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• Production of Infectious Reporter Murine Norovirus by VP2 trans-Complementation

  Ryoka Ishiyama, et al., bioRxiv - Microbiology 2023

  Quote: ... was constructed from MSCV IRES GFP vector (Addgene #20672) by inserting the MNV VP2 coding region proximal to the internal ribosome entry site (IRES) ...
• Production of Infectious Reporter Murine Norovirus by VP2 trans-Complementation

  Ryoka Ishiyama, et al., bioRxiv - Microbiology 2023

  Quote: ... and pRSV-Rev (Addgene), into 293T cells ...
• Neuronal FOS reports synchronized activity of presynaptic neurons

  Margarita Anisimova, et al., bioRxiv - Neuroscience 2023

  Quote: ... and AAV-hsyn- CheRiff-eGFP (Addgene #51697) (52 ...
• A histone methylation-MAPK signaling axis drives durable epithelial-mesenchymal transition in hypoxic pancreas cancer

  Brooke A. Brown, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Didier Trono (Addgene plasmid #12260 and #12259 ...
• Revisiting the role of cAMP in Drosophila aversive olfactory memory formation

  Takashi Abe, et al., bioRxiv - Neuroscience 2023

  Quote: ... The amplified fragment was then integrated into the 20xUAS vector originating from pJFRC7-20xUAS-IVS-mCD8::GFP (Pfeiffer, Addgene plasmid #26220) by In-Fusion recombinase (Clontech Laboratories) ...
• Experience-dependent information routing through the basolateral amygdala

  Pantelis Antonoudiou, et al., bioRxiv - Neuroscience 2023

  Quote: ... Mice were stereotaxically injected with 250nl of AAVrg-hSyn1-GCaMP6f-P2A-nls-dTomato (Addgene #51085-AAVrg) into the NAcc (AP ...
• Distinct brain-wide presynaptic networks underlie the functional identity of individual cortical neurons

  Ana R. Inácio, et al., bioRxiv - Neuroscience 2023

  Quote: ... we injected rAAV5-Syn-Flex-GCaMP6f-WPRE-SV40 (Addgene 100833) in the right hemisphere wS1 of Emx1-IRES-Cre mice (in mm relative to Bregma ...
• LactoSpanks: a collection of IPTG inducible promoters for the commensal lactic acid bacteria Lactobacillus gasseri

  Elsa Fristot, Guillaume Cambray, Jerome Bonnet, bioRxiv - Synthetic Biology 2023

  Quote: ... All plasmids are available from Addgene (pEF1-Phyper-spank-sfGFP ...
• Chemogenetic manipulation of endogenous proteins in fission yeast using a self-localizing ligand-induced protein translocation (SLIPT) system

  Akinobu Nakamura, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... psPAX2 (Addgene plasmid #12260, a gift from D. Trono), and pCMV-VSV-G-RSV-Rev (RIKEN BioResource Research Center plasmid RDB04393 ...
• Modular and integrative activity reporters enhance biochemical studies in the yeast ER

  Samantha G Martinusen, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... a gift from David Waugh (Addgene plasmid # 8827; http://n2t.net/addgene:8827; RRID: Addgene_8827). The desired ERS strength was encoded onto the primers used to amplify the protease genes ...
• Changes in coding and efficiency through modular modifications to a One Pot PURE system for in vitro transcription & translation

  Phuoc H. T. Ngo, et al., bioRxiv - Synthetic Biology 2023

  Quote: Plasmid pJL1-sfGFP was purchased from Addgene (cat. 69496). All the expression plasmids to construct One Pot protein factors were purchased through Addgene (cat ...
• LC3B conjugation machinery promotes autophagy-independent HIV-1 entry in CD4+ T lymphocytes

  Baptiste Pradel, et al., bioRxiv - Microbiology 2023

  Quote: ... we transfected HEK 293T cells with a combination of a donor plasmid (p-GFP: MAP1LC3B-mEGFP gifted from Allen Institute for Cell Science - Addgene Ref 101783), a purified PCR product coding for the gRNA-tracrLC3B and a plasmid coding for Streptococcus pyogenes Cas9 (pCas9 ...
• Tracing back primed resistance in cancer via sister cells

  Jun Dai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... lentiMPH v2 (Addgene, plasmid #89308) was modified by replacing Hygro selection marker with GFP to generate lentiMPH-GFP ...
• Tracing back primed resistance in cancer via sister cells

  Jun Dai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and pMD2.G (Addgene, plasmid # 12259), gifts from Didier Trono ...
• Tracing back primed resistance in cancer via sister cells

  Jun Dai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... BRCA1 was amplified from pDEST-mCherry-LacR-BRCA1 (Addgene, plasmid #71115), and BRCA2 was amplified from pCIN BRCA2 WT (Addgene ...
• Tracing back primed resistance in cancer via sister cells

  Jun Dai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... pBA439 was a gift from Jonathan Weissman (Addgene, plasmid # 85967)19 ...
• Single-deletion-mutant, third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency

  Lei Jin, et al., bioRxiv - Neuroscience 2023

  Quote: ... was made by replacing the mCre gene in pRVΔGL-4Cre (Chatterjee et al., 2018) (Addgene 98039) with the SAD B19 glycoprotein gene from pCAG-B19G (Chatterjee et al. ...
• Nanobodies against C. difficile TcdA and TcdB reveal unexpected neutralizing epitopes and provide a toolkit for toxin quantitation in vivo

  Shannon L. Kordus, et al., bioRxiv - Microbiology 2023

  Quote: ... 1/10th mass BirA (Addgene plasmid 20857), and 50 µM D-biotin at 30 °C for 1 h ...
• Alternative splicing of its 5’-UTR limits CD20 mRNA translation and enables resistance to CD20-directed immunotherapies

  Zhiwei Ang, et al., bioRxiv - Immunology 2023

  Quote: OCI-Ly8 were transduced to express green click-beetle-luciferase and ZsGreen using pHIV-Luc-ZsGreen [a gift from Bryan Welm (Addgene plasmid # 39196)] ...
• Alternative splicing of its 5’-UTR limits CD20 mRNA translation and enables resistance to CD20-directed immunotherapies

  Zhiwei Ang, et al., bioRxiv - Immunology 2023

  Quote: MXS_CMV::PuroR-bGHpA was a gift from Pierre Neveu (RRID:Addgene_62439) 44 ...
• Antiviral activity of intracellular nanobodies targeting the influenza virus RNA-polymerase core

  Mélissa Bessonne, et al., bioRxiv - Microbiology 2023

  Quote: ... 293T cells (ATCC) were co-transfected with pLV-EF1a-IRES-puro (Addgene 85132) encoding GFP or the fusion protein VHH16-2A-GFP ...
• EfpA is required for re-growth of Mycobacterium tuberculosis following isoniazid exposure

  Adam H. Roberts, et al., bioRxiv - Microbiology 2023

  Quote: ... plasmids pRH2521 and pRH2502 were gifts from Robert Husson (Addgene plasmid # 84380 and Addgene plasmid # 84379) ...
• Genome-wide CRISPRi screens reveal the essentialome and determinants for susceptibility to dalbavancin in Staphylococcus aureus

  Xue Liu, et al., bioRxiv - Microbiology 2023

  Quote: ... was amplified from pPEPZ-sgRNAclone (Addgene# 141090) with OVL2152 and OVL2153 (23) ...
• An engineered A549 cell line expressing CD13 and TMPRSS2 is permissive to clinical isolate of human coronavirus 229E

  Laurensius Kevin Lie, et al., bioRxiv - Microbiology 2023

  Quote: ... it included 6.75 μg of the packaging plasmid psPAX2 (a gift from Didier Trono; Addgene plasmid #12260), and 2.25 μg of the envelope plasmid pMD2.G (a gift from Didier Trono ...
• A dynamic in vitro model of Down Syndrome neurogenesis with Trisomy 21 gene dosage correction

  Prakhar Bansal, et al., bioRxiv - Developmental Biology 2023

  Quote: ... was cloned into AAVS1-Neo-M2rtTA 80 (Addgene plasmid #60843). The UBC promoter was PCR-amplified with flanking PvuII and FseI sites ...
• A zebrafish xenotransplant model of anaplastic thyroid cancer to study the tumor microenvironment and innate immune cell interactions in vivo

  Cassia Michael, et al., bioRxiv - Cancer Biology 2023

  Quote: Cells were transduced with lentiviral constructs encoding Luciferase and tdTomato (Addgene #72486) or the cell cycle marker PIP-FUCCI (Addgene #118616) ...
• A zebrafish xenotransplant model of anaplastic thyroid cancer to study the tumor microenvironment and innate immune cell interactions in vivo

  Cassia Michael, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or the cell cycle marker PIP-FUCCI (Addgene #118616). Transduced cells were selected by FACS sorting.
• Cholangiocytic differentiation drives cell proliferation in hepatoblastoma through Wnt-dependent FGF19 signaling

  Peng V. Wu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... EdTP (dominant negative Tcf4, Addgene #24311).
• Cholangiocytic differentiation drives cell proliferation in hepatoblastoma through Wnt-dependent FGF19 signaling

  Peng V. Wu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 0.75 μg of psPAX2 and 0.25 μg of pMD2.g (gifts from D. Trono, Addgene #12260, #12259). At 36 hrs post transfection ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 4×105 HEK 293T cells were transfected with pCDH-EF1-sCTLA-4 expression plasmid (1.5 µg) and psPax2 (2 µg) and pMD2.G (1.5 µg) packaging plasmids (Addgene), using Viafect™ (Promega ...
• Transcriptional landscape of DTP-DTEP transition reveals DUSP6 as a driver of HER2 inhibitor tolerance via Neuregulin/HER3 axis

  Majid Momeny, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and envelope vector-pMD2 (Addgene #12260) with the ratio (7:2:1 ...
• Transcriptional landscape of DTP-DTEP transition reveals DUSP6 as a driver of HER2 inhibitor tolerance via Neuregulin/HER3 axis

  Majid Momeny, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 2 μg envelope vector pMD2.G (gift from Didier Trono (Addgene plasmid # 12259) mixed in 0.45 mL water ...
• Transcriptional landscape of DTP-DTEP transition reveals DUSP6 as a driver of HER2 inhibitor tolerance via Neuregulin/HER3 axis

  Majid Momeny, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Separate lentivectors containing spCas9 (lentiCas9-Blast a gift from Feng Zhang (Addgene plasmid # 52962) and sgRNA (lentiGuide-Puro a gift from Feng Zhang (Addgene plasmid # 52963 ...
• Transcriptional landscape of DTP-DTEP transition reveals DUSP6 as a driver of HER2 inhibitor tolerance via Neuregulin/HER3 axis

  Majid Momeny, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 4 μg of packaging vector psPAX2 (gift from Didier Trono (Addgene plasmid # 12260), 2 μg envelope vector pMD2.G (gift from Didier Trono (Addgene plasmid # 12259 ...
• Transcriptional landscape of DTP-DTEP transition reveals DUSP6 as a driver of HER2 inhibitor tolerance via Neuregulin/HER3 axis

  Majid Momeny, et al., bioRxiv - Cancer Biology 2023

  Quote: ... a gift from Eric Campeau and Paul Kaufman (Addgene plasmid # 17452;http://n2t.net/addgene:17452; RRID:Addgene_17452) through Gateway cloning 61 to create pLenti-CMV-Puro-ERBB3.
• Transcriptional landscape of DTP-DTEP transition reveals DUSP6 as a driver of HER2 inhibitor tolerance via Neuregulin/HER3 axis

  Majid Momeny, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and sgRNA (lentiGuide-Puro a gift from Feng Zhang (Addgene plasmid # 52963) were produced in HEK293FT packaging cell line by transient cotransfection ...
• DENND6A couples Arl8b to a Rab34/RILP/dynein complex regulating retrograde lysosomal trafficking and autophagy

  Rahul Kumar, et al., bioRxiv - Cell Biology 2023

  Quote: ... Tmem192-3xHA (Addgene #102930), Tmem192-2xFlag (Addgene #102929 ...
• The G protein alpha Chaperone and Guanine-Nucleotide Exchange Factor RIC-8 Regulates Cilia Morphogenesis in Caenorhabditis elegans Sensory Neurons

  Christina M. Campagna, et al., bioRxiv - Cell Biology 2023

  Quote: cDNA sequences encoding N- and C-terminal fragments of Venus were amplified from pCe-BiFC-VN173 (Addgene) and pCe-BiFC-VC155 (Addgene ...
• Heat shock induces alternative polyadenylation through dynamic DNA methylation-regulated chromatin looping

  Emily E. Fink, et al., bioRxiv - Cell Biology 2023

  Quote: ... and packaging (psPAX2, AddGene) plasmids in a 2.5:1 ratio ...
• Heat shock induces alternative polyadenylation through dynamic DNA methylation-regulated chromatin looping

  Emily E. Fink, et al., bioRxiv - Cell Biology 2023

  Quote: ... with the envelope (pCMV-VSV-G, AddGene) and packaging (psPAX2 ...
• Heat shock induces alternative polyadenylation through dynamic DNA methylation-regulated chromatin looping

  Emily E. Fink, et al., bioRxiv - Cell Biology 2023

  Quote: ... and pcDNA3.1-MS2-Tet1-CD (Addgene) plasmids using Lipofectamine 2000 (Life Technologies ...
• Modeling wild type and mutant p53 in telomerase-immortalized human cells

  Jessica J. Miciak, Fred Bunz, bioRxiv - Cancer Biology 2023

  Quote: ... a gift from Feng Zhang (Addgene #62988). The resulting plasmid was introduced into RPE1 by transfection with Lipofectamine 3000 (ThermoFisher Scientific) ...
• Nuclear lamin A/C phosphorylation by loss of Androgen Receptor is a global determinant of cancer-associated fibroblast activation

  Soumitra Ghosh, et al., bioRxiv - Cancer Biology 2023

  Quote: GFP protein fused with a nuclear localization signal (GFP-NLS) was stably transduced into HDFs using lentiviruses (pTRIP-SFFV-EGFP-NLS; Addgene, Cat# 86677). Cells were cultured on eight-well coverslip-bottomed slides (ibidi GmbH ...
• Unique vulnerability of RAC1-mutant melanoma to combined inhibition of CDK9 and immune checkpoints

  Alexa C. Cannon, et al., bioRxiv - Cancer Biology 2023

  Quote: pBMN-FKBP-YFP destabilization domain plasmid was purchased from Addgene RRID:Addgene_31763 ...
• Coordinated wound responses in a regenerative animal-algal photosymbiotic metaorganism

  Dania Nanes Sarfati, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Double stranded RNA (dsRNA) was synthesized using pJC53.2 plasmids (Addgene Plasmid ID: 26536), as previously described 80 ...
• Coordinated wound responses in a regenerative animal-algal photosymbiotic metaorganism

  Dania Nanes Sarfati, et al., bioRxiv - Developmental Biology 2023

  Quote: Riboprobes for in situ hybridization were synthesized using the oligonucleotide primers listed in Supplementary Table 4 to clone the DNA fragment of interest into vector pJC53.2 (Addgene Plasmid ID: 26536), followed by riboprobe synthesis previously described 80.
• DNAJB1-PRKACA fusion protein-regulated LINC00473 promotes tumor growth and alters mitochondrial fitness in fibrolamellar carcinoma

  Rosanna K. Ma, et al., bioRxiv - Cancer Biology 2023

  Quote: ... the LeGO-lnc cloning vector served as the plasmid backbone (a gift from Dr. Jan-Henning Klusmann, Addgene plasmid #80624), which is a replication-incompetent lentiviral vector encoding a blasticidin resistance marker ...
• Anti-CD117 CAR T cells incorporating a safety switch eradicate human acute myeloid leukemia and hematopoietic stem cells

  Chiara F. Magnani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... ITRs of the pT4 vector (Addgene, 117046)23 and the iC9 gene 60 were synthesized by GeneArt (Life Technologies) ...
• Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells

  Lisa Brunet, et al., bioRxiv - Cancer Biology 2023

  Quote: The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the pHA-eIF4E plasmid (a gift from Dong-Er Zhang, Addgene plasmid # 17343) (Okumura et al. ...
• Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells

  Lisa Brunet, et al., bioRxiv - Cancer Biology 2023

  Quote: ... MS2-P65-HSF1_Hygro and sgRNA(MS2)_zeo (gifts from Feng Zhang, Addgene plasmids # 61425, # 61426 and # 61427) were used ...