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Citations for Millipore Sigma :
5701 - 5750 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... in serum was analyzed by measuring 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc) using Ketone body assay kit (Sigma, No. MAK134) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 μl of CHAMP buffer containing 4 nM Alexa488-conjugated anti-Flag antibody (Alexa Fluor 488 antibody labeling kit, Thermo Fisher; monoclonal BioM2, Sigma-Aldrich) was injected to wash off unbound RNP and label DNA-bound RNP complex ...
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bioRxiv - Systems Biology 2020Quote: Plasma aliquots of blood collected from the tail vein after 4-hour fasting were used to measure the concentration of glucose and triglycerides by enzymatic colorimetric assays using commercially available kits (Sigma-Aldrich, USA) according to manufacturer’s instruction [17] ...
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bioRxiv - Neuroscience 2021Quote: ... PDMS with the standard 1:10 weight ratio of crosslinker to base (SYLGARD 184 Silicone Elastomer Kit, Dow Chemical) was diluted with hexane (Sigma-Aldrich, 34859) in a volume ratio of 1:9 ...
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50 μg lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Physiology 2020Quote: ... Secretion of glycerol and non-esterified fatty acid from cultured adipocytes into HBSS was determined with assay kits from Sigma-Aldrich (FG0100) and FUJIFILM Wako Diagnostics-NEFA Reagent (NEFA-HR(2)) ...
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bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
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bioRxiv - Microbiology 2019Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Cell Biology 2019Quote: PLA was performed according to the manufacturer’s instructions using the Duolink In Situ Orange Kit Goat/Rabbit (Sigma Aldrich, Cat. No. DUO92106). Immunofluorescence was performed as previously described (Arno et al. ...
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bioRxiv - Biochemistry 2019Quote: ... β-hydroxybutyrate (HBA) was determined by coupled spectrophotomet-ric assay with β-hydroxybutyrate dehydrogenase (obtained as kit from Sigma, St. Louis, USA). It was verified that urine does not interfere with this kit ...
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bioRxiv - Microbiology 2019Quote: ... The a-P28 antibody was conjugated with the Cy3 fluorescent dye using the Cy®3 Ab Kit GE Healthcare (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: The intracellular ATP content in the HGs was measured with an Adenosine-5’-triphosphate bioluminescent assay kit (FL-AA, Sigma Aldrich Inc.). Bioluminescent luciferase-luciferin reactions provide the basis of simple ...
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bioRxiv - Genomics 2021Quote: ChIP was performed thrice independently for each antibody using 1×106 SK-N-SH cells for each transcription factor using the EZ-Magna ChIP kit (Millipore Sigma, USA), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Ethanol 70% (vol/vol) was added and the contents were placed in columns (GenElute® mammalian total RNA miniprep kit, Sigma Aldrich). The RNA was extracted according to the manufacturer recommendations ...
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bioRxiv - Physiology 2021Quote: The G3P concentration was quantified using the Glycerol-3-Phosphate Assay Kit (MAK207) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of G3P was expressed in ng/μg protein.
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bioRxiv - Physiology 2020Quote: ... TUNEL staining was performed using a protocol modified from the ApopTag peroxidase in situ apoptosis detection kit (Merck-Millipore, Cat no. S7100). Paraffin sections of adipose tissues were prepared as above ...
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bioRxiv - Physiology 2021Quote: ... A 30-μL portion of supernatant was used to measure circulating glucose levels with glucose oxidase assay kit (Sigma-Aldrich, GAGO-20) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Aforementioned single clone cultures were STR validated and screened for any mycoplasma contamination using PCR based Myco detection kit (Sigma, MP0035-1KT) before use ...
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bioRxiv - Cancer Biology 2021Quote: ATP levels were measured in control and constricted breast cancer cells (3 x 105 cells) using the ATP fluorometric assay kit (Sigma-Aldrich, MAK190), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Reduced and oxidized fractions of nicotinamide adenine dinucleotide phosphates (NADPH/NADP) were measured using the NADP/NADPH Quantitation kit (Sigma-Aldrich, USA) adhering to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-17A and TNFα were quantified in culture supernatants using the mouse-specific Milliplex® multi-analyte panel kit MT17MAG-47K (Millipore; Sigma) and the MagPix® instrument platform with related xPONENT® software (Luminex Corporation) ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Fluorescent membrane labelling of the VE was achieved using the CellVue Claret Far Red Fluorescent Cell Linker Kit (Sigma-Aldrich, MINICLARET-1KT). Briefly ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Cell Biology 2020Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Genomics 2021Quote: ... These pX330-mC plasmid DNAs and donor DNA plasmid were isolated using FastGene Plasmid Mini kit (Nippon Genetics, Tokyo, Japan) and filtered using MILLEX-GV® 0.22 μm filter unit (Merck Millipore, Darmstadt, Germany) for microinjection ...
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bioRxiv - Immunology 2021Quote: ... Bulk endotoxin-free preparations of VIU-1005 and the empty control plasmid (control) were prepared for animal studies using GenElute™ HP Select Plasmid Gigaprep Kit (Sigma, Germany).
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA isolated from a 5-mL marine broth overnight culture was extracted with the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) and used as template ...
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bioRxiv - Immunology 2021Quote: The levels of cytokine and chemokines in BAL samples from NV1-treated and control SARS-CoV-2-infected mice were analyzed by Luminex multiplex assays using 36-plex MILLIPLEX® MAP Mouse Cytokine / Chemokine Magnetic Bead kit (EMD Millipore). BAL samples were quickly vortexed for 10s ...
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bioRxiv - Genomics 2021Quote: ... For the VPA treatment hiPSCs were differentiated to endoderm using a 2-day DE Induction Kit following the manufacturer’s instructions and from day 0 of differentiation 400uM VPA (valproic acid sodium salt 98%, Sigma Cat. # P4543-10G) in DMSO was added to cell cultures.
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... DNA amplicons and related donor template levels were evaluated by semiquantitative PCR and qPCR (using KAPA SYBR FAST qPCR Kits, Sigma-Aldrich, USA), respectively ...
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bioRxiv - Microbiology 2020Quote: ... Templates used were either bacterial DNA extracted with the GenElute™ Bacterial Genomic DNA Kit or phage DNA extracted from phage lysates that had been precipitated as previously described and then extracted using the GenElute™ Bacterial Genomic DNA Kit with an extended proteinase K incubation of 1 hour (Sigma-Aldrich). 50 ng of template was used for each PCR ...
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bioRxiv - Microbiology 2020Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). Chlamydia trachomatis strain L2/434 Bu was obtained from the laboratory of Dr ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
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bioRxiv - Biochemistry 2022Quote: ... we first extracted the gDNA from each cellular fraction using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2022Quote: We analyzed chemotherapy responses of human leukemia cell lines expressing wild-type NT5C2 or NT5C2 mutant constructs following 72-hour incubation with increasing concentrations of 6-mercaptopurine by measuring metabolic reduction of the tetrazolium salt MTT using Cell Proliferation Kit I (Sigma-Aldrich, #11465007001) following manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The column was calibrated using a gel filtration markers kit for protein molecular weights 29,000-700,000 Da (Sigma-Aldrich, product number MWGF1000).
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bioRxiv - Plant Biology 2022Quote: ... 48 and 81) from ten time points during autumn (225-270 DOY) 2018 using Spectrum™ Plant Total RNA isolation kit (Sigma-Aldrich) according to manufacturer’s protocol (n=3 individual trees per genotype and time point) ...
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bioRxiv - Microbiology 2022Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of paraffin-embedded sagittal sections was performed using Apoptag Peroxidase In Situ Apoptosis Detection Kit (EMD Millipore, S7100), per the manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... and 100 mg of ground sample was processed using the Spectrum™ plant total RNA kit (Sigma-Aldrich, St. Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... glycerol and triglyceride levels in each sample were measured using a Serum Triglyceride Determination Kit (Sigma-Aldrich, components F6428, T2449, and G7793) and normalized to protein levels (BCA Protein assay ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with propidium iodide and Hoechst 33342 from the Live-Dead Cell Viability Assay Kit (Millipore Sigma, Catalog CBA415) according to manufacturers’ instructions and immediately imaged at 10x magnification with a 0.17 numerical aperture CFI60 objective on a Keyence BZ-X710 microscope ...
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bioRxiv - Microbiology 2022Quote: Water samples collected from each site were vacuum filtered using the Millipore All-Glass Filter Holder kit (EMD Millipore Corporation, Billerica, MA). Collected water from each replicate was filtered through Whatman filter membrane to remove coarse to medium debris ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Determination of SL drug cytotoxicity in 2D samples was performed using the cell counting kit-8 (CCK8) viability assay (Sigma Aldrich, #96992). CCK8 uses water-soluble tetrazolium to quantify the number of live cells by producing formazan dye generated by dehydrogenases in cells ...
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bioRxiv - Plant Biology 2024Quote: ... the total proteins in the samples were measured through the BiCinchoninic acid Assay using a kit (Sigma-Aldrich, Saint Louis, MO, USA). The assay was conducted according to the method provided by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... pyruvate and isocitrate concentration was determined by colorimetric assay kits that were used according to the respective manufacturer’s protocol (Sigma; MAK086, MAK071, MAK061). 1 g of soil was mixed with 1 ml of VE H2O ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... The genomic DNAs were extracted from the clones with the GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO). The genome sequences were examined with PCR using the following primers ...
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bioRxiv - Immunology 2024Quote: ... Mice were injected with approximately 107 total IU of lentivirus measured by qPCR Lentivirus Titer kit (abm, #LV900) and delivered by two 10 µL injections using a 25 µL Hamilton syringe (Sigma-Aldrich, #20787) on a stereotaxic alignment system (Kopf ...