Labshake search
Citations for Millipore Sigma :
6451 - 6500 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: Proximity ligation assay was performed using the Duolink® In Situ Orange Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmids were extracted and purified using the GenElute plasmid miniprep kit (Sigma-Aldrich, St. Louis, MO, USA). EcoRI and KpnI cut sites were introduced using restriction enzyme cut-site primers (Supplementary file 3 ...
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bioRxiv - Cell Biology 2021Quote: The glycerol content of cell culture media was quantified using a Glycerol Assay Kit (Sigma Aldrich MAK117) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Protein-protein interactions were analyzed using Duolink in situ orange starter fluorescence kit (mouse/rabbit, Sigma-Aldrich) in human osteosarcoma U2OS cells (WT ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested backbone was purified from 0.7 % Agarose Gel using the GenEluteTM Gel extraction Kit (Sigma Aldrich, NA1111). Vector and miRNA target inserts were ligated at 1:10 molar ratio using 400 U of T4 DNA Ligase (NEB ...
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bioRxiv - Genetics 2020Quote: ... nuclei were enriched from the Dounce-homogenized brain tissues using Nuclei EZ prep Kit (Sigma, NUC-101). DNA were digested with micrococcal nuclease (MNase ...
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bioRxiv - Neuroscience 2021Quote: Glutamate quantity in hippocampal homogenates (10 μl) was measured colorimetrically utilizing Glutamate Assay Kit (Sigma-Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... paraffin sections were dewaxed and rehydrated and stained using an Elastic Stain Kit (Millipore, HT25A, Burlington, MA). Brightfield imaging was done on a Nikon E600 microscope with Nikon NIS-Elements software ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription of digoxigenin-labeled probes was performed using the DIG RNA Labeling Kit (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was extracted using the GenElute mammalian total RNA miniprep kit from Sigma (Sigma, RTN350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation was performed using manufacturer’s instructions for the EZ-Nuclear RIP Kit (EMD Millipore, #17-10 523), followed by cDNA synthesis and qRT-PCR for 5’ETS ...
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bioRxiv - Evolutionary Biology 2020Quote: All Digoxygenin (DIG) labeled probes were generated using the Roche DIG labeling kit (Sigma Cat no 11277073910). Antisense probes were generated using Pme1 (NEB cat no R0560S ...
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bioRxiv - Cell Biology 2020Quote: ... were constructed by the ligation-independent cloning method using a pET-41 Ek/LIC vector kit (Novagen). The coding sequences of human LC3B and GATE-16 proteins were amplified by PCR with KOD-Plus-Neo DNA polymerase (Toyobo) ...
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bioRxiv - Genetics 2021Quote: ... leptin and adiponectin were measured in plasma from fasted mice using ELISA kits from Millipore (EZRMI-13K), Mercodia (10-1281-01) ...
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bioRxiv - Immunology 2020Quote: ChIP assays were performed with an EZ ChIP kit purchased from Upstate Biotechnology (Millipore Corporation Billerica, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: Deglycosylation of Bl-Eng2 was performed by using the GlycoProfile(tm) IV Chemical Deglycosylation kit (Sigma-Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... to be further randomly amplified using a modified Whole Transcriptome Amplification 2 (WTA2) kit procedure (Sigma-Aldrich). The products were purified ...
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bioRxiv - Microbiology 2020Quote: ... total protein concentration was measured in triplicates by Total Protein kit (Sigma-Aldrich, St. Luis, MO, US) using bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... paraffin-embedded tissue sections (3-mm) using commercial Masson’s trichrome staining kits (Sigma–Aldrich, St. Louis, MO) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The amplified hoc products were gel-extracted following manufacturer’s protocol (GenElute™ Gel Extraction Kit, Sigma Aldrich). Wildtype and D246N hoc genes were individually cloned in-frame to expression vector pPROEX-HTb ...
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bioRxiv - Immunology 2021Quote: ... Nuclear isolation was performed using Nuclei EZ Prep kit per manufacturer’s instructions (Sigma Aldrich, St. Louis, MO). Fractions were subsequently lysed with Triton X-100 lysis buffer (1% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA was extracted from clonal cell lines using the GenElute Mammalian Genomic DNA kit (Sigma-Aldrich) and the K6a and K6b gene fractions where the indel mutations were expected were amplified by PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with primary antibodies (1:200) which were diluted with Immunoreaction Enhancer Kit (Millipore sigma) overnight at 4°C in a humid chamber ...
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bioRxiv - Neuroscience 2022Quote: ... DNA for experiments was prepared using either the GenElute HP Endotoxin-Free Maxi Prep Kit (Sigma, NA0410) or the EndoFree Plasmid Maxi Kit (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... genomic DNA from EpiLC at D3 was isolated using the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma) with RNase treatment ...
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bioRxiv - Cell Biology 2022Quote: The extraction of RNA from MEF was performed with the GenElute Mammalian Total RNA Miniprep Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The subcellular fractionation was conducted according to the instruction of nucleocytoplasmic separation kit ( Sigma, N-3408-200ML). The nucleocytoplasmic fractions were then determined by Western blotting with the relevant antibodies.
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bioRxiv - Immunology 2022Quote: ... with protein concentration determined by Coomassie G-250 Bradford protein assay kit following manufacturer’s instructions (Sigma, UK).
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from banana organs using a Spectrum Plant Total RNA Kit (Sigma Aldrich, India) and treated with RNAse-free DNase I (Fermentas Life Sciences) ...
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bioRxiv - Plant Biology 2022Quote: ... Biotinylated DNA fragments were purified following the NucleoSpin Gel clean-up kit (Sigma-Aldrich, catalog No. 740609.50) protocol ...
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bioRxiv - Neuroscience 2023Quote: ... ethanol concentrations were measured using the NAD-ADH Reagent kit following the manufacturer’s protocol (Sigma-Aldrich, N7160). The 340 nm spectrophotometric absorbance values were converted to mM and adjusted by the estimated 1 μL volume of an average fly to calculate ethanol concentrations.
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bioRxiv - Plant Biology 2022Quote: ... and fruit tissues were isolated using the SpectrumTM Plant total RNA kit (Sigma, USA, cat. no. STRN50). Library preparation (Illumina Stranded mRNA Prep Ligation Kit ...
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... A horseradish peroxidase-coupled secondary antibody was used and revealed with the Immobilon-P kit from Millipore.
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bioRxiv - Neuroscience 2024Quote: ... Proximity ligation assays were performed on fixed cultured OPs using the Duolink® PLA kit (Sigma-Aldrich). This was conducted using anti-INO80 and anti-OLIG2 antibodies along with anti-SOX10 (positive control ...
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bioRxiv - Microbiology 2024Quote: ... 5 and 7 dpi were quantified using the Milliplex MAP Porcine Cytokine/ Chemokine Magnetic bead kit (Millipore) and the BioPlex Magpix Reader (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Neuronal cytotoxicity was determined with the Lactate Dehydrogenase Activity Assay Kit according to the manufacturer’s instructions (Sigma). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... Nuclei extraction and immunoprecipitation were conducted as previously described33 using CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich) and high-affinity GFP-Trap magnetic agarose (ChromoTek ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA extraction from inter-pathogen zone was carried out using an RNAeasy kit from Sigma Aldrich, and the RNA quality was evaluated using a Nanodrop spectrophotometer ND1000 ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from bud samples using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) in accordance with the manufacturer’s protocol and treated with genomic DNAse using DNASE 70-On Column DNase I Digestion set (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: The MTT assay for cell proliferation was performed using MTT Cell Growth Assay Kit (Sigma Aldrich CT02). HEK293 cells transfected with either cmv-gfp (control ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated with the Spectrum Plant Total RNA Kit (Sigma-Aldrich, Saint-Louis, MO, USA) using protocol B and treated with DNase I (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed as described by the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Darmstadt, Germany) using the kit’s columns and reagents following the protocol for Gram-positive bacteria for all samples ...
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bioRxiv - Neuroscience 2024Quote: ... AAV purity was tested by silver staining using the ProteoSilver™ Silver Stain Kit (Sigma PROTSIL1-1KT). AAV titer was determined by qPCR to normalize titers between different viral vector batches using LightCycler 480 SYBR Green I Master (Roche 04707516001) ...
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bioRxiv - Neuroscience 2024Quote: Neuropeptide Y levels in tissue were measured with commercially available ELISA kit (EMD Millipore, EZHNPY-25 K). Mice were anesthetized with isoflurane and euthanized by decapitation ...
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bioRxiv - Neuroscience 2024Quote: ... Linear fragments were then re-ligated using the Rapid DNA Dephosphorylation and Ligation Kit (Roche, Millipore Sigma): dephosphorylating the backbone fragment and ligating at room temperature for at least fifteen minutes.
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The subsequent steps in the proximal ligation assay were performed using the Duolink PLA Fluorescence kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Vaginal hydroxyproline content was used as a surrogate marker for collagen.10 The hydroxyproline assay kit (Sigma) was used according to manufacturer’s direction ...
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bioRxiv - Microbiology 2024Quote: The amount of β-lactamase was measured using a β-lactamase Activity Assay Kit (Sigma-Aldrich, US). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial and cytoplasmic protein was isolated using the mitochondrial/cytoplasmic fractionation kit from Millipore Sigma (cat.#: MIT1000). Primary antibodies used include the following ...