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Citations for Millipore Sigma :
5651 - 5700 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated for 2 hours by the Autophagy Reagent A (rapamycin containing) from the FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (Millipore, FCCH100171), which have been diluted at 1:50 as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were lysed and the ATP content was measured by an ATP assay kit per manufacturer’s instruction (Millipore Sigma, n = 3).
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bioRxiv - Bioengineering 2020Quote: ... The alkaline phosphatase (AP) staining was performed 72 hours after seeding using the Leukocyte Alkaline Phosphatase kit (Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed a further three times in TBS-T prior to antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was undertaken using a G:BOX Chemi-XR5 (Syngene ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Cancer Biology 2020Quote: ... and processed on ABI 3500Xl Genetic Analyzer and mycoplasma testing was conducted with the Venor™ GeM Mycoplasma Detection Kit (Cat# MP0025-1kt, Sigma-Aldrich) both were done as recent as 1/9/2020 ...
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bioRxiv - Cell Biology 2021Quote: ... No contamination of mycoplasma was tested in every batch using the VenorGeM Mycoplasma Detection Kit (SIGMA-Aldrich MP-0025, St.Louis MO, USA), following the procedure indicated by the provider ...
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bioRxiv - Bioengineering 2021Quote: ... after 48 hours of incubation at 37°C were performed according to the manufacturer’s instruction (Malachite Green Phosphate Assay Kit, Sigma-Aldrich, The Netherlands). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Plant Biology 2020Quote: ... Up to 100 mg of liquid nitrogen ground tissue was applied to the SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Taufkirchen, Germany) according to the manufacturer’s instructions for protocol B ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted using a method combining CTAB lysis buffer and the Spectrum Total Plant RNA extraction kit (Sigma, Darmstadt, Germany) as described previously (83) ...
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50μg of lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from Legionella strains cultured for 18 h in AYE medium using the GenElute™ Bacterial Genomic DNA Kit (NA2100, Sigma-Aldrich) and used as template for PCR reactions ...
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bioRxiv - Microbiology 2021Quote: ... Double-stranded cDNA was synthesized and amplified using a TransPlex whole-transcriptome amplification kit (WTA-2; Sigma-Aldrich, St. Louis, MO, USA) and antibody-inactivated hot-start Taq DNA polymerase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Sulfate was determined via the barium precipitation method using the Sulfate Assay Kit following manufacturer’s instructions (Sigma Aldrich, Saint Louis, MI, USA). Sample measurements were carried out in technical triplicates.
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bioRxiv - Physiology 2021Quote: The NEFAS concentration was quantified using the Free Fatty Acid Quantitation Kit (MAK044) according to the technical bulletin (Sigma-Aldrich, United States). The results are expressed in ng/μl.
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was isolated from 27 samples (‘EMEK3’, R bulk and S bulk × 3 treatments/ sampling time × 3 replicates) using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Detection of an interaction between BRCA1 and the cavin or CAV1 proteins was assessed using the Duolink™ II Detection Kit (Sigma Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: Ect were labeled with PKH26 dye (PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Sigma Aldrich, cat. No. PKH26GL) as previously described [35] ...
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bioRxiv - Neuroscience 2020Quote: ... and COs derived from H9 hESCs and iPSCs were measured using the Human Oxidative Phosphorylation Magnetic Bead Panel Milliplex MAP Kit (Millipore–H0XPSMAG-16K), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... organelle and nuclear fractions were harvested every 12 h for 2 days using the Nuclei EZ Prep nuclei isolation kit (Sigma NUC-101) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... for amplifying the whole genome of each lysed tissue sample by the GenomePlex Single Cell Whole Genome Amplification Kit (WGA4-50RXN, Sigma-Aldrich, USA). For each sample ...
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bioRxiv - Cancer Biology 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using the ApopTag® Peroxidase in situ Apoptosis Detection Kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Golgi apparatus was isolated from mouse liver or cultured HCs by sequential sucrose gradients using a Golgi Isolation Kit (GL0010-1KT, Sigma-Aldrich, USA), based on the protocol we previously established (Tang and Wang ...
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bioRxiv - Microbiology 2021Quote: ... Following further dehydration in a graded acetone series the agarose blocs were embedded in Spurr’s resin (Spurr’s Low Viscosity embedding kit, Sigma Aldrich, St Louis, USA) using acetone as intermedium ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...
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bioRxiv - Developmental Biology 2022Quote: ... In situ hybridization was completed according to a standard protocol (Cunningham and Monk, 2018) using DIG RNA labeling kit T7/SP6 (Millipore Sigma #11175025910). RNA probes were cleaned using SigmaSpin Sequencing Reaction Clean-UP (Millipore Sigma #S5059-70EA) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Perifusate fractions were collected at the flow rate of 3ml/min and insulin was measured using radioimmunoassay kit (Millipore, Cat #: RI-13K). Insulin secretion were normalized to IEQ.
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bioRxiv - Biochemistry 2022Quote: ... The Duolink procedure was conducted according to the manufacturer’s instructions with the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Duolink®, Sigma□Aldrich), with secondary antibodies contained in the kit ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Microbiology 2022Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). All chemicals and antibiotics were obtained from Sigma unless otherwise noted.
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: The determination of telomerase enzyme activity was done in HEKn cells by using TeloTAGGG Telomerase PCR Plus Kit (Sigma Aldrich, USA, 12013789001) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The genomic DNA of kanamycin-sensitive and chloramphenicol-sensitive clones was isolated using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich), and analyzed by PCR to confirm the gene replacement using primers pairs TP_ctg3-43_f / TP_ctg3-43_r in reactions (20 μL ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 200 mg of pollen tubes using SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). RNA-seq libraries were performed using the non-stranded mRNA Kit from Illumina (San Diego ...
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bioRxiv - Microbiology 2021Quote: ... DNase I treatment was given to avoid genomic DNA contamination followed by purification of total RNA using GenElute™ Total RNA Purification Kit (Sigma-Aldrich). The purified RNA was quantified using a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Neuroscience 2021Quote: ... were measured with a human cytokine/chemokine panel utilizing Luminex 200 xMap technology and the MILLIPLEX MAP ® multiplex kit (Luminex xMAP technology; EMD Millipore, Chicago ...
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bioRxiv - Neuroscience 2020Quote: ... we spun down for 3 min at 13.000 rpm at 4° C and performed glucose assays on the supernatant using a Glucose (GO) assay kit from Sigma (GAGO-20).
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bioRxiv - Immunology 2021Quote: ... Concentrations were determined in duplicate by fluorescent-coded magnetic beads (MagPlex-C; MILLIPLEX. MAP Kit, Canine Cytokine Magnetic Bead Panel, 96-Well Plate Assay, CCYTO-90K, Millipore, Billerica, MA), based on the Luminex xMAP technology (Luminex 200 ...
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bioRxiv - Biophysics 2021Quote: The ability of SHP2 to dephosphorylate the phosphopeptides was evaluated through a malachite green phosphatase assay (PTP assay kit 1 Millipore, MA, USA). The BTAM peptide and the following monophosphorylated peptides derived from known SHP2 substrates were used for comparison ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three more times in TBS-T before antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was conducted using a G:Box Chemi-XR5 (Syngene ...
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bioRxiv - Developmental Biology 2022Quote: ... Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: The number of apoptotic cells were determined using the In Situ Cell Death Detection Kit (Sigma-Aldrich, 11684795910, St. Louis, MO, USA) in paraffin-embedded tissue sections ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Plant Biology 2019Quote: RNA was extracted from leaves and roots of sunflowers from the genotype HA412-HO using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich). Single strand copy DNA (ss-cDNA ...
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bioRxiv - Pathology 2020Quote: ... Eleven markers were simultaneously measured in the samples using a MILLIPLEX Mouse Cytokine/Chemokine 11-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2020Quote: ... Cells were allowed to adhere overnight at 26°C prior to being assessed for β-galactosidase activity associated with cellular senescence using a commercially available Senescence Cells Histochemical Staining Kit (Sigma Aldrich). The manufacturer’s protocol was followed with the following modifications ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from immature seeds of Trombay wild and TU94-2 plants with the help of Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) and treated with DNAse-I (Sigma-Aldrich ...