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2051 - 2100 of 10000+ citations since 2019

• Genome scale CRISPR screens identify actin capping proteins as key modulators of therapeutic responses to radiation and immunotherapy

  Nipun Verma, et al., bioRxiv - Genetics 2024

  Quote: ... which contains a Rosa26 targeting gRNA and the pTLR repair vector (Addgene Plasmid # 64322) into WT ...
• CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens

  Russell T. Walton, Yue Qin, Paul C. Blainey, bioRxiv - Genomics 2024

  Quote: CROPseq-multi entry vectors with Puromycin (Addgene ID 216217), Zeocin (Addgene ID 216218) ...
• CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens

  Russell T. Walton, Yue Qin, Paul C. Blainey, bioRxiv - Genomics 2024

  Quote: ... Zeocin (Addgene ID 216218), and EBFP2-NLS (Addgene ID 216219 ...
• Transcriptional perturbation of LINE-1 elements reveals their cis-regulatory potential

  Yuvia A Pérez-Rico, et al., bioRxiv - Genomics 2024

  Quote: ... Donor plasmids used for knock-ins at the Rosa26 (pEN111) and Tigre locus (pEN366, Addgene #156432), as well as the Rosa26- and Tigre-specific sgRNA-encoding plasmids (pX335-EN479 ...
• Endosomal LRBA regulates the endo-lysosomal pathway

  Viktória Szentgyörgyi, et al., bioRxiv - Immunology 2024

  Quote: ... LAMP1-GFP (Addgene #34831), mCherry-Rab11a (Addgene #55124 ...
• Endosomal LRBA regulates the endo-lysosomal pathway

  Viktória Szentgyörgyi, et al., bioRxiv - Immunology 2024

  Quote: ... pSpCas9(BB)–2A-puro (pX459) (Addgene, #48139).
• Endosomal LRBA regulates the endo-lysosomal pathway

  Viktória Szentgyörgyi, et al., bioRxiv - Immunology 2024

  Quote: ... mCherry was amplified by PCR from the mCherry-Rab4a (Addgene #55125) plasmid and inserted with NEBuilder HiFi Assembly cloning kit (#E5520S ...
• Endosomal LRBA regulates the endo-lysosomal pathway

  Viktória Szentgyörgyi, et al., bioRxiv - Immunology 2024

  Quote: The following commercially available plasmids were obtained: mApple-Rab5a (Addgene #54944), mApple-Rab7a (Addgene #54945) ...
• Crosstalk between the RNA-binding proteins Regnase-1 and -3 shapes mast cell survival and cytokine expression

  Marian Bataclan, et al., bioRxiv - Immunology 2024

  Quote: HEK293T cells were transfected with 3 µg of pmirGLO plasmid containing the 3’UTR of Zc3h12a or Tnf (Addgene plasmid 207127) (7 ...
• Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 protein

  Vijay Singh Bohara, et al., bioRxiv - Microbiology 2024

  Quote: The following plasmids were used in the current study: pEGFP.PKM2 (Addgene #64698) and pEGFP (Addgene #165830) ...
• Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 protein

  Vijay Singh Bohara, et al., bioRxiv - Microbiology 2024

  Quote: The plasmid used in this study pEGFP-PKM2 (Addgene #64698) is a generous gift from Axel Ullrich lab ...
• Crosstalk between the RNA-binding proteins Regnase-1 and -3 shapes mast cell survival and cytokine expression

  Marian Bataclan, et al., bioRxiv - Immunology 2024

  Quote: ... together with the packaging vectors psPAX2 and pMD2.G (Addgene plasmids 12260 and 12259 by Didier Trono ...
• Building Blocks of Understanding: Constructing a Reverse Genetics Platform for studying determinants of SARS-CoV-2 replication

  Marco Olguin-Nava, et al., bioRxiv - Microbiology 2024

  Quote: ... pET-6xHis-SUMO-MarathonRT encoding MarathonRT was a gift from Anna Pyle (Addgene plasmid # 109029 ...
• Building Blocks of Understanding: Constructing a Reverse Genetics Platform for studying determinants of SARS-CoV-2 replication

  Marco Olguin-Nava, et al., bioRxiv - Microbiology 2024

  Quote: ... pET-6xHis-SUMO-MarathonRT encoding MarathonRT was a gift from Anna Pyle (Addgene plasmid # 109029; http://n2t.net/addgene:109029; RRID: Addgene_109029) and purified according to (PMID 29109157) ...
• Site-specific sulfations regulate the physicochemical properties of papillomavirus-heparan sulfate interactions for entry

  Fouzia Bano, et al., bioRxiv - Microbiology 2024

  Quote: Plasmids for sulfatases were from Addgene (#13003 and #13004). Heparan sulfate sulfotransferase genes (in pDONR223 from the ORFeome v8.1 collection (Dharmacon) ...
• The impact of micro-habitat fragmentation on microbial populations growth dynamics

  Dina Mant, Tomer Orevi, Nadav Kashtan, bioRxiv - Microbiology 2024

  Quote: ... coli MG-1655 cells harboring the pEB1-mGFPmut2 plasmid where used throughout the study (pEB1-mGFPmut2 was a gift from Philippe Cluzel, Addgene plasmid #103980; http://n2t.net/addgene:103980; RRID: Addgene_103980)74 ...
• Single-cell image-based genetic screens systematically identify regulators of Ebola virus subcellular infection dynamics

  Rebecca J. Carlson, et al., bioRxiv - Microbiology 2024

  Quote: HeLa-TetR-Cas9 clonal cells used in the genome-wide screen and polyclonal Huh7 cells transduced with 311-Cas9 (Plasmid Addgene #96924) and selected using 10 µg/mL blasticidin for 7 days were used for secondary screening ...
• RNA structures within Venezuelan equine encephalitis virus E1 alter macrophage replication fitness and contribute to viral emergence

  Sarah E. Hickson, Jennifer L. Hyde, bioRxiv - Microbiology 2024

  Quote: A doxycycline-inducible CRISPR/Cas9 expression vector (pSBtet-puro-Cas9-U6) was generated by cloning the Cas9-U6 portion of pX459 (Addgene #62988; [49] into pSBtet-pur (Addgene #60507; [50]). Cas9 was first cloned into pSBtet-pur using the following primers ...
• RNA structures within Venezuelan equine encephalitis virus E1 alter macrophage replication fitness and contribute to viral emergence

  Sarah E. Hickson, Jennifer L. Hyde, bioRxiv - Microbiology 2024

  Quote: A doxycycline-inducible CRISPR/Cas9 expression vector (pSBtet-puro-Cas9-U6) was generated by cloning the Cas9-U6 portion of pX459 (Addgene #62988 ...
• Epstein–Barr Virus BALF0/1 Subverts the Caveolin and ERAD Pathways to Target B-cell Receptor Complexes for Degradation

  Stephanie Pei Tung Yiu, et al., bioRxiv - Microbiology 2024

  Quote: ... or LentiGuide-zeo (a gift from Rizwan Haq, Addgene plasmid #160091 ...
• Epstein–Barr Virus BALF0/1 Subverts the Caveolin and ERAD Pathways to Target B-cell Receptor Complexes for Degradation

  Stephanie Pei Tung Yiu, et al., bioRxiv - Microbiology 2024

  Quote: ... pLenti-spBsmBI-sgRNA-Hygro (a gift from Rene Maehr (Addgene plasmid #62205; http://n2t.net/addgene:62205; RRID:Addgene_62205) (97 ...
• Epstein–Barr Virus BALF0/1 Subverts the Caveolin and ERAD Pathways to Target B-cell Receptor Complexes for Degradation

  Stephanie Pei Tung Yiu, et al., bioRxiv - Microbiology 2024

  Quote: ... respectively (a gift from Robin Ketteler (Addgene plasmid # 122842; http://n2t.net/addgene:122842; RRID:Addgene_122842) (94) ...
• Epstein–Barr Virus BALF0/1 Subverts the Caveolin and ERAD Pathways to Target B-cell Receptor Complexes for Degradation

  Stephanie Pei Tung Yiu, et al., bioRxiv - Microbiology 2024

  Quote: ... sgRNAs were cloned into pLentiGuide-puro (a gift from Feng Zhang, Addgene plasmid #52963; http://n2t.net/addgene:52963; RRID:Addgene_52963)(96) ...
• Epstein–Barr Virus BALF0/1 Subverts the Caveolin and ERAD Pathways to Target B-cell Receptor Complexes for Degradation

  Stephanie Pei Tung Yiu, et al., bioRxiv - Microbiology 2024

  Quote: ... pLenti-spBsmBI-sgRNA-Hygro (a gift from Rene Maehr (Addgene plasmid #62205 ...
• Epstein–Barr Virus BALF0/1 Subverts the Caveolin and ERAD Pathways to Target B-cell Receptor Complexes for Degradation

  Stephanie Pei Tung Yiu, et al., bioRxiv - Microbiology 2024

  Quote: ... 400ng psPAX2 (a gift from Didier Trono, Addgene plasmid #12260; http://n2t.net/addgene:12260; RRID:Addgene_12260) and 150ng VSV-G plasmids for packaging ...
• The segmented flavivirus Alongshan virus reduces mitochondrial mass via degrading STAT2 to suppress innate immune response

  Yinghua Zhao, et al., bioRxiv - Microbiology 2024

  Quote: ... the cDNA of NSP1 were amplified from the Flag-NSP1 template and cloned into the lentiviral vector pWPI (Addgene, Cat# 12254). Plasmid expressing the human STAT2 mutant (HA-STAT2-F175A/R176A ...
• The segmented flavivirus Alongshan virus reduces mitochondrial mass via degrading STAT2 to suppress innate immune response

  Yinghua Zhao, et al., bioRxiv - Microbiology 2024

  Quote: ... along with 3.1 μg of pMD2.G (Addgene, Cat# 12259) and 5.5 μg of psPAX2 (Addgene ...
• DNA damage drives antigen diversification through mosaic VSG formation in Trypanosoma brucei

  Jaclyn E. Smith, et al., bioRxiv - Microbiology 2024

  Quote: ... pLEW100V5-BSD was a gift from George Cross (Addgene plasmid # 27658; http://n2t.net/addgene:27658; RRID:Addgene_27658). pRPaCa9 was a gift from David Horn (Addgene plasmid # 111819 ...
• The nanoscale organization of the Nipah virus fusion protein informs new membrane fusion mechanisms

  Qian Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... AP2μ2-mcherry plasmid is a gift from Christien Merrifield (Addgene # 27672). The HA-tagged NiV-G and GFP-tagged NiV-M were constructed previously.
• DNA damage drives antigen diversification through mosaic VSG formation in Trypanosoma brucei

  Jaclyn E. Smith, et al., bioRxiv - Microbiology 2024

  Quote: ... (Addgene plasmid # 111820; http://n2t.net/addgene:111820; RRID:Addgene_111820)
• DNA damage drives antigen diversification through mosaic VSG formation in Trypanosoma brucei

  Jaclyn E. Smith, et al., bioRxiv - Microbiology 2024

  Quote: ... (Addgene plasmid # 111820 ...
• Transcription factor EB (TFEB) interaction with RagC is disrupted during Enterovirus D68 Infection

  Alagie Jassey, et al., bioRxiv - Microbiology 2024

  Quote: ... The TFEB wild-type (WT-TFEB [plasmid #38119]) and mutant (Δ30-TFEB [plasmid #44445]) constructs were obtained from Addgene (deposited by Shawn Ferguson) and transfected into H1HeLa using the Lipofectamine 2000 transfection reagent ...
• DNA damage drives antigen diversification through mosaic VSG formation in Trypanosoma brucei

  Jaclyn E. Smith, et al., bioRxiv - Microbiology 2024

  Quote: ... pLEW100V5-BSD was a gift from George Cross (Addgene plasmid # 27658 ...
• DNA damage drives antigen diversification through mosaic VSG formation in Trypanosoma brucei

  Jaclyn E. Smith, et al., bioRxiv - Microbiology 2024

  Quote: ... pRPaCa9 was a gift from David Horn (Addgene plasmid # 111819 ...
• DNA damage drives antigen diversification through mosaic VSG formation in Trypanosoma brucei

  Jaclyn E. Smith, et al., bioRxiv - Microbiology 2024

  Quote: ... pRPaCa9 was a gift from David Horn (Addgene plasmid # 111819; http://n2t.net/addgene:111819; RRID:Addgene_111819).
• An expanded genetic toolkit for inducible expression and targeted gene silencing in Rickettsia parkeri

  Jon McGinn, et al., bioRxiv - Microbiology 2024

  Quote: ... pRL0081 was generated from pRL0027 by cloning the Tet-On promoter from pdCas9-bacteria (Addgene catalog number 44249) along with rparr-2 and gfpuv under the control of Tet-ON with a single ompA terminator sequence ...
• ONE HEALTH APPROACH ON SARS-COV-2 – USING SHEEP AS SENTINEL ANIMALS TO INCREASE FUTURE PANDEMIC PREPAREDNESS – a pilot study

  Milena Samojlović, et al., bioRxiv - Microbiology 2024

  Quote: ... BQ.1.1(Addgene #170442 ...
• ONE HEALTH APPROACH ON SARS-COV-2 – USING SHEEP AS SENTINEL ANIMALS TO INCREASE FUTURE PANDEMIC PREPAREDNESS – a pilot study

  Milena Samojlović, et al., bioRxiv - Microbiology 2024

  Quote: ... firefly luciferase reporter (Addgene #170674) and S protein expressing plasmids of different SARS-CoV-2 variants - Wuhan ...
• Krüppel-like Factor (KLF) family members control expression of genes required for serous cavity and alveolar macrophage identities

  Kathleen Pestal, et al., bioRxiv - Immunology 2024

  Quote: ... were transfected with 100ng MSCV plasmid (MSCV multiple cloning site IRES puroR 2A mCherry or GFP) and 10ng pVSVg plasmid (Addgene 8454) with Lipofectamine 2000 (Thermo) ...
• Development of an engineered extracellular vesicles-based vaccine platform for combined delivery of mRNA and protein to induce functional immunity

  Xin Luo, et al., bioRxiv - Immunology 2024

  Quote: ... The OVA entry fragment was created using a PCR amplicon from OVA plasmid (Addgene plasmid 64599) with primers containing attB sites (Table 1 ...
• Human long noncoding RNA, VILMIR, is induced by major respiratory viral infections and modulates the host interferon response

  Kristen John, et al., bioRxiv - Immunology 2024

  Quote: The dCas9-KRAB construct was ordered from Addgene (#89567). The GFP construct for overexpression was constructed from a pSico lentiviral backbone with a bidirectional minimal EF1A-minimal CMV promoter expressing T2A flanked genes ...
• Human long noncoding RNA, VILMIR, is induced by major respiratory viral infections and modulates the host interferon response

  Kristen John, et al., bioRxiv - Immunology 2024

  Quote: ... and pMD2.G (Addgene #12259) using the Lipofectamine 3000 Reagent (Invitrogen) ...
• Human long noncoding RNA, VILMIR, is induced by major respiratory viral infections and modulates the host interferon response

  Kristen John, et al., bioRxiv - Immunology 2024

  Quote: ... psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
• IFNγ-dependent remodelling of the myeloid landscape underlies control of IFNγ-insensitive tumours

  Vivian W.C. Lau, et al., bioRxiv - Immunology 2024

  Quote: ... the single guide RNA (sgRNA) sequences targeting exon 1 or 2 of murine IFNγR1 were cloned into the pX458 backbone (Addgene) containing Cas9 expression and GFP expression ...
• Development of an engineered extracellular vesicles-based vaccine platform for combined delivery of mRNA and protein to induce functional immunity

  Xin Luo, et al., bioRxiv - Immunology 2024

  Quote: ... Plasmids with correct insert were cloned into a lentivirus backbone destination vector (Addgene, plasmid 17451) with LR Clonase II Enzyme Mix (Invitrogen ...
• Development of an engineered extracellular vesicles-based vaccine platform for combined delivery of mRNA and protein to induce functional immunity

  Xin Luo, et al., bioRxiv - Immunology 2024

  Quote: ... followed by transfection with OVA lentivirus transfer vector and lentivirus packaging vectors psPAX2 (Addgene, plasmid 12260) and pMD2.G (Addgene ...
• Development of an engineered extracellular vesicles-based vaccine platform for combined delivery of mRNA and protein to induce functional immunity

  Xin Luo, et al., bioRxiv - Immunology 2024

  Quote: ... and pMD2.G (Addgene, plasmid 12259) using Lipofectamine3000 transfection reagent (Invitrogen ...
• Characterization of the SARS-CoV-2 BA.5 Variants in H11-K18-hACE2 Hamsters

  Mei Dong, et al., bioRxiv - Immunology 2024

  Quote: ... The pK18-hACE2 plasmid was obtained from Addgene (Cat No:149449) (31) ...
• FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria

  Manuel Halte, et al., bioRxiv - Microbiology 2024

  Quote: Plasmid pXY027-ftsZ-eGFP used for visualisation of the Z-ring was a gift from Jie Xiao (Addgene plasmid # 98915)33 ...
• Granzyme B-based CAR T cells block metastasis by eliminating circulating tumor cells

  Bin Sun, et al., bioRxiv - Immunology 2024

  Quote: ... The entire coding sequence of the CAR expression molecule was cloned and inserted into the lentiviral vector pTomo (#26291, Addgene, USA) and expressed under the control of the CMV promoter ...