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7501 - 7550 of 10000+ citations since 2019

• FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe

  Lucy Jennings, et al., bioRxiv - Cell Biology 2023

  Quote: ... hCas9 (Addgene# 41815) and pcDNA 3.1 Hygromycin (Invitrogen ...
• Crosstalk between the plasma membrane and cell-cell adhesion maintains epithelial identity for correct polarised cell divisions

  Manal M. Hosawi, et al., bioRxiv - Cell Biology 2023

  Quote: ... 5 μg packaging plasmid pUMVC (Addgene, Plasmid #8449), 6.5 μg envelope plasmid pCMV-VSV-G (Addgene ...
• SLC12A9 is a lysosome-detoxifying ammonium – chloride co-transporter

  Roni Levin-Konigsberg, et al., bioRxiv - Cell Biology 2023

  Quote: SLC12A9-GFP was cloned by amplifying the ORF from pDONR221_SLC12A9 (Addgene plasmid #131897) and inserting it into a backbone containing GFP and a Blasticidin resistance cassette ...
• Peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involved

  Tamara Somborac, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells were co-transfected with two gRNAs and pSpCas9n(BB)-2A-Puro (PX462) V2.0 (Addgene, 62987). After 24 h the cells were selected with puromycin and expanded ...
• Peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involved

  Tamara Somborac, et al., bioRxiv - Cell Biology 2023

  Quote: ... pLL3.7 (Addgene, 11795) was cut with EcoRI (NEB ...
• Peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involved

  Tamara Somborac, et al., bioRxiv - Cell Biology 2023

  Quote: ... pMD2.G (Addgene, 12259), psPAX2 (Addgene,12260 ...
• Peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involved

  Tamara Somborac, et al., bioRxiv - Cell Biology 2023

  Quote: ... psPAX2 (Addgene,12260) and pLL3.7-EGFP-YgiM(TA ...
• Real Time In vivo Analysis of Pancreatic Beta-cell Autophagic Flux Reveals Impairment Before Onset of Autoimmune Diabetes

  Olha Melnyk, et al., bioRxiv - Cell Biology 2023

  Quote: ... we generated AAV8-RIP1-mCherry-EGFP-LC3B plasmid by cloning the insulin/beta globin promoter and the mCherry-EGFP-LC3B construct (from Addgene construct #22418, (28)) into an AAV8 packaging plasmid ...
• PRMT1 Inhibition Selectively Targets BNC1-Dependent Proliferation, but not Migration in Squamous Cell Carcinoma

  Rafik Boudra, et al., bioRxiv - Cancer Biology 2023

  Quote: ... pLenti-CMV-GFP-Blast (659-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17445). pRL-SV40P was a gift from Ron Prywes (Addgene plasmid # 27163) ...
• Primate TRIM34 is a broadly-acting, TRIM5-dependent lentiviral restriction factor

  Joy Twentyman, et al., bioRxiv - Immunology 2023

  Quote: ... pLentiCRISPR-v2 containing guides were transfected wiTHPSPAX2 (Addgene #12260, a gift from Didier Trono) and pMD2.G to generate lentiviral particles ...
• Primate TRIM34 is a broadly-acting, TRIM5-dependent lentiviral restriction factor

  Joy Twentyman, et al., bioRxiv - Immunology 2023

  Quote: ... [47] or pHIV-zsGreen (Addgene #18121 ...
• Functional genomics identifies extension of complex N-glycans as a mechanism to evade lysis by natural killer cells

  Xiaoxuan Zhuang, et al., bioRxiv - Immunology 2023

  Quote: The sgRNAs that targeted specific genes were synthesized by Integrated DNA Technologies (IDT) and cloned into the BsmBI restriction sites of the LentiGuide-Puro vector (Addgene #52963) using the In-Fusion HD cloning kit (Clontech) ...
• Paradoxical increases in anterior cingulate cortex activity during nitrous oxide-induced analgesia reveal a signature of pain affect

  Jarret AP Weinrich, et al., bioRxiv - Neuroscience 2023

  Quote: ... we delivered GCaMP6f under control of the synapsin promoter (AAV1/9-SYN-GCaMP6f; Addgene, #100837)51 ...
• Dorsal peduncular cortex activity modulates affective behaviors in mice

  Justin J. Botterill, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV5-hSyn-hM4D(Gi)-mCherry (8.6 x 1012 vg/mL, Addgene #50475), and AAV5-hSyn-hM3D(Gq)-mCherry (1.9 x 1013 vg/mL ...
• Dorsal peduncular cortex activity modulates affective behaviors in mice

  Justin J. Botterill, et al., bioRxiv - Neuroscience 2023

  Quote: ... We used the following adeno-associated viruses (AAVs): AAV5-hSyn-mCherry (2.3 x 1013 vg/mL, Addgene #114472), AAV5-hSyn-hM4D(Gi)-mCherry (8.6 x 1012 vg/mL ...
• Prefrontal projections modulate recurrent circuitry in insular cortex to support short-term memory

  Jian Yao, et al., bioRxiv - Neuroscience 2023

  Quote: The vectors of optogenetics experiments were obtained from AddGene. Packages of AAV8 viruses were provided by Obio Technology Co ...
• ZBTB7A regulates MDD-specific chromatin signatures and astrocyte-mediated stress vulnerability in orbitofrontal cortex

  Sasha L. Fulton, et al., bioRxiv - Neuroscience 2023

  Quote: ... or AAV5-gfaABC1D-cyto-GCaMP6fto (Addgene # 52925-AAV5) to ensure selective expression solely in neurons or in astrocytes ...
• ZBTB7A regulates MDD-specific chromatin signatures and astrocyte-mediated stress vulnerability in orbitofrontal cortex

  Sasha L. Fulton, et al., bioRxiv - Neuroscience 2023

  Quote: ... Primary mixed cultures were transduced with AAV1-hSyn-GCaMP6f (Addgene # 100837-AAV1) or AAV5-gfaABC1D-cyto-GCaMP6fto (Addgene # 52925-AAV5 ...
• ZBTB7A regulates MDD-specific chromatin signatures and astrocyte-mediated stress vulnerability in orbitofrontal cortex

  Sasha L. Fulton, et al., bioRxiv - Neuroscience 2023

  Quote: ... on a cohort of 8 week old C57BL/6J male mice that were injected with the pAAV-hSyn-hM4D(Gi)-mCherry to express the inhibitory Gi DREADD (Addgene #50475-AAV2), in combination with either the rAAV6-GFAP-Zbtb7a OE construct or the rAAV6-GFAP-GFP empty control vector into the OFC 3 weeks previously ...
• ASTRA: a deep learning algorithm for fast semantic segmentation of large-scale astrocytic networks

  Jacopo Bonato, et al., bioRxiv - Neuroscience 2023

  Quote: ... 0.4 μl of AAV solution containing pZac2.1 gfaABC1D-cyto-GCaMP6f (Addgene viral prep # 52925-AAV5) was injected at 50 nL/min using a hydraulic injection apparatus driven by a syringe pump (UltraMicroPump ...
• Fluorescent Sensors for Imaging Interstitial Calcium

  Ariel A Valiente-Gabioud, et al., bioRxiv - Physiology 2023

  Quote: Mammalian and bacterial expression vectors coding for GreenT-ECs will be made available from Addgene. The crystal structure of variant GreenT NRS F241Y will be deposited at https://www.rcsb.org/
• Split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80

  Ben Ewen-Campen, et al., bioRxiv - Genetics 2023

  Quote: ... we used Gibson Assembly to replace the Gal4DBD sequence in pBPZpGAL4DBDUw (Addgene 26233). The 2.3kb enhancer fragment VT024642 was amplified from genomic DNA using these primers ...
• Proximity mapping of desmosomes reveals a striking shift in their molecular neighbourhood associated with maturation

  Judith Barbara Fuelle, et al., bioRxiv - Cell Biology 2023

  Quote: ... Plasmid DNA containing desmosome genes were purchased from Addgene, [Dsc2a no ...
• Proximity mapping of desmosomes reveals a striking shift in their molecular neighbourhood associated with maturation

  Judith Barbara Fuelle, et al., bioRxiv - Cell Biology 2023

  Quote: ... BioID vectors pCDH-BirA-myc and pCDNA3.1-myc-BioID (Addgene no. 35700 (Roux et al., 2012)) were gifts from A ...
• Leukemic stem cells hijack lineage inappropriate signalling pathways to promote their growth

  Sophie G Kellaway, et al., bioRxiv - Cancer Biology 2023

  Quote: ... the fragment was ligated into tRMPVIR (Addgene) plasmid ...
• Group comparison based on genetic information reveals lineage-specific therapeutic vulnerabilities in acute myeloid leukemia

  Jakushin Nakahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... were gifts from Benjamin Ebert.27 lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963 ...
• Group comparison based on genetic information reveals lineage-specific therapeutic vulnerabilities in acute myeloid leukemia

  Jakushin Nakahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and pLKO5.sgRNA.EFS.GFP (Addgene plasmid # 57822 ; http://n2t.net/addgene:57822 ; RRID:Addgene_57822) were gifts from Benjamin Ebert.27 lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963 ...
• Group comparison based on genetic information reveals lineage-specific therapeutic vulnerabilities in acute myeloid leukemia

  Jakushin Nakahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and pLKO5.sgRNA.EFS.GFP (Addgene plasmid # 57822 ...
• Group comparison based on genetic information reveals lineage-specific therapeutic vulnerabilities in acute myeloid leukemia

  Jakushin Nakahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... pLKO5.sgRNA.EFS.tRFP657 (Addgene plasmid # 57824 ; http://n2t.net/addgene:57824 ; RRID:Addgene_57824) and pLKO5.sgRNA.EFS.GFP (Addgene plasmid # 57822 ...
• Group comparison based on genetic information reveals lineage-specific therapeutic vulnerabilities in acute myeloid leukemia

  Jakushin Nakahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... were gifts from Benjamin Ebert.27 lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963 ; http://n2t.net/addgene:52963 ; RRID:Addgene_52963).28 The Cas9 expression in THP1 ...
• Group comparison based on genetic information reveals lineage-specific therapeutic vulnerabilities in acute myeloid leukemia

  Jakushin Nakahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... RRID:Addgene_52962).28 FUCas9Cherry was a gift from Marco Herold (Addgene plasmid # 70182 ; http://n2t.net/addgene:70182 ; RRID:Addgene_70182).29 Lentiviruses were produced by transient transfection of 293T cells with viral plasmids along with gag- ...
• Group comparison based on genetic information reveals lineage-specific therapeutic vulnerabilities in acute myeloid leukemia

  Jakushin Nakahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and psPAX2 (Addgene plasmid #12260; http://n2t.net/addgene:12260; RRID: Addgene_12260) were gifts from Didier Trono ...
• Group comparison based on genetic information reveals lineage-specific therapeutic vulnerabilities in acute myeloid leukemia

  Jakushin Nakahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid # 52962 ; http://n2t.net/addgene:52962 ; RRID:Addgene_52962).28 FUCas9Cherry was a gift from Marco Herold (Addgene plasmid # 70182 ...
• ZEB1-dependent modulation of fibroblast polarization governs inflammation and immune checkpoint blockade sensitivity in colorectal cancer

  Harald Schuhwerk, et al., bioRxiv - Cancer Biology 2023

  Quote: The hCas9 and gRNA_GFP-T2 plasmids were a kind gift from George Church (Mali, et al. 2013, Addgene plasmids #30205 and #41820) and the Apc sgRNA plasmid kindly provided by Hans Clevers 61 ...
• Modulation of SLFN11 induces changes in DNA Damage response

  Christophe Michel Raynaud, et al., bioRxiv - Cancer Biology 2023

  Quote: PB-UniSAM containing mCherry was a gift from Lesley Forrester (Addgene plasmid # 99866; http://n2t.net/addgene:99866; RRID: Addgene_99866) (Fidanza et al ...
• Arf1-PI4KIIIβ positive vesicles regulate phosphatidylinositol-3-phosphate signalling to facilitate the fission of lysosomal tubules

  Maxime Boutry, et al., bioRxiv - Cell Biology 2023

  Quote: ... and mCherry-FKBP-MTM1 was a gift from Tamas Balla (Addgene #51614); the bacterial expression vector pBAD::mRFP1 was a gift from Robert Campbell (Addgene #54667) ...
• Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation

  Norbert Minet, et al., bioRxiv - Cell Biology 2023

  Quote: ... or on mCherry expression for the pLVX vector (RRID:Addgene_174088) using cell sorter (Sony SH800) ...
• RhoB promotes Salmonella survival by regulating autophagy

  Marco Kirchenwitz, et al., bioRxiv - Cell Biology 2023

  Quote: ... pSpCas9(BB)-2A-GFP (PX458) was from Feng Zhang (Addgene plasmid # 48138 ...
• Allele pairing at Sun1-enriched domains at the nuclear periphery via T1A3 tandem DNA repeats

  Gayan I. Balasooriya, David L. Spector, bioRxiv - Cell Biology 2023

  Quote: ... First pCR4-24xMS2SL (Addgene, Cat.No.31865) and pCR-24xPP7SL (Addgene ...
• DDX5 (p68) orchestrates β-catenin, RelA and SP1 mediated MGMT gene expression: Implication in TMZ chemoresistance

  Rajni Shaw, et al., bioRxiv - Cancer Biology 2023

  Quote: shRNA against β-catenin was purchased from Addgene (pLKO.1 puro shβ-catenin; Addgene cat no.18803). shRNA targeting RelA and SP1 were cloned into pLKO.1-Puro vector individually according to Addgene’s protocol ...
• Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ

  Zhi Yang Tan, et al., bioRxiv - Cell Biology 2023

  Quote: ... and pFA6a-link-yoTagRFP-T-Kan (Lee et al., 2013) was a gift from Wendell Lim & Kurt Thorn (Addgene 44906); both were given in the form of bacterial stabs ...
• Aversive experience drives offline ensemble reactivation to link memories across days

  Yosif Zaki, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV1-Syn-GCaMP6f-WPRE-SV40 (titer: 2.8 × 10^13 GC/mL) was purchased from AddGene and was diluted 1:4 in sterile 1× PBS ...
• CDK activity at the centrosome regulates the cell cycle

  Emma L. Roberts, et al., bioRxiv - Cell Biology 2023

  Quote: ... pFA6a-KanMX-tetO-pCyc1 (TetP) (Addgene plasmid # 41023 ...
• CDK activity at the centrosome regulates the cell cycle

  Emma L. Roberts, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following plasmids were both gifts from Michael Nick Boddy and bought from Addgene: pFA6a-KanMX-tetO-pCyc1 (TetP ...
• Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq

  Sara Sunshine, et al., bioRxiv - Systems Biology 2023

  Quote: ... The BsmBI-flanked spacer was then replaced by a fragment amplified from pJR98 (Addgene #140096), carrying the constant region of the first sgRNA and the promoter for the second one ...
• Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq

  Sara Sunshine, et al., bioRxiv - Systems Biology 2023

  Quote: ... We used a lentiviral backbone (pJR101, a variant of pJR85, Addgene #140095 ...
• Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq

  Sara Sunshine, et al., bioRxiv - Systems Biology 2023

  Quote: The CRISPRi Calu-3 cell line was generated by lentiviral delivery of pMH0001 (UCOE-SFFV-dCas9-BFP-KRAB) (19) (Addgene #85969) into Calu-3 cells ...
• Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior

  Dakota F. Brockway, et al., bioRxiv - Neuroscience 2023

  Quote: ... was packaged by Vector Biolabs using a plasmid from Addgene (#118273). AAV encoding Cre-dependent TdTomato (AAV9.CAG.FLEX.TdTomato ...
• Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior

  Dakota F. Brockway, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV encoding Cre-dependent TdTomato (AAV9.CAG.FLEX.TdTomato) was purchased from Addgene (#51503). Titers of the GCaMP6f and TdTomato viruses were 1×1012 and 1.9×1013 GC/mL ...
• Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior

  Dakota F. Brockway, et al., bioRxiv - Neuroscience 2023

  Quote: The viral construct AAV5-EF1a-DIO-hChR2(H134R)-eYFP-WPRE-HGHpA (Titer=2.1×1013 GC/mL) was obtained from Addgene (#20298). Male and female SST-IRES-Cre+/- mice at least 8 weeks old were deeply anesthetized with 5% isoflurane and underwent stereotaxic surgery ...