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Citations for Addgene :
3701 - 3750 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: GFP nanobody beads were prepared from vector pGEX6P1-GFP-Nanobody (Addgene #61838) as per the protocol.57 The beads were stored in 20% ethanol made in PBS as 1:1 slurry ...
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bioRxiv - Cell Biology 2024Quote: ... viral packaging plasmid (pspax, Addgene #12260), and viral envelope plasmid (pMD2.G ...
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bioRxiv - Cell Biology 2024Quote: ... were transfected using Lipofectamine 3000 as per manufacturer’s instructions with plasmids having Cas9 and gRNA (Addgene #97081) and a plasmid having repair template (Addgene #97088 ...
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bioRxiv - Cell Biology 2024Quote: ... gRNAs were cloned into lenti sgRNA(MS2)_zeo (Addgene #61427) backbone using BsmBI restriction enzyme site ...
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bioRxiv - Genetics 2024Quote: ... these four amplicons were placed into pCFJ151 (Addgene #19330) digested with AflII (New England BioLabs ...
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bioRxiv - Genetics 2024Quote: promoter was amplified from pJA252 (Addgene #21512) using the primers P19 and P20 ...
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bioRxiv - Genetics 2024Quote: ... The pCS2-nCas9n expression vector was used to produce Cas9 mRNA (Addgene, #47929)110 ...
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bioRxiv - Genetics 2024Quote: ... This sgRNA was in vitro transcribed from a SP6 transcription template amplified from pDD162 (Addgene #47549) using primers P48 and P40 ...
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bioRxiv - Genetics 2024Quote: ... were mixed with 500ng pCAG-loxp-stop-loxp-ZsGreen (Addgene, 51269) plasmid ...
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bioRxiv - Genetics 2024Quote: Guide RNA sequences were cloned into pCFD4-U6:1_U6:3tandemgRNAs was a gift from Simon Bullock (Addgene plasmid # 49411; http://n2t.net/addgene:49411 ; RRID:Addgene_49411) (Port et al ...
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bioRxiv - Genetics 2024Quote: ... Self-purified Protein AG-MNase (pDNA was from Addgene; 123461) was used ...
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bioRxiv - Genetics 2024Quote: ... The promoter region from the pCAGGS-mCherry vector (Addgene #41583) was combined with the human ACE2 CDS (Addgene #1786 ...
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bioRxiv - Genetics 2024Quote: ... breast cell lines were transduced with Lenti-Cas9-2A-Blast (Addgene #73310) or Lenti-dCas9-KRAB-Blast (Addgene #89567) ...
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bioRxiv - Genetics 2024Quote: ... lentiviral pooled library was obtained from Addgene (Addgene, # 1000000048) and was prepared as previously described 41 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or the human IFN-beta promoter (IFN-Beta-pGL3, Addgene #102597), renilla luciferase fused to herpes simplex virus thymidine kinase promoter (pTK-Renilla ...
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bioRxiv - Evolutionary Biology 2024Quote: ... human RIPK3 (Addgene #78804), mouse RIPK1 (Addgene #115341) ...
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bioRxiv - Neuroscience 2024Quote: ... and the duplexes were cloned into PX330 (Addgene, #158973) by BbsI site ...
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bioRxiv - Neuroscience 2024Quote: ... after which they were injected with adeno-associated virus (AAV-PHP.eB-syn-jGCaMP7s, Addgene) using a 30-gauge needle ...
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bioRxiv - Microbiology 2024Quote: ... programmed frameshift sites of each nidovirus were placed between the coding sequence of EGFP and mCherry (parental Addgene plasmid # 8780362) by Gibson assembly such that EGFP would be produced in 0-frame and mCherry in –1-frame with the −1PRF sequence50,51 ...
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bioRxiv - Microbiology 2024Quote: Guide RNA Plasmids: Pss013 CrispR guide vector was purchased from Addgene and digested with BsaI ...
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bioRxiv - Genetics 2024Quote: ... early passage primary MEFs from all genotypes were infected in parallel with the SV40 large T antigen (Addgene:13970) (Zhao et al. ...
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bioRxiv - Genetics 2024Quote: ... RRID:Addgene174038).23 BPK1520 was a gift from Keith Joung (Addgene plasmid #65777 ...
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bioRxiv - Genetics 2024Quote: pCMV-PEmax-P2A-GFP was a gift from David Liu (Addgene plasmid #180020 ...
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bioRxiv - Genetics 2024Quote: ... RRID:Addgene174824).22 The pU6-tevopreq1-GG-acceptor was a gift from David Liu (Addgene plasmid #174038 ...
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bioRxiv - Genetics 2024Quote: ... and blue fluorescent protein (tagBFP) with a KRAB domain at the C-terminus (Addgene 167981; Figure 1A)14 ...
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bioRxiv - Genetics 2024Quote: We use two constructs for epigenome editing: 1) dCAS9-DNMT3A/3L co-expressed with enhanced green fluorescent protein (eGFP) for selection 12 (Addgene, Catalogue number 128424), and 2 ...
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bioRxiv - Genetics 2024Quote: ... the top two candidate sgRNA sequences for each gene were cloned into lentiCRISPRv2 (Addgene # 52961) (Sanjana ...
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bioRxiv - Genetics 2024Quote: ... RRID:Addgene180020).22 pEF1a-hMLH1dn was a gift from David Liu (Addgene plasmid #174824 ...
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bioRxiv - Genetics 2024Quote: ... Guide RNAs were ordered as dsDNA from Integrated DNA Technologies (Supplemental Table S1) and cloned into the expression vector (Addgene 44248) by double digest with BstXI and NotI (Thermo Fischer Science) ...
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bioRxiv - Genetics 2024Quote: ... or Lenti-dCas9-KRAB-Blast (Addgene #89567). MCF7 cells stably expressing Cas9 or KRAB-dCas9 were established by transduction of the virus at MOI<0.7 29 ...
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bioRxiv - Genetics 2024Quote: ... Pooled oligonucleotides were cloned into the BsmBI sites of pLeniGuide-Puro (Addgene #52963). We included as positive controls core cell essential genes and genes known to confer resistance to T cell-mediated killing ...
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bioRxiv - Immunology 2024Quote: ... Lentivirus was produced using standard virus production methods by co-transfecting target and packaging plasmids (psPAX2 – Addgene12260 and pMD2.G-Addgene 12259) into HEK293T cells ...
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bioRxiv - Immunology 2024Quote: Plasmids pMD2.G and psPAX2 were gifts from Didier Trono (Addgene plasmid # 12259; # 12260). Full length FcγRIIIa variant sequences were cloned under the CMV promoter in pCDH-CMV-MCS- EF1α-copGFP (System Biosciences) ...
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bioRxiv - Genomics 2024Quote: ... and a transfer vector (Fuw-dCas9-DNMT3A-P2A-tagBFP -Addgene #84569-; Fuw-dCas9-TET1-P2A-tagBFP -Addgene #108245- and pLV GG hUBC-dsRED -Addgene #85034- ...
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bioRxiv - Genetics 2024Quote: The human GeCKO-v2 (Genome-Scale CRISPR Knock-Out) lentiviral pooled library was obtained from Addgene (Addgene, # 1000000048) and was prepared as previously described 41 ...
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bioRxiv - Genetics 2024Quote: ... 5’- and 3’-homologous arms were added to both ends. Two gRNA sequences were cloned into plasmid pCFD4 ((Port et al. 2014) (Addgene). The donor and gRNA plasmids were injected into a Cas9 line (y[1] M{vas-Cas9.S}ZH-2A w[1118] ...
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bioRxiv - Genetics 2024Quote: The CAG-ACE2 genetic construct was prepared on the basis of the pT2/BH backbone (BglII, EcoRI sites) (Addgene #26556). The promoter region from the pCAGGS-mCherry vector (Addgene #41583 ...
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bioRxiv - Genomics 2024Quote: ... GRCh38.p13 (human) and eGFP (sequence obtained from FUGW Addgene #14883) and tdTomato (sequence obtained from pCSCMV ...
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bioRxiv - Genetics 2024Quote: Guide RNA sequences were cloned into pCFD4-U6:1_U6:3tandemgRNAs was a gift from Simon Bullock (Addgene plasmid # 49411 ...
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bioRxiv - Genomics 2024Quote: ... and p306-BrdU-Inc (Addgene plasmid # 71792; http://n2t.net/addgene:71792; RRID:Addgene_71792) vectors ...
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bioRxiv - Genomics 2024Quote: ... and a transfer vector (Fuw-dCas9-DNMT3A-P2A-tagBFP -Addgene #84569-; Fuw-dCas9-TET1-P2A-tagBFP -Addgene #108245- and pLV GG hUBC-dsRED -Addgene #85034-) using calcium phosphate ...
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bioRxiv - Genomics 2024Quote: ... and phU6-gRNA (Addgene #53188)] using a BbsI restriction enzyme site ...
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bioRxiv - Genomics 2024Quote: ... and a transfer vector (Fuw-dCas9-DNMT3A-P2A-tagBFP -Addgene #84569- ...
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bioRxiv - Genomics 2024Quote: ... each protospacer was first annealed and cloned into the desired expression vector [phH1-gRNA (Addgene # 53186); ph7SK-gRNA (Addgene #53189) ...
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bioRxiv - Genomics 2024Quote: ... oligonucleotide pairs encoding IL1RN shRNAs were annealed and cloned into pSICOR-PGK-puro (Addgene #12084). pSicoOligomaker 1.5 (https://venturalaboratory.com ...
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bioRxiv - Genomics 2024Quote: ... Multiplex sgRNA-dsRED (pVL GG hUBC-dsRED plasmid -Addgene # 84034-) construct was cloned using a two-step protocol as described by Engler and coworkers73,74 ...
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bioRxiv - Genetics 2024Quote: ... and a GFP amplicon with a minimal TATA promoter (amplified from pMPRAv3:minP-GFP, Addgene #109035) was inserted using Gibson assembly (125uL 2xNEB HiFi Assembly MM ...
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bioRxiv - Genomics 2024Quote: ... pmU6-gRNA (Addgene # 53187), and phU6-gRNA (Addgene #53188)] using a BbsI restriction enzyme site ...
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bioRxiv - Genetics 2024Quote: ... and helper plasmids (psPAX2, pMD2.G, Addgene) were transfected into HEK293T cells in 6-well plate at 70% confluence using lipofectamine 3000 reagents ...
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bioRxiv - Genetics 2024Quote: ... Human TLK2 pDONR223 was a gift from William Hahn & David Root (Addgene: Plasmid #23629). Human LC8 expression vectors and its mutants were described previously (42) ...