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Citations for Millipore Sigma :
6201 - 6250 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and KDEL) or 10% (GAPDH) SDS□PAGE and transferred to an Immobilon-P transfer membrane (Millipore, Billerica, MA). For PXR ...
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bioRxiv - Immunology 2023Quote: ... Serum was filtered through a Microcon-10kDa Centrifugal Filter Unit with Ultracel-10 membrane (Millipore Cat. No. MRCPRT010) per company protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then stripped and re-probed with an 18s rRNA targeting probe (5’-CCATCCAATCGGTAGTAGCG-3’, Sigma) which was used for signal normalisation.
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bioRxiv - Cell Biology 2023Quote: ... 30 µg of total protein extracts were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). After the nonspecific binding sites had been blocked ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples from exponentially growing cells were separated on a SDS–polyacrylamide (37.5:1) gel electrophoresis and blotted on 0.45µm pore PolyVinyliDenFluoride (PVDF) membranes (Immobilon-P from Sigma Aldrich). Membranes were blocked for 2 hours with 1x Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were dried and incubated in lysis buffer containing 7 µg/ml proteinase K (PK, Sigma‒ Aldrich) at 37 °C for 90 min ...
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bioRxiv - Cell Biology 2023Quote: ... Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore, IPVH00010). The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were separated on denaturing polyacramide gels before transfer to a 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of an overnight culture was then inoculated on 0.45 µm filters (MF-Millipore® Membrane Filter) on TSA and cultured at 37°C for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked for 1 hours using TBS-T (0.1% Tween-20) supplemented by 5% BSA (Sigma), and incubated overnight at 4°C with the following primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed and incubated in HRP-conjugated secondary antibody before detection using Immobilon ECL Ultra substrate (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 3 h at 22°C in Anti-mono-ADP-Ribose Binding Reagent (Sigma, MABE1076 ...
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bioRxiv - Molecular Biology 2023Quote: ... and concentrated on a 1,000 kDa MWCO tangential flow filtration cassette (regenerated cellulose membrane; Pellicon XL 50, Millipore). The concentrate was further concentrated using a centrifugal ultrafiltration unit (100 kDa MWCO ...
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bioRxiv - Cell Biology 2023Quote: ... Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore; 05317) using a wet blot transfer system (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Genetics 2022Quote: ... The membrane was then incubated overnight at 4°C with anti-H3 [1:5,000 (07-690, Sigma-Millipore)] ...
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bioRxiv - Genetics 2022Quote: ... The membrane was then incubated overnight at 4°C with anti-H3 [1:5,000 (07-690, Sigma-Millipore)] ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were separated on GenScript SurePAGETM Bis-Tris gels and blotted on the Immobilon-P transfer membrane (Millipore). The membrane was blocked with 5% skim milk and incubated with primary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked with 5% skimmed milk powder diluted in PBS-Tween 0.1% (v/v) (Sigma-Aldrich, #PP9416) (PBS-T) ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were run on NuPAGE 4%–12% Bis-Tris Gel (Novex) and transferred onto PVDF membranes (Millipore Sigma) and probed with antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then cut and the upper half was incubated in 1:1000 Cep89 antibody (Sigma, HPA040056), the bottom half in B-actin antibody 1:2000 (CST ...
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bioRxiv - Molecular Biology 2023Quote: ... The upper part of the membrane (26 – 250 kDa) was incubated in primary antibody against actin (Sigma-Aldrich) with a dilution of 1:4,000 in TBS-T for 1 h at RT on a rocker ...
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bioRxiv - Developmental Biology 2023Quote: ... all samples were filtered in a 47 mm polytetrafluoroethylene polymer (PTFE) membrane (0.45 μm pores, Merck Millipore, USA) and rinsed with filtered ultra-pure water at least three times in order to prevent any NaClO and KOH contamination ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then concentrated to 10 mg/ml with a 10 kDa Amicon Ultra membrane concentrators (Millipore) and loaded onto a Superdex S200 increase 10/300 GL size-exclusion column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane strips were connected to a glass fiber conjugate pad (G041 SureWick, Millipore Sigma, Oakville, Ontario, Canada) on one end and were sandwiched between three absorbent pads (Electrophoresis and Blotting Paper ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416 ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were then electroblotted to methanol–activated polyvinylidene fluoride (PVDF) membranes (Sigma-Aldrich, Cat. No. IPVH00010). Upon transfer ...
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bioRxiv - Biochemistry 2023Quote: ... The 139H2 membranes were washed again with TSMT and incubated with α-mouse IgG secondary antibody (A2304, Sigma) diluted 1:8000 in TSMT with 1% BSA for 1 h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... the membranes were treated in the dark with the reagent “Immobilon Forte Western HRP substrate” (Sigma Aldrich, WBLUF0100) and visualized at ChemiDoc instrument (BIO RAD).
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bioRxiv - Cell Biology 2023Quote: ... membranes spotted with different lipid species were blocked with 3% fatty acid free BSA (w/v; Sigma, 7030) in 0.1% (v/v ...
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bioRxiv - Genetics 2023Quote: ... The eluted samples were separated by SDS- PAGE and transferred to the PVDF membrane (Millipore, catalogue no. IPVH00010). Proteins were detected by immunoblotting using MBP antibody and S3L-RNase antibody.
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bioRxiv - Neuroscience 2023Quote: ... PVDF membranes were submerged for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich, St. Louis, MO, USA) in 40% ethanol and 10% acetic acid ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were probed overnight at 4°C with antibodies for SLC35A2 (Sigma Aldrich HPA036087 Rb 1:100, polyclonal) and GAPDH (Abcam Mouse 1:1000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... membranes were soaked in blocking solution +0.02% Tween 20 with 0.15 ug/ml GFP antibody (Millipore-Sigma 11814460001) for 30 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... membranes were soaked in blocking solution +0.02% Tween 20 with 0.15 ug/ml GFP antibody (Millipore-Sigma 11814460001) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Membrane fractions were resuspended and incubated in MELB supplemented with 0.5 mM PEG-maleimide (5 kDa, Sigma-Aldrich) and cOmplete protease inhibitors (Roche ...
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bioRxiv - Genetics 2023Quote: ... for the ciliary membrane and a mouse anti-gamma tubulin antibody (clone GTU-88 1:5000, Sigma Aldrich) for the basal body ...
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bioRxiv - Developmental Biology 2023Quote: ... Following transfer membranes were either blocked in 5% dried milk (Marvel) in PBST or 5% BSA (Sigma Aldrich). Membranes were incubated with primary antibodies (Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibodies against KRAS4B (WH0003845M1, Sigma Aldrich, 1 μg/ml) or HSC70 (sc-7298 ...
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bioRxiv - Biochemistry 2023Quote: ... 10% (w/v) sucrose] supplemented with 100 µM concentration of the membrane-impermeant fluorophore 5(6)-carboxyfluorescein (Sigma) at 45 °C to form liposomes with a final lipid concentration of 8 mM ...
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bioRxiv - Microbiology 2023Quote: ... and the membranes were blocked with a 1:1 mixture of 1XPBS/Tween-20 solution (Sigma-Aldrich #P3563) and Intercept blocking buffer (LI-COR Biosciences #927-70001 ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μm in diameter and spaced 200 μm apart) covered with a Breathe Easier sealing membrane (Sigma Aldrich). Data were acquired with the Multiwell Screen software (Multi Channel Systems ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Bioengineering 2024Quote: ... To determine the effect of the non-membrane permeable CTSB inhibitor CA-074 (Millipore Sigma, catalog no. 205530), the inhibitor was added directly to the co-culture at the indicated concentrations ...
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bioRxiv - Cancer Biology 2024Quote: ... 10-50 μg total protein was fractionated by SDS-PAGE and transferred to PVDF or nitrocellulose membranes (Millipore). Blocking was achieved with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were separated via SDS-PAGE (4-20% gradient, Bio- Rad) and transferred to a PVDF membrane (Millipore) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... Samples normalized for the protein content were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore). After washing with TBS buffer twice ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was filtered through 0.22 μm pore size membrane filters (Whatman filters, Sigma-Aldrich, St. Louis, USA) to obtain a filtrate for infecting E ...