Labshake search
Citations for Millipore Sigma :
7351 - 7400 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... malachite green assay (Malachite green oxalate salt, Sigma-Aldrich, cat M6880, Ammonium molybdate tetrahydrate, Sigma, cat 09878) was performed ...
-
bioRxiv - Molecular Biology 2024Quote: ... Hepatic triglycerides were measured using the Triglyceride Quantification colorimetric-/fluorometric assay (MAK266, Sigma-Aldrich, St. Louis, US). A hematoxylin and eosin (HE ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cross-packaging assay was performed in the presence and absence of 10.01 ug herring DNA (Sigma). The herring DNA was included to even out the stoichiometry of DNA-PEI complexes ...
-
bioRxiv - Pathology 2024Quote: ... plurivora growth was investigated via a time-course dual culture assay using cornmeal agar (CMA) (Sigma Aldrich) plates ...
-
bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay following by the manufacturer’s instructions (Sigma). Briefly ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Mitochondrial activity of hAMSC was assessed using the Thiazolyl Blue Tetrazolium Bromide (MTT) colorimetric assay (Sigma-Aldrich) and the Cell Titer-Glo Luminescent Cell Viability assay (Promega ...
-
bioRxiv - Microbiology 2024Quote: Lactate dehydrogenase (LDH) assay was performed with the Cytotoxicity Detection KitPLUS (LDH, Sigma Aldrich, Cat. No. 4744934001) according to manufacturer’s instructions.
-
bioRxiv - Physiology 2024Quote: 5-Bromo-2’-Deoxyuridine (BrdU) incorporation assay was performed in accordance with the manufacturer’s instruction (Millipore, #2752) 18 ...
-
bioRxiv - Biophysics 2024Quote: Assays were performed in a Multiscreen 96-well GF/B microtiter filtration plate (Millipore, cat. no. MSFBN6B) with 210 μL total per well of the following ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined by using the bicinchoninic acid (BCA) assay following the supplier’s protocol (Novagen #712853).
-
bioRxiv - Cell Biology 2024Quote: The EMD MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Pane A – Immunology Multiplex Assay (Merck Millipore) was employed to simultaneously analyze TNF-α ...
-
bioRxiv - Developmental Biology 2020Quote: The cell death detection kit (In Situ Cell Death Detection Kit, Sigma-Aldrich) was used to perform TUNEL analysis according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Trichrome stain (Masson) kit and senescence cells histochemical staining kit (Sigma-Aldrich, Germany); DAB substrate kit (Vector Laboratories ...
-
bioRxiv - Biophysics 2021Quote: ... and then mounted between a semipermeable membrane (Lumox 1lm, Starstedt, Germany) and a coverslip while embedded in Halocarbon 27 oil (Sigma-Aldrich). Live imaging was performed using a Leica SP8 scanning confocal microscope ...
-
bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of cell lysate or purified sEVs were resolved by SDSPAGE and transferred into a polyvinylidene difluoride (PVDF) membrane (Millipore #IPVH00010). After blocking with 5% milk for 1 hour RT ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were separated by SDS-PAGE on a 4-12% Bis Tris gel (NuPage, NP0335BOX) and transferred onto a polyvinylidene fluoride membrane (Millipore, IPVH00010). Membranes were blocked with 5% bovine serum albumin (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were separated on SDS-polyacrylamide gels and transferred either to Immobilon-P or Immobilon-FL PVDF membranes (Millipore, Watford, UK) under semi-dry blotting conditions ...
-
bioRxiv - Cell Biology 2020Quote: ... A total of 30 μg of protein from each sample were subjected to SDS-PAGE electrophoresis and transferred to PVDF membrane (Merck Millipore, USA). Then the membrane was blocked in 5% w/v bovine serum albumin (BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... After blocking the membrane in filtered (Nalgene # 595-4520) TBS-T (50 mM Trisbase, 150 mM NaCl, 0.1 % Tween 20 (Sigma-Aldrich # P1379)) with 3 % bovine serum albumin (BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to an Immobilon polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). The membranes were incubated with primary antibodies overnight at 4⁰C ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were resolved by SDS-PAGE on a 10% polyacrylamide gel and immunoblotted on Immobilon-P transfer membrane (IPVH00010; 21 Millipore Corporation). Membranes were first blocked with TBS containing 0.05% Tween-20 (TBST ...
-
bioRxiv - Molecular Biology 2021Quote: ... Rinsed membranes were incubated in primary antibody solution (primary antibody diluted with 5% bovine serum albumin, BSA, Sigma, 0.1% (w/v) NaN3 in TBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fractions containing AR9 nvRNAP holoenzyme or its mutants were pooled and concentrated (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane, EMD Millipore) to a final concentration of 3 mg/mL ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... equal amounts of total cell lysate (20 μg/lane) were fractionated by SDS-PAGE (10%) and transferred to a PVDF membrane (Millipore, USA) using an iBlot dry blotting system (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... Samples were electrophoresed on 7.5% SDS-polyacrylamide gels and proteins were transferred onto the Immobilon-P membrane (Millipore, New Bedford, MA). Blots were probed with anti-fibrinogen polyclonal antibody (1:100,000 dilution) ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein lysates were electrophoresed on 8% (LRRK2) or 15% (α-synuclein) SDS-PAGE gels and transferred overnight onto PVDF membranes (Millipore). Blots were either blocked with Everyblot blocking reagent (BioRad ...
-
bioRxiv - Molecular Biology 2021Quote: ... was separated on an agarose-formaldehyde gel (Mansour and Pestov, 2013) and transferred to a nylon membrane using the Whatman Nytran SuPerCharge (SPC) TurboBlotter system (Sigma, WHA10416300). After overnight transfer ...
-
bioRxiv - Cell Biology 2022Quote: ... The samples were subsequently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to Immobilon-P polyvinylidene difluoride membranes (IPVH00010; EMD Millipore). Immunoblotting analysis was performed with the indicated antibodies ...
-
bioRxiv - Immunology 2022Quote: ... Total protein lysates (10-20 μg) were separated on 10-15% polyacrylamide gels and transferred onto Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore Sigma). Membranes were blocked in 5% non-fat milk or BSA in PBS with 0.1% Tween 20 (PBST ...
-
bioRxiv - Immunology 2022Quote: ... Samples were resolved using SDS-PAGE and transferred to nitrocellulose membranes before analysis with anti-MYC (Cell Signalling) anti-β-Actin (Sigma) antibodies ...
-
bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
-
bioRxiv - Physiology 2020Quote: ... Proteins were detected after incubation of the membranes with secondary GP anti-rabbit alkaline phosphatase-conjugated antibody (1:30000, cat# A3687, Sigma Aldrich), GP anti-mouse alkaline phosphatase-conjugated antibody (1:20000 ...
-
bioRxiv - Genomics 2020Quote: ... Membranes were blocked at room-temperature in Tris-buffered-saline (TBS) supplemented with 0.1% (v/v) Tween20 (TBST; Sigma Aldrich, P1379) and 5% skim-milk powder (local supermarket ...
-
bioRxiv - Cancer Biology 2020Quote: ... and immune pellets were analyzed by SDS-PAGE electrophoresis using BIORAD Criterion gels and electro-transferred on PVDF membrane (EMD-Millipore IPVH85R). Membranes were incubated overnight at 4°C with primary antibodies and for 2 hours at room temperature with horseradish-conjugated secondary antibodies ...
-
bioRxiv - Microbiology 2021Quote: ... groundwater was pumped directly into a sterile chilled Nalgene filtration unit fitted with a 0.22 µm pore size Isopore polycarbonate membrane (Merck Millipore, Darmstadt, Germany). Approximately 10 L of groundwater was filtered each for metagenomic and metaproteomic sample and 1 L was filtered when samples were taken for 16S rRNA gene amplicon analysis only ...
-
bioRxiv - Neuroscience 2020Quote: ... frozen supernatant was thawed and loaded immediately onto a 10 kDa molecular weight cut-off membrane (Sigma-Aldrich, St. Louis, MO) which was rinsed with 0.1 M NaOH and 50/50 MeOH/H2O ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein from gels were transferred onto PVDF membranes and probed with NSF (SYSY, mouse monoclonal) and beta actin (Sigma, mouse monoclonal) antibodies overnight with shaking ...
-
bioRxiv - Neuroscience 2020Quote: ... as well as secondary anti-mouse and anti-rabbit (Thermo Fischer Scientific) Membranes were incubated with Immobilon® ECL Ultra Western HRP Substrate (Millipore) for 3 minutes prior to image acquisition ...
-
bioRxiv - Bioengineering 2020Quote: ... 15 μL droplets of cell-hydrogel mix were placed on the surface of elastomeric membranes that underwent a silanization process through a nitric acid and (3-Aminopropyl)triethoxysilane (Sigma-Aldrich) treatment ...
-
bioRxiv - Biochemistry 2020Quote: ... samples were resolved under denaturing and reducing conditions using 4-12% Bis-Tris gels (NuPAGE®) and transferred to a PDVF membrane (Immobilon-P, Millipore). Membranes were blocked with 5% nonfat dried milk ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell lysates were separated using electrophoresis on 4-20% SDS-polyacrylamide gels and transferred to PVDF membrane (Millipore, Billerica, MA, USA). For western blot ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein samples were resolved by electrophoresis on 12% SDS–polyacrylamide gels and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corporation) for 20 min at 25 V with the Trans-Blot Turbo (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were blocked at room temperature with either 5% (w/v) non-fat milk or 3% (w/v) BSA (Sigma- Aldrich) in Tris-buffered saline (TBS ...
-
bioRxiv - Neuroscience 2020Quote: ... Tissue was cut into 400 μM thick sections on a tissue chopper and cultured at the medium/air interface on membranes (Millipore PICMORG50) at 37° C in 5% CO2 ...
-
bioRxiv - Neuroscience 2020Quote: ... in retinal samples were resolved by 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto and electro-blotted to nitro-cellulose membranes (Millipore, USA). The membranes were subsequently blocked with 5% nonfat milk for one hour and incubated overnight at 4 °C with primary antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: ... Coring 6.5 mm Transwell with 8.0 um pore polycarbonate membrane insert in 12 well plates were used (Sigma Aldrich, cat# CLS3422). The insert membrane was treated for optimal cell attachment ...
-
bioRxiv - Cell Biology 2021Quote: ... nonfat dry milk in PBS-T for 1 h Membranes were incubated with mouse anti-FLAG M2 antibody (1:500, Sigma-Aldrich) or mouse anti-α-tubulin B512 antibody (1:5000 ...
-
bioRxiv - Cell Biology 2021Quote: ... The proteins were separated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore, USA) followed by blocking with 5% nonfat milk ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 or12% SDS-PAGE gels for approximately 60-70 min in SDS-PAGE running buffer and then transferred to an Immobilon-P polyvinylidene difluoride membrane (Millipore Corp.) using a MiniTransblot apparatus (Bio-Rad) ...
-
bioRxiv - Biochemistry 2020Quote: ... Twenty micrograms of each sample was run on NuPAGE 4–12% Bis-Tris and proteins were electroblotted onto PVDF membranes (Millipore, ISEQ00010), which were further processed as described in the immunoblotting section.