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Citations for Millipore Sigma :
6051 - 6100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with a secondary Ab against immunoglobulin G (IgG) conjugated to horseradish peroxidase (Sigma-Aldrich) in PBST buffer (137 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μg of protein extract per sample was loaded on acrylamide gel and transferred to PVDF membrane (Millipore). All lysis buffers contained a cocktail of protease inhibitors and phosphatase inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... 4 relieving cuts were made in the retinas and they were flat-mounted on cellulose membranes (Millipore HABG01300) on glass slides and coverslipped with Fluoromount-G (Southern Biotech).
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated in blocking solution consisting of 5% v/w Skim Milk Powder (Sigma-Aldrich #70166-500G) in PBS with 0.05% Tween 20 (PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... and then transferred to an activated Immobilon-P PVDF Membrane (Merck Millipore Ltd, IPVH00010 PORE SIZE: 0.45 µm). The membranes were blocked using 5% nonfat dry milk in PBS/0.1% Tween20 for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Proteasome complexes were subsequently blotted for 1 h at 200 V on ice onto PVDF membranes (Merck Millipore) and further handled as described above.
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to PVDF membranes and probed with M2 anti-FLAG antibody (Sigma; 1 in 5,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Bioengineering 2022Quote: ... lymphatic endothelial cells (~106 cells) were seeded on a Corning™ Transwell Membrane Inserts with 0.4um pores (Sigma). After 24 hrs of culture a lymphatic monolayer was established on the transwell insert ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were then incubated overnight at 4 °C with primary antibodies against catalase (Sigma, Sao Paulo, Brazil), Nrf2 ...
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bioRxiv - Genomics 2022Quote: ... membranes were incubated for 5 min with one volume (generally 310 μl) of phenol:chloroform (Sigma, Cat. No. P3803). The membrane backers were removed from the tube with tweezers leaving just the mostly dissolved EVA membranes and tissue behind ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 µg retina protein extracts were separated on 8% polyacrylamide gels and blotted onto PVDF transfer membranes (Millipore) followed by blocking with non-fat dried milk (Applichem) ...
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bioRxiv - Cell Biology 2022Quote: ... Infectious media containing virus was collected 40 hrs later and filtered with a 0.45μm PES membrane filter (Millipore). Live filtered virus was used to transduce cells with polybrene (10μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was blotted with the following primary antibodies: MAB 2166 (anti-HTT, 1:1000, Millipore Sigma, USA), MW1 (anti-poly-Q ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated with the appropriate secondary antibodies (anti-mouse or anti-rabbit, 1:5000; Sigma, Italy) for 1h at RT ...
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bioRxiv - Synthetic Biology 2022Quote: ... Further processing by filtering was done by using a 13 mm diameter sterile syringe filter with a 0.45 μm or 0.2 μm pore size hydrophilic PVDF membrane (Millex-HV Syringe Filter, Millipore). All feces homogenizations were done using the eSwab™ buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then separated in 12% gel by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, ISEQ00010), which were then block with 5% milk in TBS-T buffer at room temperature for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were incubated with primary antibodies (Supplemental Information) diluted 1:1000 in 1% fish gelatin (Sigma Aldrich, G7041)/TBST overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with the following primary antibodies: anti-PSD-95 clone 6G6-1C9 (1:1000, MAB1596, Millipore), anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants of BMDMs were 5-fold concentrated using the Amicon Ultra-0,5 Membrane Ultracel-10 columns (Merck Millipore) and the IFNβ concentration was evaluated using the VeriKine Mouse Interferon Beta HS ELISA Kit (PBL assay science).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked for 1 hour in either 5% milk or 5% Bovine Serum Albumin (BSA) (Sigma, A7906) diluted in PBS/0.2% Tween ...
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bioRxiv - Microbiology 2022Quote: ... Groundwater samples were filtered with a membrane filter (type GPWP, 0.22 µm pore size: Merck Millipore, Darmstadt, Germany) in a pressure-resistant stainless filter holder (HP Filter Holder ...
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bioRxiv - Plant Biology 2022Quote: ... total proteins were separated on 8.5% SDS-PAGE gel and transferred to an immobilon-P (PVDF) membrane (Millipore). Membranes are then saturated with blocking buffer (TBS 1x ...
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bioRxiv - Bioengineering 2022Quote: ... The activated membranes were immediately submerged in a solution of 2% bis[3-(trimethoxysilyl)propyl]amine (Sigma-Aldrich) in 1% water and isopropyl alcohol ...
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bioRxiv - Neuroscience 2021Quote: ... the membrane was placed in deionized water and gently dried before adding chemiluminescent HRP substrate (EMD Millipore, WBKLS0100) for visualizing proteins ...
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bioRxiv - Biochemistry 2020Quote: ... were separated by SDS-PAGE and protein was transferred to MeOH-activated Immobilon®-P membrane (Merck Millipore) using Whatman paper and a transfer buffer of 48 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with the secondary antibody against rabbit coupled with a horseradish peroxidase (HRP) (Sigma-Aldrich) for 1 h in a 1:10000 dilution ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked for 1 h at room temperature in TBS-T (0.1%) - 5% milk (Sigma-Aldrich, Ireland). Primary antibodies were diluted in TBS-T (0.1% ...
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bioRxiv - Developmental Biology 2021Quote: ... The eluted protein was concentrated using ultrafiltration units with 10 kDa regenerated cellulose membrane (Amicon Ultra, Merck Millipore) and subsequently loaded on an equilibrated (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... full-length gB western blot was performed by transferring proteins to Immobilon-P membranes (Millipore Biosciences, Temecula, CA) and blocked with 5% BSA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated via gel electrophoresis on 12 % polyacrylamide gel and transferred to a PVDF membrane (Sigma Aldrich) using a semi-dry blotting system ...
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bioRxiv - Molecular Biology 2020Quote: ... The fraction was concentrated by ultrafiltration (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane, EMD Millipore) and loaded onto a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... and the supernatant was filtered through a sterile syringe filter Millex-GP with a 0.22 µm hydrophilic Polyethersulfone membrane (Merсk, Millipore). The resulting lysate was stored at +4 °C for no more than one week.
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bioRxiv - Molecular Biology 2020Quote: ... Solution was filtered through a Millex 0.22-µm sterile syringe filter with PES membrane (EMD Millipore Cat# SLGP033RB) and aliquoted ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-α-Tubulin blotting was conducted after stripping the blotted anti-CA membrane by stripping buffer (#2504, Millipore). The membrane was then re-blocked ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysates were subjected to SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Merck Millipore; Darmstadt, Germany). After blocking the membranes with 5% skim milk (Morinaga ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with one of the following primary antibodies: anti-cyclin G2 (1:500, HPA034684, Sigma); Flag (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein per each sample were resolved by SDS-PAGE and transferred onto PVDF membranes (Millipore). The membrane was probed with rabbit monoclonal antibodies that recognize CD63 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on SDS-polyacrylamide gels (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore). Membranes were blocked in 5% milk or bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were incubated with the appropriate secondary antibody and detected with Luminata™ Crescendo Western HRP Substrate (Millipore) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... Proteins were electrophoretically separated on 4–12% SDS-PAGE gels and transferred to a PVDF membrane (Millipore, MA). Membranes were subsequently blocked with blocking buffer (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in 5% skimmed milk in PBS (pH 7.4) containing 0.01% Tween-20 (Sigma-Aldrich, P1379) for 1 h at room temperature and were incubated overnight at 4 °C with primary rabbit polyclonal antibodies against mouse MAGL (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... transferred to a PVDF membrane and probed with the monoclonal anti-V5 and anti-α tubulin antibodies (Sigma) at 1:2,000 and 1:5,000 dilutions ...
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bioRxiv - Neuroscience 2022Quote: ... Membrane was blocked using 5% SureBlock (LubioScience) diluted in 1X PBS containing 0.1% Tween-20 (PBST, Sigma Aldrich) for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were washed again with TBST three times and visualized with chemiluminescence using HRP substrate (Millipore, #WBKLS0500) or Pico (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed with TBS-T on the next day and incubated with HRP-conjugated secondary antibodies (Millipore) at room temperature for 1 hour and exposed to films after washing.
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose membranes and probed with 1:1,000 a-FLAG primary (Sigma-Aldrich, clone M2) and 1:5,000 a-mouse secondary (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... the protein was dialyzed overnight at 4° C (Cat. # D9527-100FT, 14 kDa MWCO cellulose membrane, Sigma Aldrich) into CP storage buffer (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the membranes were incubated for 1 h with a 1/1000 dilution of anti-polyHis monoclonal antibody (Sigma) and then for 1 h with a 1/10 000 dilution of anti-mouse– horseradish peroxidase conjugate (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: Equal amounts of total proteins were separated on 12% SDS-PAGE and transferred to PVDF membranes (Millipore, #IPVH00010). The concentration of proteins was determined by Bradford assay ...