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Citations for Addgene :
3601 - 3650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: An expression vector for Akt-PH-Clover was constructed as follows: The DNA encoding Clover was PCR-amplified using pcDNA3 Clover (Lam et al., 2012)(Addgene #40259 ...
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bioRxiv - Cell Biology 2024Quote: ... and pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE (Addgene plasmid #60906; RRID: Addgene_60906) were generously provided by Ron Vale (Tanenbaum et al. ...
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bioRxiv - Cell Biology 2024Quote: The PA-mCherry1-N1 vector was a gift from Michael Davidson (Addgene, 54507 ...
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bioRxiv - Cell Biology 2024Quote: ... which was kindly provided by Kevin Dean and Reto Fiolka (Addgene plasmid #110718; RRID: Addgene_110718) (Dean et al. ...
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bioRxiv - Cell Biology 2024Quote: pLenti-CMV-MCS-GFP-SV-puro (Addgene plasmid #73582) was a gift from Katarzyna Mleczko-Sanecka ...
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bioRxiv - Cell Biology 2024Quote: ... GFP in the pcDNA3 Akt-PH-GFP plasmid (Kwon et al., 2007)(Addgene #18836 ...
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bioRxiv - Cancer Biology 2024Quote: ... packaging plasmid psPAX2 (Addgene plasmid #12260), and envelope plasmid pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cancer Biology 2024Quote: ... the LentiCRISPRv2 (Addgene plasmid #52961) vector was digested with BsmBI and ligated with BsmBI-compatible pre-annealed oligonucleotides (Sanjana et al. ...
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bioRxiv - Cell Biology 2024Quote: cDNA for CENP-T full-length sequence was cloned into pAID1.2-CMV-NmScarlet-mAID (Addgene; #140618). To express the OsTIR1-T2A-BSR and mScarlet-mAID-CENP-T under control of the CMV promoter from the PGK locus ...
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bioRxiv - Cancer Biology 2024Quote: ... then transfected with Lipofectamine 3000 according to the manufacturer’s protocol with 2.5 µg of a plasmid containing Sleeping Beauty transposase (SB100X, AddGene #34879) and 2.5 µg of a plasmid containing either GFP or mCherry on a nuclear localization signal (NLS ...
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bioRxiv - Cancer Biology 2024Quote: ... and transfected with 2.5 µg linearized RUNX1 HDR template and 4 µg each of pCW-Cas9 plasmid (Addgene, #50661) as well as two sgRNA plasmids using program CD-118 of the 4D Nucleofector system (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: pGEX-KG-D4H*-mCherry plasmid (Addgene #134604) was used to purify D4H*-mCherry as previously described (32) ...
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bioRxiv - Cell Biology 2024Quote: ... psPax2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Cell Biology 2024Quote: ... and pMD2.G (Addgene #12259) using 1 mg/ml polyethylenimine ...
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bioRxiv - Cancer Biology 2024Quote: ... were cloned into the lentiCRISPRv2 plasmid (Addgene, Watertown, MA), referred as “v2” here ...
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bioRxiv - Cancer Biology 2024Quote: ... using the pVSVg and psPAX2 viral packaging plasmids (Addgene) and Effectene transfection agent (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... hSTARR-ORI vector (Addgene #99296) was linearized by dual restriction enzyme digestion with AgeI-HF (NEB cat ...
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bioRxiv - Biophysics 2024Quote: ... studies with an anti-YFP-nanobody (Addgene plasmid #61838) (Hartano et al ...
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bioRxiv - Biophysics 2024Quote: ... The HaloTag-beta2-adrenergic receptor was a gift from Catherine Berlot (Addgene plasmid # 66994).
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bioRxiv - Cancer Biology 2024Quote: ... The empty vector construct used for 293T experiments was pCMV-Neo-Bam (Addgene 16440), which has the same major functional elements as pcDNA3.1 with the exception of an HSV TK promoter for the Neo resistance gene in place of an SV40 promoter ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentivirus was packaged in 293T cells cotransfected with pHEF-VSVG (Addgene#22501) and pPax2 (Addgene#12259 ...
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bioRxiv - Cancer Biology 2024Quote: ... The corresponding empty vector pMY-IRES-EGFP was purchased from Addgene (163361). Plasmid sequences were verified via Whole Plasmid Sequencing by Plasmidsaurus/Primordium Labs using Oxford Nanopore Technology with custom analysis and annotation ...
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bioRxiv - Cancer Biology 2024Quote: ... and pPax2 (Addgene#12259) in PEI transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 packaging plasmids (Addgene, Cat.12260), using extreme Xp transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: Lentivirus particles were generated in HEK293T cells following transfection with the CAR plasmid along with pMD2.G envelope (Addgene, Cat.12259) and psPAX2 packaging plasmids (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... a gift from Cindy Miranti (Addgene plasmid # 85966; https://www.addgene.org/85966; RRID: Addgene_85966). shRNA targeting sequences (with RNAi consortium ID ...
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bioRxiv - Cancer Biology 2024Quote: ... a gift from Cindy Miranti (Addgene plasmid # 85966 ...
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bioRxiv - Cancer Biology 2024Quote: ... a gift from Bob Weinberg (Addgene plasmid # 8453; http://https://www.addgene.org/8453; RRID: Addgene_8453). For doxycycline-induced knockdown of UTX/KDM6A ...
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bioRxiv - Cancer Biology 2024Quote: ... pLenti-trGluc (Addgene # 158959), and pX330-gRNA (Addgene # 158973 ...
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bioRxiv - Cancer Biology 2024Quote: ... Clones expressing EGFP (FUGW virus; Addgene #14883) and TdTomato (FUtdTW virus ...
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bioRxiv - Cell Biology 2024Quote: ... The third generation HIV-1 derived lentiviral vector LeGO-iG2-Puro+-Luc2 expresses the Luc2 variant of firefly luciferase (cloned from AddGene Plasmid 24337) under the control of an SFFV-promoter ...
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bioRxiv - Cancer Biology 2024Quote: ... and PEI transfected into HEK293T cells along with pUMVC retroviral packaging construct (Addgene #8449) and CMV5-VSVG envelope construct (Addgene #8454) ...
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bioRxiv - Cancer Biology 2024Quote: Guide RNA (gRNA) sequences targeting neutral sphingomyelinases 1 & 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene (52961) 45 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 3μg pMD2.G (Addgene, 12259) using Xfect Transfection Reagent (Takara ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G envelope construct (Addgene #12259). Lentivirus containing media was harvested at 72-h ...
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bioRxiv - Cancer Biology 2024Quote: sgRNA sequences targeting KSR1 or non-targeting control (NTC) were inserted into TLCV2-RB1 (pLentiCRISPRv2GFP modified with puromycin selection marker) (Addgene #87836). The constructs were PEI transfected into HEK293T cells along with psPAX2 lentiviral packaging construct (Addgene #12259 ...
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bioRxiv - Cancer Biology 2024Quote: sgRNA sequences targeting KSR1 or non-targeting control (NTC) were inserted into pLentiCRISPRv2GFP (Addgene #82416). The constructs were polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G envelope construct (Addgene #12259). Lentivirus-containing media was harvested at 72-hour post-transfection and used to infect the H82 cells with 8 µg/mL Polybrene (Sigma #TR1003G) ...
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bioRxiv - Cancer Biology 2024Quote: ... transfected into HEK293T cells along with psPAX2 lentiviral packaging construct (Addgene #12259) and pMD2.G envelope construct (Addgene #12259) ...
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bioRxiv - Cancer Biology 2024Quote: ... and CMV5-VSVG envelope construct (Addgene #8454). Retrovirus-containing media was harvested at 96 hour and added to KSR1 KO cells with 8 mg/ml polybrene ...
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bioRxiv - Cell Biology 2024Quote: ... mScarlet-i-PLEKHA5WW was made by inserting the cDNA sequence encoding the tandem WW domain (residue 1-105) into the CMV-mScarlet-i-C1 vector (Addgene 85044) using BamHI and SalI ...
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bioRxiv - Cell Biology 2024Quote: ... a gift from the De Camilli lab at Yale University) into the miRFP670-N1 vector (Addgene 79987) using SalI and ApaI.
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bioRxiv - Cell Biology 2024Quote: ... Plasmids used were coding for HA-tagged KDM4A/JMJD2A (Addgene #24180) or for an irrelevant HA-tagged construct HA-Tsg5’ as negative control (24).
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bioRxiv - Cell Biology 2024Quote: ... 5HT6-G-GECO (Addgene plasmid #47499), Epac-SH187 and Epac-SH188 (gift from Kees Jalink ...
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bioRxiv - Cell Biology 2024Quote: ... followed by digestion of amplified PCR product with AgeI and NotI and ligation C-terminal to Smo in Smo-mCherry (Addgene plasmid 55134 ...
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bioRxiv - Cell Biology 2024Quote: H2B-tagBFP was cloned by inserting the H2B cDNA (amplified from pLenti6-H2B-mCherry, Addgene 89766) into the tagBFP-N1 vector (Evrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Then the entire Lyn10-TurboID-V5 cassette was inserted into the pSBtet-Pur plasmid (Addgene 60507) linearized by NcoI and BspDI to replace the firefly luciferase downstream of the Tet-responsive promoter ...
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bioRxiv - Cell Biology 2024Quote: ... pCDNA3-Lyn10-TurboID-V5 was cloned by replacing the C1(1-29) sequence in C1(1-29)-TurboID-V5-pCDNA3 (Addgene, 107173) with a Lyn10 tag (amino acid sequence GCIKSKRKDG) ...
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bioRxiv - Cell Biology 2024Quote: Plasmids expressing GST-RILP (obtained from Christopher Burd, Yale University) in pGEX-KG vector (Addgene, 77103) or pGEX KG expressing GST alone was transformed into E.coli strain BL21 (DE3) ...
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bioRxiv - Cell Biology 2024Quote: ... AAV-PHP.S-tdTomato (Addgene, 59462- PHP.S, titer ≥ 1×10¹³ vg/mL) was utilized ...