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Citations for Millipore Sigma :
7501 - 7550 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined by standard curve intensity measurements from Coomassie blue-stained bovine serum albumin (A7617, Sigma Aldrich, St. Louis, MO, USA) of known concentrations.
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Immunology 2023Quote: ... Cell lysates (intracellular RBD and N protein) were generated by processing the cell pellets in a suitable volume of RIPA buffer (Sigma Aldrich cat# R0278) according to the manufacturer’s recommendations.
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bioRxiv - Microbiology 2022Quote: ... Iron concentrations were then normalized to the protein content in each sample using the bicinchoninic acid (BCA) assay (Sigma Aldrich, St. Louis, MO). Quantification was performed from 3 independent cultures for each strain.
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bioRxiv - Neuroscience 2023Quote: Whole retinas were flat-mounted on slides coated with Gatenby’s solution and stained sequentially using the following primary antibodies: brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID: AB_94166) at 1:1000 ...
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bioRxiv - Immunology 2023Quote: Cells from day 5 bone marrow cultures from tet-LIN28B or littermate control mice were used for lysis and RIP using Magna RIP RNA-Binding Protein Immunoprecipitation kit (Millipore, cat#17-700) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets were resuspended in 200 µL lysis buffer (1X BugBuster® Protein Extraction Reagent, EMD Millipore; 0.025 U/µL Benzonase® Nuclease, EMD Millipore; 1X Halt™ Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... separate sets of sections were immunohistochemically processed using anti-glial fibrillary acidic protein (GFAP) monoclonal antibody 1:500 (G3893, Sigma, Saint Louis, Missouri), anti-doublecortin (DCX ...
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bioRxiv - Molecular Biology 2023Quote: ... The imidazole was removed from the purified protein using an Amicon Ultra centrifugal filter with a regenerated cellulose membrane (Millipore Sigma, Burlington, MA). At least 3 washes with imidazole-free PBS buffer were done ...
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bioRxiv - Physiology 2023Quote: ... We blocked sections in 5% normal goat serum in phosphate-buffered saline with 0.1% Tween 20 (blocking buffer) for 30 min at room temperature (RT) and then incubated in primary antibody for laminin protein (1:500, Sigma catalog no. L93931) overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Equal amounts of protein extracts were subjected to electrophoresis in 8–12% SDS- polyacrylamide gels and then transferred to PVDF membranes (EMD Millipore, Burlintgon, MA). The membranes were blocked with 5% non-fat milk at room temperature and then incubated with relevant primary antibodies at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The bands were destained using a 1:1 ratio of 25 mM ammonium bicarbonate (pH 8.5) and 50% acetonitrile followed by the reduction of the proteins using 20 mM dithiothreitol (Sigma, St. Louis, MO, USA) for one hour at 56°C ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Biochemistry 2022Quote: ... only C501 and C527 of C2-TRAP1 variant) were blocked by incubating proteins with 10 mM N-ethylmaleimide (NEM, Sigma-Aldrich, Missouri, USA) for 30 min at 37°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was detected using the Simple-Western-JESS capillary western blot system (Bio Techne) multiplexed for total protein and chemiluminescence detection of mouse αSFPQ (1:2000; Sigma-Aldrich WH0006421M2, RRID:AB_1843565) or mouse αTIAL1 (1:10 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein pellets were resuspended in 50 µL of 50 mM NH4HCO3 and digested for 16 h with trypsin (Sigma-Aldrich, Product code T6567) with an enzyme to protein ratio of 1:20 ...
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bioRxiv - Immunology 2023Quote: ... For purification of HDAC1-Flag 1 mg of protein was mixed with 120 µl of crosslinked M2-Flag beads (Sigma Aldrich, M8823-5ml) and incubated rotating overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
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bioRxiv - Neuroscience 2023Quote: Knockdown of proteins was achieved through RNA interference either by CaCl2 transfection of gene-specific shRNA encoding vectors (pLKO.1; Sigma-Aldrich or shRNAmiR) on DIV 7 or by transduction of cells with lentiviral particles harboring the gene-specific shRNA on DIV 2 ...
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bioRxiv - Plant Biology 2023Quote: ... were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™, Mini, EDTA-free, Sigma-Aldrich, 04693159001). Proteins were eluted from magnetic beads by boiling at 95°C for 10 min in 50 µl 2x Laemmli sample buffer ...
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bioRxiv - Neuroscience 2023Quote: Western blot analysis of MBP was performed on protein lysates from the anterior cerebrum at P22 as previously described [32] using rat anti-MBP (Millipore MAB386 1:500) and anti-β-actin (Sigma-Aldrich AC-74 ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins were resolved by electrophoresis on SDS-PAGE gels and transferred to a PVDF Immobilon-FL membrane (EMD Millipore, Catalog # IPFL00010). The PVDF membrane was incubated with blocking buffer (Rockland ...
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bioRxiv - Pathology 2023Quote: ... 20 μg total protein extract or of WGA-enriched proteins were separated using 4-15% SDS–PAGE gels and were transferred to nitrocellulose membrane (Millipore, Bedford, MA, USA). Blots were probed with primary antibodies and then developed with horseradish peroxidase (HRP)-enhanced chemiluminescence (WesternBright ECL ...
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bioRxiv - Genetics 2024Quote: Snap frozen gastrocnemius or spinalis muscles were homogenised in 4% SDS lysis buffer and assessed for total protein concentration using Direct Detect Assay free cards (Millipore, Cat #DDAC00010-GR). Proteins were separated by SDS-PAGE using stain-free precast midi- criterion gels (Biorad) ...
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bioRxiv - Developmental Biology 2024Quote: ... Puromycin incorporated into the proteins were detected in a Western blot using a mouse-origin anti-puromycin monoclonal antibody (12D10, Sigma MABE343, lot # 3484967) and a goat-anti-mouse HRP-conjugated secondary antibody (Abcam ab6789 ...
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bioRxiv - Biophysics 2024Quote: ... 10% v/v glycerol and 0.03% w/v DDM and 300 mM imidazole) and the eluted protein was concentrated to ∼500 μL using 100 kDa MWCO centrifugal filters (Millipore AmiconTM Ultra, Merck). The concentrated sample was then loaded onto a Superdex 200 10/300 gel filtration column (Cytiva ...
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bioRxiv - Molecular Biology 2024Quote: ... 30-60 µg protein extracts were fractionated using 8-10% SDS-PAGE under reducing conditions and transferred onto Immobilon-FL PVDF (Millipore, cat no# IPFL00010) membranes ...
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bioRxiv - Plant Biology 2020Quote: ... Thirty µg of total protein of each sample were separated on 15% SDS–polyacrylamide gels and transferred to ImmobilonTM PDVF membranes (Millipore Co., Bedford, MA, USA). The membrane was blocked for 1 h in PBS-T with 5% non-fat dried milk at room temperature ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed in PBS-T for 40 min and the bound proteins were visualized with anti-FLAG-HRP antibodies (1:10000 in PBS-T for 1h, Sigma-Aldrich, Cat. no: A8592). All steps were performed at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Primary antibodies raised against the following proteins were used in block buffer (5% BSA, 0.1% Tween-20, in PBS) at the indicated dilutions: α-Tubulin (Sigma Aldrich T5168, 1:50,000), FLAG (Agrisera AS15 2871 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The resistant populations were previously selected for high levels of resistance against protein synthesis inhibitors from the aminoglycoside family, gentamicin (GEN; Carl Roth, Germany; Ref. HN09.1) and streptomycin (STR; Sigma-Aldrich, USA; Ref. S6501-5G), or alternatively cell-wall synthesis inhibitors from the β-lactam family ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Cancer Biology 2021Quote: ... protein samples were separated on a sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred onto Immobilon-P PVDF membrane (EMD Millipore, Billerica, MA, USA). The membrane was incubated for 1 h in blocking buffer (Tris-buffered saline ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial mass was assessed by measuring citrate synthase activity from 4 million whole cells normalized to protein input (U/mg) in triplicate from three biological replicates using the Citrate Synthase Assay Kit (CS0720, Sigma-Aldrich, St, Louis, MO).
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bioRxiv - Cancer Biology 2020Quote: HEK293-T cells were transiently transfected with GFP-V5 or GREP1-V5 fusion proteins using OptiMem and Fugene HD (Sigma-Aldrich, St. Louis, MO). 72 hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatant equivalent to ~100 mg total protein was incubated with 200 μl (packed) resin of anti-FLAG M2 affinity gel (Sigma, catalog # A2220, lot # SLCH0130) for 2 h on a rotator at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The H2O2 concentrations were determined by a standard curve developed using 0.25 – 2.5 μM and normalized by calculating the amount of protein in the extract using a standard Bradford Assay (Bradford, 1976) with Bradford Reagent (Sigma-Aldrich, cat no. B6916).
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bioRxiv - Systems Biology 2021Quote: ... Cell-free extract preparation for yeast cells was done using YeastBusterTM Protein Extraction Reagent supplemented with 1% of 100x THP solution according to the description (Novagen, San Diego, CA, USA). To a pellet with a wet weight of 0.3 g ...
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bioRxiv - Systems Biology 2020Quote: ... The pooled reference mixture that consisted of an equal amount of protein from each sample was spiked with UPS2 Proteomics Dynamic Range Standard (Sigma-Aldrich, Saint-Louis, MO) and digested with trypsin ...
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bioRxiv - Neuroscience 2020Quote: ... Flat-mounted retinas were incubated overnight at 4°C with antibodies against RNA-binding protein with multiple splicing (RBPMS; EMD Millipore Corp., Burlington, MA) diluted 1:500 in blocking buffer (2% bovine serum albumin ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody was incubated at room temperature protected from light for 1 hour in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using IRDye® 800CW Goat anti-Mouse IgG secondary antibody at 1:10,000 (LI-COR Lincoln ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was incubated at room temperature for 2 hours in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using STAT1 1:1000 (#14994 ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained for bait proteins using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165; used at 1:2500), Rabbit-anti-Giantin antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Proteins that passed this quality control test were then labeled directly with NHS-tethered Cy5 dye (Catalog No. GEPA15101, Sigma-Aldrich, St. Louis, MO) on the glutathione beads ...
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bioRxiv - Cancer Biology 2021Quote: ... Denatured proteins in the supernatant were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (Millipore Corporation, Billerica, MA, USA) membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2022Quote: A gBlock Gene Fragment coding for DHFR2 – αB7-H3 scFv fusion protein was ordered from Integrated DNA Technologies (IDT) and cloned into the Novagen pET28a(+) vector (EMD Millipore, Cat: 69864–3) via the NcoI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2022Quote: The proteins were visualized by incubating membranes for 30 minutes in BCIP®/NBT Alkaline Phosphatase Substrate (B5655, SIGMAFAST™ BCIP®/NBT, Sigma-Aldrich). The intensities of the detected bands were analyzed with a FluorChem 8800 Imager (Alpha Innotech ...