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Citations for Addgene :
9701 - 9750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Addgene plasmid pCMV-T7-EGFP (BPK1098) (Addgene plasmid # 133962 ...
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bioRxiv - Bioengineering 2023Quote: ... and subcloned into a bacterial expression vector (Addgene plasmid #29656 ...
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bioRxiv - Bioengineering 2023Quote: ... plasmids expressing LbCas12a and AsCas12a were obtained from Addgene (a gift from Zhang lab and Doudna lab) and directly used for protein expression ...
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bioRxiv - Bioengineering 2023Quote: ... a plasmid containing the human codon-optimized Cas12a gene was obtained from Addgene, then was PCR amplified using Q5 Hot Start high fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... n×GCN4 sequences were derived from plasmid (Addgene #113022) via PCR ...
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bioRxiv - Bioengineering 2023Quote: ... pBbS2k-RFP was a gift from Jay Keasling (Addgene plasmid # 35330 ...
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bioRxiv - Bioengineering 2023Quote: ... near a Cas9 fused tracrRNA-crRNA fusion to replace the RFP and LacI genes in a PCR’ed fragment of pBbS2K-RFP (Addgene #35330). The Golden Gate assembly cassettes of the resulting plasmid (called pSECRETS-B ...
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bioRxiv - Bioengineering 2023Quote: ... pBbS2k-RFP was a gift from Jay Keasling (Addgene plasmid # 35330 ; http://n2t.net/addgene:35330 ; RRID:Addgene_35330) To clone pSECRETS-B derivatives containing off-target sequences and standard gRNAs or x-gRNA libraries) ...
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bioRxiv - Bioengineering 2023Quote: ... the gRNA cassette for aTc-induced expression was constructed by inserting via HiFi Assembly a PCR’ed fragment of TetR from pBbA2c-RFP (Addgene #35326) and a gBlock (IDT ...
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bioRxiv - Bioengineering 2023Quote: ... pBbA2c-RFP was a gift from Jay Keasling (Addgene plasmid # 35326 ; http://n2t.net/addgene:35326 ; RRID:Addgene_35326).
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bioRxiv - Bioengineering 2023Quote: ... pwtCas9-bacteria was a gift from Stanley Qi (Addgene plasmid # 44250 ...
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bioRxiv - Bioengineering 2023Quote: ... ssAAV genomes employed were pAAV:CAG-mNeonGreen (Addgene ID: 99134) and pAAV:CAG-2xNLS-EGFP (equivalent version with one NLS ...
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bioRxiv - Bioengineering 2023Quote: ... into a PCR-ed fragment of plasmid pBbA2c-RFP (Addgene #35326) to replace the red fluorescent protein (RFP ...
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bioRxiv - Bioengineering 2023Quote: ... p11-LacY-wtx1 was a gift from Huimin Zhao (Addgene plasmid # 69056 ...
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bioRxiv - Neuroscience 2023Quote: AnkyrinG-190-GFP was a gift from Vann Bennett (Addgene plasmid # 31059; http://n2t.net/addgene:31059; RRID:Addgene_31059). AnkG-mCherry was a gift from Benedicte Dargent (Addgeneplasmid #42566 ...
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bioRxiv - Cell Biology 2023Quote: ... elegans cDNA library and cloned into UC Berkeley Macrolab vector 2-CT (Addgene #29706), which encodes a TEV protease-cleavable His6-MBP (maltose binding protein ...
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bioRxiv - Developmental Biology 2023Quote: ... A mixture of 12 μg of the validated retroviral vectors pSIREN_EGFP-siLuc (Addgene #122287) 21 expressing GFP under the control of PGK promoter and 9 μg of a VSVG plasmid ...
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bioRxiv - Neuroscience 2023Quote: AnkyrinG-190-GFP was a gift from Vann Bennett (Addgene plasmid # 31059 ...
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bioRxiv - Cell Biology 2023Quote: Day 5 moDCs previously transfected with either NT or gal9 siRNA were transfected with 2 ug of the Str-KDEL_TNFα_SBP_EGFP plasmid (Addgene, #65278) (Boncompain et al ...
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bioRxiv - Neuroscience 2023Quote: ... 2.3 x 1013 vg/mL) or 200 nL/side AAV5-CMV-HI-eGFP-Cre-WPRE-SV4 (Addgene; ≥ 1 x 1013 vg/mL) was injected at a rate of 100 nL/min into the insula of Pdynlox/lox and Oprk1lox/lox mice ...
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bioRxiv - Biochemistry 2023Quote: pMD2.G lentivirus envelope plasmid (Addgene ID #12259)
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bioRxiv - Biochemistry 2023Quote: psPAX2 lentivirus packaging plasmid (Addgene ID #12260)
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bioRxiv - Biochemistry 2023Quote: ... PDZ2 or PDZ1+2 tandem were obtained from Addgene (Tonikian et al., 2008) or purchased from GeneScript respectively and transformed into E ...
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bioRxiv - Biochemistry 2023Quote: Restriction-free cloning 30 was used to generate the constructs of MglA-link-MglB with C-terminal hexahistidine tag in pHis17-KanR (mglA-link-mglB-H6, refer Addgene plasmid #78201 for vector backbone ...
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bioRxiv - Biochemistry 2023Quote: The roGFP-iL gene was purchased from Addgene (Watertown, MA, USA) in a pQE30 plasmid harboring an ampicillin resistance gene ...
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bioRxiv - Biochemistry 2023Quote: ... each pLJM1 plasmid was co-transfected with psPAX2 (Addgene plasmid #12260) and pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Biochemistry 2023Quote: ... corresponding sequences were subcloned into the pLJM1-EGFP vector (Addgene plasmid #19319). To express the YTH domain of human YTHDC1 in E ...
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bioRxiv - Biochemistry 2023Quote: ... and pMD2.G (Addgene plasmid #12259) using Lipofectamine 3000 (Invitrogen L3000015 ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin WT was a gift from Rachel Klevit (Addgene plasmid # 12647; http://n2t.net/addgene:12647; RRID:Addgene_12647) (Brzovic et al. ...
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bioRxiv - Biochemistry 2023Quote: ... UbcH5A was a gift from Cynthia Wolberger (Addgene plasmid # 61081 ...
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bioRxiv - Biochemistry 2023Quote: ... primary cortical neurons were transduced with AAV encoding Cre recombinase (AAV-hSyn-Cre, Addgene 105553-AAV1) by replacing half of the volume of media in the well with media containing the AAV (final virus dilution 1:500 in 200 μl per well) ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid to produce lentiviral particles for the expression of the cAMP BRET biosensor Nluc-EPAC-VV in neurons was generated by amplifying the sequence from pcDNA3.1-Nluc-EPAC-VV and inserting it between the AgeI and EcoRI sites of pLenti-hSynapsin-Cre-WPRE (Addgene #86641;(Sakurai et al., 2016)) by Gibson assembly.
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bioRxiv - Biochemistry 2023Quote: ... pBluescript (Addgene) empty plasmid (180 ng/μl ...
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bioRxiv - Biochemistry 2023Quote: ... pPD95_79 and pPD95_81 kindly provided by Andrew Fire (pPD95_79 - Addgene plasmid #1496; http://n2t.net/addgene:1496; RRID:Addgene_1496and pPD95_81 - Addgene plasmid #1497 ...
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bioRxiv - Biochemistry 2023Quote: ... pPD95_79 and pPD95_81 kindly provided by Andrew Fire (pPD95_79 - Addgene plasmid #1496; http://n2t.net/addgene:1496; RRID:Addgene_1496and pPD95_81 - Addgene plasmid #1497 ...
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bioRxiv - Biochemistry 2023Quote: ... UbcH5A was a gift from Cynthia Wolberger (Addgene plasmid # 61081; http://n2t.net/addgene:61081; RRID:Addgene_61081) (Wiener et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin WT was a gift from Rachel Klevit (Addgene plasmid # 12647 ...
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bioRxiv - Biochemistry 2023Quote: ... Ube1/PET21d was a gift from Cynthia Wolberger (Addgene plasmid # 34965; http://n2t.net/addgene:34965; RRID:Addgene_34965) (Berndsen and Wolberger ...
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bioRxiv - Biochemistry 2023Quote: ... The codon optimized AOX with a C-terminal HA tag synthesized by IDT was cloned into pLv-EF1a-IRES-puro (Addgene 85132), pAAV.TBG.PI.eGFP.WPRE.bGH (Addgene 105535) ...
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bioRxiv - Biochemistry 2023Quote: ... pAAV.TBG.PI.eGFP.WPRE.bGH (Addgene 105535), and pAdv-MCS-IRES-GFP (Vector Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... pET21a-BirA was a gift from Alice Ting (Addgene plasmid # 20857)[100] ...
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bioRxiv - Biochemistry 2023Quote: ... The sense guide insert was subsequently cloned into BbsI digested pBABED P U6 (University of Dundee DU48788) and the antisense cloned into BbsI digested pX335 (Addgene #42335), yielding clones DU69746 and DU69747 respectively.
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bioRxiv - Biochemistry 2023Quote: ... MEFs were split into 6 well plates to ∼80% confluence and transfected with 2 µg SV40 1: pBSSVD2005 (a gift from David Ron; Addgene plasmid #21826) using FuGENE HD according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and psPAX2 and pMD2.G packaging systems (gracious gifts from Didier Trono: Addgene plasmids 12260 and 12259 respectively) were eluted into TE elution buffer ...
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bioRxiv - Biochemistry 2023Quote: The Pfget19_Ulp1 plasmid (Addgene) containing a His6-tagged Ulp1 gene was transformed into T7 Express competent cells (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... mice were injected via the tail vein with 2.5×1011 GC of AAV-TBG-Cre (Addgene, 107787-AAV8) in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... lentiviral plasmids and packaging plasmids (pMD2.5G, Addgene catalog no ...
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bioRxiv - Biochemistry 2023Quote: ... lentiviral plasmids and packaging plasmids (pMD2.5G, Addgene catalog no. 12259 and psPAX2, Addgene catalog no. 12260) were transfected into HEK293T cells using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... smegmatis mc2 155 pTEC27 (Takaki et al., 2013) (a gift from Dr. Lalita Ramakrishnan: Addgene plasmid #30182 ...
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bioRxiv - Biochemistry 2023Quote: WT S100A1 and its variants were synthesized (Eurofins MWG) and cloned into the pAdTrack-CMV plasmid (#16405, Addgene) (Table S1) ...