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Citations for Addgene :
9851 - 9900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid pCX-HO1-2A-EGFP was ordered from Addgene. Goat-anti-Mouse IgG+IgM (10 nm gold ...
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bioRxiv - Biochemistry 2023Quote: ... TgKS5 was cloned into a pMAL-c2X expression vector (kind gift of Paul Riggs, Addgene #75286) containing an N-terminal maltose binding protein (MBP ...
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bioRxiv - Biochemistry 2023Quote: pCAG-EGFP or pCX-HO1-2A-EGFP (Addgene, #74672) were transfected using a standard Lipofectamine 2000 protocol ...
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bioRxiv - Biochemistry 2023Quote: ... coli codon optimized TgTR was cloned into a pMAL-c2X expression vector (kind gift of Paul Riggs, Addgene #75286) containing an N-terminal maltose binding protein (MBP ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) with pACYC-GroES/EL-TF (kind gift of Karl Griswold, Addgene #83923) containing GroES/EL and Trigger Factor protein folding chaperones ...
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bioRxiv - Biochemistry 2023Quote: Mass spectrometry sample preparation: N2A cells were transfected with pcDNA3 plasmids containing 2X myc-EXOSC3 or pcDNA3 empty vector (Addgene) using Lipofectamine® 2000 (Thermofisher ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid pCMV-R-GECO1 (Addgene Plasmid # 32444) was a gift from Robert E ...
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bioRxiv - Cancer Biology 2023Quote: ... the fragment was ligated into pENTR2B (Addgene) and then recombined into pCW57.1 (Addgene) ...
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bioRxiv - Cell Biology 2023Quote: ... Zhang (Addgene plasmid 52961)71 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then recombined into pCW57.1 (Addgene). The empty vector was pCW57.1 alone ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was produced in 293 cells by co-transfecting the lentiviral constructs with pCMV-dR8.2 (Addgene plasmid #8455) and pCMV-VSV-G (Addgene plasmid #8454 ...
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bioRxiv - Cancer Biology 2023Quote: ... and pCMV-VSV-G (Addgene plasmid #8454) viral packaging constructs ...
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bioRxiv - Cell Biology 2023Quote: ... The pMaCTag-P26 plasmid containing the mClover coding region along with a mini-AID was used (Addgene #120037). The online oligo design tool www.pcr-tagging.com was used for searching PAM sites and to design the forward (M1 ...
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bioRxiv - Developmental Biology 2023Quote: Srfp2 cDNA was synthesized (Azenta) and cloned into pR26 CAG/T2AGFP Asc (pR26 CAG/GFP Asc was a gift from Ralf Kuehn, Addgene plasmid # 74285 ...
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bioRxiv - Developmental Biology 2023Quote: ... a generous gift from Bob Weinberg (Addgene plasmid #1780; http://n2t.net/addgene:1780; RRID:Addgene_1780); 4 µg of a plasmid containing the genes for Moloney murine leukemia virus gag-pol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the mouse synaptophysin-mRuby fusion protein was cloned into the generated synapsin:eGFP transgene from AAV-FLExloxP-mGFP-2A-synaptophysin-mRuby (Addgene Plasmid# 71760) plasmid vector courtesy of Liqun Luo (Beier et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... a generous gift from Bob Weinberg (Addgene plasmid #1780 ...
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bioRxiv - Biochemistry 2023Quote: ... The DNMT3A2E756A gene was synthesized by IDT and cloned into pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40 (Addgene #186968).
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bioRxiv - Biochemistry 2023Quote: ... The eHIV enzyme was expressed and purified from pET30-RT1306 (gift from Bryan Dickinson; Addgene plasmid # 131521) following published protocols (40).
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bioRxiv - Genetics 2023Quote: ... the product was cloned using Gibson assembly into lentivector (Addgene #67974) cut with XbaI and XhoI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This was obtained from the pJM230 plasmid69 (obtained from the Addgene plasmid repository, plasmid #110560). Overnight cultures of each strain were set up in biological triplicate ...
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bioRxiv - Microbiology 2023Quote: ... PEL cell lines expressing lentiCas9-Blast (Addgene, 52962) were previously generated and validated by STR profiling7 ...
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bioRxiv - Cell Biology 2023Quote: ... a pDEST-HisMBP (Addgene, #11085) vector containing aa1-255 of Cnn was digested with KpnI and SspI and a complementary fragment containing the point mutations was cloned into the cut vector using HiFi technology (NEB) ...
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bioRxiv - Biochemistry 2023Quote: The expression construct of pET23b-Casp3-His6 was extracted from DH5α bacterial culture purchased from Addgene (Plasmid #11821). BL21-CodonPlus (DE3)-RIPL competent cells were transformed with the expression construct and plated on LB-agar supplemented with ampicillin (100 µg/ml ...
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bioRxiv - Biochemistry 2023Quote: The construct for E.coli expression of WT caspase-3 (pET23b-Casp3-His) was obtained from Addgene (plasmid 11821). The construct for mammalian expression of caspase-3/GFP was a gift from Dr ...
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bioRxiv - Biochemistry 2023Quote: ... Sortase5M (sortase A pentamutant) was a gift from David Liu (Addgene plasmid # 75144), expressed in Arctic Express (DE3 ...
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bioRxiv - Bioengineering 2023Quote: ... The gRNA duplex was cloned into lentiCRISPRv2 (Addgene #52961) (69) ...
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bioRxiv - Bioengineering 2023Quote: PC3-luc cells were derived from PC3 cells transfected with the firefly luciferase expressing plasmid from Addgene (Ubc.Luc.IRES.Puro, RRID:Addgene 33307) (22) ...
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bioRxiv - Bioengineering 2023Quote: PC3-luc cells were derived from PC3 cells transfected with the firefly luciferase expressing plasmid from Addgene (Ubc.Luc.IRES.Puro, RRID:Addgene 33307) (22) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Geir Mathiesen (Addgene plasmid # 122030) (Mathiesen et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... Chris Barnes (Addgene plasmid # 133306) (Fedorec et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... Lars Axelsson (Addgene plasmid # 122028) (Sørvig et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... Mikhail Shapiro (Addgene plasmid # 86116) (Piraner et al. ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into SpeI and SwaI digested p201N Cas9 plasmid (Addgene #59175). E ...
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bioRxiv - Plant Biology 2023Quote: A pair of oligos containing the gRNA sequence were used in conjunction with vector-specific primers (Table S1) for PCR amplification of Medicago truncatula U6 promoter and scaffold from the pUC-gRNA plasmid (Addgene #47024) using Q5® High-Fidelity 2X Master Mix (New England Biolabs) ...
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bioRxiv - Physiology 2023Quote: ... containing human Fis1 gene were purchased from Addgene. The working viral vectors were constructed from these two plasmids by Custom DNA Constructs ...
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bioRxiv - Physiology 2023Quote: ... containing endothelial specific-promoter (CDH5, VE-Cadherin) and green fluorescent protein (GFP) and the plasmid pC4-RhE-FRB-Fis1 (Addgene Cat# 68056) containing human Fis1 gene were purchased from Addgene ...
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bioRxiv - Physiology 2023Quote: Plasmid CDH5-AbR-GFP (Addgene Cat# 122970) containing endothelial specific-promoter (CDH5 ...
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bioRxiv - Genetics 2023Quote: The N- and C-terminally TurboID-tagged constructs were generated by cloning the coding region of kdm5 upstream or downstream of HA:TurboID from pCDNA3-TurboID (RRID:Addgene_107171)(46 ...
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bioRxiv - Microbiology 2023Quote: Library virus was generated and titrated as described above using the Brunello sgRNA pool library in lentiGuide-Puro (Addgene, 73178). In all cases ...
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bioRxiv - Microbiology 2023Quote: ... pLenti SpBsmBI sgRNA Hygro (Addgene, 62205) constructs were cloned by ligating annealed oligonucleotides specified in Table S5C with the BsmBI-digested vector 78.
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bioRxiv - Microbiology 2023Quote: ... while long-term single-gene knockout cell lines were generated using sgRNAs in pLenti SpBsmBI sgRNA Hygro (Addgene, 62205) containing a hygromycin resistance cassette ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were co-transfected with pMD2.G and pPAX2 (Addgene) together with pLKO-shRNA constructs (PLKO.1 shRNA PP4C ...
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bioRxiv - Neuroscience 2023Quote: ... Cal-ID and cpTurboID transgenes were cloned into the pSBtet-Hyg (Addgene #60508) donor vector ...
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bioRxiv - Neuroscience 2023Quote: Cal-ID or cpTurboID transgenes were cloned into pJW1511 (Addgene #62365) or FUGW (Addgene #14883 ...
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bioRxiv - Neuroscience 2023Quote: ... or FUGW (Addgene #14883) transfer vectors after removing the original transgene ...
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bioRxiv - Neuroscience 2023Quote: ... mixed at a 5:1 ratio with pCMV(CAT)T7-SB100 (Addgene #34879), and co-transfected to HEK293T cells using TransIT-293 (Mirus) ...
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bioRxiv - Neuroscience 2023Quote: ... and the appropriate rep/cap plasmid (“rAAV2-retro helper” (Addgene 81070) or pAAV-RC1 (Cellbiolabs VPK-421)) ...
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bioRxiv - Neuroscience 2023Quote: pAAV-syn-FLEX-tTA (Addgene 178516) was made by cloning a codon-optimized tet transactivator gene76 into pAAV-synP-FLEX-EGFP-B19G (Addgene 59333).
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bioRxiv - Neuroscience 2023Quote: ... and pAAV-syn-mCre (Addgene 178515) were made by replacing the FLEX cassette from pAAV-synP-FLEX-EGFP-B19G (Addgene 59333 ...