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Citations for Addgene :
2101 - 2150 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: Libraries were cloned as previously described into a CROP-seq-puro-v2 (Addgene #127458) backbone ...
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bioRxiv - Synthetic Biology 2024Quote: ... CPxx synthetic promoters and RBS were either amplified from plasmids acquired from Addgene, from our stocks ...
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bioRxiv - Synthetic Biology 2024Quote: ... The construction pUCmini-iCAP-PHP.S for AAV-PHP.S serotype assembly was kindly gifted by Viviana Gradinaru (Addgene plasmid #103006). For targeted expression of constructs in cardiomyocytes ...
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bioRxiv - Synthetic Biology 2024Quote: ... TNF (pLI_TNF, which was a gift from Veit Hornung, Addgene plasmid #171179)29 ...
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bioRxiv - Synthetic Biology 2024Quote: Ligands were cloned into a pcDNA backbone to confer high expression in HEK293FT cells (Addgene #138749)26 ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL-10 (pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry, which was a gift from Wendell Lim, Addgene plasmid #85430)9 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genes encoding natural transcription factors were sourced from: cJun (pCLXSN-c-JUN, which was a gift from Jin Chen, Addgene plasmid #102758)36 and BATF (pFUW-TetO-BATF ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was a gift from Ralf Kuehn (Addgene plasmid #74285)33 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the sequences encoding for LgBiT and SpyCatcher003 were amplified from the original LgBiT plasmid and sCatch-GFP plasmid (Addgene #18690241), respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... and TGF-β1 (TGFB1_pLX307, which was a gift from William Hahn and Sefi Rosenbluh, Addgene plasmid #98377)30 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Addgene plasmid #102758)36 and BATF (pFUW-TetO-BATF, which was a gift from Filipe Pereira, Addgene plasmid #178451)37 ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was a gift from Michael Elowitz (Addgene plasmid #78099)32 ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was a gift from Feng Zhang (Addgene plasmid #61425)35 ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was modified by our laboratory in previous work (pPD005, Addgene #138749)26 and further modified here to change the antibiotic resistance marker from ampicillin to kanamycin and to add the additional expression cassette containing a CMV promoter ...
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bioRxiv - Systems Biology 2024Quote: ... 1.5 μg of pMD2.G (Addgene #12259) and 18 μl of 2 mg/ml PEI 25000 was mixed in Opti-MEM (Gibco #31985062 ...
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bioRxiv - Systems Biology 2024Quote: ... was created by PCR cloning of 3xHA-MKK6-EE (Addgene, 47579) with BglII–SalI ends and inserting into pBABE puro (Addgene ...
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bioRxiv - Systems Biology 2024Quote: ... pBABE 3xHA-MKK6-EE puro (Addgene, 216791) was created by PCR cloning of 3xHA-MKK6-EE (Addgene ...
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bioRxiv - Systems Biology 2024Quote: ... and cloned into lentiGuide-Puro (Addgene 52963) by the Golden Gate method as described in (https://media.addgene.org/cms/filer_public/3e/e1/3ee1ce9c-99f9-4074-9a28-109d34971471/zhang-lab-sam-cloning-protocol.pdf) ...
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bioRxiv - Systems Biology 2024Quote: ... and pSLIK CVB3 A67G hygro are BSL2 plasmids that cannot be distributed by Addgene and therefore are available upon request.
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bioRxiv - Systems Biology 2024Quote: We established a stable Cas9-expressing line by infecting EpiSC-5 cells with lentiCas9- Blast (Addgene 52962), followed by selection with 5 µg/ml blasticidin (Sigma 15205 ...
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bioRxiv - Systems Biology 2024Quote: pBABE FLAG-IKK2-EE puro (Addgene, 216790) was created by PCR cloning of IKK2-EE (Addgene ...
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bioRxiv - Systems Biology 2024Quote: ... with BclI–MfeI ends and inserting into pBABE puro (Addgene, 1764) digested with BamHI–EcoRI ...
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bioRxiv - Systems Biology 2024Quote: ... with BglII–SalI ends and inserting into pBABE puro (Addgene, 1764) digested with BamHI–SalI ...
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bioRxiv - Neuroscience 2024Quote: Following plasmids were used for transfection pN1-CMV-sDarken (Addgene plasmid #184799 ...
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bioRxiv - Neuroscience 2024Quote: Following plasmids were used for transfection pN1-CMV-sDarken (Addgene plasmid #184799, pN1-CMV-L-sDarken (Addgene plasmid #184800), null-mutant of sDarken (created in house) ...
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bioRxiv - Neuroscience 2024Quote: ... MARK3-V5 (Addgene, 107235), shRNA control (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The oligo pool containing the sgRNA sequences was synthesized by Genewiz (Suzhou, Jiangsu, China) and cloned into lenti-guide-puro (#52963; Addgene, Watertown, MA, USA) as previously described51 ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was a kind gift of Sander Tans (Addgene #195090). The genomic deletion was achieved by following the λRed recombination protocol [75] ...
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bioRxiv - Synthetic Biology 2024Quote: ... which were kind gifts from Johan Paulsson (Addgene plasmid #85525 and #85524). To construct pJP Rep00 ...
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bioRxiv - Cancer Biology 2024Quote: ... pREV (Addgene #115989), and pMD2.g (Addgene #12259 ...
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bioRxiv - Cancer Biology 2024Quote: ... Guide RNAs (gRNAs) were cloned into the pSpCas9n(BB)-2A-GFP (PX461) plasmid (Addgene #48140) and delivered to parental K562 cells by Amaxa nucleofection using the Amaxa Cell line nucleofector kit V (Lonza VCA-1003) ...
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bioRxiv - Cancer Biology 2024Quote: High-titre lentiviral supernatants were produced by transient transfection of 293T/17 cells with second-generation packaging/envelope vectors pRRE (Addgene #12251), pREV (Addgene #115989) ...
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bioRxiv - Neuroscience 2024Quote: pGP-AAV-syn-jGCaMP7c variant 1513-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 105321-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... A 603 bp long nucleotide encoding for cystatin-7 was cloned into the BamHI/EcoRI sites of pAAV-hSyn-EGFP (# 50465, Addgene) to receive the plasmid pAAV-hSyn-Cst7 ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084 ...
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bioRxiv - Neuroscience 2024Quote: pGP-AAV-syn-jGCaMP7c variant 1513-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 105321-AAV1; http://n2t.net/addgene:105321; RRID: Addgene_105321)54 ...
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bioRxiv - Molecular Biology 2024Quote: ... pKI1.1R plasmid (Addgene #85808) was linearized by incubating 1.5 µg of the plasmid with AarI restriction enzyme for 16 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... RfxCas13d were amplified from pY108 (Addgene #84739), pXR001 (#84739) ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10B M.XmnI (Addgene, 114269)34 and plasmid DNA was purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... This plasmid is available from Addgene (#138589). A version of this plasmid encoding the Sav S112F K121Q mutant was used as the starting point for library generation.
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084; http://n2t.net/addgene:51084; RRID: Addgene_51084]), respectively8,34 ...
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bioRxiv - Neuroscience 2024Quote: ... gene fragments of GcaMP6s and P2A were obtained from pGP-CMV-GcaMP6s-WPRE (which was a gift from Douglas Kim & GENIE Project [Addgene plasmid # 40753 ...
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bioRxiv - Neuroscience 2024Quote: ... gene fragments of GcaMP6s and P2A were obtained from pGP-CMV-GcaMP6s-WPRE (which was a gift from Douglas Kim & GENIE Project [Addgene plasmid # 40753; http://n2t.net/addgene:40753; RRID: Addgene_40753]), and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084 ...
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bioRxiv - Synthetic Biology 2024Quote: ... carrying a previously constructed helper plasmid (24) (pDY118A, Figure S1, Addgene ID 182958). pDY118A encodes SsrA-tagged SpCas9 (10 ...
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bioRxiv - Synthetic Biology 2024Quote: ... sgRNAs containing 20-nucleotide (nt) spacer sequences to direct Cas9 cleavage were encoded on donor or guide plasmids constructed from pAM041 (Figure S2, Addgene ID 217969) as described below ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned by ligating annealed oligonucleotide pairs (Supplemental table 5) into the BsmBI-digested pBPK1520 plasmid (BPK1520 was a gift from Keith Joung. Addgene#65777 ...
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bioRxiv - Neuroscience 2024Quote: ... Lentiviral particles were produced in the HEK293T cell line using psPAX2 (12260, Addgene) and pMD2.G (12259 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were transduced with an inducible system Tet-pLKO-puro (21915, Addgene) encoding an shRNA targeting TARDBP sequence (TRCN0000016038 ...
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bioRxiv - Neuroscience 2024Quote: ... and pMD2.G (12259, Addgene) plasmids (both a gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... peGFP-N1-FLAG (Addgene plasmid # 60360).