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Citations for Addgene :
2901 - 2950 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... we cloned Hdac3 sgRNA into lentiCRISPR v2 vector (Addgene #52961; Watertown, MA). Seventy-percent confluent Lenti-XTM 293T cells (Takara Bio USA ...
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bioRxiv - Developmental Biology 2024Quote: ... PsPAX2 (Addgene #12260) and PMD2G (Addgene #12259) ...
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bioRxiv - Plant Biology 2024Quote: ... mCherry targeted to Golgi generated previously was used for Golgi marker (Addgene ID 97401, Park et al. 2017). The membrane bound apoplastic marker constructs were generated by inserting a signal peptide of AtChitinase in front of moxVenus yellow fluorescence protein sequences (Baleza et al ...
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bioRxiv - Neuroscience 2024Quote: ... Oertner) or AAV2/1-EF1a-DIO-iChloC-2A-dsRed (5x1013 GC/ml; Addgene plasmid #70762, a gift from T. Margrie). For calcium imaging experiments ...
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bioRxiv - Neuroscience 2024Quote: ... and eGFP were subcloned from AAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG (Addgene, Cat# 85225). AAVs were diluted 1:5 in 1x PBS immediately prior to use ...
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bioRxiv - Molecular Biology 2024Quote: ... The SMASh tag was removed and replaced with 2xHA dTAG derived from Addgene plasmid 9179224 ...
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bioRxiv - Microbiology 2024Quote: 293TN cells were transfected with the lentiviral envelope and packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Microbiology 2024Quote: ... and pMD2.G (Addgene #12259) and lentiviral transfer plasmid pLENTI-puro using TransIT-293 Transfection reagent (Mirus) ...
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bioRxiv - Microbiology 2024Quote: Codon-optimized constructs with a Kozak sequence were subcloned into pLENTI-puro (Addgene #39481) to create MCPyV ST ko/co ...
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bioRxiv - Molecular Biology 2024Quote: ... a 24-well dish was transfected with 250ng of px33050 containing the RBBP6-targeting guide and 250ng of PCR product containing the dTAG degron preceded by a blasticidin or puromycin selection marker (derived from Addgene plasmid 91792 and 9179324). Jetprime (Polyplus ...
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bioRxiv - Microbiology 2024Quote: ... we introduced the p1NIL-melH plasmid with the pGOAL19 cassette (Addgene plasmid number 20190) using the protocol described by Parish and Stoker (47) ...
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bioRxiv - Molecular Biology 2024Quote: ... D9891)-inducible lentiviral expression vector pCW 57.1 (Addgene #41393) by Gibson assembly ...
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bioRxiv - Neuroscience 2024Quote: ... Takeharu Nagai (Addgene http://n2t.net/addgene ...
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bioRxiv - Neuroscience 2024Quote: ... Takeharu Nagai (Addgene http://n2t.net/addgene: plasmid #51957; RRID: Addgene_51957).
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells were cultured in 10-cm dishes and transiently transfected with 9 µg lentiviral plasmid pLV-ER-GFP (Addgene, 80069, a gift from Pantelis Tsoulfas), 8 µg pCMV-dR8.91 ...
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bioRxiv - Cell Biology 2024Quote: The expression plasmids pCDNA5 FRT/TO-NeoR-HA-VPS35 WT and [D620N] were generated by subcloning either pcDNA5D-FRT/TO-HA-VPS35-WT (MRC PPU; DU26467) or pcDNA5D-FRT/TO-HA-VPS35-D620N (MRC PPU; DU26878) into the vector pcDNA5/FRT/TO-neo (Addgene; 41000). Prior to transfection ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded per well of a 6-well plate and co-transfected with 2.8 µg pHAGE-mKeima-LGALS3 (Addgene; 175780), 2.3 µg pPAX2 (Addgene ...
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bioRxiv - Genomics 2024Quote: ... Janina Lewis (Addgene plasmid #191377) and purchased from Addgene111.
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bioRxiv - Genomics 2024Quote: ... was used as a shuttle vector and purchased from Addgene (Watertown, MA)109 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCMV-VSV-G (Addgene #8454), and pRSV-Rev (Addgene #12253 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviral particles were generated by co-transfecting 1.5 µg of total DNA of pMDLg/pRRE (Addgene #12251), pCMV-VSV-G (Addgene #8454) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... [22] and PH(Akt)-Venus was a gift from Narasimhan Gautam (Addgene plasmid #85223 ...
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bioRxiv - Physiology 2024Quote: Mice with cardiac myocyte-specific overexpression of Ffar4 (CM-Ffar4-Tg) were developed using the CAG-CAT system (Plasmid: 53959, Addgene, Watertown, MA, USA).22 Briefly ...
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bioRxiv - Neuroscience 2024Quote: AAV5-hSyn-DIO-EGFP (Addgene 50457-AAV5; Titer: 1.3e12 vg/mL)
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bioRxiv - Evolutionary Biology 2024Quote: ... pMD2.G (gift from Didier Trono, Addgene plasmid # 12259) and psPAX2 (gift from Didier Trono ...
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bioRxiv - Cancer Biology 2024Quote: ... The mAID-mCherry cassette was amplified from Addgene plasmid 72830 (pMK292)45 and mAID-HaloTag was amplified from Addgene plasmid 112852102 ...
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bioRxiv - Cancer Biology 2024Quote: ... PsPAX2 (Addgene 12260), and VSV-G (Addgene 12259) ...
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bioRxiv - Cancer Biology 2024Quote: ... and VSV-G (Addgene 12259). ViralBoost (Alstembio VB100 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2.CAG.GFP (Addgene, #37825-AAV2, titer: 7×10¹² vg/mL) and AAV2.CAG.tdTomato (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... amplified by PCR and cloned into the NotI and AflII sites of the pEN366 backbone (Addgene #156432). All of the constructs were verified by DNA sequence analysis ...
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bioRxiv - Microbiology 2024Quote: ... and pCMV-VSVG (Addgene, #8454), along with the appropriate cargo plasmid using the XtremeGene9 transfection reagent (MilliporeSigma ...
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bioRxiv - Genetics 2024Quote: ... was combined with the human ACE2 CDS (Addgene #1786) and the three polyadenylation signals (b-globin ...
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bioRxiv - Genetics 2024Quote: ... cells were infected with lentiviral particles generated using the vector #52961 (AddGene) and selected with 20ug/ml puromycin for 24-48 h ...
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bioRxiv - Genetics 2024Quote: ... sgRNAs or expression vectors were transfected along with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Genetics 2024Quote: ... and psPAX2 (Addgene #12260) lentiviral vectors which were into HEK293T packaging cells ...
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bioRxiv - Genetics 2024Quote: Two sgRNAs targeting the start and end of the rme-2 coding sequence were in vitro transcribed from a SP6 transcription template amplified from pDD162 (Addgene #47549) using primers P42 (start sgRNA ...
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bioRxiv - Immunology 2024Quote: ... and GFP PCR amplification from plasmid pDSAG (Addgene #62289) respectively ...
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bioRxiv - Immunology 2024Quote: ... 0.54 μg envelope plasmid pMD2.G (Addgene #12259), and 0.99 μg packaging plasmid psPAX2 (Addgene #12260 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The lentiviral backbone is a third-generation vector derived from Addgene #17297.
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of the packaging plasmid (psPAX2, Addgene #12260), and 2 µg of the envelope plasmid (pMD2.G / VSVG ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was PCR-amplified from one of our previously described plasmids (Addgene #132667)36 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The pCMV Blast Dest lentiviral expression vector (Addgene #17451)67 ...
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bioRxiv - Synthetic Biology 2024Quote: ... pYFAC-ubi-M-pyrG (Addgene ID# 184498) and pYFAC-ODC-pyrG were built by isothermal assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... A1510: pMOD_A1510 (#91036, Addgene) CRISPR knockout system in maize ...
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bioRxiv - Synthetic Biology 2024Quote: ... 140 ng pMD2.G (Addgene Plasmid #12259), and 480 ng of pLKO (Addgene Pladmid #10878 ...
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bioRxiv - Synthetic Biology 2024Quote: ... obtained from pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG (Addgene #155305), in the previous cloned pUt-mNF-GFP RMCE plasmid28 (Addgene #199220) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and pcDNA-Cas9-T2A-STOP-TdT (Addgene Plasmid #126425) were ordered from Addgene9 ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid pBEST-OR2-OR1-Pr-UTR1-deGFP-T500 (Addgene 40019) was the kind gift of Vincent Noireaux (The University of Minnesota) ...
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bioRxiv - Synthetic Biology 2024Quote: ... we used one of our previously established plasmids that harbors the wild-type hTdT gene in a pcDNA3.1 backbone (Addgene #126450)36 ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids expressing luciferase under the control of HBV promoters were purchased from Addgene: preC/C_Luc (pHBV_Luc ...