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Citations for Millipore Sigma :
6151 - 6200 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 30 µg of total protein extracts were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). After the nonspecific binding sites had been blocked ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples from exponentially growing cells were separated on a SDS–polyacrylamide (37.5:1) gel electrophoresis and blotted on 0.45µm pore PolyVinyliDenFluoride (PVDF) membranes (Immobilon-P from Sigma Aldrich). Membranes were blocked for 2 hours with 1x Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... water was filtered onto 47 mm diameter 0.2 μm pore-sized polycarbonate white membranes (Millipore, Merck, Darmstadt, DE), which were stored at −20°C for future analysis ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-protein samples were separated by electrophoresis using a handmade SDS-polyacrylamide gel (7.5 or 9% polyacrylamide) and transferred to a PVDF membrane (Immobilon-P, pore size 0.45 µm; Merck-Millipore). The membrane was probed with primary antibodies listed in Supplemental Table 3.
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bioRxiv - Evolutionary Biology 2023Quote: Changes in the potential across the cell membrane were quantified by the 3,3′-Diethyloxacarbocyanine iodide (DiOC2, Sigma-Aldrich) dye (65) ...
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bioRxiv - Biochemistry 2023Quote: ... The 139H2 membranes were washed again with TSMT and incubated with α-mouse IgG secondary antibody (A2304, Sigma) diluted 1:8000 in TSMT with 1% BSA for 1 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were then electroblotted to methanol–activated polyvinylidene fluoride (PVDF) membranes (Sigma-Aldrich, Cat. No. IPVH00010). Upon transfer ...
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bioRxiv - Cell Biology 2023Quote: ... Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore, IPVH00010). The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μm in diameter and spaced 200 μm apart) covered with a Breathe Easier sealing membrane (Sigma Aldrich). Data were acquired with the Multiwell Screen software (Multi Channel Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed and incubated in HRP-conjugated secondary antibody before detection using Immobilon ECL Ultra substrate (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 3 h at 22°C in Anti-mono-ADP-Ribose Binding Reagent (Sigma, MABE1076 ...
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bioRxiv - Molecular Biology 2023Quote: ... and concentrated on a 1,000 kDa MWCO tangential flow filtration cassette (regenerated cellulose membrane; Pellicon XL 50, Millipore). The concentrate was further concentrated using a centrifugal ultrafiltration unit (100 kDa MWCO ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were separated on denaturing polyacramide gels before transfer to a 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked for 1 hours using TBS-T (0.1% Tween-20) supplemented by 5% BSA (Sigma), and incubated overnight at 4°C with the following primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were dried and incubated in lysis buffer containing 7 µg/ml proteinase K (PK, Sigma‒ Aldrich) at 37 °C for 90 min ...
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bioRxiv - Cell Biology 2023Quote: ... Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore; 05317) using a wet blot transfer system (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of an overnight culture was then inoculated on 0.45 µm filters (MF-Millipore® Membrane Filter) on TSA and cultured at 37°C for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were developed using enhanced chemiluminescence (Lumi Light or Lumi Light-plus, Roche/Sigma-Aldrich, St. Louis, MO) following the manufacturer’s instructions.
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Equal amounts of protein were electrophoresed on a 12% SDS– polyacrylamide gel and transferred to PVDF membranes (Millipore). Blots were blocked for 1 hour at RT (room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were transferred onto sterile membrane culture inserts (Millicell-CM, Millipore; 0.4 μm pore size, 30 mm diameter). These were placed into either a glass-bottom coverslip petri dish or a six-well plate containing pre-incubated slice culture medium (1.5-2 mL per well ...
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bioRxiv - Molecular Biology 2022Quote: The PVDF membranes were blotted with the following antibodies: anti-β-actin-peroxidase (Catalog NO. A3854; Sigma-Aldrich), anti-EBNA1 mouse monoclonal antibody (Catalog NO ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered with 5.0-μm and 0.45-μm PVDF pore membrane filters (Millex-HP Syringe Filter; Merck Millipore) to remove bacterial cells ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001 ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were subjected to SDS polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (IPVH 00010, Millipore). The membranes were immunoblotted with the indicated antibodies and developed with Super Signal West Dura Extended Duration Substrate (34076 ...
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bioRxiv - Plant Biology 2022Quote: ... Either total or immunoprecipitated proteins were separated on a SDS-PAGE gel and transferred to PDVF membrane (Millipore) for immunoblot analysis using anti-HA (ab18181 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was then incubated overnight at 4°C with 1:2000 M2 Anti-FLAG antibody (Sigma-Aldrich) in 5% milk-PBST ...
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bioRxiv - Microbiology 2022Quote: ... membranes were incubated for 1 min with a 1:1 mix of solution A (2.5 mM luminol [Sigma] ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was filtered through 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA) and stored as 1.0 mL aliquots at −80 °C until crystallization experiments ...
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bioRxiv - Cell Biology 2022Quote: ... An equal 20-40 ug of proteins were separated by SDS PAGE and transferred to PVDF membranes (Millipore). Western blots were performed as standard procedures ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred to a 0.45 μm Immobilon-FL PVDF membranes (IPFL00010; EMD Millipore, Burlington, MA, USA) using the eBlot™ L1 Fast Wet Transfer System (GenScript ...
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bioRxiv - Microbiology 2024Quote: ... and the microorganisms of the experimental group were removed with the addition of 0.5 mL TCA and filtered onto 0.2 μm GSWP membranes (polyethersulfone, Millipore) after incubation ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Millipore, IPFL00010). PVDF membrane was masked with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 M dithiothreitol for SDS-PAGE separation and electrotransferred to a polyvinylidene difluoride membrane (Immobilon-FL; Merck Millipore) and detected by an antibody raised against synthetic peptides of Synechococcus elongatus PCC sp ...
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bioRxiv - Biophysics 2024Quote: ... The flow through was concentrated using centrifugal filters with a 30 kDa cutoff membrane (Amicon 30K, EMD Millipore) and applied to a Superdex 200 prep grade 26/600 column (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... Next day membranes were incubated with peroxidase conjugated anti-rabbit IgG raised in Goat (Sigma, 1:8000 dil.) and peroxidase conjugated anti-mouse IgG raised in Rabbit (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... and 1 mM NaN3 (pH 6.5) and concentrated using conical spin concentrator (10 kDa membrane cut-off, Millipore) to a solution at 80-100 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA loading was assessed by re-probing the membrane with radiolabeled mouse genomic DNA with ethidium bromide (Sigma). Quantification of the spots was performed using ImageJ 1.53a.
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bioRxiv - Microbiology 2023Quote: ... triplicate culture samples for each light and temperature condition were vacuum filtered onto 0.2 µm PES membranes (Millipore) mounted on Nalgene filtration cups ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were reprobed with the anti-actin 20-33 rabbit polyclonal antibody (1:10,000 dilution; Sigma Aldrich #A5060). For comparison of protease-resistant α-syn banding patterns ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples (5 to 25µg/lane) were separated by 12.5% – 15% SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Proteins were probed in Can Get Signal Solution1 (Toyobo ...
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bioRxiv - Genomics 2024Quote: ... The elution supernatant was passed through Amicon Ultra-0.5 Centrifugal Filter with Ultracel-100 regenerated cellulose membrane (Millipore) to remove the remaining SDS ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was incubated with the secondary antibodies (goat α-mouse, horse radish peroxidase conjugated, Sigma Aldrich, A5278). Signal was visualized using the SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... the proteins on the gels were transferred onto an activated polyvinylidene difluoride (PVDF) membrane (Immobilon from Millipore, # IPFL00005) and then blocked with 1X TBS-0.1% Tween-20-5% dry milk for 1 hour at room temperature on a rocker ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Bioengineering 2023Quote: ... then membranes were incubated overnight at 4 °C with rabbit anti-BCRP (Sigma-Aldrich, cat# ZRB1217, 1:1000) or mouse anti-active β-catenin (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... membranes were soaked in blocking solution +0.02% Tween 20 with 0.15 ug/ml GFP antibody (Millipore-Sigma 11814460001) for 30 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... membranes were soaked in blocking solution +0.02% Tween 20 with 0.15 ug/ml GFP antibody (Millipore-Sigma 11814460001) for 30 min ...