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Citations for Millipore Sigma :
7301 - 7350 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Developmental Biology 2021Quote: The proteins in the sample were precipitated using methanol/chloroform and were resuspended in 6 M Guanidine HCl (Sigma cat# G3272-1KG), buffered using 50 mM EPPS (Alfa Aesar cat# A13714) ...
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bioRxiv - Biochemistry 2020Quote: ... The column was washed with 10 column volumes of wash buffer and proteins were eluted with 8 ml of the same buffer containing 10 mM maltose (Sigma-Aldrich M5895). The eluate was applied to a HiLoad 16/600 Superdex200 pg column (GE Healthcare 28-9893-35 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled in 2x sample buffer for 5 min to elute the proteins and analyzed by SDS-PAGE followed by Western blot using anti c-myc antibody (Sigma-Aldrich, #C3956).
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bioRxiv - Immunology 2020Quote: Frozen tissues were homogenized in 10 volumes (mg : µl) of T-PER tissue protein extraction reagent (Thermo, Cat#78510) containing cocktail inhibitor (Sigma, Cat#P8340) and PMSF ...
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bioRxiv - Immunology 2020Quote: Supernatant from the 293T cells secreting BiTE proteins were collected and subjected to affinity purification using cOmpleteTM His_tag purification resin (Sigma-Aldrich, St.Louis, MO). In some studies ...
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bioRxiv - Microbiology 2019Quote: ... Protein extracts from the cell pellets and culture supernatants were subjected to immunoblotting using α-HA (Sigma-Aldrich, St. Louis, MO, USA) and α-Actin antibodies (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated by SDS-PAGE and visualized by Western blot analysis using primary antibodies for FLAG (Clone M2, Sigma-Aldrich, F1804) and HA (Roche ...
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bioRxiv - Immunology 2020Quote: ... The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G) in PBS ...
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bioRxiv - Immunology 2020Quote: ... Env protein bound beads were further incubated with blocking buffer (PBS [pH 7.4], 0.1% bovine serum albumin [BSA; Sigma], and 0.05% Tween 20) at 37°C to block the unbound sites and the antigenic integrity of Env proteins were assessed by examining their ability to bind to different mAbs by flow cytometry (FACSCanto ...
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bioRxiv - Immunology 2020Quote: ... Total protein in the cell lysate was estimated using the Lowry assay and 30 µg total protein from each cell lysate was used for immunoblotting with anti-HA antibody (Cat# H9658, Sigma, 1:10000), anti-AU1 antibody (Cat# ab3401 ...
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bioRxiv - Genomics 2021Quote: ... Total protein (40 μ g) was electrophoresed on a 10% polyacrylamide gel by SDS-PAGE and transferred to PVDF membranes (Millipore, USA), followed by blocking with 5% milk for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocytosis was assessed by glial fibrillary acidic protein (GFAP) staining using a Cy3-conjugated anti-GFAP antibody (1:200; C 9205, Sigma, Oakville, ON). Brain levels of Bdnf and Htt were determined by western blotting using protein lysates from either whole brain or microdissected brain sections with the following antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... and aliquots of 15 μg protein were separated by SDS-PAGE and blotted onto a polyvinylidene fluoride (PVDF) membrane (Millipore, MA, USA). Membranes were blocked at room temperature for 15 min in QuickBlock™ Western (Beyotime) ...
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bioRxiv - Developmental Biology 2022Quote: Samples were isolated from whole brain as described above and proteins were precipitated from lysis buffer using Trichloroacetic acid (TCA) as follows: 50μl of 100% TCA (Sigma/Millipore – cat# T6399-5G) was added to 200 μl of protein sample at a final concentration of 20% ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed 3 times with 500 µL of lysis buffer and bound protein was eluted with 50 µl of elution buffer (100 µg/mL FLAG peptide (Sigma-Aldrich #F3290), 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry tagged Drosophila Fab1 complexes with anti-mCherry antibody were precipitated by ∼60 µl slurry of washed and blocked protein-G sepharose beads (according to manufacturer’s protocol, Sigma # GE17-0886-01) for 2 hours at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... at 20 °C) and proteins were extracted using lysis buffer (RIPA Thermo Scientific, cOmplete™, EDTA-free Protease Inhibitor Cocktail, Sigma Aldrich). The protein concentration was measured on Tecan (Schoeller ...
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bioRxiv - Immunology 2022Quote: ... 2 x 106 cells/ml were incubated for 24 h with 200 μg/ml ovalbumin protein (OVA, Grade V, Sigma, St.Louis, MO). Prior to being added to cultures ...
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bioRxiv - Genetics 2022Quote: ... The column was calibrated using a gel filtration markers kit for protein molecular weights 29,000-700,000 Da (Sigma-Aldrich, product number MWGF1000).
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked in 5% BSA in TBST and incubated overnight with primary antibodies targeting the following proteins: FLAG (M2; Sigma; 1:1000), phosphorylated tyrosine (P-Y-1000 MultiMab ...
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bioRxiv - Immunology 2022Quote: ... and adhered cells were exposed to exNef or exCont (1 μg/ml protein) for 48 h in the absence or presence of either 20 μM fluvastatin (Sigma, SML 0038) or 10 nM Picropodophyllin (PPP ...
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bioRxiv - Molecular Biology 2022Quote: ... Sixty μl of Dynabeads Protein G were incubated for 1h30 at 4°C under agitation with 16 μg of the anti-Ago2 antibody (Sigma-Aldrich, SAB4200085) in 1X PBS containing 0.1% of Tween 20 ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... see seeding density in transient transfection method) of M-PERTM Mammalian Protein Extraction Reagent plus 40uL 25x cOmpleteTM Protease Inhibitor Cocktail (Sigma-Aldrich 4693132001) and processed according to manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2022Quote: ... Extracts were cleared by centrifugation for 30 min at 12000 × g at 4°C and dilutions of the supernatant were used to quantify protein amounts with Bradford reagent (SIGMA-ALDRICH B6916). Samples were then denatured for 5 min at 95 °C in 4X reducing electrophoresis sample buffer (200 mM Tris pH 6.8 ...
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bioRxiv - Genomics 2022Quote: ... then purified proteins were desalted with PD-10 columns (GE, 17-0851-01) and concentrated using Centrifugal Filter Units (EMD Millipore, UFC803008).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then run on NuPAGE 4-12% Bis-Tris Protein Gels (Thermo #NP0335BOX) and transferred to a PVDF membrane (EMD Millipore #IPVH00005). Blots were probed with primary antibodies overnight at 4°C in 5% blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples were separated on ammediol SDS-PAGE [30] and transferred to a PVDF membrane (polyvinylidene difluoride, EMD Millipore, Burlington, MA, USA) in transfer buffer (10 mM N-cyclohexyl-3-aminopropanesulfonic acid ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skim milk in TBST and target proteins were detected with ANTI-FLAG® M2 antibody (Sigma-Aldrich) and anti-α-tubulin (SantaCruz).
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bioRxiv - Plant Biology 2022Quote: Kinase activity assay was carried out by mixing 2 μg of CORK1KD or GST-CORK1KD-G748E with 3 μg of myelin basic protein (MBP; Sigma-Aldrich, Germany) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: The nucleic acid binding property of protein was further validated using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 μgm recombinant protein was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were then incubated overnight at 4ºC with primary antibodies for myelin basic protein (1:250 Millipore Cat# MAB384-1ML, RRID:AB_240837), neurofilament 160 kD medium (1:250 ...
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bioRxiv - Neuroscience 2022Quote: ... vesicular fraction from brains obtained as described earlier was first pre-cleared for an hour at 4°C with protein A Sepharose beads (Sigma Aldrich-P9424) and then immunoprecipitated for 3 hours at 4°C by agarose beads preincubated with rabbit anti-HTT D7F7 antibody (Cell Signaling ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant that contained the soluble fusion protein was filtered over Millex-GP syringe-driven 0.22-µm PES membranes (Millipore Sigma, Burlington, MD). The fusion protein was purified by fast protein liquid chromatography (FPLC ...
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bioRxiv - Biochemistry 2024Quote: The concentration of both activated proteins—PA-BFP and SumireF—was determined by the bicinchoninic acid assay using the QuantiPro™ Kit (Sigma–Aldrich), following the supplier’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... 20 µL volumes of protein were plated on 35-mm dishes precoated with 1% Pluronic F-127 (Sigma Aldrich, St. Louis, Missouri). Subsequently ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µg proteins were separated by SDS-PAGE (10– 12% gels) and were blotted onto a nitrocellulose membrane (Millipore, Billerica, MA, USA). The membrane was blocked with 5% BSA in TBST at room temperature for 1h ...
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bioRxiv - Microbiology 2024Quote: ... and the immunoprecipitated proteins were eluted with Laemmli buffer and subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma, 1:1,000), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Plant Biology 2024Quote: ... the total proteins in the samples were measured through the BiCinchoninic acid Assay using a kit (Sigma-Aldrich, Saint Louis, MO, USA). The assay was conducted according to the method provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in samples was determined using Bradford assay (Bradford, 1976) against standards of known protein concentration prepared using bovine serum albumin (BSA; Sigma-Aldrich, UK).
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bioRxiv - Microbiology 2024Quote: ... the dried protein pellet was dissolved in 50 µL of 8 M urea (Cytiva, 17131901) in 100 mM NH4HCO3 (ABC; Sigma-Aldrich, 11213) and 5 mM dithiothreitol (DTT ...
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bioRxiv - Physiology 2024Quote: ... For colocalization 1:100 anti-CHRM3 was incubated with 1:500 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07– 623), 1:200 anti-alpha tubulin (Santa Cruz ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 40 μg of each protein sample was reduced with 5 mM tris (2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich, Cat No: C4706) and then diluted in 50 mM Tris HCl ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were harvested and 0.45 µm filtered before loading on to a 1 ml HiTrap™ Protein G HP column (Cytiva) pre-equilibrated in PBS (79382, Sigma-Aldrich). The column was washed with PBS and bound proteins were eluted using 5 ml of 0.1 M glycine pH 2.5 into 1 ml of 1 M Tris pH 8 ...