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Citations for Millipore Sigma :
5901 - 5950 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The RNA isolation of the retina was carried out according to the manufacturer’s introduction using the Gene Elute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO, USA). For total RNA isolation of optic nerve tissue ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial mass was assessed by measuring citrate synthase activity from 4 million whole cells normalized to protein input (U/mg) in triplicate from three biological replicates using the Citrate Synthase Assay Kit (CS0720, Sigma-Aldrich, St, Louis, MO).
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bioRxiv - Cell Biology 2021Quote: ... the hiPSC-CMs were fixed and permeabilised with the FIX and PERM™ Cell Permeabilization kit and labelled with an antibody specific for α-actinin (1:250, Sigma-Aldrich #A7811), followed by an Alexa Fluor 350-conjugated secondary antibody (1:500 ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from callus fragments grown on selective medium for eight weeks using a commercial kit (Sigma, Spectrum™ Plant Total RNA). First strand cDNA was synthesised using polyA primers and RevertAid First Strand cDNA synthesis kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: The release of inorganic phosphate during 2C-mediated ATPase hydrolysis was measured using the Malachite Green Phosphate Assay Kit (Sigma Aldrich, Zwijndrecht, The Netherlands). Each 50 µL reaction comprised 500 nM recombinant 2C protein and 1 mM ATP in reaction buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: Nitrite concentration in the lung homogenate was measured (n=3 per group per time point) using Griess Reagent kit following manufacturer instructions (Sigma, St. Louis, MO, USA). Absorbance was recorded using a GloMax plate reader (Promega Corporation ...
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bioRxiv - Plant Biology 2021Quote: ... and ground to a fine powder (100-200 mg) prior to RNA isolation using the Spectrum™ Total Plant RNA Kit with DNase I digestion (Sigma, St. Louis, MO). The only modification to manufacturer’s ‘Protocol B’ was that prior to the tissue lysis step ...
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bioRxiv - Biochemistry 2022Quote: ... we first extracted the genomic DNA (gDNA) from each cellular fraction using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay was carried out using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Millipore Sigma, St. Louis, MO); diaminobenzidine (DAB ...
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bioRxiv - Cell Biology 2021Quote: ... total cellular triglycerides were extracted from cells using the Folch extraction method and quantified by using a Serum Triglyceride Determination Kit (Sigma, St. Louis, MO, USA). The absorbance was read at 540nm using an Eppendorf Biospectrometer and 2.5 mg/mL glycerol was used as the standard (Sigma) ...
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bioRxiv - Microbiology 2020Quote: Cytokines in BAL fluid samples were measured (pg/ml) in singlicate by Luminex using a Millipore human cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA) according to manufactures instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentration of the supernatant was determined by comparison to a standard curve of bovine serum albumin (BSA) solutions using the bicinchoninic acid (BCA) assay kit (Catalog No. BCA-1, Sigma, St. Louis, MO, USA) and absorbance was read at 562 nm using a BioTek Synergy HT plate reader ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was collected and prepared for glycogen measurement alongside a 2 mg/mL glycogen standard using the Sigma glycogen assay kit (Catalog No. MAK016, Sigma, St. Louis, MO, USA) and read using a BioTek Synergy HT plate reader at 570 nm.
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bioRxiv - Biochemistry 2021Quote: ... Each cellular fraction was then expanded and harvested prior to the extraction of genomic DNA using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A semi-nested PCR approach was then used to selectively amplify the mutagenized region of the polyprotein reporter from the genomic integration site in a similar manner as was previously described (Matreyek et al. ...
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bioRxiv - Microbiology 2021Quote: ... Then, compressed air (DAN oxygen kit, Divers Alert Network, USA) and 25 µM of sodium sulfide (Na2S*9H2O; Sigma-Aldrich, St. Louis, MS, USA) were injected into the oxic and anoxic incubations ...
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bioRxiv - Immunology 2019Quote: ... Serum and lung immune factors were analyzed by multiplex technology (performed by Eve Technologies, Calgary, AB, Canada) using a 31 cytokine/chemokine/growth factor kit (Millipore, St Charles, MO, USA). The assay was run on a Bio-Plex™ 200 system (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Hydrogen peroxide from 40 hippocampus samples of mice of both strains (n = 5 per group) was measured as an indicator of oxidative stress and it was quantified using the Hydrogen Peroxide Assay Kit (Sigma-Aldrich, St. Louis, MI) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Genomic DNAs were prepared from selected isolates showing different cell and colony morphologies using GenElute™ Bacterial Genomic DNA Kit (Sigma Aldridge, Gillingham, UK). Identification to presumptive species level was carried out by performing PCR amplification of 16S rRNA gene sequences using primers TPU1 and RTU8 (73 ...
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bioRxiv - Immunology 2020Quote: ADCP assays were performed using ExpiCHO-S target cells transiently transfected with SARS-CoV-2 S and fluorescently labeled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich, Cat. Nr.: MINI67) as targets ...
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bioRxiv - Plant Biology 2019Quote: ... In situ PLA detection was carried out using the appropriate DUOLINK in situ Orange Kit Goat/Rabbit obtained from Sigma-Aldrich (Saint Luis, USA) according to the protocol of the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA was extracted at the field using quick Extract-N-Amp™ Tissue PCR Kit (Sigma-Aldrich Co. LLC, MO, Louis. St; USA) with some modifications ...
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bioRxiv - Pathology 2019Quote: ... and TGF-β1 in the samples was measured using magnetic bead-based multiple immunoassays (MILLIPLEX® MAP kit, Human High Sensitivity T Cell Magnetic Bead Panel: EMD Millipore Corporation, Germany). Each assay included two calibration curves for each of the proteins to be measured (calibration ranges ...
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bioRxiv - Genomics 2021Quote: ... genotyping for the Ile148Met variant associated with hepatic steatosis (rs738409) was performed on genomic DNA extracted from the blood buffy coat using the GenElute Blood Genomic DNA kit (Sigma, St. Louis, MO, USA). rs738409 was genotyped using validated primers and TaqMan probes (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ADCP was performed using CHO cells stably expressing full-length wild-type SARS-CoV-2 S glycoprotein (target cells) fluorescently labelled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich; Cat. Nr.: MINI67). Target cells were incubated with titrated concentrations of mAbs for 10 minutes ...
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bioRxiv - Genomics 2019Quote: ... Half of the sample was proceeded for genomic DNA isolation using GenElute plant genomic DNA miniprep kit (Sigma-Aldrich Co., St. Louis, MO, USA) according to the manufacturer’s instructions for PacBio and Illumina platforms sequencing ...
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bioRxiv - Immunology 2021Quote: ... Ten μl were removed from each tube to measure C8 or longer free fatty acids (i.e., palmitic acid released from PGPC or POVPC) (Sigma free fatty acid kit MAK044) and the remaining samples were stored at -80℃ prior to use or analysis.
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bioRxiv - Evolutionary Biology 2022Quote: ... 20 females from each biological replicate were flash frozen in liquid nitrogen and total RNA was isolated using the GenElute Mammalian Genomic RNA miniprep kit (Sigma Aldrich, CAT# RTN350-1KT), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: WT BBS and BBS Mut cells subjected to normoxic or hypoxic treatment were lysed using assay buffer provided in the Phosphofructokinase (PFK) Activity Colorimetric Assay Kit (Sigma, MAK093, lot no. 6E31K07760). An equal quantity of protein lysates was used to determine the total PFK activity as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the nuclear fraction was prepared from whole plants using the ‘Semi-pure Preparation of Nuclei Procedures’ protocol of the CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich, St. Louis, MO). Genomic DNA was isolated from the nucleus or whole plant using a DNeasy Plant mini kit (Qiagen ...
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bioRxiv - Physiology 2023Quote: ... and L-citrulline were quantified with HPLC-MS as previously described.49 Arginase activity was assessed with an arginase activity assay kit (Sigma Aldrich, St. Louis, MO) following manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II, Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... offspring were screened for Akt1 or Foxo4 mutations from genomic DNA from tail-tip biopsies using the REDExtract-N-Amp™ Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA). PCR was performed on the purified DNA samples using primers flanking the guide RNA sites ...
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bioRxiv - Physiology 2022Quote: ... triglyceride and glycerol (Triglyceride-SL Assay, Cat# 236-60, Sekisui Diagnostics, PEI, Canada) and pyruvate (Pyruvate Assay Kit, Cat#MAK071, Sigma-Aldrich, Oakville, ON, Canada) were measured using colorimetric assays according to the manufacturers’ directions ...
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bioRxiv - Plant Biology 2022Quote: ... Full-length FIN4 mRNA in PCRII-TOPO vector was used to synthesize sense and anti-sense probes using a DIG-labeled probe synthesizing kit (Millipore-Sigma, catalog number 11175025910) with T7 RNA polymerase for sense probe and SP6 RNA polymerase for antisense probe synthesis with 1μg plasmid as template ...
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bioRxiv - Microbiology 2024Quote: PCR products obtained from the first round of amplification (COMPETE, BLOCK, CONTROL, see section 2.2) were purified using the GenElute™ PCR Clean-Up Kit (Sigma Aldrich, St. Louis, MO). The purified DNA was diluted 10-fold with DNA free sterile water and used as a DNA template for a nested PCR to attach Illumina adaptors for Illumina sequencing ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from endothelial cells with the TRIzol reagent and Poly(A)+ RNA was purified using the GenEl-ute mRNA Miniprep Kit (Sigma, Louis, MO, USA; MRN10). The m6A concentration was assessed using the m6A RNA Methylation Kit (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... bile salts were supplied from bovine bile and measured using a commercial kit according to the supplier’s protocol (Sigma-Aldrich, cat. No. MAK 309). Finally ...
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was performed on paraffin-embedded sections and fifty-micron-thick free-floating frozen sections according to the manufacturer’s instructions but with the time of some steps lengthened to ensure good penetration of the reagents into the tissue (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Millipore-Sigma, Cat # S7110). Sections were post-fixed for ten minutes at room temperature in 2:1 ethanol:acetic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein solution was dialyzed overnight at 4°C in tubes of the Pur-A-LyzerTM Maxi 6000 Dialysis Kit (Sigma, cat. No. PURX60100-1KT) against dialysis buffer (20 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... were grown in ATCC’s vascular cell basal medium supplemented with microvascular endothelial cell growth kit-VEGF and 12.5 μg/mL blasticidine (Sigma-Aldrich, St. Louis, MO, 203351) for 48 h at 37°C + 5% CO2 until reaching 80-90% confluency ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue lysis was performed in Roche cOmplete Lysis-M reagents (lysis buffer and protease inhibitor cocktail contained in the kit, Millipore Sigma cat. Nr. 4719956001). Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat ...
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bioRxiv - Immunology 2023Quote: ... Blood Urea Nitrogen (BUN) was also determined in the same serum samples using a urea colorimetric assay kit (#MAK006, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from leaf samples (three biological replicates for each genotype) and using Spectrum Plant Total RNA Kit (Sigma Aldrich, Saint Luis, USA) following instruction ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2023Quote: LDH activity was quantified in the serum samples using a lactate dehydrogenase activity assay kit (catalog number MAK066, Sigma-Aldrich, St. Louis, MO, USA). The assays were performed according to the manufacturer’s instructions using serum samples diluted 1:10 in 1x Dulbecco’s phosphate buffered saline (1× DPBS ...
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bioRxiv - Immunology 2023Quote: L-lactate level was assessed in plasma using colorimetric assays according to the manufacturer’s protocol with slight modifications (Lactate Assay Kit, Sigma-Aldrich, Missouri, USA, # MAK064-1KT). 25 μl of lactate standards or plasma samples were mixed with an equal volume of Master Reaction Mix (consists of ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 1.5-week-old Arabidopsis seedlings pooling 3 seedlings/extraction for each transgenic lines and using Spectrum™ Plant Total RNA Kit (Sigma-Al-drich, STRN250), RNAs were on-column treated with the DNase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 16 were subjected to in-situ Terminal deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) using the ApopTag Plus Fluorescein Kit (EMD Millipore, Burlington, MA, USA), following previously established protocols (Maddala et al. ...
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bioRxiv - Immunology 2021Quote: ... At 0 min and 1 h the sample was analyzed for concentrations of inorganic monophosphates with the ATPase/GTPase Activity Kit (Sigma-Aldrich, Cat. no: MAK113-1KT) according to manufacturer’s instructions.