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Citations for Millipore Sigma :
5451 - 5500 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... IL-17A and TNFα were quantified in culture supernatants using the mouse-specific Milliplex® multi-analyte panel kit MT17MAG-47K (Millipore; Sigma) and the MagPix® instrument platform with related xPONENT® software (Luminex Corporation) ...
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bioRxiv - Neuroscience 2020Quote: ... Both the antibodies targeted against Imp-β1 and puromycin were conjugated directly to the PLA probes as PLAplus and PLAminus respectively using Duolink™ In Situ Probemaker PLUS and MINUS kits (Sigma). Neurons were then fixed with 3.2 % PFA before processing with immunocytochemistry.
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as previously described (41,44) ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as described previously (Rumi et al. ...
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bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...
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bioRxiv - Microbiology 2020Quote: ... Peroxidase-conjugated secondary antibodies (Antgene, 1: 5000) were applied accordingly followed by image development with Chemiluminescent HRP Substrate Kit (Millipore Corporation).
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bioRxiv - Microbiology 2020Quote: Nuclear extracts (using NE-PER™ nuclear Extraction Kit (Thermo Fischer Scientific, USA) and whole-cell lysates (using RIPA buffer, Sigma) were prepared from MTB-infected macrophages (MOI 20:1) ...
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bioRxiv - Microbiology 2019Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: Interactions between the SRB1 and NS1 proteins from DENV and ZIKV in Huh-7 and C6/36 cells were detected using the commercial kit Duolink (Sigma-Aldrich) based on in situ proximity ligation assay (PLA ...
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bioRxiv - Microbiology 2020Quote: GlgE activity was monitored quantitatively in the elongation direction by the release of orthophosphate using the Malachite Green Assay Kit (Sigma-Aldrich) following the manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: ... post-fixed with 1% osmium tetroxide and mordanted with 1% tannic acid followed by dehydration through an ethanol series (contrasting with uranyl acetate at the 30% stage) and embedding with an Epoxy Resin Kit (all from Sigma-Aldrich). Semi-thin 0.5 μm sections were cut and collected onto clean glass slides and dried at 60°C before staining with 1% Toluidine Blue and 1% Borax (all from Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... and cloned into the mid-copy T7-FNS2 phage display vector as described previously,17 and packaged using the T7 Select Packaging Kit (EMD Millipore). Library quality assessment was performed as described in Results.
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bioRxiv - Microbiology 2020Quote: Phosphatase and kinase activities of SiaA and/or SiaB on SiaC and/or SiaCp were determined using the Malachite green phosphate assay kit (Sigma Aldrich) and ADP-Glo™ assay kit (Promega ...
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Treatment history shapes the evolution of complex carbapenem-resistant phenotypes in Klebsiella spp.bioRxiv - Microbiology 2022Quote: Genomic DNA of the Klebsiella isolates was prepared from solid media scrapings of pure culture using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) and the Gram-negative bacteria protocol ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Neuroscience 2022Quote: ... The paraffin sections were assayed for apoptotic cells using the ApopTag Plus Fluorescein in Situ Apoptosis Detection Kit (Millipore, Cat. #S7111) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 100-150 mg of snap-frozen tissue were mechanically homogenized in 750 µL of Lysis Solution/2-mercaptoethanol mixture and total RNA was isolated using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich). Reverse transcription for up to 2 µg of total RNA was performed using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Medium was refreshed 3 days after seeding and 3 days later cells were fixed with citrate/acetone/3% formaldehyde and stained with Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines including stable clones were routinely evaluated for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... Genotypes were verified by PCR of tail DNA using the RedExtract-N-Amp Tissue PCR Kit (Sigma Aldrich 254-457-8).
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments were analyzed on a 1.2 % agarose-gel and extracted with Kit Montage Gel Extraction columns (Millipore, Bedford, MA). The purified amplicons were used in a BP-reaction for the creation of Gateway-Entry vectors using 1 μL purified amplicon ...
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Necrosis reduction efficacy of subdermal biomaterial mediated oxygen delivery in ischemic skin flapsbioRxiv - Bioengineering 2022Quote: ... sections of the flap were frozen at - 80°C and processed [24] to measure the lactate content of the tissues using a Lactate assay kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2022Quote: NAD total levels in MT-4 cells and MT-4 cells infected by HIV-1 were assessed using the NAD/NADH Quantification Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Activity assays to see the inhibitory effect of Halo Library fragment binders on RT52A activity were performed using the Roche colorimetric assay kit purchased from Sigma Aldrich, using the absorbance measurement of 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid ...
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bioRxiv - Cell Biology 2022Quote: Fresh-frozen sections of young and aged bladders were tested for acid phosphatase activity using an acid phosphatase assay kit (CS0740-1KT, Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Mice were genotyped by PCR using ear biopsies collected within 4 weeks of birth and genomic DNA was extracted using Extract-N-Amp tissue prep kit (Sigma-Aldrich). Embryos were genotyped using either immune-reactivity to antibody raised against either STAT3 pY705 or TFCP2L1 in the case of those imaged for confocal analysis ...
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bioRxiv - Cell Biology 2022Quote: The assay was performed using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (# C10428, Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated by phenol chloroform extraction and alcohol precipitation followed by two consecutive rounds of poly-A selection using oligo(dT) beads (GenElute mRNA Miniprep Kit, Sigma MRN10) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm lengths of leaf tip) using the ‘Crude Preparation of Nuclei Procedures’ protocol of the CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich). DNA extraction from crude nuclei was performed using two different methods ...
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bioRxiv - Microbiology 2022Quote: ... ∼2×107 parasites were collected by centrifugation (1,200 x g for 10 min) and pull-downs were performed using the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Sigma-Aldrich). Unenriched ‘input’ samples were generated by reserving back 10% of the starting lysate ...
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bioRxiv - Microbiology 2022Quote: ... while spotting them onto a polished stainless steel MALDI target in a 1:3 ratio (0.5 μL sample/1.5 μL matrix) with addition of 0.25 μL ProteoMass Normal Mass Calibration Mix (1:10 dilution, ProteoMass™ MALDI Calibration Kit, Sigma-Aldrich) for acquisition of high resolution spectra ...
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bioRxiv - Microbiology 2022Quote: The activity of the MtPAPP was determined by the production of orthophosphate which was quantified using the Malachite Green Phosphate Assay Kit (Sigma-Aldrich) by forming a green complex ...
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bioRxiv - Microbiology 2022Quote: ... 12 and 16 hours of bacterium growth for 24 hours at 37°C with shaking (180 rpm) using the Iron Assay Kit (Sigma-Aldrich). TECAN infinite M200PRO microplate reader was used for measuring Colorimetric readings.
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bioRxiv - Pathology 2022Quote: ... One ug total RNA was used for first strand cDNA synthesis of all samples Transcriptor High Fidelity cDNA kits (Sigma Aldrich) was used for first strand cDNA synthesis of all samples ...
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bioRxiv - Pathology 2022Quote: ... Leaf tissues were ground and homogenized in liquid nitrogen for subsequent total RNA isolation with the SpectrumTM Plant Total RNA Isolation Kit (Sigma, USA), followed by on-column DNase I digestion treatment (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ATP levels in media from hydroponic infection experiments of hydroponically-grown plants were analyzed using the ATP Colorimetric/Fluorometric Assay Kit (Sigma, USA) and an Infinite M1000 plate reader (Tecan ...
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bioRxiv - Plant Biology 2023Quote: ... The 100 frozen leaves for each of these sample and the positive sample were extracted in pools of 25 following the Spectrum plant total RNA kit (Sigma-Aldrich). RNA extracts were tested by RT-PCR using the Titan One Tube RT-PCR kit (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... RNA from the original 100 frozen leaves of the positive sample were re-extracted in pools of 25 with the Spectrum plant total RNA kit (Sigma-Aldrich) and tested by RT-PCR using the Titan One Tube RT-PCR kit ...
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bioRxiv - Microbiology 2023Quote: ... The interactions between the two labeled proteins were detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (#DUO92101-1kit, Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Following primary antibody incubation coverslips were washed 2× in PBS and then the proximity ligation assay was carried out (Duolink In Situ Red Starter kit (Sigma Aldrich) according to the manufacturer’s instructions) ...
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bioRxiv - Neuroscience 2023Quote: ... and fed with StemFlex media (ThemoFisher Scientific) and undergo monthly mycoplasma testing to ensure cultures are mycoplasma-free with the LookOut Mycoplasma PCR detection kit (Millipore Sigma). For the differentiation of iPSC-derived microglia-like cells ...
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bioRxiv - Microbiology 2022Quote: DNA extraction was performed for each of the above isolated samples by following manufacturer’s instructions using the GenElute™ Bacterial Genomic DNA kit (Sigma-Aldrich). After gDNA extraction ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines tested negative for mycoplasma contamination using a PCR-based method using the VenorGeM Classic Mycoplasma Detection Kit (Sigma-Aldrich). STR-genotyping was performed to confirm the identity of HeLa-JVM ...
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bioRxiv - Molecular Biology 2022Quote: ... The tRNA-Pro probe sequence was amplified from this plasmid using the primers in Table S2 and in vitro transcribed by using Digoxigenin-11-UTP included in DIG Northern Starter Kit (Sigma, #12039672910). 25 µg total RNA each from WT and rad6Δ cells was resolved on 15% TBE-Urea polyacrylamide gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 x 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... which was then washed with up to 2.5 ml of digestion buffer (Tumour dissociation kit, Miltenyi, 130-096-730) plus Deoxyribonuclease I (300 μg/ml, Sigma, DN25-1G). Dissociation was performed using the protocol suggested by Miltenyi ...
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bioRxiv - Microbiology 2022Quote: ... total genomic DNA from each clone was extracted from 2mL of an overnight culture using the GenElute Bacterial Genomic DNA kit (Sigma-Aldrich) and the quality and integrity of the DNA was assessed on a 1% agarose gel and the concentrations were determined fluorometrically using Quant-iT™ PicoGreen® dsDNA Reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... mLGR5-eGFP or cLGR5-eGFP were used as target cells in the killing assay and were pre-loaded with CellVue membrane dye (CellVue Claret Far Red Fluorescent Cell Linker Kit protocol; Sigma-Aldrich) and 2.5 × 106 or 6.25 × 105 NK92 cells or CAR expressing NK92 cells were used at indicated effector to target ratios ...
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bioRxiv - Cell Biology 2022Quote: ... equal number of DB-VOs and control ND-VOs were labelled by LuminiCell Tracker 670-Cell Labeling Kit (SCT011, Sigma-Aldrich), 0.8nm for 12 hours in Stempro34 complete medium ...
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bioRxiv - Plant Biology 2024Quote: RNA of aerial and subterranean fruit valves (stage 16-17a and 17ab) was extracted from three biological replicates using SpectrumTM Plant Total RNA kit (Sigma-Aldrich). For long read sequencing ...