Labshake search
Citations for Addgene :
9751 - 9800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... the pU6-Universal plasmid (Addgene, cat. no. 52694) was digested using the BsaI restriction enzyme then sgRNAs targeting the N terminus of each gene (P1 and P2 for ATPTG8 or P5 and P6 for ATPTG9 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transiently transfected with PX459 (Addgene; #48139) and selected with puromycin for 2 days ...
-
bioRxiv - Cell Biology 2023Quote: ... EJ5-GFP and DR-GFP were purchased from Addgene. p14ARF was cloned into pGEX-4T-1 ...
-
bioRxiv - Cell Biology 2023Quote: Coding sequence of human PITX2C (NM_000325.6) was cloned into lentivirus transfer plasmid (pWPI, Addgene#12254) using Gibson Assembly® kit (NEB ...
-
bioRxiv - Cell Biology 2023Quote: ... pAAV-hSyn1-mNeonGreen (Addgene plasmid 99135) was digested with XbaI and KpnI to release the hSyn1 promoter ...
-
bioRxiv - Cell Biology 2023Quote: ... pGFP–Scrib and PB-TRE-dCas9-VPR (Addgene plasmid # 63800) 44 were digested with BamHI and PmeI ...
-
bioRxiv - Immunology 2023Quote: ... Plasmids encoding ancestral and variant SARS-CoV-2 spike protein were purchased from Addgene (170442 ...
-
bioRxiv - Immunology 2023Quote: ... Plasmids encoding ancestral and variant SARS-CoV-2 spike protein were purchased from Addgene (170442, 170451, 170449, 170450, 172320, 180375; Addgene plasmid, Watertown, Massachusetts, USA).
-
bioRxiv - Immunology 2023Quote: ... psPAX2 and pMD2.G were procured from Addgene.
-
bioRxiv - Immunology 2023Quote: ... and HLA-C0702 encoding cDNA (IDT) were inserted into pSBbi-GP[14] (Addgene plasmid # 60511) using the NEBbuilder HiFi Assembly Master Mix ...
-
bioRxiv - Immunology 2023Quote: ... Stable transfection was performed by transfecting T2 cells with 4.5ug of HLA cDNA in the pSBbi-GP backbone and 0.5ug of pCMV(CAT)T7-SB100[15] (Addgene plasmid # 34879), followed by puromycin selection.
-
bioRxiv - Immunology 2023Quote: ... LentiCRISPRv2–sgRNA Myc transfer plasmids was co-transfected with packaging plasmids psPAX2 and pMD2.G (Addgene plasmids #12259 and #12260) into HEK293T cells ...
-
bioRxiv - Immunology 2023Quote: ... sgRNA targeting Myc was cloned into a Cas9-expressing lentiviral transfer vector (lentiCRISPRv2, # 52961, Addgene, Cambridge, MA) following the methods of the Feng Zhang laboratory (Sanjana et al. ...
-
bioRxiv - Immunology 2023Quote: ... Myc overexpression was achieved using the Nucleofector™ II/2b (Amaxa, Koelin, Germany) with pMXs-cMyc plasmid (Addgene plasmids #13375). Naive CD4+ T cells were resuspended in 100μl nucleofector solution (Amaxa ...
-
The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... DNA encoding the indicated proteins were inserted between the attR recombination sites and shuttled into modified pLEX_307 vectors (Addgene) using Gateway technology (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... and the transgene packaging vector pEGIP-Puro (pEGIP was a gift from Linzhao Cheng, Addgene Plasmid 26777 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The CAT-I gene was amplified from pKD3 (AddGene plasmid #45604). An A to C mutation was made at base pair 219 within the CAT-I gene using the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent ...
-
bioRxiv - Cell Biology 2023Quote: ... pmCherry-Vinculin was a gift from Chinten James Lim (Addgene plasmid # 80024 ...
-
bioRxiv - Cell Biology 2023Quote: ... The CYFIP2-mCherry containing plasmid was a gift from Josef Kittler (Addgene plasmid # 122052). The ECFP-betaPIXa containing plasmid was a gift from Rick Horwitz (addgene #15235) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: CD20 gene (Addgene plasmid # 1890) and pDisplay-LgBiT genes (LgBiT-0L ...
-
bioRxiv - Cell Biology 2023Quote: ... and pBMN-BFP-C1 (Addgene #188644) was previously described (Nguyen et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... pBMN-HA-C1 (Addgene #188645) and pBMN-BFP-C1 (Addgene #188644 ...
-
bioRxiv - Cell Biology 2023Quote: ... pBMN-mEGFP-NDP52 (Addgene # 188785), pBMN-BFP-Parkin (Addgene #186221 ...
-
bioRxiv - Cell Biology 2023Quote: ... and universal vector backbones pBMN-mEGFP (Addgene #188643), pBMN-HA-C1 (Addgene #188645 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Nphs2 was amplified from adult mouse kidney cDNA and cloned into the MCS-13X Linker-BioID2-HA plasmid (MCS-13x Linker-BioID2-HA was a gift from Kyle Roux (Addgene plasmid # 80899 ; http://n2t.net/addgene:80899 ; RRID:Addgene_80899).30 A donor vector was subsequently constructed from the Nphs2-13x Linker-BioID2-HA plasmid with the following features ...
-
bioRxiv - Genomics 2023Quote: TKOv3 library lentivirus was produced by cotransfection of lentiviral vectors psPAX2 (packaging vector; Addgene #12260) and pMDG.2 (envelope vector ...
-
bioRxiv - Genetics 2023Quote: ... the T7 promoter sequence was added to the sgRNA template by PCR amplification using pX335 (Addgene #42335) with Phusion high-fidelity DNA Polymerase Kit (NEB) ...
-
bioRxiv - Cell Biology 2023Quote: ... and introduced into BbsI-linearised pSpCas9(BB)-2A-GFP vector (a gift from Feng Zhang; Addgene # 48138) using Gibson Cloning kit (New England Biolabs) ...
-
bioRxiv - Neuroscience 2023Quote: ... we performed an additional control experiment in which we injected a cre-dependent mCherry virus (pAACV-hSyn-DIO-mCherry; a gift from Bryan Roth; Addgene plasmid #50459 ...
-
bioRxiv - Cell Biology 2023Quote: ... pAAV-CAG-H2B tdTomato was obtained from Addgene (Plasmid#116870, (83)) ...
-
bioRxiv - Neuroscience 2023Quote: ... we performed an additional control experiment in which we injected a cre-dependent mCherry virus (pAACV-hSyn-DIO-mCherry; a gift from Bryan Roth; Addgene plasmid #50459; http://n2t.net/addgene:50459; RRID:Addgene_50459) into the basal forebrain of 3 ChAT-cre mice ...
-
bioRxiv - Systems Biology 2023Quote: We performed three Golden Gate cloning reactions to ligate the CRISPRi inserts into three distinct barcoded CRISPRi vectors (pCRISPR3, AddGene #: 191856-191861). The reaction mix contained 75 ng of barcoded vector ...
-
bioRxiv - Systems Biology 2023Quote: We constructed the third-order sgRNA library in a single barcoded vector (pCRISPR3, AddGene #: 191856-191861) using Golden Gate cloning as described previously with minor modifications ...
-
bioRxiv - Biophysics 2023Quote: The pET28a-EGFP-CNA35 DNA construct purchased from Addgene was described before(Aper et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3.7 μl of 20 mg/ml stock of homemade T7 RNA polymerase (Addgene plasmid p6XHis-T7-P266L33 ...
-
bioRxiv - Molecular Biology 2023Quote: ... generated with pFastBac1-hDicer (Addgene, #89144), and Bac-to-Bac expression system (Gibco) ...
-
bioRxiv - Systems Biology 2023Quote: ... pNBU2_erm-TetR-P1T_DP-GH023 was a gift from Andrew Goodman used for creating the tetracyline-inducible recombinase strain (Addgene plasmid # 90324 ...
-
bioRxiv - Systems Biology 2023Quote: ... pNBU2_erm-TetR-P1T_DP-GH023 was a gift from Andrew Goodman used for creating the tetracyline-inducible recombinase strain (Addgene plasmid # 90324; http://n2t.net/addgene:90324; RRID:Addgene_90324). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: Ntsr1-Cre mice (n = 3) were stereotaxically injected with an inhibitory opsin (AAV1-hSyn-SIO- stGtACR2-FusionRed, 5 x 1011 vg/ml, Addgene) see “Virus injection and optical fiber implantation” 6 weeks prior to the recordings ...
-
bioRxiv - Neuroscience 2023Quote: ... or a control virus (AAV2-hSyn-DIO-EGFP, 100 µL at titer ≥ 3×10¹² vg/mL, Addgene). A subset of the optogenetic L6-CT experiments was done in Ntsr1-Cre-ChR2-EYFP mice ...
-
bioRxiv - Neuroscience 2023Quote: ... A similar approach was used to target SpVc where we injected 500 nL of pAAV5-Syn-Chronos-GFP (Addgene # 59170) at a depth of 0.5 mm ...
-
bioRxiv - Neuroscience 2023Quote: We targeted the A2 region of the cNTS by making a small incision in the meninges over the foramen magnum and injected 500 nL of pAAV5-Syn-FLEX-rc[ChrimsonR-tdTomato] (Addgene # 62723) at a depth of 0.5 mm ...
-
bioRxiv - Cell Biology 2023Quote: ... Lenti-V2-Blast (lentiCRISPR v2-Blast plasmid, Addgene) plasmid was used here ...
-
bioRxiv - Biochemistry 2023Quote: ... and TAF15 were amplified from the plasmids of the wtTDP43– tdTOMATOHA (Addgene, plasmid #28205), GST–TEV–FUS (Addgene ...
-
bioRxiv - Biochemistry 2023Quote: ... the Xlone–GFP (Addgene, plasmid #96930) plasmid was double digested with SpeI and KpnI restriction enzymes ...
-
bioRxiv - Biochemistry 2023Quote: ... pDEST_TAF15 (Addgene, Plasmid #26379), respectively ...
-
bioRxiv - Biochemistry 2023Quote: ... The human Archease gene spanning residues 27-168 was inserted into the 2M-T (Addgene: 29708) plasmid using ligation-independent cloning (LIC) ...
-
bioRxiv - Biochemistry 2023Quote: ... plasmid and the 438-Rgfp (Addgene: 55221) plasmid ...
-
bioRxiv - Biochemistry 2023Quote: ... The human RTCB gene was inserted into the 438B (Addgene: 30115) plasmid and the 438-Rgfp (Addgene ...
-
bioRxiv - Systems Biology 2023Quote: ... pol and rev genes (psPAX2, Addgene), and an envelope plasmid containing VSV-G (pMD2.G ...