Labshake search
Citations for Addgene :
9951 - 10000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... A 1.0 uL glass Hamilton syringe was lowered into the brain for infusions of dLight1.1 (pAAV5-CAG-dLight1.1, AddGene #111067-AAV5), which was delivered at a total volume of 0.75uL (0.1uL/min) ...
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bioRxiv - Microbiology 2023Quote: ... pcDNA-RFP was purchased from Addgene.
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bioRxiv - Neuroscience 2023Quote: ... Ntsr1-Cre and Rbp4-Cre mice were stereotaxically injected with an inhibitory opsin (AAV1-hSyn-SIO-stGtACR2-FusionRed, 5 x 1011 vg/ml, Addgene) see “Virus injection and optical fiber implantation” ...
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bioRxiv - Neuroscience 2023Quote: ... an inhibitory opsin (AAV1-hSyn-SIO-stGtACR2-FusionRed, 5 x 1011 vg/ml, Addgene) or a control virus (AAV2-hSyn-DIO-EGFP ...
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bioRxiv - Microbiology 2023Quote: ... in the target plasmid pMB2a-tet (a modified version of Addgene number ...
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bioRxiv - Neuroscience 2023Quote: ... we used AAV1-syn-GCaMP6f-WPRE-SV40 (Addgene viral prep # 100837-AAV1; http://n2t.net/addgene:100837; RRID:Addgene_100837) diluted to a titer of ∼5.0 x 1012 GC/mL with phosphate buffered saline.
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bioRxiv - Neuroscience 2023Quote: ... we used AAV1-syn-GCaMP6f-WPRE-SV40 (Addgene viral prep # 100837-AAV1 ...
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bioRxiv - Cell Biology 2023Quote: ... pEF1α:flex-tdTomato (Addgene: Cat#28306), pCAG:mito-YFP (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were infected with pHIV-Luc-ZsGreen viral particles (Addgene plasmid # 39196), FACS sorted for ZsGreen expression and used for in vivo experiments ...
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bioRxiv - Cell Biology 2023Quote: ... We used plasmids pHage-KDR (#116754) and pHage-puro (#118692) from Addgene with changed resistance gene to hygromycin using a cDNA containing Hygro gene and cloned into ClaI/NdeI and BfuAI/ClaI sites to generate overexpression constructs ...
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bioRxiv - Cancer Biology 2023Quote: ... shRNAs were designed and synthesized from IDT and cloned into Tet-pLKO-puro vector (Addgene plasmid # 21915) according to the previously used protocols 38 ...
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bioRxiv - Cell Biology 2023Quote: ... self-complementary single-stranded DNA oligos (Supp Table S1) were annealed and cloned into AgeI/EcoR1 sites of Tet-pLKO-puro vector (Addgene plasmid # 21915). MSI2 ORF (NM_138962.2 ...
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bioRxiv - Cancer Biology 2023Quote: ... CRISPR/Cas9 sgRNAs were designed and synthesized by IDT and cloned into pLenti-CRISPR V2 (Addgene plasmid # 52961) according to the previous used protocols 39 ...
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bioRxiv - Biochemistry 2023Quote: ... CryC was constructed by inserting PCR amplified spTC (Addgene plasmid #153003, (Cho et al., 2020a)) onto restriction-digested Cry2-mCherry (Addgene plasmid # 26871 ...
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bioRxiv - Biochemistry 2023Quote: CibN was generated by restriction digesting a CIB-pmEGFP plasmid (Addgene plasmid # 28240, (Kennedy et al., 2010)) at AgeI and NheI sites and fusing PCR amplified CIB1 and spTN (Addgene plasmid # 153002 ...
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bioRxiv - Cancer Biology 2023Quote: ... and pCMV-VSV-G (RRID:Addgene_8454) in DMEM containing 10% FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... 100K-200K HEK 293 cells were reverse transfected with the M50 Super 8x TOPFlash plasmid (100 ng; Addgene #12456; gift from Randall Moon) and a construct constitutively expressing Renilla luciferase (5 ng ...
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bioRxiv - Cancer Biology 2023Quote: ... pMDLg/pRRE was a gift from Didier Trono (Addgene plasmid # 12251; http://n2t.net/addgene:12251; RRID:Addgene_12251). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ...
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bioRxiv - Developmental Biology 2023Quote: ... and viral packaging plasmids (psPAX2, Addgene #12260); both gifts from Didier Trono ...
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bioRxiv - Cell Biology 2023Quote: ... and inserted in the pCS2+ plasmid (Addgene) using restriction-enzyme cloning ...
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bioRxiv - Cell Biology 2023Quote: eGFP with three perfect miR430 target sites in its 3’UTR (eGFP-3xPT-miR430b) was inserted in the pCS2+ plasmid (Addgene) as described before 24 ...
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bioRxiv - Cell Biology 2023Quote: ... pCS2TAL3-DD and pCS2TAL3-RR were gifts from David Grunwald (Addgene plasmids # 37275 and 37276; RRID:Addgene_37275; RRID:Addgene_37276). mRNA encoding the TALE nuclease subunits was synthesized using the mMESSAGE mMACHINESP6Transcription Kit (AM1340 ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmids pCS2TAL3-RR-zrif1-exon8 (Addgene plasmid 194434), pCS2TAL3-DD-zrif1-exon8 (Addgene plasmid 194435) ...
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bioRxiv - Cell Biology 2023Quote: ... pCS2TAL3-DD and pCS2TAL3-RR were gifts from David Grunwald (Addgene plasmids # 37275 and 37276; RRID:Addgene_37275; RRID:Addgene_37276). mRNA encoding the TALE nuclease subunits was synthesized using the mMESSAGE mMACHINESP6Transcription Kit (AM1340 ...
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bioRxiv - Cell Biology 2023Quote: ... pCS2TAL3-DD and pCS2TAL3-RR were gifts from David Grunwald (Addgene plasmids # 37275 and 37276 ...
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-PH-OSBP was a gift from Marci Scidmore (Addgene plasmid # 49571 ...
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-PH-OSBP was a gift from Marci Scidmore (Addgene plasmid # 49571 ; http://n2t.net/addgene:49571 ; RRID:Addgene_49571) (Moorhead et al. ...
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bioRxiv - Cell Biology 2023Quote: ... pmCherry-Vinculin was a gift from Chinten James Lim (Addgene plasmid # 80024 ; http://n2t.net/addgene:80024 ; RRID:Addgene_80024) (Lee et al. ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 μg pMD2G (12259; Addgene) using Lipofectamine 2000 according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... transduced with recombinant adeno-associated virus (rAAV) (Addgene, pAAV TBG FFLuc) supernatant ...
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bioRxiv - Immunology 2023Quote: ... LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449; http://n2t.net/addgene:139449; RRID: Addgene_139449). DC 2.4 cells that were negative for surface β2m ...
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bioRxiv - Immunology 2023Quote: ... DC 2.4 cells were transduced using the lentiCas9-Blast vector (Addgene plasmid # 52962; http://n2t.net/addgene:52962; RRID: Addgene_52962) and selected with 10 ug/mL of Blasticidin (AG Scientific #3513-03-9) ...
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bioRxiv - Immunology 2023Quote: ... DC 2.4 cells were transduced using the lentiCas9-Blast vector (Addgene plasmid # 52962 ...
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bioRxiv - Biochemistry 2023Quote: ... UT Health Sciences Center at San Antonio) and insect cell codon-optimized full-length BARD1 (pFastBac-Twin-StrepTagII-BARD11-777; gift from A. Deans, Addgene plasmid #137166)(Tan et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Wolberger (Addgene plasmid #75299) and purified as previously described(Morgan et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Bpa-mutant Pet28n-6xHis-BRCA11-104-(GS)6-BARD126-221 plasmids were co- transformed with a pEVOL-pBpF plasmid encoding an Bpa aminoacyl tRNA synthetase (gift from P. Schultz, Addgene #31190) in E ...
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bioRxiv - Biochemistry 2023Quote: ... fluorophore labelled ‘601’ DNA was generated using large-scale PCR with Phusion polymerase (produced in-house) from a pGEM- 3z/601 plasmid containing one copy of ‘601’ DNA (gift from J. Widom, Addgene plasmid #26656)(Lowary & Widom ...
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bioRxiv - Cell Biology 2023Quote: ... bound to FOXM1-chDNA1 or gRNAs (5’-GCA GGC AGA GCG TAA GCA AA-3’ and 5’-GGA CAC ACG TTT AAT CGA GT-3’) bound to FOXM1-chDNA3 was cloned into the pX335 vector (Addgene, 42335) before the gRNA scaffold ...
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bioRxiv - Cancer Biology 2023Quote: ... SNAIL1 (Addgene #16225[18]) and SLUG (Addgene #25696 ...
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bioRxiv - Cancer Biology 2023Quote: ... sh2: 5-CCAACAACATCTTCTGCTCC-3’ were cloned into the lentiviral vector pLKO.1 (Addgene #8453)[16] ...
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bioRxiv - Cancer Biology 2023Quote: ... and SLUG (Addgene #25696) were fused to GFP-C (Clontech) ...
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bioRxiv - Cell Biology 2023Quote: ... vector while the FBXL4 sgRNA-1 (5’-TTGGTCAGAGAGACCTACGA-3’) and FBXL4 sgRNA-2 (5’-TGGACTACCTCTGCATTGAG-3’) plasmids were cloned into the pLenti-sgRNA (71409; Addgene) vector ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transiently transfected with pSpCas9(BB)-2A-GFP (PX458; 48138; Addgene) vector encoding a sgRNA targeting PAC (5′-TGTCGAGCCCGACGCGCGTG-3′ ...
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bioRxiv - Cell Biology 2023Quote: ... 13.5 μg pPAX2 (12260; Addgene) and 5 μg pMD2G (12259 ...
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bioRxiv - Cell Biology 2023Quote: ... The fragments of bPAC and TGNP were amplified from cytoplasmic-bPAC (a gift from Dr. Reiter lab) and pmApple-TGNP-N-10 (Addgene plasmid #54954), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... CRC PDO were transfected with H2B-RFP (Addgene 26001). CRC CAFs (+GFP ...
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bioRxiv - Cell Biology 2023Quote: ... and CAAX were cloned from pCS2+dnPKA-GFP (a gift from Randall Moon, Addgene #16716), msfGFP containin plasmid (a gift from Dr ...
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bioRxiv - Genetics 2023Quote: ... UASp-dCas:HA:Turbo:NLS was made by combining the dCas9 open reading frame from SID3s-dCas9-KRAB (RRID:Addgene_106399) with HA:TurboID:NLS in the pUASpattB vector (RRID:DGRC_1358) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This construct was placed downstream of the PrhaBAD promoter on the miniTn7 suicide-vector pJM220 (obtained from the Addgene plasmid repository, plasmid #110559) by restriction-ligation and transferred to E ...
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bioRxiv - Cell Biology 2023Quote: ... The pCAG R2pH-LAMP1-3xFLAG plasmid was a gift from Massimiliano Stagi (Addgene plasmid # 157940; http://n2t.net/addgene:157940; RRID: Addgene_157940). The cells used in this study were negatively tested for mycoplasma contamination using MycoAlert™ Mycoplasma Detection Kit (LT07-118 ...