Labshake search
Citations for Millipore Sigma :
6101 - 6150 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2020Quote: ... Equal amount of whole cell extracts were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore, #ISEQ00010). Membranes were blocked with 5% non-fat dry milk diluted in PBS-T (PBS 1x ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved by SDS-PAGE and electroblotted by wet transfer onto Immobilion-P membranes (Millipore Sigma, MA). Membranes were blocked in 5% milk in TBS-T (50 mM Tris pH 7.6 ...
-
bioRxiv - Pathology 2020Quote: ... Membranes were washed three times as above and then developed with BCIP/NBT liquid substrate system (Sigma-Aldrich). The protein concentration of the FIV was determined using a BCA assay (Pierce #23227 ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to PVDF membrane and probed with M2 anti-FLAG antibody (Sigma; 1 in 1,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Millipore). Membranes were blocked for 1 hour in PBST (1× PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... The proteins were separated on a 10% SDS-polyacrylamide gel and transferred onto an Immobilon PVDF membrane (Millipore). Membranes were blocked in 5% BSA/TBST at 4°C overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... 30μg of protein was used for SDS-PAGE and Western Blotting post transferring onto nitrocellulose membrane (Millipore; HATF00010). The blots were decorated with antibodies against GroEL (Enzo ...
-
Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: ... proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes (EMD Millipore, Thermo Fisher Scientific, US).
-
bioRxiv - Neuroscience 2020Quote: ... 6-8 dpf Islet2b:PA-GFP larvae were injected with BODIPY membrane dye (1nL of 1mg/mL; Sigma, D3821) into the space behind the right eye and underlying skin to demarcate retinal anatomy and facilitate subsequent targeting ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto a Protran nitrocellulose membrane and probed with antibody clone 12D10 against puromycin (Millipore MABE343). Subsequently ...
-
bioRxiv - Cell Biology 2021Quote: ... the liposome suspension was extruded through a polycarbonate membrane filter with a 200-nm pore size (Merck Millipore). Smaller liposomes were prepared by sonicating the liposome suspension ...
-
bioRxiv - Developmental Biology 2021Quote: Nuclear extracts were separated on a 7% SDS-PAGE gel and transferred to Immobilon polyvinylidene difluoride membrane (Millipore). The membranes were blocked with Tris-buffered saline Tween [10 mM Tris (pH 8.0) ...
-
bioRxiv - Plant Biology 2021Quote: ... 5 µl of the denatured protein sample was manually spotted onto an activated PVDF Immobilon-P membrane (Millipore) mounted on top of a paper stack (bottom up ...
-
bioRxiv - Genetics 2020Quote: ... boiled for 5 min and loaded onto 10% SDS-PAGE gels and transferred onto a PVDF membrane (Millipore). The membranes were blocked with 5% non-fat milk for 2 hr at room temperature ...
-
bioRxiv - Genetics 2020Quote: ... cells were UV-irradiated (60 J m-2) through isopore polycarbonate membranes containing 3-μm-diameter pores (Millipore). For CPD immunodetection ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell lysates (20-40 μg) were subjected to 8-15% SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The membrane was blocked using 5% milk in TBST buffer at room temperature for 1 h ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 12-20 % SDS–PAGE and transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA). The membrane was blocked with TBS-T (20 mM Tris-HCl ...
-
bioRxiv - Microbiology 2020Quote: ... The purified protein was centrifuged by a 20 kDa-cut-off membranes made by Millipore (Milford, MA, USA) until the volume was approximately 1 mL ...
-
bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 30 min at RT with anti-β-actin (1:10’000, A3854 Sigma-Aldrich), which is used as a normalizer.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were then washed three times with TBS-T and incubated with alkaline phosphatase-conjugated secondary antibodies (Sigma) in 5% milk in TBS-T for 30 min at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked with 5% milk and incubated overnight with primary antibodies: anti-Flag antibody (Sigma, F3165), anti-MGMT antibody (Proteintech ...
-
bioRxiv - Cancer Biology 2020Quote: ... An equal amount of proteins was loaded into SDS-PAGE gels and transferred to PVDF membranes (Millipore, MA). Membranes were blocked for 2 hours with 5% non-fat milk in PBST (0.1% Tween-20 in PBS) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were subjected to polyacrylamide gel electrophoresis and transferred onto Immobilion polyvinylidene difluoride membranes (Millipore, Bedford, MA). These membranes were then blocked with 5% milk or 5% BSA and probed with antibodies specific to RBM3 (Abcam Cambridge ...
-
bioRxiv - Biochemistry 2020Quote: ... equal amounts of protein per sample were subjected to SDS-PAGE and transferred to a PVDF membrane (Millipore). For immunoprecipitations ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
-
bioRxiv - Neuroscience 2020Quote: ... All proteins were resolved in 10 % to 12 % SDS-PAGE and transferred to Immobilon transfer membrane (EMD Millipore). Membranes were blocked in ChemiBlocker (EMD Millipore ...
-
bioRxiv - Neuroscience 2021Quote: ... 15 min) followed by filtering of the supernatant with a 0.45 μm Millex PVDF membrane filter (Merck Millipore). For subsequent FPLC purification (Fast Protein Liquid Chromatography ...
-
bioRxiv - Pathology 2021Quote: ... The membrane was washed three more times in TBST before addition of Immobilon Western Chemiluminescence HRP Substrate (Millipore) and visualization via film development ...
-
bioRxiv - Biochemistry 2021Quote: ... About 20 μg protein was loaded in 12% SDS-PAGE and transferred to PVDF (0.22 μM) membrane (Millipore). Membranes were incubated with primary antibodies including anti-β-actin (1:10,000 dilution ...
-
bioRxiv - Microbiology 2020Quote: ... followed by a mid-filter of 5 μm pore size (Mixed cellulose ester membrane filter; Merck Millipore, USA) and finally through a 0.22 μm collection filter (Cellulose mixed ester membrane filter ...
-
bioRxiv - Microbiology 2020Quote: ... Virus-containing supernatant was collected 48-72 hours post-transfection and filtered through 0.45um PES membrane filter (Millipore). For infection ...
-
bioRxiv - Microbiology 2020Quote: ... 100 μL of bacterial cell suspension was added dropwise onto an Isopore membrane polycarbonate filter (HTTP01300, Millipore, UK). The filter was trimmed beforehand with a razor blade to identify the surface with inoculum ...
-
bioRxiv - Microbiology 2020Quote: ... After transfer, the membrane was incubated for 1 hour in blocking buffer (PBS, 0.1% Tween 20 (Sigma, 93773), supplemented with 5% milk or 5% BSA according to antibody recommendation) ...
-
bioRxiv - Immunology 2020Quote: Protein lysates were separated on SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membrane (Millipore, Etobicoke, ON). Membranes were blocked and probed ...
-
bioRxiv - Molecular Biology 2020Quote: ... The samples were separated by SDS-PAGE and electrotransferred to a 0.45 μm Immobilon-P PVDF membrane (Millipore). The membrane was incubated overnight at 4°C with DCP5 antibodies (provided by Rémy Merret and Cécile Bousquet-Antonelli ...
-
bioRxiv - Immunology 2020Quote: ... The supernatant was subsequently filtered with a Centricon Plus-80 with Biomax-PB membrane (NMWL100,000) (Millipore, Bedford, MA) and stored in liquid nitrogen to be used as viral stock for each macaque made from her own PBMCs ...
-
bioRxiv - Immunology 2020Quote: ... centrifuged 20 min at 3000g and filter-sterilized using a 0.22 μm membrane vacuum filter (Express PLUS, Millipore). Filtered supernatant was loaded on a 5 ml MabSelect SuRe column (GE Healthcare ...
-
bioRxiv - Immunology 2021Quote: ... 10 micrograms cell lysate was subjected to SDS-PAGE and transferred onto an Immobilon polyvinylidene difluoride membrane (Millipore). Protein detection was carried out using mouse monoclonal Ab against IκBα (#4814 ...
-
bioRxiv - Microbiology 2021Quote: ... Culture suspensions were passed through Sterivex™ 0.22 µm membrane filter units (EMD Millipore Corporation, Billerica, MA, US) to capture cells ...
-
bioRxiv - Genomics 2020Quote: ... The membrane was washed and incubated in a 1/2500 dilution of the rabbit-HRP secondary antibody (Sigma). After washing ...
-
bioRxiv - Neuroscience 2021Quote: ... To strip membranes and re-probe with a different antibody ReBlot Plus strong antibody stripping solution (EMD Millipore) was employed according to the manufacturer’s instructions prior to re-blocking in 5% milk and staining ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were stripped by 2 x 7 min incubations in stripping buffer (6 M guanidine hydrochloride (Sigma: G3272) with 1:150 β-mercaptoethanol ...
-
bioRxiv - Microbiology 2021Quote: ... Protein samples were separated with a 10% SDS-PAGE (Peqlab) and transferred onto a PVDF membrane (Sigma-Aldrich) via a semi-dry blotter (Peqlab ...
-
bioRxiv - Neuroscience 2023Quote: ... 30-100 μg of protein were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Protran, Sigma-Aldrich). Membranes were blocked in PBS or TBS containing 3% BSA or 5% skimmed milk ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 1XPBS + 0,1% Tween (1XPBS-T) with non-fat dry milk (5%, Merck Millipore) for 30 minutes at RT and then incubated for 3 hours at RT with a primary antibody resuspended in 1XPBS-T with 5% milk ...
-
bioRxiv - Systems Biology 2023Quote: ... Proteins were then separated on 12% SDS PAGE and transferred to a PVDF membrane (Millipore Sigma, 0.2 μM). Membranes blocked with 5% milk were then probed with anti-HA (1:2000 ...
-
bioRxiv - Neuroscience 2022Quote: Isolated ex vivo rd1-Thy1-GCaMP6s retinas were mounted onto filter paper (0.45 mm nitrocellulose membranes, MF-Millipore) with an optical window with the ganglion cell layer facing up ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and KDEL) or 10% (GAPDH) SDS□PAGE and transferred to an Immobilon-P transfer membrane (Millipore, Billerica, MA). For PXR ...
-
bioRxiv - Immunology 2023Quote: ... Serum was filtered through a Microcon-10kDa Centrifugal Filter Unit with Ultracel-10 membrane (Millipore Cat. No. MRCPRT010) per company protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... The membrane was then stripped and re-probed with an 18s rRNA targeting probe (5’-CCATCCAATCGGTAGTAGCG-3’, Sigma) which was used for signal normalisation.