Labshake search
Citations for Millipore Sigma :
5751 - 5800 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... Cell lines were kept at 37°C in 5% CO2 and routinely checked for mycoplasma contamination via LookOut Mycoplasma PCR detection kit (Sigma, Cat. MP0035).
-
bioRxiv - Cancer Biology 2023Quote: ... a polymerase chain reaction (PCR)-based method was used to screen for mycoplasma once a month employing the LookOut® Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) and JumpStart™ Taq DNA Polymerase (D9307 ...
-
bioRxiv - Biochemistry 2024Quote: The concentration of both activated proteins—PA-BFP and SumireF—was determined by the bicinchoninic acid assay using the QuantiPro™ Kit (Sigma–Aldrich), following the supplier’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... and potential positive clones were grown overnight in LB media for plasmid production and further purification with GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). After confirmation of the insert sequence by sequencing (DNA Sequencing Unit ...
-
bioRxiv - Cancer Biology 2024Quote: MDA-MB-231-derived sEVs were labelled using the PKH67 Green Fluorescent Cell Linker kit (for general cell membrane labelling, Sigma-Aldrich, USA) according to manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2022Quote: ... samples were obtained from the tissues indicated in the text and RNA was isolated using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich). Total RNA (1 μg ...
-
bioRxiv - Cell Biology 2023Quote: ... Liver function was assessed by measuring the concentration of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum by using an ALT Activity Assay Kit (Sigma- Aldrich, MAK052) and an AST Activity Assay Kit (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: Cytokines from mouse brain homogenates prepared in section 2.7 were measured using Millipore mouse cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
-
bioRxiv - Microbiology 2023Quote: ... ATP in the supernatant was determined with an ATP bioluminescent assay kit according to instructions from the manufacturer (Sigma-Aldrich, FLAA-1KT).
-
The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in the Antibody Diluent Reagent from the kit [mouse anti-c-myc (clone 9E10; 1:200, Sigma-Aldrich #11667149001) and rabbit anti-HA (1:200 ...
-
bioRxiv - Immunology 2023Quote: ... The first kit used was the Milliplex® SARS-CoV-2 antigen panel 1 IgA assay (Millipore Sigma, Cat No. HC19SERA1-85K) with the Wuhan-1 strain RBD and N proteins included ...
-
bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
-
bioRxiv - Pathology 2023Quote: The SA-βgal activity was assessed in RPE cells at the end of the experiment using the Senescence Detection Kit (Sigma-Aldrich, #CS0030) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... ProteoExtract® native membrane protein extraction kit and 10 kDa Amicon® Ultra-15 centrifugal filters were purchased from Millipore Sigma (USA). Assays for measuring cell toxicity were purchased from Promega (Madison ...
-
bioRxiv - Microbiology 2023Quote: ... All cell lines were tested every two weeks for mycoplasma contamination either by in-house DAPI staining or LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich).
-
bioRxiv - Genomics 2023Quote: ... Filtered nuclei suspension was pelleted as instructed and then resuspended in 50 ul Storage Buffer from the EZ nuclei kit (Sigma Aldrich, NUC101). For downstream processing ...
-
bioRxiv - Cell Biology 2023Quote: ... and Vap33 and Git in Drosophila larval tissues were detected in situ using the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma, DUO92101) according to the instructions of the manufacturer ...
-
bioRxiv - Cancer Biology 2023Quote: ... T cell migration potential across an endothelial barrier in vitro was assessed using a QCM™ Leukocyte Trans-endothelial Migration Colorimetric Assay kit (Cat: ECM557, Millipore Sigma) following the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2022Quote: ... and hearts), mouse organs (livers, brains, and hearts) and human hearts and brains was performed with a commercial kit (Sigma-Aldrich, 11814770001) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... The sonicated nuclear fractions were centrifuged at 10k xg at 4 ⁰C for 10 min and then chromatin immunoprecipitation (ChIP) assay was followed with manufacturers’ guidance for EZ-Magna Chip A/G kit (Millipore, #17-10086) with minor modification ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated from overnight cultures of the wild type and mutant strains using GenElute Bacterial Genomic DNA Kits (Sigma-Aldrich, USA). Library preparation and whole genome sequencing were performed by Glasgow Polyomics ...
-
bioRxiv - Neuroscience 2023Quote: ... the cells were homogenized and then fractionated to obtain plasma membrane and cytosolic fractions using the ProteoExtract Subcellular Proteome Extraction Kit (Millipore, Billerica, MA). Antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
-
bioRxiv - Genetics 2023Quote: ... blots were hybridized with Digoxigenin (DIG)-labeled DNA probes (Supplementary Table 2) amplified by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich; 11636090910). The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
-
bioRxiv - Genomics 2023Quote: ... The initial DNA amplification of the collected chromosomes was performed using a GenomePlex Single Cell Whole Genome Amplification Kit (WGA4) (Sigma-Aldrich, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were then dehydrated in a graded acetone series (30-100%) followed by resin embedding using an epoxy embedding kit (Fluka Analytical, Sigma Aldrich, Germany). Ultra-thin sections were obtained with a Leica EM UC6 (Leica Co ...
-
bioRxiv - Molecular Biology 2023Quote: ... −5 °C for 4 minutes using the ZentriMix 380R (Hettich) and RNA was purified using the GeneElute™ Mammalian Total RNA Midiprep Kit (Sigma Aldrich) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Chromatin immunoprecipitation assay was carried out to assess the functional status of p53 using Magna ChIP™ G Tissue Kit (Millipore, USA). Briefly ...
-
bioRxiv - Plant Biology 2023Quote: ... were processed to isolate RNA starting from at least 50 mg of lyophilized tissue using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) following manufacturer’s instructions (Nerva et al. ...
-
bioRxiv - Plant Biology 2023Quote: ... were grown to late-log phase in photoautotrophic standard conditions for total RNA extractions using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, STRN50) by following manufacturer instructions with the following modifications ...
-
bioRxiv - Plant Biology 2023Quote: Genomic DNA of founders and RILs was extracted bulking young leaves of three seedling per genotype with the GenElute Plant Genomic DNA Miniprep Kit (Sigma Aldrich, Germany) following the manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2023Quote: Caspase-9 activity was measured in liver lysate according to manufacturer’s protocol (Merck Caspase-9 Colorimetric Activity Assay Kit APT139, EMD Millipore Corporation, USA). In brief ...
-
bioRxiv - Physiology 2023Quote: Dissected fat bodies pooled from 10 3rd instar larvae were homogenized for glycogen and glucose measurements according to the Glucose assay kit (Sigma-Aldrich #GAGO20).
-
bioRxiv - Cell Biology 2023Quote: ... 50 mg of cryogenically pulverized lung tissue was homogenized in 500 μL ultrapure H2O and hydroxy proline content was measured using a Hydroxyproline Assay Kit (Sigma-Aldrich, MAK357) following manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ALT and AST activity and plasma AFP levels were determined in plasma with the commercially assay kits (Sigma-Aldrich, MAK052; and MAK055) following manufacturer’s recommendations.
-
bioRxiv - Genetics 2023Quote: ... Duolink probes (mouse plus and rabbit minus) were prepared and applied as described by manufacturer’s instructions for the Duolink® PLA kit Cy5 (Millipore-Sigma DUO92013).
-
bioRxiv - Cell Biology 2023Quote: ... Live-cell AIS staining was performed using a mouse anti-neurofascin antibody targeting an extracellular epitope (NeuroMab clone A12/18, #75-172) conjugated with CF647 (Mix-N-Stain CF647 antibody labeling kit, Sigma-Aldrich #MX647S50) following the manufacturer protocol ...
-
bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assays were performed in liver cryosections from frozen tissue using Duolink® In situ Red Starter Kit Mouse/Rabbit from Sigma-Aldrich, according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were blocked in 1% BSA (in PBS) for 1 hour at room temperature and processed for PLA using the DuoLink in situ Red Starter Kit (Millipore Sigma #DUO92101) per manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: We used T7 cysteine constrained heptapeptide (CX7C) phage libraries previously made in our lab(16, 18) using T7Select415-1 cloning kit (Novagen, catalog #70015) to select against CF pHBECs to discover mucus and cell-penetrating peptides ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Final concentration was measured by Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific. RNA integrity was assessed by gel electrophoresis with 1:1 formamide (Merck Millipore) and 1 µl of loading dye on 1.5% agarose gel ...
-
ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... viability measurements were performed for the 1 vpc and 3 vpc conditions of all vectors and two wells of uninfected cells with the Cell Counting Kit 8 (CCK8, Sigma-Aldrich #96992). Subsequently ...
-
bioRxiv - Molecular Biology 2023Quote: ... The aortas at the aortic valve level were stained using an Elastica van Gieson staining kit (Sigma-Aldrich, Inc., St. Louis, MO) and Picrosirius red staining kit (Polysciences ...
-
bioRxiv - Neuroscience 2024Quote: The cytokine concentrations of organoid lysate samples were quantified on the Luminex FLEXMAP3D platform using the Milliplex human cytokine and chemokine magnetic bead panel kit (Millipore HCYTOMAG-60K), measuring a broad panel of immune signaling proteins that allow for an unbiased survey of immune cues activating a diverse set of downstream intracellular pathways ...
-
bioRxiv - Developmental Biology 2024Quote: ... and sectioned on a microtome to obtain 5 µm sections which were then stained using a Masson Trichrome staining kit (Sigma Aldrich, HT15) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: Apoptosis assays were performed in duplicate by detection of caspase-3 activity using a fluorometric [µM AMC] (or/colorimetric [µM PNA/min/ml] unless stated) assay kit (Cat#APPA015-1KT/CASP3C-1KT, Sigma, London, England) according to the manufacturers’ protocols ...
-
bioRxiv - Neuroscience 2024Quote: All cell cultures were tested for mycoplasma contamination once per month using LookOut® Mycoplasma PCR Detection Kit (Sigma, cat.no. MP0035-1KT).
-
bioRxiv - Biochemistry 2024Quote: ... the supernatant was transferred to a fresh tube and total RNA was prepared using a GenElute total RNA purification Maxi kit (Sigma-Aldrich; RNB200) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... 0.4 µl 10 µM reverse tailed specific primer and 12.72 µl PCR grade water (contained in the Kap2G Robust PCR kit Sigma Catalogue No. KK5005) per sample and 18 µl added to each well to be used in a 96-well plate followed by 2 µl of DNA and mixed ...
-
bioRxiv - Microbiology 2024Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...