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Citations for Millipore Sigma :
6651 - 6700 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... drinking water of dams in the Cd-exposed group was supplemented with a final concentration of 0.5 μg/L (500 ppb) cadmium chloride (CdCl2; CAS 10108-64-2; 99.99% purity, Lot MKBM1769V, Sigma Aldrich). Beginning on PND10 ...
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bioRxiv - Biophysics 2021Quote: ... and filter sterilized 0.1 mg/ml poly-L-lysine hydrobromide (P1274, MW 70,000-150,000, Sigma-Aldrich, St. Louis MO) in PBS were prepared prior to unroofing ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated in serum-free RPMI 1640 medium supplemented with cytochalasin B (10 µmol L-1; Sigma, Switzerland) for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... and expanded to 70-85% confluency in DMEM Glutamax (+ 4.5g/L D-Glucose, - Pyruvate) supplemented with 10% FBS (Sigma), 1% G418 (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Bladders were pre-treated by transurethral instillation of 25 μL poly-L-lysine (0.1mg/ml in PBS (Sigma-Aldrich)) for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: The amounts of remaining HRP conjugates after 3 washes with the buffer were measured with the addition of 100 μl of 50 mM phosphate-citrate buffer (pH 5.0) containing 0.4 μg of o-phenylenediamine dihydrochloride (OPD) (Sigma-Aldrich) to develop at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... 70 L of groundwater were filtered onto a 0.1 μm pore-size PTFE membrane filter (Merck Millipore, Darmstadt, Germany). The flow-through was collected in a sterilized container ...
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bioRxiv - Microbiology 2020Quote: ... Randomly chosen fish were sacrificed by an overdose of filtered tricaine methane sulfonate solution (tricaine, Sigma, 300 mg/L). Whole fish were mechanically disrupted in Lysing Matrix tubes containing 1 ml of sterile water and 425-600 µm glass beads (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... the γ-secretase inhibitor (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester (DAPT; R&D) or dimethyl sulfoxide (DMSO; Sigma) was added to media at the concentrations indicated.
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bioRxiv - Immunology 2021Quote: 7-8 week-old B6J females were treated for 10 consecutive days with ampicillin (1 g/L; A0166 Sigma), vancomycin (500 mg/L ...
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bioRxiv - Microbiology 2021Quote: ... Fluids from a hydrothermal plume above Anemone and background seawater were collected using a Seabird SBE911 CTD and 10 L Niskin bottles and 3 L of the plume and seawater fluid was filtered through 0.22 μm Sterivex filters (Millipore). DNA was extracted from the filters using a phenol-chloroform method adapted from Crump et al ...
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bioRxiv - Neuroscience 2021Quote: ... while mouse primary astrocytes (~3.5 × 105 cells) were seeded on poly-L-lysine coated transwell filters (3450; Sigma-Aldrich) and maintained in astrocyte-media ...
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bioRxiv - Genetics 2020Quote: ... The obtained mix of double-stranded DNA oligonucleotides was methylated by murine DNMT3A or DNMT3B catalytic domain for 60 min at 37 °C in the presence of 0.8 mM S-adenosyl-L-methionine (Sigma) in reaction buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... bottles of 0.75-1.5 L TB supplemented with appropriate antibiotics and 100-200 μL of antifoam 204 (Sigma-Aldrich) were inoculated with the overnight cultures ...
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bioRxiv - Neuroscience 2021Quote: ... animals received daily intraperitoneal (I.P) injections of L3,4-dihydroxyphenylalanine methyl (L-DOPA, 3 day at 3mg/kg, then 6mg/kg, Sigma-Aldrich) and the peripheral DOPA decarboxylase inhibitor bensezaride hydrochloride (12mg/kg ...
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bioRxiv - Microbiology 2022Quote: ... The MisKO pre and batch cultures were conducted in YPD with 5 mmol L-1 hypoxanthine (Sigma-Aldrich GmbH) and 100 mg L-1 nourseothricin ...
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bioRxiv - Cell Biology 2022Quote: ... the following medium was also used instead of the imaging medium: 30 mM HEPES pH 7.4 containing 9.3 g/l Minimum Essential Medium Eagle (Sigma Aldrich), 10% FCS ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of a 0.2 mg/ml GO suspension (from a 2 mg/ml stock, diluted in deionized water (SIGMA) was applied onto the glow-discharged grids ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... 10 µL of the HDX reaction were removed and quenched by diluting 1:6 into 0.75% formic acid (FA, Sigma), and digested for two min with porcine pepsin (0.25 mg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... and then transferred to maturation media [DMEM containing low glucose (2□g/L) with Palmitic acid (200□uM, Sigma), Dexamethasone (100□ng/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... the supernatant was replaced by 400µL of primary antibody diluted to 1:200 in Wash Buffer complemented with 2mM EDTA and 0.02% Digitonin (Millipore). Cells were incubated for one hour at RT ...
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bioRxiv - Genetics 2022Quote: ... The embryos were washed through a series of PBS + 0.4% BSA prior to mounting onto Poly-L-Lysine (Sigma) coated (0.01% in H2O ...
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bioRxiv - Plant Biology 2022Quote: ... Next, calli were moved to callus regeneration media (CRM; per L: 4.43g of LS, 30g maltose (Sigma/Merck #M5885), 600μl CuSO4 (1mg/ml) ...
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bioRxiv - Physiology 2022Quote: ... followed by two weeks with water supplemented with T3 (0.5 mg/L, Sigma-Aldrich T6397, in 0.01 % of BSA). Control animals received only 0.01 % BSA in the drinking water over the whole treatment period ...
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bioRxiv - Microbiology 2022Quote: ... then 50 µL of each supernatant was added to 400 µL of Salkowski’s reagent (2.0 mL of 0.5 M FeCl3 + 98.0 mL of 35% HClO4) (Sigma-Aldrich, Darmstadt, Germany). The contents were allowed to stand for 20 min for color development ...
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bioRxiv - Microbiology 2022Quote: ... Fixed bacteria were washed with PBS and a 5 μl suspension was then centrifuged onto coverslips pre-coated with 0.01% (w/v) poly-L-lysine (Sigma) in a 24-well tray (16,000 ×g ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 20 μg of each protein lysate in 10 μl was digested with increasing amounts of chymotrypsin (0.05 μg/ml to 0. 2 μg/ml, final volume, Sigma) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: We obtained thirtysix 20 amino acid peptides overlapping by 5 amino acids in 0.5mg amounts in 96 well format microtubes based on the entire human NF-L sequence in NP_006149.2 (Sigma-Aldrich). Peptides were diluted in 50% acetonitrile/50% PBS to a concentration of 2.5mg/mL and 2mL of each peptide was arrayed in a 96 well plate ...
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bioRxiv - Neuroscience 2022Quote: ... Each tissue punch was resuspended in NIM-DP-L (1X NIM-DP, 0.1% Triton X-100 (Sigma, T8787-100ML)) buffer before being transferred to the dounce homogenizer ...
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bioRxiv - Molecular Biology 2023Quote: ... Permeabilized cells were subsequently washed three times with PBS and blocked with PBS+ (5 g/L BSA (Sigma Aldrich) and 1.5 g/L glycine (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... brains were mounted onto the stage with superglue and then sectioned at 400 μm thick on a Leica VT1000S vibratome in ice-cold dissection media (95.2 g/L Hank’s Balanced salt (Sigma H2387), 4.2 mM NaHCO3 ...
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bioRxiv - Plant Biology 2023Quote: ... 5.5 g L-1 agar-agar (Dinâmica) was added and the culture was supplemented with picloram or its analogs triclopyr (Sigma) and clopyralid (Sigma ...
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bioRxiv - Physiology 2022Quote: ... 80 μg of protein were incubated in 10 mM L-cysteine and 1 mM pyridoxal 5′-phosphate hydrate (Sigma); or ...
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bioRxiv - Microbiology 2022Quote: ... 1.5 L of water was filtered through a 0.22 μm polyethersulfone filter in a Sterivex filter unit (Millipore Sigma) and stored in a −20°C freezer ...
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bioRxiv - Plant Biology 2022Quote: Individual isolates were cultured on a 1/10 dilution of tryptic soy broth (TSB, Difco) agar (1.5%, m/v) media containing 50 mg/L thiabendazole (Sigma) and incubated for 48 hours at 26°C ...
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bioRxiv - Microbiology 2022Quote: ... pneumophila strains were cultured at 37°C in BYEα broth or BCYEα agar plates (67) containing 0.4 mg/ml L - cysteine sulfate (Sigma), 0.135 mg/ml ferric nitrate (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... and adhered to 12 mm diameter round glass coverslips (Paul Marienfeld GmbH & Co) previously coated with 0.01% poly-L-lysine (Sigma) for 20 min ...
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bioRxiv - Biophysics 2022Quote: Human embryonic kidney cell line (HEK-293T) were seeded on 25 mm No.1.5 H cover glass (Marienfeld) coated with poly-L-lysine (Sigma). 0.15 million cells were maintained in 2ml Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Bioengineering 2022Quote: ... A standard curve was prepared using 20 mM lactate stock solution by adding sodium L-lactate (L7022, Sigma-Aldrich) to dH2O ...
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bioRxiv - Cancer Biology 2022Quote: ... The aqueous mobile phase A consisted of 95:5 (v:v) water:acetonitrile supplemented with 20 mmol/L ammonium acetate and ammonium hydroxide (Sigma-Aldrich). Mobile phase B was 100% acetonitrile ...
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bioRxiv - Immunology 2023Quote: ... 50 μL of 12,5% NTA in DOPC large unilamellar vesicles were diluted in PBS-0.2% BSA (BSA from Sigma Aldrich) were added to ultraclean glass coverslips (Schott ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were first slightly anesthetized with a bath in tricaine (160 mg/L in fish water; A5040, Sigma-Aldrich) to reduce movements ...
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bioRxiv - Neuroscience 2024Quote: ... cortical neurons were plated at a density of 300,000 on 6-well plates coated with a solution of 0.0005% poly-l-ornithine and laminin (2.5 μg/ml) (Sigma Aldrich). Neurons were kept in supplemented Neurobasal at 37°C and 5% CO2 for 14-16 days (DIV 14-16).
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bioRxiv - Microbiology 2024Quote: ... they were diluted 1:100 in fresh 10 ml of the specified growth medium supplemented with 0.2% (w/v) L-arabinose (Sigma) and 1 mM glycerol (Sigma ...
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bioRxiv - Microbiology 2024Quote: For field emission scanning electron microscopy glass coverslips were coated with a poly-L-lysine solution (Sigma, Munich, Germany), 30 µl of the samples were added ...
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bioRxiv - Developmental Biology 2024Quote: ... consisting of 250 μL of HTF and 1 mM reduced L-glutathione (GSH, Sigma-Aldrich-Merck KGaA, Darmstadt, Germany). To reduce the female to female variability ...
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bioRxiv - Molecular Biology 2024Quote: ... The starter cultures were transferred to 2 L of TB medium supplied with 0.05% antifoam SE-15 (Sigma Aldrich) and 100 µg/ml ampicillin ...
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bioRxiv - Plant Biology 2024Quote: ... yeast was grown in selective agar media containing adenine {6.7 g/L yeast nitrogen base without aminoacids (Sigma Y0626), 0.64 g/L complete supplement mixture minus tryptophan (CMS-Trp) ...
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bioRxiv - Plant Biology 2024Quote: ... Sterility was qualitatively measured of initiations by sample culture indexing in autoclaved L&W Nutrient Broth (Millipore, MA, USA): Concentrations of 45.22g/L were prepared as instructed on bottle ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were plated in 12-well plates on 18mM glass coverslips coated with poly-L-lysin-hydrochloride (Sigma- Aldrich), in a plating medium (500mL MEM ...