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Citations for Millipore Sigma :
5751 - 5800 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The NC membrane was initially blocked with 5% nonfat milk and 2% BSA (A4503, Sigma (v/v)) in Tris buffered saline with 0.1% Tween 20 (93773 ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were resolved on 10% SDS–PAGE gels and transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked for 1 hour in blocking buffer [3% Skim milk (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... 10600002) or 0.2 µm or 0.45 µm PVDF membranes (Amersham Protron, 10600021 or Immobilon-P, Millipore, IPVH00010) for 1 h at 100 V in tris-glycine buffer with 10 % methanol ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were blocked at RT for 1 hour with 5 % milk diluted in PBS (Sigma-Aldrich, P4417) containing 0.1% Tween-20 (PBS-T) ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were loaded onto a 10% SDS-PAGE gel and transferred onto a PVDF membrane (Millipore). The membrane was blocked with 3% BSA (BioFroxx ...
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bioRxiv - Immunology 2024Quote: ... TEER values (Ω * cm2) were calculated as net resistance * membrane area (0.33cm2 for 24-well inserts, Millipore). Only samples with TEER > 200 Ω*cm2 on day 10 were used for stimulation experiments.
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bioRxiv - Neuroscience 2024Quote: ... Slices were then transferred onto membrane inserts with prewarmed organotypic slice culture (OTC) cultured MEM-based (Sigma) medium containing 0.00125% ascorbic acid (Roth) ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess IR800-streptavidin was washed from the membranes by four times with 0.1% TWEEN-20 (Sigma-Aldrich) in 1× PBS for 10 min/each at room temperature ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the WT SCO4122 and mutant M93L-SCO4122 proteins were transferred on to polyvinylidene difluoride (PVDF) membrane (Millipore) using transfer buffer (6g Tris base ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysates were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-P; Millipore). Membranes were blocked in 5% skim milk in 0.05% Tween-20 in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The protein samples were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Billerica, MA, USA). The membranes were incubated with primary antibodies against EI24 (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated in an SDS-PAGE 10% gel and subsequently blotted onto a PVDF membrane (Millipore). After incubation with blocking buffer (Tris-buffered saline with 5% nonfat milk) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 1% w/v skim milk with 0.05% v/v Tween 20 (Sigma-Aldrich) in PBS (1% milk PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... They were separated by SDS–PAGE followed by transfer to polyvinylidene fluoride membrane (EMD-Millipore, Billerica, MA). The membrane was blocked with 5 % non-fat milk in Tris buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were separated by SDS-PAGE and transferred to Immobilon-P polyvinylidene difluoride membranes (Millipore, WBKLS0500) with the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then resolved by SDS–PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, IPVH00010). Blocking was performed with 5% skim milk (Megmilk Snow Brand ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was subsequently clarified using a polyethersulfone membrane filter with a 0.22 μm pore size (Millipore).
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Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repairbioRxiv - Cell Biology 2023Quote: Proteins were separated on a by 12% SDS polyacrylamide electrophoresis and transferred to a PVDF membrane (Millipore). Membranes were incubated in 3% skim milk in wash buffer (2 mM Tris-Cl ...
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bioRxiv - Neuroscience 2023Quote: ... Six to eight slices were put on a Millicell-CM Biopore PTFE membrane insert (Millipore, PICM 03050) and kept on slice culture medium containing 50% MEM ...
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bioRxiv - Physiology 2024Quote: ... The lysates were then separated by SDS-PAGE and transferred to Immobilon 0.45mm membranes (Cat# IPVH00010, Millipore). Western blotting was conducted using specific various primary and secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... concentrated using Amicon Ultra-15 centrifugal filter units with an Ultracel-30 membrane (Millipore, Billerica, MA, USA), filtered through a 0.45 μM filter ...
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bioRxiv - Synthetic Biology 2024Quote: ... Microtiter plates were covered with a Breath-EasyTM sealing membrane (Sigma-Aldrich Co., St. Louis, MO, USA) and OD600 readings were recorded automatically under continuous shaking in an Elx808TM absorbance microplate reader (BioTek Instruments Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... After blocking the membrane with 1% BSA in TBS and 0.1% Tween (Sigma-Aldrich, St. Louis, Missouri) (TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane surface was treated with a 0.1M solution of 3,3’,4,4’-Benzophenonetetracarboxylic acid (Sigma-Aldrich, USA) at pH 7 for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Transfer efficiency was assessed by incubating the membranes in 5 mL of Ponceau S (Sigma-Aldrich, P7170) for 5 min followed by destaining with Tris-buffered saline-0.05% Tween 20 (TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was washed with TBS-T and incubated overnight in 5% bovine serum albumin (Sigma, A6003)/TBS-T with 1:1000 anti-β-Catenin primary antibody (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was separated by 8%–12% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Millipore). Membranes were blocked in 5% BSA in TBS for 1 hour at room temperature and then incubated with primary antibodies overnight at 4C ...
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bioRxiv - Bioengineering 2023Quote: ... unreacted biotin-pentylamine was removed by ultrafiltration with a 3 or 10 kDa MWCO membrane (Merck Millipore). The recovered upper residual liquid (∼100 μL ...
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bioRxiv - Biochemistry 2023Quote: ... Transfer efficiency was assessed by incubating the membranes in 5 mL of Ponceau S (Sigma-Aldrich, P7170) for 5 min followed by destaining with Tris-buffered saline-0.05% Tween 20 (TBST ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Microbiology 2023Quote: ... The holes were covered by attaching a square of PVDF filtration membrane (0.45 µm pore size, Millipore) on the inner face of the lid using clear PVA glue (UHU ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were probed with ECL substrate (100 mM Tris HCl pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and transferred to a PVDF membrane for analysis using α-FLAG antibody (Sigma Aldrich #F3165, 1:10,000), and α-CtBP as the loading control (DNA208 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... samples were electrophoresed under reducing conditions on SDS-polyacrylamide gels and transferred to PVDF membranes (Millipore #IPFL00010). Membranes were blocked with either 4% non-fat milk or fish gelatin (Sigma #G7041-100G ...
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bioRxiv - Physiology 2024Quote: ... and equal amounts were loaded for SDS-PAGE and transferred to Immobilon-Fl PVDF membrane (Millipore, IPFL00010). Each membrane was blocked for 1.5 hr at Room Temperature with Odyssey Blocking Buffer (TBS ...
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bioRxiv - Microbiology 2024Quote: ... was used as second antibody and proteins on the membrane were visualized using BCIP/NBT substrate (Sigma). When deer plasma specimens were used as primary antibody ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... of embryonic lungs were subjected to SDS-PAGE and transferred to a polyvinylidene difluoride membrane (PVDF; Millipore). Immunoblotting was performed using an anti-Nolz-1 antibody (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: Protein extracts were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Merck, Germany). Membranes were blocked with 5% non-fat milk and incubated with a specific anti-biotin antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Briefly, gels were transferred in CAPS buffer (10mM CAPS, 10% ethanol) to PVDF membranes (EMD Millipore #1PVH00010) and incubated with primary antibodies at 4°C overnight (ATF4 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was subsequently clarified using a polyethersulfone membrane filter with a 0.22 μm pore size (Millipore).
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to nitrocellulose membranes before blocking with 5% milk (Marvel) or bovine serum albumin (Sigma) w/v in PBS-T (0.1% Tween-20 in PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were washed with TBS-T and incubated with goat anti-mouse IgG-peroxidase conjugate (Sigma DC02L) for 1 h at room temperature and washed again ...
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bioRxiv - Bioengineering 2022Quote: The cells on the membrane were fixed with 4% paraformaldehyde (PFA; Sigma-Aldrich, St Louis, MO, USA) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble and insoluble fractions were analyzed by SDS–PAGE and immunoblotting onto PVDF membranes (EMD Millipore) were performed using standard protocols42.
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times in PBST before incubation with secondary antibody (Sigma-Aldrich; GENA931 or GENA934) diluted 1:5000 in PBST containing 5% Omniblok for 1 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Equivalent amounts of protein were separated by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Membranes were incubated with antibodies per manufacturer’s suggestions ...
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bioRxiv - Genetics 2022Quote: ... Membrane pieces were incubated in antibody solution {1xTBS with 1% milk and 0.2% Tween 20 (Sigma P7949)} with primary antibodies against GAPDH and CRMP2 (Supplementary Table 3 ...