Labshake search
Citations for Millipore Sigma :
5851 - 5900 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: The peptide/ZO1 interaction was detected in situ using the Duolink secondary antibodies and detection kit (Sigma, St Louis, MO, Cat: 92002, 92004) according to manufacturer instructions ...
-
bioRxiv - Immunology 2021Quote: ... The pyrosequencing assay was validated using SssI-treated human genomic DNA as a 100% methylation control and human genomic DNA amplified by GenomePlex Complete Whole Genome Amplification kit (Sigma-Aldrich, WGA2-50RXN) as 0% methylation control ...
-
bioRxiv - Microbiology 2022Quote: ... The iron content of pre and post Chelex® 100 treatment was determined using the Iron Assay Kit (Sigma-Aldrich, MA, United States) following the manufacturer’s recommendations (Table S5).
-
bioRxiv - Cell Biology 2022Quote: ... Conditioned media was run on the MilliPLEX Human High Sensitivity T Cell Magnetic Bead Panel Luminex kit for measurement of 21 unique cytokines per plex according to manufacturer’s instructions (EMD Millipore, St. Louis, MO).
-
bioRxiv - Microbiology 2022Quote: ... Serum cytokine levels were measured by the Indiana University Multiplex Analysis Core using a Millipore Milliplex MAP Mouse Cytokine/Chemokine Magnetic Kit (Millipore Sigma, Burlington, MA). Undiluted serum was provided to the Indiana University Multiplex Analysis Core and ran according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: Gram staining of the LT0009 isolate was performed using a Gram-staining kit according to the manufacturer’s instruction (Sigma Aldrich, 77730-1KT-F) and its cellular morphology was imaged with a scanning electron microscope (JSM-IT300 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The senescence condition in the two cellular models was assessed by monitoring the morphological changes and by β-galactosidase (β-gal) staining with the Senescence Cells Histochemical Staining Kit (CS0030, Sigma-Aldrich). After reaching the preferred condition ...
-
bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
-
bioRxiv - Neuroscience 2023Quote: The TUNEL assay was performed on paraffin-embedded sections and fifty-micron-thick free-floating frozen sections according to the manufacturer’s instructions but with the time of some steps lengthened to ensure good penetration of the reagents into the tissue (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Millipore-Sigma, Cat # S7110). Sections were post-fixed for ten minutes at room temperature in 2:1 ethanol:acetic acid ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated in biotinylated anti-rabbit secondary antibody 1:500 (Vectors Laboratories, #BA-1000) followed by Avidin/Biotin complex amplification (Vectastain Elite ABC kit; Vectors Laboratories, #PK-6100) and DAB (Sigma Life Science, #5905) mixed with 3% hydrogen peroxide for signal amplification ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell lines were regularly checked for mycoplasma contamination using Venor™ GeM Mycoplasma Detection Kit (Sigma-Aldrich -Aldrich Co., Catalog no. MP0025-1KT).
-
bioRxiv - Bioengineering 2024Quote: ... Imaging signal was visualized using imaging kits (SuperSignal™ West Pico PLUS Chemiluminescent Substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate, Sigma-Aldrich) via an Image Quant LAS 4010 chemiluminescence imager (GE Healthcare) ...
-
bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted (from TNBC cell lines or cryopreserved tumor tissue) using the GenElute mammalian total RNA miniprep kit (Sigma/Merck, RTN350-1KT) according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck ...
-
bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
-
bioRxiv - Genomics 2024Quote: ... Medium containing supplements were re-fed every 3-4 days for a total of 12 days after which the cells were evaluated for morphological changes and stained for tartrate-resistant acid phosphatase (TRAP) with a commercially available leukocyte acid phosphatase kit (SIGMA, Cat. 387-A). Three replicates of differentiated cells were processed to prepare samples for ATAC-seq at 0 ...
-
bioRxiv - Synthetic Biology 2023Quote: Measurements of phosphate concentration in the growth medium supernatant were done using a Malachite green phosphate assay kit (Sigma-Aldrich, Cat. No. MAK307). After collecting the supernatant ...
-
bioRxiv - Immunology 2023Quote: Cells from day 5 bone marrow cultures from tet-LIN28B or littermate control mice were used for lysis and RIP using Magna RIP RNA-Binding Protein Immunoprecipitation kit (Millipore, cat#17-700) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... All bacterial and eukaryotic cell stocks were confirmed to be Mycoplasma negative using the LookOut Mycoplasma PCR Detection Kit (Sigma, St. Louis, MO). Molecular biology reagents were purchased from ThermoFisher unless otherwise noted.
-
bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from 30–100 mg of ground (with liquid nitrogen) petal tissues using the Tri-Reagent Kit (Sigma-Aldrich, https://www.sigmaaldrich.com) and treated with RNase-free DNase I (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2023Quote: Chitinase activity in the culture supernatant of CHIT1 plasmid-transfected HEK293T cells was measured by using a fluorescence assay kit (Sigma-Aldrich Cat. # CS1030). A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate ...
-
bioRxiv - Neuroscience 2023Quote: ... leptin and insulin levels were measured in 12 h-fasted mice using a Mouse Metabolic Hormone Magnetic Bead-based immunoassay kits (Millipore Corp, Billerica, MA) according to the manufacturer instructions on samples obtained at the sacrifice.
-
bioRxiv - Pathology 2023Quote: Multicolor in situ PLA was performed using the Duolink PLA Multicolor detection kit (Duolink® PLA® Multicolor Reagent Pack, DUO96000, Sigma-Aldrich) as described previously(58 ...
-
bioRxiv - Plant Biology 2023Quote: Nuclei were extracted from flash-frozen leaf tissue using a Sigma CelLytic™ PN Plant Nuclei Isolation/Extraction Kit (Sigma-Aldrich, Burlington, Massachusetts). Crosslinking was performed following low-input protocols from Arima Genomics (Arima Genomics ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell toxicity was measured in 96-well plates (∼5×104 cells/well) using a lactate dehydrogenase (LDH) cytotoxicity kit (Sigma-Aldrich, Cat # 11644793001). Absorbance was measured at λ=490 nm ...
-
bioRxiv - Immunology 2023Quote: Concentrations of cytokines in supernatants of T cell cultures were assessed with the MILLIPLEX MAP Human Th17 magnetic bead panel kit (Merck Millipore, Burlington, MA, USA) and the Luminex® 200™ system according to the guidelines of the manufacturer ...
-
bioRxiv - Neuroscience 2023Quote: Cytokine concentration levels of 5xFAD hippocampal homogenate samples were quantified using the Milliplex Map Mouse Cytokine/ Chemokine Magnetic Kit (Millipore MCYTMAG-70K-PX32), measuring a broad panel of secreted cytokines ...
-
bioRxiv - Genomics 2023Quote: ... The culture medium was then aspirated and cells were lysed by adding 600ul buffer RLT from RNeasy Mini kit with 1:100 V/V β-mercaptoethanol (Sigma M3148-250ML) to the dish ...
-
bioRxiv - Plant Biology 2023Quote: Total RNA from different tissues was extracted from 100 mg tissue using the TRIzol reagent or SpectrumTM Plant Total RNA kit (Sigma-Aldrich, United States) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the second parotid salivary gland was analyzed using the pyruvate kinase activity assay kit (cat. No. MAK072, Sigma-Aldrich, St. Louis, MO). For both activity assays ...
-
bioRxiv - Neuroscience 2023Quote: ... Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma, MX488AS100) according to manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2023Quote: Whole-thorax samples were grounded into a fine powder using a TissueLyser and used as input for the SpectrumTM Plant Total RNA Kit (Cat. No. STRN50, Sigma Aldrich, MO, USA), including DNase I treatment (Cat ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were transfected with siRNAs/plasmid as described earlier and RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit (Sigma Aldrich, # RTN70). RNA was reverse transcribe using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814) ...
-
bioRxiv - Microbiology 2024Quote: To levels of cytokines in the sera of infected animals were obtained using the MILLIPLEX® MAP Bovine Cytokine/Chemokine Magnetic Bead Panel 1 - Immunology Multiplex Assay Kit (Merck-Millipore, BCYT1-33K) and the Bovine IFN-alpha ELISA Kit (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2024Quote: The total RNA from separate achene and receptacle at six development stage with three replications per sample were extracted by the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, MO, USA) as fallowing the manufacturer protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... the cultures were washed 3 times in PBS for 5 minutes and incubated with Duolink Plus and Minus PLA probes (diluted 1:5) followed by Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, cat. no. DUO92102), according to the manufacturer’s guidelines ...
-
bioRxiv - Developmental Biology 2024Quote: ... lysates were subjected to a Millipore Luminex based Multiplex assay to measure signaling targets using the Milliplex multiple pathway cell signaling magnetic bead 9-Plex kit (Millipore Sigma, 48-681MAG) according to manufacturer’s protocol.
-
bioRxiv - Microbiology 2024Quote: Cell-supernatant was harvested at the specific time-points post-infection and the lactate content of the sample was estimated using the Lactate Assay Kit (Sigma, Catalog Number-MAK064) as per manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2020Quote: ... total RNA was isolated from the base of spruce hypocotyls (5 mm long from 36 to 38 hypocotyl cuttings) using a Spectrum™ Plant Total RNA Kit (Sigma-Aldrich; https://www.sigmaaldrich.com/). cDNA was synthesized using an iScript™ cDNA Synthesis Kit (Bio-Rad ...
-
bioRxiv - Plant Biology 2020Quote: ... An equal concentration of cDNA from the samples comparing was used for qRT-PCR reaction with KAPA SYBR Fast qPCR Kit (Sigma-Aldrich, St. Louis, USA). For expression analysis of the genes responsive to nutrient starvation ...
-
Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
-
bioRxiv - Molecular Biology 2019Quote: Protein from whole-tissue sample lysates (40 μg) were batch processed for 20S proteasome activity using commercially available fluorometric kits (catalog #: APT280; Millipore Sigma; Burlington, MA, USA) per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: The emtA gene of strain DHT3 was disrupted by an intragenic insertion of a group II intron into this gene using the TargeTron Gene Knockout System Kit (Sigma-Aldrich, St. Louis, MO). The intron insertion sites for emtA were predicted using online intron design software (TargeTron gene knockout system ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... we also determined whole-body triacylglyceride (TAG) content (in µg per fly) using a serum triglyceride determination kit (Sigma Aldrich; Tennessen et al. 2014). For each cage and treatment ...
-
bioRxiv - Immunology 2019Quote: ... PCs and B cells were detected by flow cytometry by combining surface staining with intracellular staining using the Edu-labelling kit following the manufacturer’s instructions (Sigma, EdU-Click 488/Base click).
-
bioRxiv - Bioengineering 2019Quote: ... and TIRAP (Novus NB300-990, 1:100 dilution) and analyzed via flow cytometry using a DuoLink PLA flow cytometry kit (Sigma Aldrich, St. Louis, MO). Samples were analyzed using a BD Accuri C6 Benchtop Cytometer and median fluorescence intensity was reported.
-
bioRxiv - Genetics 2019Quote: Total RNA was extracted from up to 100 mg of liquid nitrogen ground tissue using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, Taufkirchen, Germany) according to the manufacturer’s instructions for protocol B ...
-
bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no. HCVD3-67CKHCYTOMAG-60K, Millipore Corp, St. Charles, MO). According to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2019Quote: Fragmented DNA was stained by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL) – method using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit S7100 (Merck Millipore SAS, Molsheim, France) according to the manufactureŕs instructions (Fig 2 B) ...
-
bioRxiv - Physiology 2019Quote: ... Tissue samples were frozen in liquid nitrogen and total RNA was extracted with the Spectrum Plant Total RNA Kit (Sigma, St. Louis, MO, USA) according to the manufacturer’s protocols ...