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Citations for Addgene :
3551 - 3600 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... whereby the pBAD-mTagBFP2 plasmid was a gift from Vladislav Verkhusha (Addgene plasmid no ...
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bioRxiv - Immunology 2024Quote: ... whereby the pBAD-mTagBFP2 plasmid was a gift from Vladislav Verkhusha (Addgene plasmid no. 34632 ; http://n2t.net/addgene:34632 ; RRID:Addgene_34632) (Subach et al. ...
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bioRxiv - Neuroscience 2024Quote: Cre-inducible AAV5-hsyn-DIO-mCherry (Addgene Cat# 50459-AAV5) and AAV5-mCherry-flex-dtA (plasmid was obtained from Addgene Cat# 58536 ...
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bioRxiv - Cell Biology 2024Quote: ... and HA-SQSTM1 (Addgene #280227) transfections ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 injections of 5µl of AAV PHP.S (Addgene, 28306-PHP.S ...
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bioRxiv - Genetics 2024Quote: ... pSpCas9n(BB)-2A-GFP (PX461) was a kind gift from Feng Zhang (Addgene plasmid #48140; https://www.addgene.org/48140/; RRID:Addgene_48140). Chemically competent OneShot TOP10 Escherichia coli (LifeTechnologies ...
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bioRxiv - Immunology 2024Quote: ... and MSCV-mGfi1-IRES-GFP (#91891, Addgene, MA, USA) were co-transfected with viral envelope constructs Gag-Pol and Env into HEK293T cells by calcium-phosphate transfection method ...
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bioRxiv - Cancer Biology 2024Quote: ... with the plasmid of interest (POI) and two plasmids encoding packaging proteins for viral vector (pAX.2 Addgene Plasmid #35002), pMD2.G Addgene Plasmid #12259) ...
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bioRxiv - Cell Biology 2024Quote: ... the CRY2 PHR domain was PCR amplified from pCRY2PHR-mCherryN1 (Addgene plasmid #26866 ...
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bioRxiv - Immunology 2024Quote: ... and pMD2.g (Addgene plasmd #12259) into HEK293T cells using X-tremeGENETM HP (Sigma-Aldrich 6366546001 ...
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bioRxiv - Cell Biology 2024Quote: Plasmids used for mammalian cell expression of Eps15 variants were derived from Eps15-pmCherryN1 (Addgene plasmid #27696 ...
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bioRxiv - Cell Biology 2024Quote: ... into Eps15-pmCherryN1 (Addgene plasmid#27696 ...
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bioRxiv - Neuroscience 2024Quote: ... we used CRISPRi-i3 iPSCs containing a CAG promoter-driven dCas9-BFP-KRAB cassette inserted into the CLYBL safe harbor locus (Addgene #127968). iPSCs were transduced with control gRNA or a previously validated gRNA targeting DRP184 and differentiated as described above.
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bioRxiv - Microbiology 2024Quote: The plasmid encoding the codon-optimized T7 polymerase was obtained from Addgene (plasmid 65974; 34) as was the VSV-G plasmid (pMD2.G ...
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bioRxiv - Molecular Biology 2024Quote: ... ph-p was cloned from the pFastBac plasmid (Addgene #1925), whereas ATRIP was cloned from cDNA generated from RNA extracted from wild type flies.
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bioRxiv - Cell Biology 2024Quote: ... we cloned both amplicons into the pHR-SFFV lentiviral vector (Addgene#79121, (69)) using In-Fusion cloning kit (Takara) ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV5-mCherry-flex-dtA (plasmid was obtained from Addgene Cat# 58536 ...
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bioRxiv - Neuroscience 2024Quote: ... The pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2 plasmid (Addgene #74010) was digested using BsaI (R0535S ...
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bioRxiv - Microbiology 2024Quote: ... we used pcDNA3-GFP-IMP2-2 (Addgene plasmid # 42175 ...
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bioRxiv - Neuroscience 2024Quote: ... EYFP-DRP1 was obtained from Addgene (45160). For GST-purification ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed using a plasmid VCP (wt)-EGFP gifted from Nico Dantuma (Addgene plasmid # 23971, http://n2t.net/addgene:23971; RRID:Addgene_23971) (72) ...
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bioRxiv - Microbiology 2024Quote: ... we used pcDNA3-GFP-IMP2-2 (Addgene plasmid # 42175, https://www.addgene.org/42175/; RRID:Addgene_42175) as template and the HA-coding sequence was included in one of the primer to generate a C-terminally HA-tagged IGF2BP2 ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed using a plasmid VCP (wt)-EGFP gifted from Nico Dantuma (Addgene plasmid # 23971 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the lentivirus GagPol packaging plasmid (psPAX2) all were obtained from Addgene, and were the gifts of Simon Davis ...
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bioRxiv - Molecular Biology 2024Quote: ... we amplified a fragment of the Cas9 gene containing the H840A mutation from the pRS415_pGal-nCas9(H840A) plasmid (Addgene #79616) with primers NcoI_Cas9_F and Cas9_R ...
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bioRxiv - Molecular Biology 2024Quote: ... We then digested this fragment with the SbfI and AscI restriction enzymes and ligated with a fragment from the pRS415_pGal-nCas9(D10A) plasmid (Addgene #79618) digested with the same pair of enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... and pCMV-VSV-G (10 μg, Addgene #8454) along with a pLenti6.2 expression plasmid (10 μg ...
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bioRxiv - Molecular Biology 2024Quote: ... pDONR207 SARS-CoV-2 nsp1 Delta RC (M1, K164A/H165A, Addgene #164522) and pDONR207 SARS-CoV-2 nsp1 R124A/K125A (M2 ...
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bioRxiv - Molecular Biology 2024Quote: ... nsp1 coding sequences were amplified by PCR from pDONR207 SARS-CoV-2 nsp1 (WT, Addgene #141255), pDONR207 SARS-CoV-2 nsp1 Delta RC (M1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and pDONR207 SARS-CoV-2 nsp1 R124A/K125A (M2, Addgene #164523) plasmids using the primers 5-EX-NSP1 and 3-NB-NSP1 and cloned into pEGFP-N1 digested with EcoRI and NotI enzymes ...
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bioRxiv - Molecular Biology 2024Quote: PSuperPuroRetro-1660s(hPOT1)39 (a gift from Christopher Counter, Addgene #11136) was used as a scramble control in both experiments.
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bioRxiv - Molecular Biology 2024Quote: ... a gift by Titia de Lange (Addgene #18002, Addgene #18008), were used to produce lentiviral vectors in HEK 293T cells ...
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bioRxiv - Molecular Biology 2024Quote: ... we used PSuperPuroRetro-1660(hPOT1) (a gift from Christopher Counter, Addgene #11135)39 ...
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bioRxiv - Molecular Biology 2024Quote: pMSP1D1 (Addgene plasmid #20061 ...
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bioRxiv - Molecular Biology 2024Quote: ... and pMSP2N2 (Addgene plasmid #29520; http://n2t.net/addgene:29520; RRID:Addgene_29520) plasmids were a gift from the Sligar lab 29,30,40,41 ...
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bioRxiv - Molecular Biology 2024Quote: ... Kozak sequence was added to RCaMP1h by PCR using RCaMP1h cDNA from pRSET-RCaMP1h plasmid (Addgene plasmid #42874) as the template ...
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bioRxiv - Molecular Biology 2024Quote: ... The sensing domain for citrate comprised residues 4–133 of the CitAP domain from Klebsiella pneumoniae CitA protein (Addgene plasmid #134301). The sensing domain for glucose comprised residues 24–328 of the bacterial D-galactose-binding periplasmic protein (MglB ...
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bioRxiv - Microbiology 2024Quote: The TurboID-V5 gene sequence was obtained from AddGene (#107167). The AgrD1-32 domain consists of an N-terminal domain from amino acid 1-27 and the AIP region from amino acid 28-32 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were co-transfected with 0.25 µg of HA-D1R and 0.25 µg of β-arrestin2-GFP (plasmid # 35411, Addgene, Watertown, MA) in 10 µL of lipofectamine2000 per well as directed by the manufacturer’s protocol and incubated overnight ...
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bioRxiv - Microbiology 2024Quote: ... pCMV-HA-USP18 was generated through subcloning using Flag-HA-USP18 (Addgene #22572; a gift from Wade Harper (87)) as a template.
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bioRxiv - Microbiology 2024Quote: ... pcDNA-V5-NSP3 was generated by amplifying the full-length NSP3 ORF from pDONR207 SARS-CoV-2 NSP3 (Addgene #141257; a gift from Fritz Roth (86)) and by subcloning it into the pcDNA3-C-V5 vector ...
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bioRxiv - Microbiology 2024Quote: ... The gam-gfp was placed under the control of the Tetracycline induction system (Addgene, pFD152, #125546). Similarly ...
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bioRxiv - Microbiology 2024Quote: ... or pTRIPZ (gift from Sandra Demaria – Addgene #127696) vectors using In-Fusion cloning (Takara) ...
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bioRxiv - Microbiology 2024Quote: CMT-93 cells expressing mCherry were created by transfection with pMCB320 (a gift from Michael Bassik, Addgene plasmid #89359 ...
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bioRxiv - Microbiology 2024Quote: ... and psPAX2 (a gift from Didier Trono, Addgene plasmid #12260 ...
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bioRxiv - Microbiology 2024Quote: ... pCMV-VSV-G (a gift from Bob Weinberg, Addgene plasmid #8454, http://n2t.net/addgene:8454, RRID:Addgene_8454), and psPAX2 (a gift from Didier Trono ...
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bioRxiv - Microbiology 2024Quote: ... pCMV-VSV-G (a gift from Bob Weinberg, Addgene plasmid #8454 ...
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bioRxiv - Microbiology 2024Quote: ... 293β5 cells [56] were transfected with LentiCRISPR v2 (a gift from Feng Zhang, Addgene plasmid #52961 ...
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bioRxiv - Microbiology 2024Quote: ... 293β5 cells [56] were transfected with LentiCRISPR v2 (a gift from Feng Zhang, Addgene plasmid #52961, http://n2t.net/addgene:52961, RRID:Addgene_52961), pCMV-VSV-G (a gift from Bob Weinberg ...
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bioRxiv - Microbiology 2024Quote: CMT-93 cells expressing mCherry were created by transfection with pMCB320 (a gift from Michael Bassik, Addgene plasmid #89359, http://n2t.net/addgene:89359, RRID:Addgene_89359) using Lipofectamine 2000 ...