Labshake search
Citations for Millipore Sigma :
6401 - 6450 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ATPase activity of Wt and mutant HelR proteins was determined using the colorimetric ATPase assay kit (Sigma). The ATP hydrolysis reaction was performed by incubating 0.3 µM HelR with 1 mM ATP (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... The amount of NADH and NAD+ in samples was quantitated using the NAD/NADH quantitation kit (Sigma). Briefly ...
-
bioRxiv - Plant Biology 2021Quote: ... 50 µL of the supernatant were taken for iron measurements using the Iron Assay Kit (Sigma, USA) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cytochrome c release was measured by flow cytometry using the Cytochrome c Release Apoptosis Assay Kit (Millipore).
-
bioRxiv - Developmental Biology 2022Quote: RNA was extracted from either cells or tissues using GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... BCA was performed on the freshly isolated proteins using a Pierce BCA protein assay kit (Sigma, 23227) to determine the protein concentration ...
-
bioRxiv - Cancer Biology 2022Quote: The RIP assay was performed using a Magna RIPTM RNA Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... a 100ml culture was harvested for DNA extraction using GeneElute –Mammalian Genomic DNA Miniprep Kit (Sigma- Aldrich). PCRs were done on different genomic regions flanking an IES.
-
bioRxiv - Synthetic Biology 2022Quote: ... and analysed using the WesternBreeze Chromogenic anti-mouse Kit with a primary anti-FLAG (F4042, Sigma-Aldrich) antibody at a 4000x dilution ...
-
bioRxiv - Synthetic Biology 2022Quote: Total bile acids in feces was measured using Bile Acid Assay Kit (Sigma-Aldrich MAK309, Merck, France). The L-lactate concentration was measured using a L-lactate Assay Kit (Sigma-Aldrich MAK329 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The L-lactate concentration was measured using a L-lactate Assay Kit (Sigma-Aldrich MAK329, Merck, France). 20 μL of pre-treated and 0.4 μm filtered feces samples were used for each reaction ...
-
bioRxiv - Microbiology 2022Quote: ... genomic DNA was prepared using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, St. Louis, Missouri, USA) according to the manufacturer’s guidelines and sequenced at the Microbial Genome Sequencing Center (MiGS ...
-
bioRxiv - Immunology 2022Quote: ... For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma) in accordance with manufacturers recommendations.
-
bioRxiv - Developmental Biology 2022Quote: Nicotinamide adenine dinucleotide (NAD) concentration was measured using the NAD Quantitation kit (MAK037, Sigma-Aldrich, MO, USA). In brief ...
-
bioRxiv - Microbiology 2022Quote: Total RNA was isolated from brains using the Sigma GenElute Total Mammalian RNA Miniprep Kit (Sigma, RTN350), according to the manufacturer protocol with the addition of a Sigma DNase digest reagent (Sigma ...
-
bioRxiv - Plant Biology 2022Quote: ... chloroplasts were isolated from fresh leaf tissue using a commercially available chloroplast isolation kit (Sigma Product #: CPISO) following manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... RNA was extracted from either cells or tissues using GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2021Quote: ... The BDNF of offspring hippocampus was measured using the ChemiKineTM BDNF Sandwich ELISA kit (CYT306; Chemikine, Millipore). A total of 80 µg/well of hippocampus protein was used and samples were analysed in duplicates ...
-
bioRxiv - Cell Biology 2021Quote: Duolink In Situ Red Starter Kit Mouse/Rabbit Proximity Ligation Assay was purchased from Millipore Sigma (DUO92101) and was performed according to the manufactures protocol using KLK10 and HTRA1 antibodies described above.
-
bioRxiv - Molecular Biology 2020Quote: ... staining en bloc with uranyl acetate at the 30% stage before embedding (Epoxy embedding medium kit – Sigma). 50 nm ultrathin sections were cut on a Leica UC6 ultramicrotome and imaged on an FEI 120kV Spirit Biotwin TEM with a F4.15 Tietz camera.
-
bioRxiv - Biophysics 2020Quote: The RBD and spike proteins were chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). For the RBD ...
-
bioRxiv - Microbiology 2021Quote: ... Glucose concentration of each supernatant sample was analysed using the Glucose (GO) assay kit (GAGO-20; Sigma) in a 96-well format ...
-
bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
-
bioRxiv - Cell Biology 2021Quote: The cytoskeletal fraction was separated using the ProteoExtract® Cytoskeleton Enrichment and Isolation Kit (17-10195; Millipore), following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: RIP assay was performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma, Burlington, MA) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: For the annexin V/propidium iodide assay (using a staining kit from Sigma−Aldrich, cat. no. 11858777001), mouse cortical neurons were treated at DIV 12 with either 500 μM sulfo NONOate or 500 μM spermine NONOate for 2.5 h at 37 °C and 5% CO2 ...
-
bioRxiv - Systems Biology 2022Quote: ... The dried pellet was used for protein analysis using Bicinchoninic Acid Kit for Protein Determination (Sigma Aldrich).
-
bioRxiv - Cancer Biology 2022Quote: ChIP experiments were performed using EZ-Magna ChIPTM A/G Chromatin Immunoprecipitation Kit (Merck Millipore, 17-10086) according to the manufacturer’s procedures ...
-
bioRxiv - Cell Biology 2022Quote: Hydroxyproline content was determined according to the manufacturer’s instructions (Hydroxyproline Assay Kit, Sigma-Aldrich, St. Louis, MO). Briefly ...
-
bioRxiv - Neuroscience 2019Quote: ... The specific steps were carried out according to the instructions of a TUNEL kit (Merck Millipore, USA). Five regions were randomly selected from the ischemic penumbra of each brain slice under a 400× magnification objective lens (Olympus ...
-
bioRxiv - Plant Biology 2019Quote: DNA isolations were performed using the GenElute™ Plant Genomic Miniprep Kit (Sigma-Aldrich, St. Louis, MO). DNA quality and quantification were determined using a Nanodrop ND-1000 (Nanodrop Technologies ...
-
bioRxiv - Developmental Biology 2019Quote: ... harvested to prepare nuclear extract using the CelLytic™ NuCLEAR™ Extraction Kit (Sigma, Missouri, Unites States). For luciferase reporter assays ...
-
bioRxiv - Systems Biology 2019Quote: ... Nuclei were washed once with 15 ml Nuclear Isolation Buffer from the CelLytic PN kit (Sigma-Aldrich) and re-pelleted ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The medium alanine aminotransferase (ALT) activity was quantified using the ALT assay kit (MAK052, Sigma-Aldrich Corp.).
-
bioRxiv - Pathology 2019Quote: ... Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by using the spectrometric kits (Sigma Diagnostics). Triglyceride ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... DHFR activity and screening of DHFR inhibitors testing was examined using the Dihydrofolate Reductase Assay Kit (Sigma). The assay is based on the ability of DHFR to catalyze the reversible nicotinamide adenine dinucleotide phosphate (NADPH ...
-
bioRxiv - Cell Biology 2019Quote: ... The proximity ligation assay was conducted using a DuoLink In Situ Mouse/Rabbit PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... The CRP concentration in mouse serum was measured using the mouse CRP ELISA kit (Sigma-Aldrich, RAB1121) according to the manufacturer’s protocol.
-
bioRxiv - Plant Biology 2019Quote: ... Total RNA was extracted from the collected samples using Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) and was treated with RNase-free DNaseI (Qiagen ...
-
bioRxiv - Cancer Biology 2019Quote: ... Plasma concentration of non-sulfated bile acids were measured with the Bile Acid Assay Kit (Sigma-Aldrich) using a Fluoroskan Ascent fluorescence multiwell plate reader (Thermo Labsystems) ...
-
bioRxiv - Pathology 2020Quote: ... Serum Triglyceride and serum free glycerol were quantified using Serum Triglyceride Determination Kit (Sigma-Aldrich, cat# TR0100) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... All constructs were prepared for transfection using the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich).
-
bioRxiv - Synthetic Biology 2020Quote: ... Residual concentrations of formate were checked by a Formate Assay Kit following the manufacturer’s protocol (Sigma-Aldrich).
-
bioRxiv - Bioengineering 2020Quote: ... Multiplexed chemokine and cytokine analysis was performed using Milliplex MAP Rat Cytokine/Chemokine Magnetic kit (Millipore Sigma) and analyzed using a MAGPIX Luminex instrument (Luminex) ...
-
bioRxiv - Plant Biology 2021Quote: ... RNA from 100 mg of leaf tissue was extracted using the Spectrum Total RNA kit (Sigma-Aldrich). An on-column DNase digestion (Sigma-Aldrich ...
-
bioRxiv - Physiology 2021Quote: ... sTRAP assay was measured with acetate buffer from acid phosphatase leukocyte kit (Sigma-Aldrich, St. Louis, MO), 1M sodium L-tartrate dibasic dihydrate (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... following incubation using materials from the PKH26 Red Fluorescent Cell Linker Kit (Millipore Sigma, St. Louis, MO), the Exos were washed three times with PBS before ultracentrifugation at 100,000□x□g for 20□min at 4□°C using Beckman L7-80 and rotor SW41 (Beckman Coulter ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations of wholecell lysates were determined by the QuantiPro™ BCA Assay Kit (Sigma-Aldrich, QPBCA). The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore ...
-
bioRxiv - Plant Biology 2021Quote: ... the chloroplasts were first isolated from the intact leaves using a chloroplast isolation kit (Sigma-Aldrich, USA), after which further intact chloroplasts were harvested using a 40/80% Percoll gradient ...
-
bioRxiv - Microbiology 2021Quote: Cell proliferation activity was measured by BrdU incorporation assay using a Cell Proliferation ELISA kit (Sigma-Aldrich). The wells of 96-well plates were seeded with 5000 HSC-2 or HO-1-N-1 cells ...