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Citations for Millipore Sigma :
5601 - 5650 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... To quantify the concentration of purified protein in the samples a Bicinchoninic acid (BCA) assay was conducted using the BCA kit following the manufacturer recommendations (Sigma Aldrich).
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bioRxiv - Microbiology 2024Quote: ... The absence of mycoplasma contamination in the virus stocks was confirmed using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma; #MP0035).
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in VitroGel at 50% confluence on chamber slides for PLA (Proximity Ligation Assay) using the Duolink in situ Detection Reagents Red kit (DUO92008, Sigma-Aldrich), following the given protocol ...
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bioRxiv - Cell Biology 2024Quote: ... was performed to detect the interactions between ITGβ1 and CD44 using Duolink® In Situ Red Starter Kit Mouse/Rabbit (DUO92101, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Cytotoxicity was studied according to the manufacturer’s instruction either using cytotoxicity LDH assay kit-WST (Dojindo) or by measuring transepithelial electrical resistance (TEER) (Millicell® ERS-2, Millipore) as previously described (25) ...
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bioRxiv - Developmental Biology 2024Quote: ... immune cell samples were first heat inactivated at 70°C for 10 mins and then subjected to metabolite analysis using GOD-POD kit (Sigma#GAGO20) and Triglyceride assay kit (Sigma#T2449 ...
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bioRxiv - Immunology 2024Quote: Luminex assays detecting soluble ICAM-1 (sICAM1) were conducted using the MILLIPLEX Human Sepsis Magnetic Bead Panel 1 Kit (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Unbound dye was removed using dialysis against a 1000-fold excess of PBS or Broccoli aptamer buffer at 4 °C overnight with the Pur-A-LyzerTM Mini 6000 Dialysis Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... Recovered adherent cells were resuspended in MSC medium after centrifugation and counted using the GuavaEasycyte 8HT apparatus in Muse Count & viability kit (MCH600103, Cytek Biosciences) or with trypan blue (T8154, Sigma-Aldrich). Part of cells were frozen at 1×106 cells/vial in FBS containing 10% DMSO ...
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bioRxiv - Genetics 2024Quote: ... The ADP/ATP ratio was quantitatively measured from lysis supernatant using the Sigma ADP/ATP Assay Kit (Sigma-Aldrich, MAK135-1KT), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the supernatant was diluted and subjected to H3K9me3 ChIP using the ChIPAb+ Trimethyl-Histone H3 (Lys9) kit (Millipore, cat# 17-10242). Bound material was recovered after incubation with 15 μL of protein G beads (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed in tNCC or SHEPMYCN cells after 24 h Dox induced overexpression of MYCN using a Duolink PLA kit (Sigma, DUO92014) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... or ear biopsies (3-week-old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Neuroscience 2024Quote: ... digoxygenin (DIG)-labeled antisense probes were prepared using the Roche DIG labeling kit (Sigma Cat # 11277073910 and T7 (Sigma Cat # 10881767001) or T3 (Sigma Cat # 11031163001 ...
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bioRxiv - Neuroscience 2024Quote: ... “Indirect” PLAs for nitrated hASYN and nitrated NDUFB8 were performed using probes already conjugation to secondary antibodies and provided in a PLA kit (Duolink, Sigma-Aldrich). Primary antibodies used for indirect PLA were ...
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bioRxiv - Bioengineering 2024Quote: ... the expression of early differentiation marker (alkaline phosphatase, ALP) and late-stage differentiation marker (calcium mineralization) were quantified using ALP staining kit (Sigma Aldrich) and Alizarin Red S (ARS ...
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bioRxiv - Cell Biology 2024Quote: ... β-galactosidase (X-gal) lung staining was performed on frozen lung sections using the Senescence Cells Histochemical Staining Kit (Sigma CS0030) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: The interaction between PRDX6 and SEPHS2 in SK-N-DZ WT and SK-N-DZ PRDX6 KO cells was observed using Duolink in situ proximity ligation assay (PLA) kit (DUO92101, Sigma- Aldrich) in the presence or absence of 600nm Selenocysteine ...
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bioRxiv - Developmental Biology 2024Quote: ... and pCMV-Sport6.ccdb-marcksl1.L were linearized with EcoRV followed by synthesis of anti-sense RNA probes using the digoxigenin RNA Labeling Kit (Sigma Aldrich) and SP6 polymerase ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in 20 mL deionized water and dialyzed using the Pur-A-Lyzer Mega 3500 Dialysis Kit (Sigma-Aldrich) and lyophilized ...
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bioRxiv - Cell Biology 2020Quote: Chromatin from D5 control MEFs or D7 reprogrammed MEFs was isolated using the Magna ChIP A/G Chromatin Immunoprecipitation kit (Millipore 17-10085) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and RT-qPCR was performed as described previously [48] using QuantiTect SYBR Green PCR kit and KiCqStart SYBR Green optimized primers from Sigma-Aldrich (KSPQ12012).
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bioRxiv - Cell Biology 2019Quote: ... to quantify protein vicinity in HeLa cells and in neurons on coverslips as indicated in figures were performed using the Duolink In Situ Detection Reagents Orange kit (Sigma-Aldrich, DUO92007). Cells were fixed ...
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bioRxiv - Immunology 2021Quote: ... The proteins were resuspended and passed through EndoTrap® red endotoxin removal kit (LIONEX GmbH, Germany) and stored in endotoxin free water (Cat. No.#95289-1L, Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... Cy3-STxB was generated by conjugating Cy3 dye onto STxB according to the manufacture manual of CY®3 Ab Kit (PA330000, Sigma-Aldrich).
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bioRxiv - Genomics 2021Quote: ... Alkaline phosphatase testing for the assessment of pluripotency before and after auxin treatment was performed using the Alkaline Phosphatase Detection Kit (EMD Millipore, SCR004).
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bioRxiv - Microbiology 2021Quote: ... Serum cytokine profiling targeting 22 cytokines/chemokines was performed by Public Health England using a 22 -plex customized Luminex kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2020Quote: Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from each three biological replicates of 100 mg pollen grains/pollen tubes by using a Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Cancer Biology 2022Quote: For RNA-binding protein immunoprecipitation (RIP) ~3×107 A549 cells for each sample were processed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was used to assay for ANP levels using a competitive enzyme Immunoassay kit from Sigma-Aldrich (Catalog Number RAB0385). The levels of ANP were normalized for the amount of protein ...
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bioRxiv - Molecular Biology 2020Quote: The ATPase activity assay was performed in 96-well microtitre plates by using an ATPase/GTPase activity assay kit (Sigma-Aldrich, USA) according to the manufacturer’s instructions ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... plants grown under a long day photoperiod (16 h light/8 h dark, 60% humidity) using the SpectrumTM Total RNA extraction kit (Sigma-Aldrich, USA). The sample was enriched for mRNA using the Poly(A ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... Colonies were picked and grown in 5 ml liquid SD-L-W medium and the prey vectors were isolated using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, USA) after cell wall disruption ...
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bioRxiv - Cell Biology 2022Quote: The in-situ interaction of NICD and active β-catenin was determined using the Duolink™ II secondary antibodies and detection kits (Sigma–Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Colorimetric assay to measure alanine aminotransferase activity in the cell lines was performed using the ALT activity assay kit from Sigma (MAK052-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Genomics 2020Quote: ... Chromatin was prepared using the MAGnify Low Cell ChIP-Seq Kit (Thermo Fischer) and ChIP was done for H3K4me3 (Millipore, # 07-473), H3K27ac (Abcam ...
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bioRxiv - Neuroscience 2019Quote: Individual wells from cultured cells and tissue from mice was collected and then the homogenate was fractionated utilizing a NXTRACT CelLytic™ NuCLEAR™ Extraction Kit (Sigma) according to the manufacturer’s instructions ...
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Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... TUNEL assay was performed on 20 μm thick cryosections using ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, Cat. # S7100) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2020Quote: DIG-labeled riboprobes were generated using 2ul of PCR template with the Roche DIG RNA Labeling Kit (T7) (Sigma aldrich, SKU 11277073910)
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bioRxiv - Microbiology 2019Quote: ... Positive bacteria clones were inoculated into 50ml LB/carbnecillin (100 μg/ml) culture for amplification and midiprep plasmid purification using GenElute™ HP Plasmid Midiprep Kit (NA0200, Millipore Sigma). All plasmids except T3D-L1 were amplified at 37°C for 14-16hrs ...
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bioRxiv - Pathology 2019Quote: ... Sections were stained for tartrate-resistant acid phosphatase (TRAP) to examine osteoclast activity and numbers (Sigma-Aldrich Kit, St. Louis, MO, USA), and Fast Green counterstaining and examined with a Nikon Eclipse 90i Advanced Research Scope ...
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bioRxiv - Biochemistry 2019Quote: The detection of apoptotic cells was based on the nick-end (TUNEL) labelling method using the ApopTag® in situ detection kit (S7100, Merck Millipore, USA), according to the manufacturing protocol.
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bioRxiv - Genetics 2019Quote: Quantitative amplification was performed according to manufacturer’s specifications using KAPA SYBR® FAST qPCR Kit Master Mix (2X) Universal (Sigma, cat. #07959397001). 12 separate RNA isolations were analyzed ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... R&D Systems cytokine kits (Minneapolis, MN) or a high throughput multiplex cytokine assay system (Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel, Millipore, MO, USA) was used for cytokine quantification ...
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bioRxiv - Cancer Biology 2019Quote: ... TUNEL staining was performed on deparaffinized sections to detect late-stage apoptotic cells using the ApopTag peroxidase in situ apoptosis detection kit (Millipore, Burlington, MA), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using an ApopTag® In Situ Peroxidase Detection Kit (S7100, Merck Millipore, USA) according to the manufacturer’s recommendations and as previously described (36) ...