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Citations for Millipore Sigma :
5951 - 6000 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... LDH assay was conducted according to the manufacturer’s instructions (Sigma-Aldrich, St. Louis, MO). 500 µL of spent saliva were stored at - 80°C ...
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bioRxiv - Molecular Biology 2024Quote: PLA experiments were performed using Duolink® In Situ Detection assay from Sigma-Aldrich according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... MIC assays were conducted in both cation-adjusted Mueller-Hinton broth (caMHB, Sigma Aldrich) and synthetic wound fluid (SWF) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cystine-FITC uptake assay was conducted using 2μM Molecular Biotracker cystine-FITC (Sigma, USA) and incubated at 37°C for 30 min ...
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bioRxiv - Genetics 2023Quote: ... During immunoprecipitation assays, cells was lysed with RIPA (Beyotime, P0013D) containing protease inhibitors (Sigma), and the immunoprecipitations were performed using anti-Flag M2 beads (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... cells and tissues lysis was performed in radioimmunoprecipitation assay (RIPA) buffer (Millipore, Cat. No.) supplemented with 1x protease inhibitor cocktail (PIC ...
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bioRxiv - Synthetic Biology 2023Quote: ... All sort-seq and individual assays were performed in M9 minimal media (Sigma-Aldrich; 6.78 g/L Na2HPO4 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were normalized with Milliplex® MAP Assay Buffer (EMD Millipore, Billerica, MA) to 6 μg protein per 12.5 μL for cytokine analysis and 1 μg protein per 12.5 μL for the MAPK pathway analysis ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal ANTI-FLAG® M2 antibody used in Cut&Tag assay was from Sigma. Mouse CD4 T Cell Isolation Kit used for the enrichment of CD4+ T cells was from Stem cell ...
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bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured at indicated time point using the MTT assay (Sigma M2128). Formazan crystals were extracted using 10% Triton X-100 and 0.1N HCl in isopropanol ...
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bioRxiv - Bioengineering 2023Quote: ... the organoids were resuspended in 50–70 μL radioimmunoprecipitation assay lysis buffer (Sigma-Aldrich) supplemented with phosphatase inhibitor tablets (PhosSTOP ...
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bioRxiv - Neuroscience 2023Quote: ... The same assay was performed using the inhibitors of glycolysis (2-deoxyglucose 50mM, [Sigma]) and OxPHOS (Oligomycin 1µM ...
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bioRxiv - Biochemistry 2022Quote: Calcium ions content in FBS was quantified via a colorimetric assay (#MAK022, Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cartridge was loaded with assay drugs: 5 µM oligomycin (Sigma, cat. no 75351-5mg), 1 µM FCCP (Abcam ...
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bioRxiv - Genomics 2023Quote: ... the supernatants were collected and protein concentration was measured by Bradford assay (Sigma-Aldrich). Extracts were boiled in presence of SDS loading buffer and analyzed by Western blotting.
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bioRxiv - Immunology 2023Quote: ... and lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors (Sigma) for 2 min at 25 Hz at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Cell Biology 2023Quote: ... protein lysates were extracted from BMDMs using Radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, R0278) complimented with protease inhibitor (Roche Diagnostics ...
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bioRxiv - Plant Biology 2023Quote: ... ChIP-qPCR assay was performed using anti-FLAG-M2-Agarose beads (Sigma-Aldrich A2220) as described 17 ...
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bioRxiv - Neuroscience 2024Quote: ... They were homogenized in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, St. Louis, MO,USA), protease inhibitor cocktail (Sigma ...
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bioRxiv - Immunology 2024Quote: ... migration and invasion assays were performed using a transwell chamber (Millipore, Billerica, MA, USA). For the migration assay ...
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bioRxiv - Microbiology 2024Quote: ... Glucose was measured by mixing samples with the Glucose (HK) Assay reagent (Sigma-Aldrich) following the manufacturer’s instructions and absorbance was measured on a spectrophotometer at 410 nm.
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bioRxiv - Pharmacology and Toxicology 2024Quote: Tissue samples were homogenized in ice-cold radioimmunoprecipitation assay lysis buffer (Millipore Sigma, #R0278) containing a Halt protease and phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: Protein content in mitochondria-enriched fractions were estimated using Bradford Assay reagent (Sigma-Aldrich) followed by solubilisation as previously reported by Rawat et al21 ...
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bioRxiv - Molecular Biology 2024Quote: ... In situ proximity ligation assay (PLA) was performed using Duolink PLA technology (Sigma-Aldrich) according to the manufacture instructions ...
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bioRxiv - Genetics 2024Quote: Oligonucleotides used in the CD and pull-down assays were purchased from Sigma-Aldrich and are listed in Table 1 ...
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bioRxiv - Plant Biology 2024Quote: ... Co-immunoprecipitation (co-IP) assays were performed using anti-Myc beads (Sigma-Aldrich, A7470), following the described procedure15 ...
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bioRxiv - Plant Biology 2024Quote: ... Co-immunoprecipitation (co-IP) assays were performed using anti-HA beads (Sigma-Aldrich, A2095), following the described procedure15 ...
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bioRxiv - Microbiology 2024Quote: Immunoglobulins in mouse sera were quantified using a Milliplex Multiplex assay (Merck Millipore, UK). Immunoglobulin beads (IgA ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed twice with 200 μl of assay medium (DMEM powder, D5030, Sigma; resuspended in 1 L UF water ...
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bioRxiv - Physiology 2024Quote: ... and eNOS/TRPV4 were investigated using the Proximity Ligation Assay (PLA) from Sigma-Aldrich. ECs were initially blocked and then exposed to primary antibodies sourced from various species ...
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bioRxiv - Plant Biology 2024Quote: ... water was removed and replaced with ROS assay solution (100 μM Luminol [Millipore-Sigma] ...
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bioRxiv - Microbiology 2024Quote: ... Cells used for plaque assays – BSC40 and Vero cells – were grown in MEM (Sigma) with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: Proximity ligation assays were conducted with Duolink as recommended by the manufacturer (Sigma-Aldrich). Cells were seeded on coverslips and at 60% confluency they were transfected with Mfn2-myc and NCLX-HA overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... For Colony Formation Assay the cells were fixed with 4% paraformaldehyde (Merck Millipore #104002) diluted in PBS and stained with 2% crystal violet (Sigma-Aldrich #HT90132 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were subjected to the cell viability assay using MTT Reagent (Sigma-Aldrich, M2128). The cell viabilities post-hypoxic or post-normoxic culture were divided by those of pre-hypoxic culture to evaluate the difference in cell viabilities between normoxic and hypoxic conditions.
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bioRxiv - Immunology 2024Quote: ... supernatants were collected and infectivity was determined with reverse transcriptase activity assays (Sigma, 11468120910).
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bioRxiv - Cancer Biology 2024Quote: ... and monoclonal ANTI-FLAG M2 antibody for immunoprecipitation assays were purchased from Millipore Sigma. Anti-ubiquitin antibody (P4D1 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was measured via the bicinchoninic acid assay (Thermo Fisher and Sigma Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... the Proximity Ligation Assay (PLA) was carried out as per the manufacturer’s instructions (Sigma) using the rabbit PLA Plus and mouse PLA Minus antibody and a far-red detection kit ...
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bioRxiv - Cell Biology 2024Quote: Flow cytometry assays were conducted using a Guava easyCyte Flow Cytometer (Millipore Sigma-Aldrich), equipped with 405 nm ...
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bioRxiv - Cell Biology 2024Quote: Proteins were isolated from HBMECs using Radioimmunoprecipitation Assay buffer (RIPA) buffer (Sigma Cat# R2078) with a complete mini EDTA-free protease inhibitor cocktail (Roche Cat# 11836170001 ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were incubated with TBST-Blotto containing primary monoclonal mouse anti-FLAG M2 antibody (1:2,000 dilution, Sigma) or monoclonal mouse anti-GAPDH antibody (1:2,000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were probed overnight at 4°C with mouse anti-BRAF [clone 3C6] (Sigma-Aldrich, cat# WH0000673M1; RRID:AB_1840330) at 2.5 µg/ml dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to PVDF membrane (Immobilon-FL, pore size 0.45 μm, Millipore, catalogue number IPFL00010) for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... a fraction of Proteins was separated on 12% SDS-PAGE gels and transferred to Immobilon PVDF membranes (Millipore). The membranes were blocked in 5% milk or 5% BSA (depending on the primary antibody) ...
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bioRxiv - Cell Biology 2020Quote: Eluted samples were resolved on standard sodium dodecyl sulphate-polyacrylamide gels and transferred to polyvinylidene difluoride membrane (Millipore). Membranes were blocked in 3% BSA in 0.2% Tween-20 in PBS for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed twice for 5 min in PBS plus 0.1% (v/v) Triton X-100 (Sigma-Aldrich) before being blocked with a 1% (w/v ...