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Citations for Addgene :
3701 - 3750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: UMUC-3 TM4SF1-KO cells were generated by transient transfection (Lipofectamine 3000) of UMUC-3 cells with PX458 (Addgene, #48138). Each plasmid contained one of three different sgRNA targeting sequences ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng purified oligo were mixed with 5 μg lentiCRISPR v2 (Addgene #52961) backbone ...
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bioRxiv - Cancer Biology 2024Quote: ... 9μg psPAX2 (Addgene, 12260) and 3μg pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: U2OS cells infected with lentivirus expressing shRNA targeting “scramble” control sequence (AddGene Plasmid #1864 ...
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bioRxiv - Cancer Biology 2024Quote: ... Atg7 knockout cell lines were generated by transducing cells with lentiviral CRISPR/Cas9 (TLCV2 Addgene plasmid #87360). Lentiviruses were generated using 293T cells via transfection with PEI ...
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bioRxiv - Cancer Biology 2024Quote: ... The pLKO.1 shRNA plasmid targeting human TSC2 was a gift from Do-Hyung Kim (Cat#15478, Addgene). Lentiviral pLKO shRNA plasmids were transiently co-transfected individually along with plasmids encoding ΔVPR and VSV-G into HEK293T cells using TurboFect™ (Cat#R0531 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected using standard polyethylenimine (PEI) protocols in suspension at time of seeding with 30 ng reporter HRELuc (Addgene #26731 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1.5 μg of psPAX2 (AddGene, #12260) mixed in 0.8 ml Opti-MEM medium (Thermo ...
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bioRxiv - Cancer Biology 2024Quote: ... directed to GFP as non-targeting control (ΔGFP) or to human GDF15 (ΔGDF15) were cloned into the BsmBI (Thermo, #ER0451) site of lentiCRISPRv2-puro vector (AddGene, #52961) using the following pairs of annealed oligonucleotides obtained from IDT for GFP (forward 5’-CACCGGTGAACCGCATCGAGCTGA-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... and EGFP-Rab7A (RRID: Addgene 28047), EGFP-N1 (RRID ...
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bioRxiv - Neuroscience 2024Quote: Mammalian-codon optimized HcKCR1 (GenBank MZ826861) and WiChR (Addgene #195190/GenBank OP710241) were expressed in rodent neuroblastoma cells similar as previous described (Vierock et al ...
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bioRxiv - Neuroscience 2024Quote: ... and pCXLE-EGFP obtained from Addgene. Human adult fibroblasts collected from unaffected donors (n = 3 ...
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bioRxiv - Neuroscience 2024Quote: ... a gift from Johannes Zuber (Addgene plasmid # 111162) 95 and then into 3’-UTR of GFP in pAAV-Ef1a-DIO GFP plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... we injected 0.3 μL AAVs encoding ChR2(H134R)-eYFP (titer = 1 x 1012 GC/ml, Addgene# 26973, RRID: Addgene_127090) into the layer 5 of medial prefrontal cortex (mPFC ...
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bioRxiv - Neuroscience 2024Quote: ... Addgene catalog: #20298-AAV1) or eYFP as control (AAV5-EF1a-DIO-eYFP; Addgene catalog: #27056-AAV5) were injected ...
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bioRxiv - Neuroscience 2024Quote: ... An intersectional approach was used for selective activation of mPFC to BLA projection using chemogenetics: 1) retrograde AAV encoding FlpO (0.3 μL, titer ≥ 7 x 1012 GC/ml, Addgene# 55637; RRID:Addgene_55637) were bilaterally injected into BLA (A-P ...
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bioRxiv - Neuroscience 2024Quote: ... PV-ChR2 mice were injected with 1µl of AAV-CaMK2-GCaMP6f (Addgene, #100834-AAV9, dilution 1/15) at 0.75µl.min−1 in the left primary auditory cortex (centered at 1.75 mm anterior to the inter-section of the lambdoid and interparietal-occipital sutures ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV.Dlx.DIO.GFP.N2cG was constructed by Gibson assembly the PCR fragment GFP-P2A-N2cG from pAAV-VTKS2-TVA-eGFP-N2cG (Addgene #175439) into the backbone pAAV-VTKD2 (Addgene #170847)65 digested by EcoRI and HindIII.
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bioRxiv - Neuroscience 2024Quote: ... The rabies virus construct was a gift from Thomas Jessell (Addgene #7347180) and EnvA-pseudotyped CVS-N2c(ΔG)-FlpO-mCherry was generously shared by K ...
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bioRxiv - Neuroscience 2024Quote: ... then inserting the jGCaMP8m66 fragment from pGP-AAV-syn-jGCaMP8m-WPRE (Addgene #162375) digested by EcoRI and HindIII ...
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bioRxiv - Neuroscience 2024Quote: ... into the backbone pAAV-EF1a-flex-TVA (Addgene #69618), digested by EcoRI and HindIII ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9 helper (Addgene plasmid #112865, 9 μg/dish), and the AAV expression vector (12 μg/dish) ...
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bioRxiv - Neuroscience 2024Quote: ... into the backbone pAAV-VTKD2 (Addgene #170847)65 digested by EcoRI and HindIII.
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bioRxiv - Neuroscience 2024Quote: ... AAV5/Syn-Flex-GCaMP6s-WPRE-SV40 (Addgene, Cat. #100845) were unilaterally injected into the mPFC (+1.8 anteroposterior ...
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bioRxiv - Neuroscience 2024Quote: ... Horizon) according to manufacturer’s instructions and with 1.125 µg/mL tyrosinated tubulin sensor TagRFP-T AlaY1 (158751; Addgene) (Kesarwani et al. ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-dLight1.1 (Addgene #111066), and pHelper (CELL BIOLABS ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-dLight1.1 was a gift from Lin Tian (Addgene plasmid #111066 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV2/9n (Addgene #112865), pAAV-hSyn-dLight1.1 (Addgene #111066) ...
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bioRxiv - Neuroscience 2024Quote: ... Avpr1b-Cre+/- mice were injected with either a AAV8-EF1a-DIO-hChR2(H134R)-mCherry (Addgene, #20297 ...
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bioRxiv - Neuroscience 2024Quote: ... cohort 1) or AAV9.EF1a.dflox.hChR2(H134R).mCherry.WPRE.HGH (1×10^12 pp/mL, Addgene, cohort 2).
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bioRxiv - Neuroscience 2024Quote: ... or a AAV5-EF1a-DIO-hChR2(H134R)-eYFP (Addgene, #20298 ...
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bioRxiv - Neuroscience 2024Quote: Flag-Human CXCR7(ACKR3)-Tango (Plasmid #66265, Addgene) immune-purification was performed as described in 87 ...
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bioRxiv - Neuroscience 2024Quote: ... which was a gift from Jonathan Ting (Addgene viral prep #51085-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-GfaABC1D-tagBFP-miR30 plasmid was generated by combining the tagBFP sequence with the miR30 sequence (Horizon, pGIPZ plasmid) using overlapping PCR and subcloned into pAAV-GfaABC1D-eGFP plasmid (Addgene #176861). The pAAV-GfaABC1D-tagBFP-miR30-scramble and pAAV-GfaABC1D-tagBFP-miR30-shAckr3 constructs were subcloned into the pAAV-GfaABC1D-tagBFP-miR30 vector with the following target sequences.
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bioRxiv - Neuroscience 2024Quote: ... a helper plasmid (Addgene #112867), and a pAAV9 capsid plasmid (Addgene #198016 ...
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bioRxiv - Neuroscience 2024Quote: ... the adeno-associated virus (AAVs) pGP-AAV-syn-jGCaMP7s-WPRE (AAV1; Addgene) was injected (1:10 in saline ...
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bioRxiv - Neuroscience 2024Quote: ... and a pAAV9 capsid plasmid (Addgene #198016) with polyethylenimine (PEI ...
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bioRxiv - Neuroscience 2024Quote: ... INTRSCT construct (pHP.eb)hSyn.Cre-on/Flp-on.YFP (Addgene 55650)62.
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bioRxiv - Neuroscience 2024Quote: ... USA) and CXCR7-Tango plasmid (Flag-tagged ACKR3; Plasmid #66265, Addgene) were used for mammalian expression of the eGFP and Flag-ACKR3 protein ...
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bioRxiv - Neuroscience 2024Quote: ... To trace dendritic elements from SST or PV positive neurons in the STED experiment we inject AAV1.Flex.tdTomato (Addgene-28360) in S1-BF.
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bioRxiv - Neuroscience 2024Quote: ... and VSV-G (Addgene, #12259) and transduced into sub-confluent HEK293TN cells using PolyJet (SignaGen Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... the vector was packaged into lentiviral particles using psPAX2 (Addgene, #12260) and VSV-G (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... or the double floxed control vector carrying a cDNA for mCherry alone (pAAV5-hSyn-DIO-mCherry, abbreviated here as Ctrl or control vector, #50459-AAV5, Addgene, Watertown, MA), using 0.3 μL of virus per site at a titer of > 1–2 x 10¹3 vg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9 Syn-Flex-GCaMP6s-WPRE-SV40 (2.5x1013 AddGene-100845) was used respectively ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9-hsyn-DIO-HM4D(Gi)-mCherry and AAV9-hsyn-DIO-HM3D(Gq)-mCherry were obtained from Addgene (viral prep #50459 ...
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bioRxiv - Neuroscience 2024Quote: We obtained the following AAV vectors from Addgene (titer is shown as genome particles [gp] per ml) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.85 μl of virus coding for either muscarinic-receptor-based DREADD AAV8-hSyn-hM4Di(Gi)-mCherry or control virus AAV8-hSyn-EYFP (AddGene, Cambridge, MA) was injected bilaterally into either the mPFC (AP ...
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bioRxiv - Neuroscience 2024Quote: ... we employed Vgat-NAc-ChR2 mice in which we injected AAV5-EF1a-DIO-hChR2(H134R)-eYFP (UPenn Vector Core or Addgene, titers ...
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bioRxiv - Neuroscience 2024Quote: ... we stereotaxically injected 400nl of AAV1-syn-Flex-GCaMP6f-SV40 (100833-AAV1, Addgene) into the dorsolateral striatum (from bregma ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3μl pGP-AAVrg-hSyn-hChR2(H134R)-EYFP virus (7× 1012GC/ml; Addgene) or a fluorophore only control ...