceau S Sigma-Aldrich in 5 % acetic acid). The statistical differences between the average protein levels of each sample were inferred using a Student’s t-test.
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bioRxiv - Microbiology 2022Quote: ... THP-1 cells were harvested and washed twice with PBS and lysates were prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... BG-oligonucleotide-labeled SNAP proteins bound to resin were again washed with wash buffer and eluted using 0.2 mg/mL FLAG peptide (Sigma-Aldrich, St. Louis, MO) overnight at 4°C with rotation ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Plant Biology 2021Quote: ... total soluble proteins were extracted from 50 mg of ground frozen shoot material with 100 µl 2× Laemmli buffer (#S3401, Sigma-Aldrich, Steinheim, Germany). Proteins were denatured and separated as described above ...
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bioRxiv - Cancer Biology 2019Quote: CAF cell monolayers were lysed for protein with RIPA buffer containing protease inhibitor cocktail at 1:100 dilution (both Sigma-Aldrich, Dorset, UK). Lysed cell suspensions were quantified for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was normalized for all tissues with RIPA buffer and 2x SDS sample buffer containing β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO) was added ...
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bioRxiv - Neuroscience 2021Quote: ... Protein signal was developed using enhanced chemiluminescence according to the manufacturer’s instruction (Immobilon® Forte Western HRP substrate, Merck Millipore Ltd., VIC, Australia) and visualised using the ChemiDocTM XRS system (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... equal amounts of protein extracts were subjected to separation SDS-PAGE before being transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA), and subsequent visualization by Western blotting with distinct antibodies as indicated ...