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Citations for Addgene :
7851 - 7900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... starting with the HygR-P2A-AID cassete in pMGS58 (Ad-dgene #135311) (Sathyan et al. 2019) and the Puro-P2A-2xHA-FKBP_F36V casette in (Addgene #91793) (Nabet et al ...
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bioRxiv - Genomics 2023Quote: ... The Cas9-guide cassette was derived from plasmid pML104 (Addgene) (Figure S1) ...
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bioRxiv - Genomics 2023Quote: ... 4M cells were plated in a 10cm dish for 24-hours before transfecting HEK293T cells with 9ug of dCas9-mCherry-KRAB (Addgene #60954), 4ug of packing plasmids psPAX.2 and 2ug of the envelope vector pMD2.G diluted in OptiMEM medium and Trans-Ltl transfection reagent (Mirus) ...
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bioRxiv - Genomics 2023Quote: ... The second generation hSTARR-seq ORI plasmid (Addgene: #99296) was digested with Sall-HF and Agel-HF restriction enzymes ...
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bioRxiv - Genomics 2023Quote: ... 13μg of psPAX2 (gag&pol)(Addgene #12260), 7μg of pMD2G (envelope)(Addgene #12259) ...
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bioRxiv - Genomics 2023Quote: ... and 1 μg of pMD2.G (Addgene, 12259) packaging plasmids were cotransfected into 8 million HEK293T cells in a 10-cm dish supplemented with 36 μl PolyJet (SignaGen Laboratories ...
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bioRxiv - Genomics 2023Quote: The LentiGuide-Puro-P2A-EGFP (Addgene plasmid #137729) was modified to enable cloning of epegRNAs via Gibson assembly ...
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bioRxiv - Genomics 2023Quote: CHOP10.2P iPSCs were transfected with the WDR90_gRNA_Cloning vector and the pCas9_GFP vector (which was a gift from Kiran Musunuru, Addgene plasmid #44719 ...
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bioRxiv - Genomics 2023Quote: ... tetO-ASCL1-LMX1B-NURR1-PuroR (Addgene #182298), tetO-ASCL1-PuroR (Addgene #97329) ...
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bioRxiv - Genomics 2023Quote: ... The lentiV2-pegRNA and lentiV2-ngRNA plasmids were constructed by replacing the Cas9 and Puromycin sequences in the lentiCRISPR v2 plasmid (Addgene, 52961), with hygromycin B and EGFP sequences ...
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bioRxiv - Genomics 2023Quote: ... with 3 μg of psPAX (Addgene, 12260) and 1 μg of pMD2.G (Addgene ...
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bioRxiv - Genomics 2023Quote: CHOP10.2P iPSCs were transfected with the WDR90_gRNA_Cloning vector and the pCas9_GFP vector (which was a gift from Kiran Musunuru, Addgene plasmid #44719, http://n2t.net/addgene:44719, RRID: Addgene_44719)21 using a lipid-mediated process (Lipofectamine STEM ...
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bioRxiv - Genomics 2023Quote: ... The WDR90_gRNA was ligated into a AflII digested gRNA_Cloning Vector (which was a gift from George Church, Addgene plasmid #41824 ...
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bioRxiv - Genomics 2023Quote: ... were transformed with pET-28b-Cas9-His (Addgene, no. 47327) by heat shock following the manufacturers’ instructions ...
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bioRxiv - Genomics 2023Quote: ... The WDR90_gRNA was ligated into a AflII digested gRNA_Cloning Vector (which was a gift from George Church, Addgene plasmid #41824, http://n2t.net/addgene:41824, RRID: Addgene_41824)20 using the NEB Gibson Assembly Kit to generate a WDR90_gRNA_Cloning vector.
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bioRxiv - Immunology 2023Quote: ... 0.25µg envelope vector pMD2.G (RRID:Addgene_12259), 2µg of packaging plasmid pCMV-dR8.91 (Creative Biogene) ...
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bioRxiv - Genomics 2023Quote: ... The amplified libraries were cloned into the CROPseq vector (Addgene #86708) via Golden Gate assembly using BsmBI restriction sites as previously described15 ...
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bioRxiv - Genomics 2023Quote: ... Cas9 activity was evaluated using the fluorescence- based reporter pXPR011 (Addgene #59702), which expressed GFP and cognate sgRNA to assess GFP knockdown upon successful CRISPR activity ...
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bioRxiv - Genomics 2023Quote: The A549-TetR-Cas9 cell line was created by simultaneously transfecting A549 cells with piggyBac transposase and a piggyBac cargo plasmid containing TetR-inducible Cas9 (Addgene #134247), and selecting for 7 days with 500 ug/mL G418 ...
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bioRxiv - Genomics 2023Quote: ... the entry vector was constructed by adding a bcl-2 splice acceptor and four SV40 polyA-signals to a plasmid backbone based on pSMART (Addgene #49157) containing a green fluorescent protein (GFP ...
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bioRxiv - Genomics 2023Quote: The pU6-pegRNA-GG-acceptor (Addgene plasmid #132777) was digested with BsaI-HFv2 (New England Biolabs ...
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bioRxiv - Genetics 2023Quote: ... or the SpG variant capable of targeting sites encoding NGA PAMs (RTW4177, Addgene plasmid # 139988) (Supplementary table 2) ...
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bioRxiv - Genetics 2023Quote: ... following the manufacturer’s protocol with AAVS1 targeting vector and predesigned transcription activator-like effector nucleases (hAAVS1 TALEN Left and Right were gifts from Su-Chun Zhang, Addgene plasmid # 52341 & 52342 ...
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bioRxiv - Genetics 2023Quote: ... in an all-in-one tetracycline- inducible expression cassette with AAVS1 homology arms (AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343 ...
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bioRxiv - Genetics 2023Quote: ... which was a gift from Ken-Ichi Takemaru (Addgene plasmid # 118055 ...
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bioRxiv - Genetics 2023Quote: ... they were cloned using the Gateway LR reaction into the pCFJ150 targeting vector (a gift from Erik Jorgensen; Addgene plasmid # 19329) (Frøkjær-Jensen et al ...
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bioRxiv - Genomics 2023Quote: ... psPAX2 (RRID: Addgene_12260) and pMD2.G (RRID ...
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bioRxiv - Genomics 2023Quote: ... and pMD2.G (RRID: Addgene_12259) and its titration was determined as previously described [22] ...
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bioRxiv - Genomics 2023Quote: ... into the SbfI/AgeI site of the pLS-SceI vector (Addgene, cat ...
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bioRxiv - Genetics 2023Quote: ... or Lenti-guide blast (a gift from Brett Stringer (Addgene plasmid #104993; http://n2t.net/addgene:104993; RRID:Addgene_104993)) plasmids (102 ...
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bioRxiv - Genetics 2023Quote: ... were cloned into a Lenti-guide puro (a gift from Feng Zhang (Addgene plasmids #52963 ...
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bioRxiv - Genetics 2023Quote: ... or Lenti-guide blast (a gift from Brett Stringer (Addgene plasmid #104993 ...
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bioRxiv - Genetics 2023Quote: ... We cloned oligonucleotides encoding the gRNA spacers into BPK1520 (Addgene plasmid # 65777) to generate gRNA expression plasmids ...
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bioRxiv - Genetics 2023Quote: ... pMD2.G and psPAX2 were a gift from Didier Trono (Addgene plasmids #12259 and 12260 ...
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bioRxiv - Genomics 2023Quote: ... We replaced the hU6-sgRNA cassette with the mU6-sgRNA cassette from pMK1334 (ref. 1) (Addgene plasmid #127965, RRID:Addgene_127965) by restriction cloning between sites MluI and XbaI ...
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bioRxiv - Genomics 2023Quote: ... was generated using the pAAV-U6-sgRNA-CMV-GFP plasmid as the starting backbone (a gift from Hetian Lei, Addgene plasmid # 85451, RRID:Addgene_85451)20 ...
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bioRxiv - Genomics 2023Quote: ... The Lox66 and Lox71 sequences and their orientation was copied from the pFrt-invCAG-Luc (a gift from Ivo Huijbers, Addgene plasmid #63577, RRID:Addgene_63577)23 and were inserted along with the 175-bp intervening spacer as a gBlock at the XbaI-KpnI multiple cloning site.
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bioRxiv - Genomics 2023Quote: To generate a functional mouse sgRNA pooled library of the pAP210 and pAP215 plasmids, we transferred the sgRNA sequences from the mCRISPRi-v2 M1 (Kinases, Phosphatases, and Drug Targets) gRNA pooled library (Addgene pooled library #83989)10 ...
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bioRxiv - Genomics 2023Quote: ... 20 μg of pAdDeltaF6 (a gift from James M. Wilson, Addgene plasmid # 112867, RRID:Addgene_112867), 7 μg of pAP215-M1 library plasmid ...
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bioRxiv - Genomics 2023Quote: ... The Lox66 and Lox71 sequences and their orientation was copied from the pFrt-invCAG-Luc (a gift from Ivo Huijbers, Addgene plasmid #63577, RRID:Addgene_63577)23 and were inserted along with the 175-bp intervening spacer as a gBlock at the XbaI-KpnI multiple cloning site.
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bioRxiv - Genetics 2023Quote: ... We used gDNA from Cas9 flies to amplify the homology arms which we cloned to pScarlessHD-3xFLAG-DsRed plasmid (gift from Kate O’Connor-Giles, Addgene plasmid # 80820) with Gibson Assembly (NEB).
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bioRxiv - Genetics 2023Quote: ... GFP-3’UTR was PCR amplified using primers (5’-AGCTTGCATGCCTGCAGGTCG-3’ and 5’-AAGGGCCCGTACGGCCGACTA-3’) and plasmid pPD95.75 (Plasmid #1494-Addgene) as the template ...
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bioRxiv - Genetics 2023Quote: ... All the plasmids developed in this work (Table 2) will be available from Addgene (Massachusetts, USA) once the manuscript is accepted for publication.
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bioRxiv - Genetics 2023Quote: CRIMPkit vectors are available from Addgene as a plasmid collection ...
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bioRxiv - Cell Biology 2023Quote: ... 49 (Addgene, 20668) using electroporation ...
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bioRxiv - Cell Biology 2023Quote: ... and the insert encoding AcGFP that was isolated from pAc-GFP-Sec61beta (from Tom Rapoport, Addgene #15108) restricted with BglII ...
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bioRxiv - Cell Biology 2023Quote: ... pSFG-TFII-I-GFP delta and pSFG-TFII-I-GFP Y248&249F were obtained from Addgene(#22199, #22190, #22196).
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bioRxiv - Evolutionary Biology 2023Quote: ... and subcloned into both pHemmarG (Addgene #31221) for CD4:tdGFP reporters ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into pLKO-Tet-On (Addgene #21915).
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... (pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138).