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Citations for Millipore Sigma :
5801 - 5850 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... and non-esterified fatty acids were measured with a commercial colorimetric kit according to manufacturer’s instructions as follows: pyruvate (Sigma Aldrich, MAK071, 10μL), amino acids (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was transferred to a fresh tube and total RNA was prepared using a GenElute total RNA purification Maxi kit (Sigma-Aldrich; RNB200) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis assays were performed in duplicate by detection of caspase-3 activity using a fluorometric [µM AMC] (or/colorimetric [µM PNA/min/ml] unless stated) assay kit (Cat#APPA015-1KT/CASP3C-1KT, Sigma, London, England) according to the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2019Quote: ... SAF-A:RNA complexes were detected in BrU-labeled cell populations using the rabbit α-SAF-A and mouse α-BrU antibodies in conjunction with the Duolink Proximity Ligation Assay kit (Sigma, DUO92102).
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bioRxiv - Cell Biology 2020Quote: ... PLA probes were applied in 1:10 dilution using the diluent buffer provided in the Duolink In Situ Red kit (Sigma Aldrich, cat# DUO92101). Incubations and subsequent ligation and amplification steps were performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... in the case of young oothecae (from NFE to 4-day-old) and GenElute Mammalian Total RNA Miniprep kit (Sigma-Aldrich, Madrid, Spain) in the case of older oothecae (from 6- to 16-day-old) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 15 mg of the tissue from each mouse liver was used for genomic DNA extraction using GenElute Mammalian Genomic DNA Miniprep Kit (Millipore Sigma Cat. #: G1N350).
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bioRxiv - Cell Biology 2022Quote: ... were grown in ATCC’s vascular cell basal medium supplemented with microvascular endothelial cell growth kit-VEGF and 12.5 μg/mL blasticidine (Sigma-Aldrich, St Louis, MO) for 48 h at 37°C + 5% CO2 until reaching 80-90% confluence ...
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bioRxiv - Genomics 2020Quote: ... The lysates were then probed for phosphorylated STATs using the Milliplex map STAT cell signaling magnetic bead 5-Plex kit (Millipore Sigma, 48-610MAG) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... 5-10 μg protein lysates were cultured and proteasome activity measured using the 20S Proteasome Activity Assay kit (APT280, Millipore, Billerica, MA, USA) per manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... DNA was prepared from a 3 ml culture of the representative strain using the GenElute ™ Bacterial Genomic DNA Kit (Sigma Aldrich, Sweden) [7].
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bioRxiv - Physiology 2019Quote: ... leptin and insulin were analyzed by commercial ELISA’s according to manufacturer’s instructions (EIA CORT kit, Arbor Assays, Michigan, USA; adiponectin, insulin and leptin, Milliplex Mouse Adipokine Multiplex, Millipore, Amsterdam, The Netherlands).
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bioRxiv - Cell Biology 2019Quote: ... PLA probes were applied in 1:10 dilution using the diluent buffer provided in the Duolink In Situ Red kit (Sigma Aldrich, cat# DUO92101). Incubations and subsequent ligation and amplification steps were performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... For ChIP-qPCR 10 µl of IP samples were amplified using the Genomeplex Single Cell Whole Genome Amplification Kit (WGA4) (Sigma Aldrich, Taufkirchen, Germany) and subjected to qPCR in a 1:40 dilution ...
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bioRxiv - Developmental Biology 2019Quote: TUNEL assay to label apoptotic and dead cells was performed using the ApopTag® Red In Situ Apoptosis Detection Kit (Millipore, cat. #S7165) following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Neuroscience 2019Quote: ... and visualized by exposing the membrane to a charge-coupled device camera (LAS1000, Fujifilm, Tokyo, Japan) using a chemiluminescence kit (Merck Millipore, Billerica, MA, USA). Membranes were stripped and reprobed for β-actin (diluted 1:50 000 ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA from ~100 mg of powdered tissue that was kept at −80 °C was extracted using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and treated with DNAse I (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: In situ Proximity Ligation Assay (PLA) was performed with Duolink in situ Red Starter kit Mouse/Rabbit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The sheared chromatin was then incubated with anti-ARID1A antibody and purified by using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore, 17-10086). Chromatin DNAs quality was assessed by 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Serial sections for trabecular bone were obtained from the distal femur at a thickness of 5 µm followed by tartrate-resistant acid phosphatase (TRAP) staining or alkaline phosphatase (ALP) staining using TRAP kits (Fast Red TR/Naphthol AS-MX, Sigma, St. Louis, MO) and NBT/BCIP (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... donor cells were labelled with CellVue Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labelling (Sigma Aldrich, Cat#MINCLARET-1KT) following manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Both cell lines were maintained in a 5% CO2 humidified incubator at 37°C and monitored periodically for mycoplasma contamination (Sigma Venor GeM Kit). Unless otherwise indicated ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was isolated using the Gene Elute Mammalian Total RNA Miniprep Kit according to the manufacturer’s protocol (Sigma–Aldrich, St. Louis, MO, USA). Concentration of the isolated RNA was determined photometrically with a BioSpectrometer® (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... were authenticated by short tandem repeat (STR) profiling and tested for mycoplasma contamination prior to experiments using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MP0035-1KT). HEK293T (ATCC CRL-3216 ...
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bioRxiv - Molecular Biology 2020Quote: ... Densitometry was performed as previous described.19 Proximity ligations between ephrinb2/EphB4 in flow-aligned cells were conducted by using Duolink® In situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich, MO). Numbers of individual ligations in raw and post-processed images were compared to test the validity of the quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... glucose metabolic inhibitor 2DG (10 mM or 20 mM) was added 15 min before cell lysis and ATP determination using the ATP Bioluminescence Assay Kit HS II (Sigma catalogue number 11699709001) and analyzed on an Infinite M200 PRO Luminometer (Tecan).
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bioRxiv - Molecular Biology 2022Quote: Cell were fixed/permeabilized with BD Cytofix/Cytoperm™ Kit (Cat 554714) and stained with DAPI (4,6, diamidino-2-phenylindole, Sigma-Aldrich, Cat D9542). DAPI (1 in 100 dilution ...
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bioRxiv - Microbiology 2022Quote: ... the entire surface of the agar plate was scraped using a loop and deposited into 180 μl of lysis solution provided by the GenElute Bacterial Genomic DNA extraction kit (Sigma-Aldrich, Darmstadt, Germany). For approach 2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These products were purified by running on a 1% agarose gel and gel extracted using the GenElute™Gel Extraction kit (Sigma-Aldrich, USA). The extracted bands were Sanger sequenced by Eurofins (USA ...
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bioRxiv - Plant Biology 2022Quote: ... Full-length FIN4 mRNA in PCRII-TOPO vector was used to synthesize sense and anti-sense probes using a DIG-labeled probe synthesizing kit (Millipore-Sigma, catalog number 11175025910) with T7 RNA polymerase for sense probe and SP6 RNA polymerase for antisense probe synthesis with 1μg plasmid as template ...
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bioRxiv - Microbiology 2019Quote: ... Each viral DNA sample was then amplified using GenomePlex® Complete Whole Genome Amplification (WGA2) Kit from Sigma-Aldrich (Gudenkauf and Hewson, 2016). Two blank controls were included in this step ...
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bioRxiv - Immunology 2019Quote: Chitinase activity was analyzed by using the substrate and standard solutions of the fluorimetric chitinase assay kit (Sigma-Aldrich Chemie GmbH, Munich, Germany). Substrates 4-MU-(GlcNAc)2 (Exo-Chitinase activity ...
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bioRxiv - Systems Biology 2019Quote: ... The assay named ‘Other Luminex’ was performed only for study SLVP015 in 2007 using the Human 42-Plex Polystyrene Kit (EMD Millipore, H42; MPXHCYTO060KPMX42) and data was processed in the same way as for the Luminex assays described above (measurement units reported were Zlog2)28.
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bioRxiv - Developmental Biology 2019Quote: Apoptosis induced DNA damage was measured using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit (cat no. S7100, EMD Millipore, Temecula, CA) following the manufacturer’s protocol for paraffin-embedded tissue ...
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bioRxiv - Pathology 2020Quote: Total RNA was extracted from four leaf discs per replicate (three replicates, each sampled from two independent flood-inoculated ‘Hort16A’ plantlets) via a Spectrum™ Plant Total RNA kit (Sigma Aldrich, NZ). RNA was DNase I (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Hybridization and all other procedures followed the manufacturer’s instructions using the DIG High Prime DNA Labeling and Detection Starter Kit-I (Sigma Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2020Quote: Larvae were processed as described (121) using the DuoLink Mouse Rabbit in situ PLA Kit (Sigma-Aldrich, cat. no. DUO92101, St. Louis, MO). Controls were performed with one or the other epitope-tagged transgene absent or with the probes replaced by water during the first PLA step to ensure that signal observed as not background or bleed-through of channels ...
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bioRxiv - Pathology 2021Quote: All samples were stained at the same time under the same conditions using a modified Fontana-Masson Kit staining protocol (Sigma-Aldrich Procedure #HT200). Prior to staining ...
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bioRxiv - Microbiology 2020Quote: ... 200ml of culture grown to stationary phase was concentrated using centrifugation (27,000 × g for 15 minutes) and extracted with the GenElute Bacterial Genomic DNA Kit (Millipore Sigma, Burlington, MA, USA). Basecalling was done using Guppy version 2.2.3 and long reads were filtered using filtLong version 0.2.0 (https://github.com/rrwick/Filtlong) ...
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bioRxiv - Genetics 2019Quote: ... Total RNA for both root and shoot tissues was extracted using the Spectrum™ Plant Total RNA Kit (Sigma-Alridch, Carlsbad, California, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was discarded and supernatant was used to analyze the GST activity (Thounaojam et al. 2012). GST activity was performed according to method (Habig et al. 1974) by utilizing GST Assay kit (Sigma, USA, CS0410-1KT)
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bioRxiv - Microbiology 2020Quote: ... Pellets were processed according to the B protocol of the NXTRACT kit (CelLytic™ NuCLEAR™ Sigma-Aldrich, Milan, Italy https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/nxtractbul.pdf), in order to obtain native nuclear proteins ...
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bioRxiv - Biochemistry 2020Quote: ... concentrations in the different culture media were determined by the Bio-Plex method using the mouse adipocyte magnetic bead panel kit (Millipore, MADCYMAG-72K-07), according to manufacturer’s instructions (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then clarified by centrifugation and the protein content of each supernatant was determined using the Quanti-Pro bicinchoninic acid (BCA) assay kit (Sigma-Aldrich, MO, USA). Equal protein amounts (100 μg ...
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bioRxiv - Biochemistry 2021Quote: The ATPase activity of wild-type LonP1 and its mutants was measured with ATPase/GTPase activity assay kit (Sigma-Aldrich, USA, product: MAK113) as per the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: Sample preparation - In-gel digests were performed for each excised sample using the ProteoExtract All-in-One Trypsin Digestion Kit (Cat. No. 650212; Calbiochem, EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Masson’s trichrome Staining was applied according to the manufacturer’s instructions of the Trichrome Stain (Masson) Kit (HT15-1KT, Sigma-Aldrich, Heatherhouse, United Kingdom). For Safranin O/Fast green staining ...
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bioRxiv - Cancer Biology 2021Quote: ... EdU detection reagents were then added for 2 hours at room temperature in the dark (Click-iT™ Assay Kit, Sigma-Aldrich #C10337). Nutlin was used as a positive control (Selleckchem #S7101) ...