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Citations for Millipore Sigma :
6251 - 6300 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The precipitate was discarded and the supernatant was filtered through a 0.45 µm membrane (Millipore/Merck KGaA, USA).
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bioRxiv - Developmental Biology 2024Quote: ... Membrane was then incubated with either a 1:4000 dilution of a GFP specific primary antibody (Sigma; G1544) or a 1:3000 dilution of a RFP specific primary antibody (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... membranes were probed overnight at 4°C with primary antibodies against the respective target: ATF7IP (HPA023505, Sigma-Aldrich), CFLAR (#56343 ...
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bioRxiv - Developmental Biology 2024Quote: ... up to three S9-S9+ limbs were cultured over one 0.45 µm pore Durapore membrane filter (SLGVR33RS, Millipore) floating on DMEM medium (12100046 ...
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bioRxiv - Microbiology 2024Quote: ... subjected to precast 4-20% gels for SDS-PAGE and then transferred to 0.22 μm PVDF membranes (Millipore). After blocking with 5% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was then transferred to a methanol soaked polyvinylidene difluoride (PVDF) membrane (Millipore Immobilon FL 0.45 µm #IPFL00010). The membrane was then PBS washed ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were separated via SDS-PAGE (4-20% gradient, Bio- Rad) and transferred to a PVDF membrane (Millipore) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were separated by SDS-PAGE and transferred to Immobilon-P polyvinylidene difluoride membranes (IPVH00010; EMD Millipore). Immunoblotting analysis was performed with the indicated antibodies ...
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bioRxiv - Immunology 2024Quote: Total protein (40 µg/lane) was separated by SDS-PAGE and transferred onto the PVDF membrane (Millipore, USA). After the membranes were blocked with 3% BSA in PBST containing 0.05% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... Following electrophoresis, proteins were transferred overnight (40 V, 4°C) to a PVDF membrane (Millipore Sigma-Aldrich, #IPVH00010) in Tris-glycine buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated using 12% polyacrylamide gels and transferred to an Immobilon-P membrane (Millipore, Burlington, MA, USA) for 30 min using Towbin’s transfer buffer in a semi-dry transfer system as described in Zafeiropoulou et al (27) ...
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bioRxiv - Biochemistry 2024Quote: ... The solubilized membranes were centrifuged again and the dark green supernatant was incubated with FLAG-beads (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... Neutralized conditioned media and LDM4 media control were filter sterilized (0.22µm PVDF membrane, Steriflip, EMD Millipore, Burlington, MA), aliquoted ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked in 5% milk (skim milk powder, LP0031, Oxiod) in 1× PBS (P1379, Sigma-Aldrich), incubated with a primary antibody diluted in 5% milk in 0.1% PBS-Tween 20 (PBST ...
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bioRxiv - Microbiology 2024Quote: ... The gel was then wet-transferred to a 0.2 μM Immobilon-PSQ PVDF membrane (Millipore, cat. no. SEQ00010) at 200 mA for 90 min ...
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bioRxiv - Microbiology 2024Quote: ... PVDF membranes were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.1%Tween20 (Sigma-Aldrich) (TBS-T ...
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bioRxiv - Immunology 2024Quote: ... spanning the Chlamydia muridarum CPAF S491A antigen (final concentration of 2.5μg/ml)27 on PVDF-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFNγ (AN18) ...
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bioRxiv - Microbiology 2024Quote: ... PVDF membranes were incubated with rabbit anti-FLAG primary antibody (Sigma-Aldrich, cat. #F7425, at 1:500 dilution) diluted in TTBS with 0.5% (w/V ...
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bioRxiv - Immunology 2024Quote: ... and 10 μg protein was subjected to SDS-PAGE and blotted onto Immobilon-P PVDF membranes (Millipore #ISEQ00010). Nonspecific antibody binding was blocked with incubation in a solution of 2% BSA (Sigma-Aldrich # A7906) ...
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bioRxiv - Molecular Biology 2024Quote: Membranes were developed for 1-5 seconds using an enhanced chemiluminescence reagent (Luminata Forte HRP substrate; Millipore Sigma) in a gel documentation system (ChemiDoc Touch ...
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bioRxiv - Microbiology 2024Quote: ... Samples normalized for the protein content were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore). After washing with TBS buffer twice ...
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bioRxiv - Bioengineering 2024Quote: ... To determine the effect of the non-membrane permeable CTSB inhibitor CA-074 (Millipore Sigma, catalog no. 205530), the inhibitor was added directly to the co-culture at the indicated concentrations ...
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bioRxiv - Cancer Biology 2024Quote: ... 10-50 μg total protein was fractionated by SDS-PAGE and transferred to PVDF or nitrocellulose membranes (Millipore). Blocking was achieved with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was filtered through 0.22 μm pore size membrane filters (Whatman filters, Sigma-Aldrich, St. Louis, USA) to obtain a filtrate for infecting E ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% skim milk for mouse anti-Actin-β (1:10,000, A1978; Sigma-Aldrich Co.), and 5% BSA (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... outgrowing osteoblasts were seeded on Cellmatrix Type 1A (Nitta Geratin) placed onto a Millicell® membrane (Sigma-Aldrich) in α-MEM with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was cleared using ultracentrifugation at 200,000 g for 30 min and filtered using 0.65 μM PVDF membranes (Millipore). Clarified lysate was bound in batch to 1 ml Strep-Tactin Sepharose Resin (IBA Lifesciences ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred to 0.45 µm pore size polyvinylidene fluoride (PVDF) membranes (Millipore, Boston, MA, USA) using the wet transfer method with a Mini Trans-Blot Cell (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: To assess the filtration capacity of Hollow-membrane (HM) filters and Sterivex filters (EMD Millipore, Burlington,MA, USA) of the same nominal pore size (0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... A lipid membrane was created with the use of 7.6 g/L egg yolk lecithin (440154; Sigma-Aldrich) and 2 mM cholesterol (C8667 ...
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bioRxiv - Biochemistry 2024Quote: Samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated with 3% skim milk dissolved in phosphate-buffered containing 0.05% Tween 20 (PBS-T) ...
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bioRxiv - Biochemistry 2024Quote: ... the membranes were incubated with a goat polyclonal HRP-conjugated anti-mouse IgG (Millipore, 12-349, 1/5000) or goat polyclonal HRP-conjugated anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Bioengineering 2024Quote: ... the nanoporous membrane was modified with a 15-minute incubation of 1% (3-Aminopropyl)triethoxysilane (APTES, Sigma-Aldrich) and sandwiched between oxygen plasma-treated PDMS chips ...
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bioRxiv - Cancer Biology 2024Quote: ... 24 hours later the media was harvested and filtered through a Stericup 0.45 mm PVDF membrane (Millipore, SCHVU01RE), and transferred to ultra-clear centrifuge tubes (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... the membrane was exposed to a 1 mL solution of hydrochloric acid (HCl, 1 mol/L, Sigma-Aldrich) within a vacuum chamber for 6 hours to ensure thorough protonation.
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bioRxiv - Cell Biology 2024Quote: ... and 0.1% SDS) at room temperature for 20 min and then transferred to PVDF membranes (Immobilon-P, Millipore). Membranes were probed with anti-HA or anti-DYKDDDDK antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... 50 or 30 kDa nominal molecular weight cut-off (MWCO) Biomax® polyethersulfone ultrafiltration membrane disc (Millipore-Sigma) was washed by soaking it for 16 h in diethylpyrocarbonate (DEPC)-treated water (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 50 or 30 kDa nominal molecular weight cut-off (MWCO) Biomax® polyethersulfone ultrafiltration membrane disc (Millipore-Sigma) was washed by soaking it for 16 h in diethylpyrocarbonate (DEPC)-treated water (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... for peroxidase detection using the Syngene G:Box imager or a 0.45 µm pore LF-PVDF membrane (Sigma, IPFL00010) for fluorescent detection using the Licor Odyssey CLx Imager ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were coated with 1 µg/µl Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (Sigma-Aldrich) to allow cell adherence ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were separated by SDS-PAGE and transferred to Immobilon®-P PVDF Membrane (0.45mm pore size; Millipore) per standard protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Glycoproteins were dot blotted (two dots each) onto a PVDF membrane (Immobilon P, 0.22 µm pore, Merck Millipore), and N- and O-glycans were sequentially released ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked in 2% nonfat milk (Lab Scientific bioKEMIX 978-907-4243) in 1X TBS (Sigma T5912) for 30 minutes at room temperature while rocking ...
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bioRxiv - Cancer Biology 2024Quote: ... An equal amount of protein was resolved by 10% SDS–PAGE transferred onto PVDF or nitrocellulose membranes (Millipore), blocked with 5% nonfat dry milk for 1 hr and probed with primary Abs against phospho- and total p44/42 MAPK (ERK1/2) ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated by 10-15% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore #ISEQ07850, Billerica, USA), followed by blocking with Blocking One (Nacalai Tesque #03953-95 ...
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bioRxiv - Cancer Biology 2024Quote: ... The separated proteins were then electroblotted to methanol–activated polyvinylidene fluoride (PVDF) membranes (Sigma-Aldrich, Cat. No. IPVH00010). Upon transfer ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO, USA, Cat# A7906) in Tris-Buffered Saline with Tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were separated on SDS-PAGE gels and transferred to Immobilon-P transfer membranes (cat# IPVH00010, Merck Millipore). The following antibodies were used to probe the membranes ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were then separated by 12% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA) at 300mA for 90min ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed using ice-cold radioimmunoprecipitation assay buffer containing protease inhibitor cocktail (Sigma, P8340) and phosphatase inhibitor (Sigma ...