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Citations for Millipore Sigma :
5801 - 5850 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Mitochondrial membrane potential (ψm) was measured by using fluorescent probes JC-1 and Rhodamine 123 (Sigma Aldrich). For yeast and mammalian cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with 1X phosphate-buffered saline (PBS) containing 0.1% Tween-20 (Sigma-Aldrich, cat# P1379) (PBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with 1X phosphate-buffered saline (PBS) containing 0.1% tween-20 (Sigma-Aldrich, USA, #P1379) (PBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfer efficiency was assessed by incubating the membranes in 5 mL of Ponceau S (Sigma-Aldrich, P7170) for 5 min followed by distaining with Tris- buffered saline-0.05% Tween 20 (TBST) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed previously to incubation with the secondary anti-mouse HRP antibody (A0168 Sigma Aldrich) for an hour at RT and imaged with the Clarify Western ECL substrate solutions (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... Equal loading and even transfer was confirmed by staining the membranes with 0.1% fast green (Sigma-Aldrich). Membranes were blocked for 30 minutes in Tris-buffered Saline ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were developed by enhanced chemiluminescence detection system using Immobilon® Forte Western HRP Substrate (Millipore, #WBLUF0500) on Bio-Rad ChemiDoc™ Touch Imaging System.
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bioRxiv - Synthetic Biology 2023Quote: ... filtration was carried out using a 47 mm polycarbonate filter membrane having 10 μm pores (EMD Millipore). The filter membrane was then incubated with 5 mL of 8M Gdn-HCl for 5 min ...
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bioRxiv - Physiology 2023Quote: ... Membranes were briefly stained with a 0.1% Ponceau S solution (Sigma Aldrich, St. Louis, MO, USA, P3504) to ensure equal protein loading and for normalization of target protein expression ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Biochemistry 2023Quote: ... Transfer efficiency was assessed by incubating the membranes in 5 mL of Ponceau S (Sigma-Aldrich, P7170) for 5 min followed by destaining with Tris-buffered saline-0.05% Tween 20 (TBST ...
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bioRxiv - Bioengineering 2023Quote: ... unreacted biotin-pentylamine was removed by ultrafiltration with a 3 or 10 kDa MWCO membrane (Merck Millipore). The recovered upper residual liquid (∼100 μL ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 1% w/v skim milk with 0.05% v/v Tween 20 (Sigma-Aldrich) in PBS (1% milk PBS-T ...
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Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repairbioRxiv - Cell Biology 2023Quote: Proteins were separated on a by 12% SDS polyacrylamide electrophoresis and transferred to a PVDF membrane (Millipore). Membranes were incubated in 3% skim milk in wash buffer (2 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2023Quote: ... After blocking the membrane with 1% BSA in TBS and 0.1% Tween (Sigma-Aldrich, St. Louis, Missouri) (TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... Six to eight slices were put on a Millicell-CM Biopore PTFE membrane insert (Millipore, PICM 03050) and kept on slice culture medium containing 50% MEM ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was subsequently clarified using a polyethersulfone membrane filter with a 0.22 μm pore size (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... the samples were separated by SDS-PAGE and transferred to Immobilon-P polyvinylidene difluoride membranes (Millipore, WBKLS0500) with the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was washed with TBS-T and incubated overnight in 5% bovine serum albumin (Sigma, A6003)/TBS-T with 1:1000 anti-β-Catenin primary antibody (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was separated by 8%–12% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Millipore). Membranes were blocked in 5% BSA in TBS for 1 hour at room temperature and then incubated with primary antibodies overnight at 4C ...
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bioRxiv - Cell Biology 2023Quote: ... They were separated by SDS–PAGE followed by transfer to polyvinylidene fluoride membrane (EMD-Millipore, Billerica, MA). The membrane was blocked with 5 % non-fat milk in Tris buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then resolved by SDS–PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, IPVH00010). Blocking was performed with 5% skim milk (Megmilk Snow Brand ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane surface was treated with a 0.1M solution of 3,3’,4,4’-Benzophenonetetracarboxylic acid (Sigma-Aldrich, USA) at pH 7 for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Transfer efficiency was assessed by incubating the membranes in 5 mL of Ponceau S (Sigma-Aldrich, P7170) for 5 min followed by destaining with Tris-buffered saline-0.05% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... and transferred to a PVDF membrane for analysis using α-FLAG antibody (Sigma Aldrich #F3165, 1:10,000), and α-CtBP as the loading control (DNA208 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA) (Sigma-Aldrich) in Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2024Quote: ... the membrane was washed with TBS-T and incubated overnight in 5% bovine serum albumin (Sigma, A6003)/TBS-T with 1:1000 of the primary antibody (see the materials table for a list) ...
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bioRxiv - Biophysics 2024Quote: ... the cell membrane was permeabilized by incubating the cells with a cold 0.2% Triton X-100 (Sigma) in PBS solution for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of total protein were separated on 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The resulting membranes were blocked for 1 hour at 37°C with TBST buffer (12 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat. IPVH00010). Membranes were incubated with primary antibodies in 5% BSA in 1x TBST buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked in 5% skim milk for mouse anti-Actin-β (1:10,000, Sigma-Aldrich, #A1978), rabbit anti-ATG5 (1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to nitrocellulose membrane for immunodetection using mouse anti-puromycin (1:1000,12D10, MABE943 Sigma) and mouse anti-actin (1:25 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were separated using SDS-PAGE and subsequently transferred to a polyvinylidene fluoride membrane (PVDF, Millipore Corporation). Then blocked PVDF membrane with blocking buffer (5% fat-free milk ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked using 5% SureBlock (LubioScience) diluted in 1x PBS containing 0.1% Tween-20 (Sigma Aldrich) for 60 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The resulting mixture was then passed through a 0.45 µm membrane filter (Millex LG, Millipore, Billerica, MA) and diluted with methanol ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore). PVDF membranes were blocked with TBS-T (50 mM Tris ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... The separated proteins were transferred by electrophoresis to Immobilon-P polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA) and treated with diluted antibodies ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the pH was adjusted to 7.8 and was filtered on 0.45-µm membrane (Millipore, Burlington, Massachusetts, USA).
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bioRxiv - Biophysics 2024Quote: ... The cell membrane was permeabilized for 5 min using 0.2% Triton X-100 (Sigma-Aldrich, Poznań, Poland). Microtubules were labeled with anti-tubulin antibodies 1:200 in PBS (Monoclonal Anti-α-Tubulin antibody ...
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The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagybioRxiv - Cell Biology 2024Quote: ... The samples were subjected to SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010; EMD Millipore). The primary antibodies used for immunoblotting were rabbit polyclonal antibodies against FIP200 (17250-1-AP ...
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bioRxiv - Microbiology 2024Quote: ... T2 and T3 using hydrophilic polycarbonate membranes (5.0 µm and 47 mm diameter) (TMTP04700) (Millipore, Darmstadt, Germany). DNA was extracted once ...
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bioRxiv - Neuroscience 2024Quote: ... by SDS-PAGE electrophoresis and transferred onto Whatman Protran 0.22 µm nitrocellulose transfer membrane filters (Sigma-Aldrich) using a wet Mini Trans-Blot Cell (BIO-RAD ...
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bioRxiv - Molecular Biology 2024Quote: ... 30-50 μg of proteins were resolved on SDS-PAGE gel and transferred onto PVDF membranes (Millipore) by semi-dry transfer method (Bio-Rad Trans-Blot SD) ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was incubated for 1 hour with anti-FLAG antibodies (diluted at 1:2,500, Sigma-Aldrich). After three washes with TBST (Tris-buffered saline with Tween) ...
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bioRxiv - Microbiology 2024Quote: ... 1.2 mg bromophenol blue) were subjected to SDS–PAGE and transferred to polyvinylidene disulfide membranes (Merck Millipore). After blocking the membranes with Azure Fluorescent Blot Blocking Buffer (Azure Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was filtered through a 0.22-μm pore-size polyvinylidene difluoride (PVDF) filter membrane (Millipore, USA). Particles from the filtrate were concentrated by ultracentrifugation at 100,000 x g for 2 h at 4°C using an SW32Ti rotor in an Optima LE-80K centrifuge (Beckman Coulter) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were transferred to MultiScreenHTS-HV 96-well filter plates with 0.45 µm PVDF membranes (Merck Millipore), and ice-cold acetonitrile was added to each well to induce protein precipitation ...
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bioRxiv - Neuroscience 2024Quote: Proteins were separated by SDS-PAGE in 10% polyacrylamide gels and transferred to polyvinylidene difluoride membrane (Millipore) by electroblotting (40 V ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenates (40 μg of protein) were resolved by 7.5% SDS/PAGE and transferred to nitrocellulose membranes (Millipore), which were blocked with 3% bovine serum albumin (BSA ...