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Citations for Addgene :
3551 - 3600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... eGTACR1 was then amplified from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGTACR1-ST (a gift from Dr. Hillel Adesnik; Addgene plasmid # 109048) with a BmtI restriction site on the 5’ end and a AflII restriction site on the 3’end ...
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bioRxiv - Microbiology 2024Quote: ... and psPAX2 (Addgene, #12260) packaging plasmids using JetOptimus DNA transfection reagent (Polyplus ...
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bioRxiv - Developmental Biology 2024Quote: ... Tg(dand5:EGFP) embryos were injected with bungarotoxin messenger-RNA (Addgene catalogue #69542) at the one-cell stage to immobilize them.
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bioRxiv - Plant Biology 2024Quote: ... pAK002 (Addgene ID #128215), pAK-EL-02 (Addgene ID #125761 ...
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bioRxiv - Plant Biology 2024Quote: ... pAK-EL-02 (Addgene ID #125761) and pFH66 (Addgene ID #131765 ...
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bioRxiv - Plant Biology 2024Quote: ... pFH51 (Addgene ID #128213), pAK002 (Addgene ID #128215) ...
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bioRxiv - Plant Biology 2024Quote: Designer TALEs were constructed using the Golden Gate TALEN and TALE Kit 2.0 (Addgene, Watertown, MA, USA) according to the manufacturer’s protocol79 ...
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bioRxiv - Plant Biology 2024Quote: ... The vectors pFH38 (Addgene ID #126012), pFH51 (Addgene ID #128213) ...
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bioRxiv - Plant Biology 2024Quote: ... Arabidopsis thaliana DJ-1C (without N-terminal signal sequence) and DJ-1E were cloned into pRS415 (Addgene) using BamHI and SalI sites under GPD (Glyceraldehyde -3-phosphate dehydrogenase ...
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bioRxiv - Plant Biology 2024Quote: ... AtDJ-1C (without N-terminal signal sequence) and AtDJ-1E were cloned into a pRSF-duet vector (Addgene) for protein purification by using primers P5-P8 (Table 1).
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bioRxiv - Neuroscience 2024Quote: Mice were injected with 0.7μl of pGP-AAVrgsyn-jGCaMP7f-WPRE virus (1.85× 1013GC/ml; Addgene) in the nucleus accumbens (NAc ...
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bioRxiv - Neuroscience 2024Quote: ... elegans optimized GFP with rpl-18 intron 2 and 150 bp synthetic PATC introns from pCFJ2412 74 with a floxed unc-119 selection cassette in an intron and a flexible linker from pID3.01 (Addgene 22720) were inserted into the upstream operonic SL2 ATG of tat-5b and tat-5d using pNU2343 ...
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bioRxiv - Neuroscience 2024Quote: ... Each eye was injected with 1μL of AAV2-Ef1a-fDIO-mCherry (7×10¹² vg/mL; Addgene, Catalog #: 114471-AAV2) using a custom Hamilton syringe (Borghuis Instruments ...
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bioRxiv - Plant Biology 2024Quote: ... pICH41421 (Addgene ID #50339) was provided by Sylvestre Marillonnet and Nicola Patron (Earlham Institute ...
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bioRxiv - Plant Biology 2024Quote: ... pICH47742 (Addgene ID #48001), pICH47751 (Addgene ID #48002 ...
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bioRxiv - Plant Biology 2024Quote: ... and pICH41766 (Addgene ID #48018) were provided by Sylvestre Marillonnet (Leibniz Institute of Plant Biochemistry ...
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bioRxiv - Plant Biology 2024Quote: ... pICH47751 (Addgene ID #48002) and pICH41766 (Addgene ID #48018 ...
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bioRxiv - Neuroscience 2024Quote: ... A DNA mix containing the required plasmids (pMD2.G (#12259 Addgene), psPAX2 (#12260 Addgene) ...
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bioRxiv - Microbiology 2024Quote: ... obtained from Addgene, served as an empty vector for cloning purposes ...
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bioRxiv - Neuroscience 2024Quote: The following constructs were used: human CACNA1B (hα1B; variant +e10a, +18a, Δ19a, +e31a, +e37b and +e46; Addgene #62574 ...
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bioRxiv - Molecular Biology 2024Quote: ... pRSV-Rev (Addgene no. 12253), pMD2.G (Addgene no ...
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bioRxiv - Neuroscience 2024Quote: ... Wilson (Addgene plasmid #112865 ...
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bioRxiv - Neuroscience 2024Quote: ... pUltra-chili (Addgene #48687), or pUltra-dox (Addgene #58749 ...
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bioRxiv - Cancer Biology 2024Quote: The FoxO1-mRuby2 construct was cloned into the pLenti-puro lentiviral backbone using Gibson cloning and the pLenti-FoxO1-Clover70 and ERKKTR-mRuby constructs23 (Addgene plasmids nb 67759 & 90231 ...
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bioRxiv - Bioengineering 2024Quote: Heavy and light chain DNA sequences of antibody fragments (Fab) were purchased from Twist Bioscience and cloned individually into the pHLsec mammalian expression vector (Addgene, #99845) using Gibson assembly ...
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bioRxiv - Bioengineering 2024Quote: Primers (Table S1) were used to amplify a ∼500 bp region of β-catenin and TA-cloned into pJC53.2 (Addgene Plasmid ID: 26536) (Collins et al. ...
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bioRxiv - Bioengineering 2024Quote: ... The parental pCT-Con2 vector (Addgene # 41843) was digested with NheI ...
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bioRxiv - Biophysics 2024Quote: The gene fragment corresponding to S-OPA1 (Addgene plasmid ID: 26047; residues 252-960) was subcloned into the pCA528 vector ...
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bioRxiv - Biophysics 2024Quote: ... and γ subunits were gifts from Christie Thomas (Addgene plasmid # 83430 ...
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bioRxiv - Biophysics 2024Quote: Exo70 was amplified from pEGFP-C3-Exo70 (Addgene plasmid #53761) using the primers 5’-CGGAATTCTG ATGATTCCCC CACAGGAGG-3’ and 5’-GCGTCGACAC GGCAGAGGTG TCGAAAAGGC-3’ and was inserted into the pSEMS-HaloTag-vector .
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bioRxiv - Cell Biology 2024Quote: ... and ITSN1-tgRFPt-SspB were PCR-amplified from pLL7.0:Venus-iLID-CAAX (Addgene #60411 ...
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bioRxiv - Cell Biology 2024Quote: ... we used a plasmid that was a gift from Sandra Schmid (Addgene plasmid # 34680; http://n2t.net/addgene:34680; RRID: Addgene_34680)27 ...
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bioRxiv - Cell Biology 2024Quote: The plasmids used for the luciferase assay were generated by cloning 1 kb of either the human HSF2 promoter or MMP9 promoter upstream of the respective TSS into a STARR-seq luciferase validation vector_ORI_empty vector (#99297, Addgene). The DNA from HDFs was isolated by first lysing cells in 3 ml lysis buffer (10 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... lentiCRISPRv2 constructs were independently packaged into lentiviral particles in HEK293T cells with psPAX2 (Addgene cat. 12260) and pMD2.G (Addgene cat ...
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bioRxiv - Cancer Biology 2024Quote: ... into pLenti-CMV/TO-puroR-DEST (Addgene cat. 17293). To regulate expression of the CMV/TO-AXL transcript ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G (Addgene cat. 12259), according to the Lipofectamine 2000 protocol (Invitrogen cat ...
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bioRxiv - Cancer Biology 2024Quote: ... we utilized the lentiCRISPRv2 vector (Addgene cat. 52961) with one of two AXL-targeted sgRNAs (Oligo 1 ...
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bioRxiv - Cell Biology 2024Quote: The PA-mCherry1-N1 vector was a gift from Michael Davidson (Addgene, 54507; http://n2t.net/addgene:54507; RRID: Addgene_54507; deposited by Michael Davidson), and from this we generated a KFERQ-PA-mCherry1 construction composed by the N-terminal 21 amino acids of bovine RNASE1/RNase A containing its KFERQ CMA-targeting motif ...
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bioRxiv - Cell Biology 2024Quote: ... DR274 vector (Addgene #42250) containing the guide RNA sequence was first linearized with Bsa1 ...
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bioRxiv - Cell Biology 2024Quote: The SunTag system plasmids pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro (Addgene plasmid #60909; RRID: Addgene_60909) and pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE (Addgene plasmid #60906 ...
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bioRxiv - Cell Biology 2024Quote: ... twin StrepTagII-TMEM41B and FLAG-TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.
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bioRxiv - Cell Biology 2024Quote: ... The pX602-AAV-Cre sgRNA construct was derived from the pX602 vector (Addgene, 61593) in our previous study43 ...
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bioRxiv - Cell Biology 2024Quote: pAAV-TBG-mCLCC1-StrepTagII-FLAG-StrepTagII was generated from pAAV-TBG-EGFP (Addgene, 105535) by replacing the GFP sequence with the murine Clcc1 cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502).
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bioRxiv - Cell Biology 2024Quote: ... and pMD2.G (Addgene, 12259) plasmids were introduced into the cells using PEI according to the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Oligonucleotides were cloned into either the pX602-AAV-Cre sgRNA backbone for CRISPR/Cas9-induced acute gene knockout in mouse liver or the pLentiCRISPR V2 (Addgene, 52961) & pLentiGuide Blast for genome editing in cell lines ...
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bioRxiv - Cell Biology 2024Quote: ... The pLentiGuide Blast construct was derived from the pLentiGuide Puro vector (Addgene, 52963), in which blasticidin S deaminase was cloned between the BsiWI and MluI restriction sites to replace puromycin N-acetyltransferase.
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bioRxiv - Cell Biology 2024Quote: ... and pMD2.G (Addgene plasmid #12259) lentiviral packaging plasmids were a gift from Didier Trono.
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bioRxiv - Cell Biology 2024Quote: ... psPAX2 (Addgene, 12260), and pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... psPAX2 (Addgene plasmid #12260) and pMD2.G (Addgene plasmid #12259 ...