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Citations for Millipore Sigma :
5851 - 5900 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Evaluating signaling pathway inference from kinase-substrate interactions and phosphoproteomics databioRxiv - Systems Biology 2024Quote: ... Samples were transferred in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) and ice-cold acetonitrile was then added to the samples to induce protein precipitation ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was subsequently clarified using a polyethersulfone membrane filter with a 0.22 mm pore size (Millipore). Antibodies were first purified by CaptureSelect CH1-XL beads (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was subsequently clarified using a polyethersulfone membrane filter with a 0.22 mm pore size (Millipore) and purified using ANTI-FLAG M2 Affinity Gel (Millipore-Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... the pH was adjusted to 7.8 and was filtrated on 0.45-µm membrane (Millipore, Burlington, Massachusetts, USA).
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bioRxiv - Plant Biology 2024Quote: ... A duplicate set of gels were transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 μm, Millipore) via semi-dry transfer using the BIO-RAD Trans-Blot Turbo Blotting Instrument ...
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bioRxiv - Neuroscience 2024Quote: ... The prepared samples were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). The membranes were sequentially blocked with 5% skim milk in buffer containing 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... electrophoresed between 125 and 200 V for 30 min and wet-transferred to PVDF membranes (Millipore, IPVH00010) overnight (30 V ...
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bioRxiv - Neuroscience 2024Quote: ... Protein from electrophoresed gels was transferred to PVDF membranes with 0.45 µM pore size (Millipore, cat# IPFL00010) by wet transfer in Towbin’s buffer [25 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by 10% SDS‒PAGE and transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). After the membranes were blocked with 5% nonfat milk for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA) (Sigma-Aldrich) for phosphorylated proteins immunoblots in Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Overnight wet transfer at 20 V was used to transfer proteins onto methanol-activated PVDF membranes (Millipore). Membranes were blocked for 1 hour in PBS-T (137 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: The protein samples were subjected to tricine SDS‒PAGE and transferred onto PVDF membranes (Merck Millipore, USA). The membranes were immunoblotted with anti-AjRTMP1 rabbit polyclonal antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Then membrane was washed and incubated with horseradish peroxidase-conjugated goat anti-mouse IgG (1:7,000) (Sigma) at room temperature for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were separated by SDS–PAGE (4–20%) and blotted onto polyvinylidene fluoride membranes (Merck Millipore). Membranes were blocked for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1 h with Tris-buffered saline (TBS) containing 0.1% Tween 20 (Sigma-Aldrich) and 5% dry milk and further incubated for 1 h with primary antibodies diluted in TBS ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were washed with PBST and incubated with HRP-labeled anti-mouse secondary antibody (Sigma-Aldrich) and the signals were detected.
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bioRxiv - Microbiology 2024Quote: ... The seawater was pre-filtered through polycarbonate membrane filters (142 mm diameter, 3.0-μm pore size; Millipore) to remove eukaryotic cells ...
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bioRxiv - Genomics 2024Quote: ... The supernatant was filtered using 0.45 μm PVDF pore membrane filters (Steriflip, Merck Millipore, Burlington, MA, USA). The filtrate was mixed with an equal volume of polyethylene glycol solution (20% PEG6000 in 2.5 M NaCl ...
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bioRxiv - Immunology 2024Quote: ... Equal protein amounts were separated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, IPVH00010) by wet electroblotting ...
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bioRxiv - Immunology 2024Quote: ... Hydrophilic polycarbonate membranes with 8.0 µm pore size and 13 mm diameter (Merck Millipore, Burlington, MA, USA) were placed on the soaked pads ...
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bioRxiv - Immunology 2024Quote: ... Media were collected and concentrated using protein concentrators containing a polyethersulfone membrane (3K MWCO; Millipore, Burlington, MA). Protein concentration was measured using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated for 1 h with alkaline phosphatase conjugated secondary anti-rabbit antibody (A9919, Sigma Aldrich) (human cells and mBMDM ...
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bioRxiv - Genomics 2024Quote: ... DNA in solution was loaded onto a 0.1 μm MCE membrane filter (MF-Millipore, CAT#VCWP04700, USA), which floated over a 40% PEG8000 solution to concentrate and wash DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blotted with the antibody against β-actin for an equal loading control (1:5000, Sigma)32.
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bioRxiv - Cancer Biology 2024Quote: ... The protein was separated by 8%–12% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Millipore). Membranes were blocked in 5% BSA in TBS for 1 hour at room temperature and then incubated with primary antibodies overnight at 4 ℃ ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were decorated with primary antibodies overnight at 4°C: anti-Ubiquitin (Millipore, 05-944, 1:3000), anti-Diglycyl-Lysine (Lucerna ...
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bioRxiv - Cancer Biology 2024Quote: ... The blocked membranes were incubated overnight at 4°C with the anti-FLAG (Sigma, Cat No. F2555) and anti-V5 (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... the membranes with proteins were incubated with primary antibodies diluted in 4% bovine serum albumin (BSA [Sigma, A7030] in TBST containing 0.02% sodium azide [Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto PVDF membrane (Sigma, Immobilon-P, Cat. No. IPVH00010). The membrane was blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... This layer was released from the membrane using a sterile 16 mm blade scraper (Sigma-Aldrich, C6106) by gently scraping the layer from four edges of the culture device ...
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bioRxiv - Cell Biology 2024Quote: ... The cell extracts were resolved by SDS–PAGE and transferred to a PVDF membrane (Immobilon-P, Millipore). Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated in HRP-linked secondary antibody and imaged using Immobilon Forte Western HRP reagent (Millipore) and X-ray film (Amersham ...
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bioRxiv - Developmental Biology 2024Quote: ... Separated proteins were electrotransferred to Immobilon-P polyvinylidene difluoride membranes with 0.45-μm pore size (#IPVH00010; Millipore). Membranes were blocked with 5% skim milk in Tris-buffered saline (TBS ...
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bioRxiv - Developmental Biology 2024Quote: AGM tissue from E10.5 and E11.5 embryos was dissected and cultured on a 0.65 μm membrane filter (#DAWP04700, Sigma) at the gas-liquid interface for 48 hours in center well organ culture dishes (#353037 ...
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bioRxiv - Cell Biology 2024Quote: ... Next the membranes were washed extensively three times with FAT BLOT buffer containing 0.1% Tween20 (Sigma-Aldrich) [used for all subsequent wahes] and then incubated with anti myc (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: Proximity ligation assay was performed according to manufacturer’s protocol (Duolink™ PLA Technology, Sigma). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Transwell assays were performed in 24-well PET inserts (Millipore, 8.0-μm pore size) for cell migration ...
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bioRxiv - Molecular Biology 2020Quote: Proximity ligation assay (PLA) was performed using the Duolink in situ-fluorescence (Sigma DUO92101). For G9a and Sp1 interaction ...
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bioRxiv - Cell Biology 2020Quote: ... Protein lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer (Sigma Aldrich, St Louis, MO) as per manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Assays were carried out in black Nunc flat-bottomed microtitre plates (Sigma-Aldrich, #P8741), and technical triplicates were included for each sample ...
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bioRxiv - Evolutionary Biology 2021Quote: The assays were performed in 96 well microplates in M9 minimal media (Sigma-Aldrich) supplemented with MgSO4 ...
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bioRxiv - Neuroscience 2021Quote: ... using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (Loo 2011) (Sigma-Aldrich in situ Cell Death Detection Kit ...
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bioRxiv - Neuroscience 2021Quote: ... sertindole and spiperone used in competition assays were obtained from Sigma-Aldrich (Poole, UK). Olanzapine ...
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bioRxiv - Immunology 2022Quote: ... mature miR-31 was quantified using MystiCq® microRNA qPCR Assay (Sigma, MIRRM00-100RXN) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... The protein concentration was assessed using bicinchoninic acid assay (BCA, BCA1-1KT, Sigma Aldrich). A total of 10-40 μg of protein was loaded on 8-14% acrylamide gel ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA-PK assay additionally contained 2.5 μg/ml DNA (Sigma; Cat. # D-4522). Reactions were performed for 60 min at 30°C in FlashPlate Basic Microplate (PerkinElmer ...
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bioRxiv - Biochemistry 2020Quote: ... The PRKG1 and PRKG2 assays additionally contained 1 μM cGMP (Sigma; Cat. # G-6129). The DNA-PK assay additionally contained 2.5 μg/ml DNA (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... MYLK2 and MYLK3 assays additionally contained 1 μg/ml Calmodulin (Millipore; Cat. # 14-368) and 0.5 mM CaCl2 (VWR ...
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bioRxiv - Microbiology 2020Quote: The aconitase activity assay was performed according to the manufacturer’s protocol (MAK051, Sigma Aldrich). Briefly ...
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bioRxiv - Neuroscience 2021Quote: Snap-frozen whole cerebella were homogenized in radioimmunoprecipitation assay buffer (RIPA Buffer; Sigma–Aldrich) with complete Mini EDTA-free Protease Inhibitor Cocktail Tablets (Roche Diagnostics ...