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Citations for Millipore Sigma :
6751 - 6800 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Every other day this medium was replaced with basal medium supplemented with leptin and L-ascorbic acid (Sigma Aldrich) at a concentration of 50 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Five thousand cNF-derived primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated opaque 96-well plates in SCM without IBMX with Forskolin and maintained at 37ºC under a 10% CO2 atmosphere for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates in SCM without IBMX ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were grown on 6-well plates previously coated overnight with a 0.1 mg/ml solution of poly-L-ornithine (Millipore), an important requirement to prevent cell detachment ...
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bioRxiv - Genetics 2023Quote: ... in supplemented RPMI1640 medium (10% heat-inactivated FCS, 2mM L-glutamine, 50IU/ml penicillin, 50μg/ml streptomycin; Sigma-Aldrich), 1×106 MLN cells/well were stimulated by 50ng/ml PMA and 1μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... pregnant dams (E0.7) were given an intraperitoneal injection of anesthetic (working stock: 9 g l-1 Nembutal, Sigma-Aldrich Cat# P37610 ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were plated on 35 mm culture dishes precoated with 0.5 mg/mL poly-L-lysine (Sigma Aldrich, P1274) at a density of 125,000 cells/dish ...
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bioRxiv - Genomics 2023Quote: ... Cells synchronisation in G1/S was performed using 160 µg/ml of L-mimosine for 3h (Sigma Aldrich #M0253).
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bioRxiv - Plant Biology 2023Quote: ... Next, calli were moved to callus regeneration media (CRM; per L: 4.43g of LS, 30g maltose (Sigma/Merck #M5885), 600μl CuSO4 (1mg/ml) ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was discarded and the pellet was resuspended in 200 µL 20% TCA and transferred to 2 mL screwcap tubes containing 0.5 mL 400-600 micron glass beads (Sigma). The tubes were then applied to a Mini-BeadBeater machine (BioSpec Products Inc. ...
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bioRxiv - Cancer Biology 2023Quote: EdU staining was performed on glass coverslips in 24-well plates which were precoated with poly-l-lysine (Sigma) and 5 μg/mL mouse laminin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... the cell suspension enriched in OPCs was centrifuged at 200 × g for 5 min and cells were resuspended in the same medium and plated on 100-mm culture dishes coated with 1.5 μg/ml poly-L-ornithine (Sigma). Two hours after seeding ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were added to wells of a 12-well tray that had been incubated for 30 minutes with 0.01% poly-L-lysine (Sigma) to coat the plastic surface ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells constitutively expressing VHH-EGFP fusions were seeded in Ibidi chambers (9·104 cells per well) coated with poly-L-lysine (mol wt 70,000-150,000) (Sigma Aldrich). They were transiently transfected with 0.25 µg of the expression vectors GSDMD I104N-mCherry or GSDMDNT I104N-mCherry ...
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bioRxiv - Physiology 2023Quote: ... The cells were seeded in 35 mm glass-bottom dishes (Mat-Tek) pre-coated with Poly-L-lysine (Sigma) and transfected with the different constructs one day before imaging ...
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bioRxiv - Immunology 2023Quote: ... Tissue pieces were placed in R10 (RPMI 1640 supplemented with 10% FBS, L-glutamine, and penicillin/streptomycin) with 0.5 mg/ml collagenase VIII (Sigma) and placed in a shaking incubator at 37°C for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs and ESC were cultured in ‘Enhanced medium’ consisting of MES medium supplemented with 0.5µmol/l VPA (P4543-10G, Sigma-Aldrich), 1.5µmol/l CGP77675 (SML0314 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then seeded on 6 mm diameter glass coverslips previously coated with 0.01% poly-L-lysine (Sigma, USA). 24h after seeding ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1mL of 1X PBS: 500µL of cells were fixed and plated on 0.01% poly-L-lysine (Sigma-Aldrich) coated glass-bottom Fluorodishes (WPI ...
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bioRxiv - Plant Biology 2023Quote: Powder resulting of MSC surface abrasion was chemically extracted in the collector tube with extraction buffer (80 μL of 5 mM sodium acetate pH 4.6 containing 1 μL.mL−1 of plant protease inhibitor cocktail (Sigma P9599) per mg of powder) ...
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bioRxiv - Neuroscience 2023Quote: ... at room temperature (22°C) in oxygenated artificial cerebrospinal fluid (aCSF) containing (in mmol/L; all from Sigma, US): 125.99 NaCl (#S9888) ...
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bioRxiv - Plant Biology 2024Quote: ... yeast was grown in selective agar media containing adenine {6.7 g/L yeast nitrogen base without aminoacids (Sigma Y0626), 0.64 g/L complete supplement mixture minus tryptophan (CMS-Trp) ...
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bioRxiv - Developmental Biology 2024Quote: ... consisting of 250 μL of HTF and 1 mM reduced L-glutathione (GSH, Sigma-Aldrich-Merck KGaA, Darmstadt, Germany). To reduce the female to female variability ...
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bioRxiv - Molecular Biology 2024Quote: ... The starter cultures were transferred to 2 L of TB medium supplied with 0.05% antifoam SE-15 (Sigma Aldrich) and 100 µg/ml ampicillin ...
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bioRxiv - Neuroscience 2023Quote: ... The pellets were re-suspended in supplemented medium and plated on poly-L-lysine (0.1 mg/ml, Sigma Aldrich)-coated T-75 flask ...
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bioRxiv - Microbiology 2023Quote: ... Liquid and solid media for Mtb auxotrophic strains were additionally supplemented with 24 μg/mL L-pantothenate (Millipore Sigma), 50 μg/mL L-leucine (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... and then placed on a glass slide previously coated with 0.1 mg/ml of poly-L-lysine (Sigma-Aldrich). Cells were subsequently permeabilized with 0.1% Triton X-100 for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 1.5 μL of the diluted culture was added onto a dry Hypho minimal agarose pad (W: 0.4 cm × L: 0.4 cm × H: 0.1 cm) with 1% (m/v) agarose (Sigma Aldrich, MO), and dried at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Culture medium was replaced with 100 µL medium containing 5 mg/mL filtered 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (#M5655, Sigma-Aldrich) and incubated for 2 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... and then transferred to maturation media (DMEM containing low glucose (2 g/L) with Palmitic acid (200 uM, Sigma), Dexamethasone (100 ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden hydrogels were cut into smaller pieces and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: SLI-WT parasites were allowed to settle onto 12 mm coverslips pre-treated with 10% Poly-L-Lysine (Sigma) in Milli-Q water ...
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bioRxiv - Microbiology 2023Quote: ... It is a complex medium containing 2.5 g/l partially purified mucin from porcine stomach (Type III, Sigma M1778), 5 g/l trypticase peptone (Roth 2363.1) ...
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bioRxiv - Bioengineering 2024Quote: ... the calcium signal was stimulated with a micropipette containing 10 μmol/L 5’-ATP-Na2 solutions (Sigma-Aldrich, A1852)51.
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bioRxiv - Microbiology 2023Quote: ... approximately 8 L of seawater was filtered onto 0.22-μm Sterivex-GP pressure filter units (Millipore, Billerica, MA, USA). The filters were immediately soaked in 2 mL of RNAlater Stabilization Solution (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Sterility was qualitatively measured of initiations by sample culture indexing in autoclaved L&W Nutrient Broth (Millipore, MA, USA): Concentrations of 45.22g/L were prepared as instructed on bottle ...
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bioRxiv - Microbiology 2024Quote: ... they were diluted 1:100 in fresh 10 ml of the specified growth medium supplemented with 0.2% (w/v) L-arabinose (Sigma) and 1 mM glycerol (Sigma ...
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bioRxiv - Microbiology 2024Quote: For field emission scanning electron microscopy glass coverslips were coated with a poly-L-lysine solution (Sigma, Munich, Germany), 30 µl of the samples were added ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden were cut into 6x6 mm squares and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... Germany) and sodium bicarbonate (0.08 g/l) supplemented with Guillard’s F/2 Marine Water enrichment solution (Sigma-Aldrich, Belgium) and an antibiotic mixture (500 mg/l penicillin ...
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bioRxiv - Plant Biology 2024Quote: ... on Petri dishes containing KNOP medium (pH 5.8) (Waller et al., 2023) solidified with 7g/L Gelrite (Gelzan CM, G1910 Sigma). We maintained the plants by vegetative propagation (e.g. ...
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bioRxiv - Neuroscience 2024Quote: The surface of each cover slip was coated with 0.5 mg/ml poly-L-lysine diluted in boric acid buXer (Sigma). Embryos were harvested from pregnant ...
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bioRxiv - Cancer Biology 2024Quote: ... Human astrocytes (Gift from the laboratory of Anders Nykjær, Aarhus University, Aarhus, Denmark) were cultured on poly-l-lysine (Sigma)-coated dishes and kept in astrocyte basal medium (ScienCell ...
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bioRxiv - Microbiology 2024Quote: ... Brain Heart Infusion Agar (BHI, Biokar Diagnostics) supplemented with 50 mg/L amphotericin (Sigma Aldrich, Saint-Louis, MO, USA) was used to count total aerobic bacteria after 48 h of incubation at 28°C ...
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bioRxiv - Genomics 2024Quote: ... 12-well plates for differentiation were coated with 15 ug/mL Poly-L-Ornithine (Sigma-Aldrich; Cat. No. P3655) in DPBS ...
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bioRxiv - Biochemistry 2024Quote: ... Proteoliposomes (100 μL) were loaded into the wells of a MultiScreenHTS + HA 96-well filter plate (pore size 0.45-μm, Millipore). Uptake of the radiolabelled substrate was initiated by the addition of 100 μL buffer containing 2.5 μM [14C]-malate (American Radiolabelled Chemicals) ...
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bioRxiv - Physiology 2024Quote: ... The final pellet has been resuspended in cold 4.5g/L glucose 1xDMEM supplemented with 0.2% bovine serum albumin (BSA, Sigma-Aldrich). The muscle cell suspension was used either to sort MuSCs by fluorescence-activated cell sorting (FACS)-sorting or directly seeded on Matrigel coated dishes ...
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bioRxiv - Neuroscience 2024Quote: ... and seeded at a density of 2.0 x 104 cells/cm2 on glass coverslips coated with Laminin/Poly-L-Ornithine (Sigma and Merck-Millipore), or at a density of 5.0 x 104 cells/cm2 on Laminin-/Poly-L-Ornithine coated plates in StemDiff Forebrain Neuron Maturation medium (Stem Cell Technologies ...
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bioRxiv - Biophysics 2024Quote: Cells were cultured to reach 70% confluency before being treated with 10 µM s-trityl-L-cysteine (Sigma-Aldrich) overnight to block them in prometaphase ...