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Citations for Millipore Sigma :
7251 - 7300 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Uncojugated FITC was separated from the labelled proteins using PD-10 spin columns (Sigma Aldrich, PD Spintrap™ G25, #GE28-9180-04) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... About 20 ug total proteins in each sample were fractionated by 10% SDS PAGE gel and transferred to PVDF membranes (Millipore Corporation, USA) and immunoblotted with primary antibody anti-GFP (Invitrogen-Molecular Probes ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were separated using a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred onto Immobilon-FL PVDF membrane (Millipore). The membranes were blocked in 5% BSA for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation and 400 μg of protein lysate were incubated with 40 μL of Anti-FLAG® M2 affinity gel (Sigma, A2220) for 2 h with end-over-end rotation at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells used for total protein purification were lysed with 2 ml of a buffer containing 1% Sodium dodecyl sulfate (SDS; Sigma-Aldrich #436143), 50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... 15 μg of tumor protein was resolved on 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membrane (Millipore, catalog# IPFL00010). Membranes were blocked with 5% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...
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bioRxiv - Cell Biology 2022Quote: ... the proteins were wet transferred onto ‘Amersham™ Protran™0.2 µm NC’ nitrocellulose membranes (GE10600002 Sigma-Aldrich Handels Gmbh, Vienna, Austria) at constant 80 V for 1,5 h ...
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bioRxiv - Developmental Biology 2022Quote: ... SDS-PAGE and western blot analyses were used to detect proteins and modifications using the following antibodies: anti-phospho SMAD2 (Ser465/467, Sigma, 1:500), Smad2 Polyclonal Antibody (Life Technologies 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Neuroscience 2022Quote: ... The reporter proteins were released from the ER-localized streptavidin ‘hook’ with the addition of 40 μM biotin (Sigma-Aldrich, B4639-100MG) to the coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein from each sample were loaded into 10-20% Tricine sodium dodecyl sulphate– polyacrylamide gel electrophoresis (SDS-PAGE; Sigma-Aldrich, Sweden), followed by immunoblotting on polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: Proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Biochemistry 2022Quote: ... pH 8.1 (final urea concentration < 2 M) before addition of trypsin (1 µg per 50 µg of protein; Sigma-Aldrich Cat# T6567) for a 3 h incubation ...
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bioRxiv - Biophysics 2022Quote: ... about 100 μL supernatant was left and the bound protein was eluted by incubation with 10 μL FLAG peptide (Sigma-Aldrich F3290) at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Refolded CTSL-YLAL-C25S and CTSL-C4S-C25S proteins were concentrated and purified further by gel-filtration chromatography on a Sephacryl S-100 (Sigma-Aldrich, USA) column that had been pre-equilibrated with 20 mM Tris–HCl pH 8.0 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The ratio of protein level or phosphorylation levele against control β-actin protein level (detected with a mouse monoclonal Anti-β-Actin-Peroxidase antibody (A3854 Sigma)) was normalized to untreated wt sample mean.
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bioRxiv - Molecular Biology 2021Quote: ... each sample with equal amount of protein was subjected to SDS-PAGE gel (10% or 15%) and transferred to a PVDF membrane (Millipore, Massachusetts, USA). The membrane was incubated with the specific primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: For phosphorylated proteins RIPA lysis buffer was further supplemented with PIC and 1X phosphatase inhibitor cocktail (Merck Millipore, cat no. 524629-1ML). A cocktail of phosphatase inhibitors (B-Glycerolphosphat (25mM) ...
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bioRxiv - Neuroscience 2021Quote: ... standardized to 1 mg/ml) was precleared with 50 μl Protein G Sepharose 4 Fast Flow (Millipore Sigma Cat#GE17-0618-01) or μ rProtein A Sepharose Fast Flow (Millipore Sigma Cat#GE17-1279-01 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 900 μl of 25% trichloroacetic acid (TCA) before harvesting precipitates on Whatman filter strips (Sigma-Aldrich, Cat: WHA1823035). Filter paper disks were placed in Biofluor scintillation fluid (Perkin Elmer ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal loading was verified in a twin run of electrophoresed individual strips of protein stained with Ponceau S (Sigma, St-Louis, MO) and extracted with PBS supplemented with 1.125 M NaCl and 0.6% Tween-20 (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound dye was removed by 2 washes with 100X excess of protein storage buffer followed by centrifugation in Amicon® Ultra-4 Centrifugal Filter Units (SIGMA MilliPORE). LLPS of dyed and undyed protein was compared as quality control and results were similar (data not shown).
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bioRxiv - Microbiology 2022Quote: ... Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore, cat#UFC805024). The concentrated sample was stored in 50% glycerol at −20°C.
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at >10,000 rpm to remove cellular debris and the supernatant containing extracted protein was transferred to a black flat-bottom microwell plate (Millipore Sigma, M9685). Relative fluorescence intensity of each sample was measured on a SpectraMax iD3 microplate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Systems Biology 2019Quote: ... previously adapted to serum free suspension culture were propagated in a DMEM/F12-based protein free chemically defined medium (PFCDM) supplemented with Soybean peptone (Catalog number P0521, Sigma-Aldrich, USA) and HT supplement (Gibco™ catalog number 11067-030 ...
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bioRxiv - Molecular Biology 2019Quote: ... The Est1:Ku80 interaction was analyzed as above except 4 mg of protein was incubated with 30 μL α-FLAG M2 magnetic beads (Sigma M8823) and samples were analyzed by SDS-PAGE on a 10% gel.
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bioRxiv - Microbiology 2019Quote: ... Relative TLR3 protein expression levels between WT and CRISPR modified cells were obtained by measuring TLR3’s expression against the intracellular β-Actin control protein bound to an anti-β-Actin monoclonal antibody (1:300; Sigma). Protein detection in WES was accomplished according to the manufacturer’s protocol using streptavidin-HRP based methodology (ProteinSimple) ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated using Amicon Ultra-15 centrifugal filter unit with nominal molecular weight limit of 10 kDa (Merck Millipore, Burlington, MA), loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... Thirty to forty-five micrograms of the protein were separated on a 5-12% Tris-acetate gel and electrophoretically transferred to PVDF membranes (Millipore, Billerica, MA). Membranes where then incubated with the primary antibodies against sXbp1 (BioLegend) ...
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bioRxiv - Pathology 2020Quote: ... Proteins were immunoblotted with antibodies against FECH (rabbit, 1:400, LS Bio, #LSC409953, and β-actin (mouse, 1:5000, Sigma-Aldrich, AC40). Secondary antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... protein concentrations in the tissue extracts were measured by using Bicinchorinic Acid Solution / Coppersulfate Solution 50:1 (Sigma-Aldrich, St. Louis, USA). 40 μg of total protein was separated by SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were washed three times with PBS-T at 4°C and the specific proteins were visualized with a chemiluminescence HRP substrate system (Millipore, Massachusetts, USA) using LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Pathology 2021Quote: ... 20 µg of total protein was separated by electrophoresis in a 10% SDS polyacrylamide gel and transferred to a PVDF membrane (Millipore, Billerica, MA) for western blot analysis.
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Neuroscience 2019Quote: ... Equal amount of proteins (20 µg/lane) was separated by SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA). Membranes were blocked for 1 h in 5% nonfat dried milk in either 0.1% Tween20 in PBS (PBST ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was probed against the endogenous proteins by incubation with primary Rabbit anti-myosin VI (1:500, Atlas-Sigma HPA0354863-100UL) or Mouse anti-RNAPII phospho Ser5 (1:500 ...
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bioRxiv - Genomics 2021Quote: ... Eluted proteins were then analyzed by western blotting with anti-U1-70k mouse monoclonal antibody (9C4.1) (05-1588, Merk Millipore, Burlington, MA, USA) at a dilution of 1:500 for the detection of U1-70k ...
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bioRxiv - Biochemistry 2021Quote: ... DSF thermal shift assays were developed to assess protein melting point (Tm) and thermal stability in the presence of putative small molecule ligands: 2-phosphoglycerate (2PG, Sigma-Aldrich 73885), phosphoserine (pSer ...
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bioRxiv - Cell Biology 2021Quote: ... The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore; 10 kDa MWCO). The concentrate was applied to a Superdex 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchange to PBS and concentration of eluted proteins were carried out using Amicon 10 K centrifugal filter units (Millipore, Darmstadt, Germany). Protein quality was analyzed by standard SDS-Page and via the NanoDrop protein concentration was determined.
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bioRxiv - Microbiology 2020Quote: ... Resulting primary cells were first cultured in the selective media to proliferate in the presence of the Rho-associated protein kinase (ROCK) inhibitor (Y-27632, 10 µg/ml, Sigma-Aldrich, Poland) (95) ...
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bioRxiv - Molecular Biology 2021Quote: ... TMG-capped RNAs were isolated from RNase H-treated immunoprecipitates with Protein G Dynabeads coated with an α-TMG antibody (Sigma, MABE302), according to 59.
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bioRxiv - Cell Biology 2021Quote: ... 8% SDS-PAGE was used for protein separation by electrophoresis and transferred to a PVDF membrane (Immobilon®-P, Millipore Corporation, IPVH00010). 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4 ...