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Citations for Addgene :
7601 - 7650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Virus (AAV1-S5E2-jGCaMP6f; Addgene #135632-AAV1; diluted 1:3 in saline) was injected into the RSC (AP ...
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bioRxiv - Neuroscience 2023Quote: ... all mice received injections of a hM3Dq virus (pAAV-S5E2-Gq-P2A-dTomato; Addgene #135635-AAV1)58.
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bioRxiv - Neuroscience 2023Quote: ... AAV1-CamKII-GCaMP6f (Addgene, #100834) was injected into CA1 (∼75 nl ...
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bioRxiv - Cell Biology 2023Quote: ... the H2B-mRFP1 sequence was amplified by PCR from pLV-RFP (Addgene #26001 (39)) and inserted using Gibson assembly in place of GFP into vector pEGFP-C1 (Clonetech ...
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bioRxiv - Cell Biology 2023Quote: ... but using pLV-dimericTomato-2x-rGBD (a gift from Dorus Gadella; Addgene #176098) as transfer plasmid in HEK293T cells ...
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bioRxiv - Neuroscience 2023Quote: ... and pMD2.G (Addgene #12259) (mixed 1:1:1 ...
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bioRxiv - Neuroscience 2023Quote: ... pMDLg/pRRE (Addgene #12251), and pMD2.G (Addgene #12259 ...
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bioRxiv - Neuroscience 2023Quote: ... the custom library transfection mix was prepared in the following manner: 15 µg of custom library plasmid and 15 µg of third-generation packaging DNA mix containing pRSV-REV (Addgene #12253), pMDLg/pRRE (Addgene #12251) ...
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bioRxiv - Neuroscience 2023Quote: ... Two small holes were made in the skull above the injection sites and 200nl (100nl/min) of AAV8-hSyn-DIO-mCherry or AAV8- hSyn-DIO-hm4Di-mCherry virus (Addgene, Watertown, MA) was infused in both hemispheres of the PVN using a 1µL Hamilton syringe (0° angle ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV-hSyn-hM4D(Gi)-mCherry was a gift from Bryan Roth (Addgene, 50475; http://n2t.net/addgene). The medium was changed on D7 and neurons were seeded with 1.5 µg/mL K18-tau fibrils ...
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bioRxiv - Neuroscience 2023Quote: ... pGP-AAV-hSyn-jGCaMP8f-WPRE was a gift from GENIE Project (Addgene, 162376). The medium was changed on D7 ...
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bioRxiv - Neuroscience 2023Quote: ... UBA5) were generated as described above with the following modifications: third-generation packaging DNA mix containing pRSV-REV (Addgene #12253), pMDLg/pRRE (Addgene #12251) ...
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bioRxiv - Neuroscience 2023Quote: ... and pMD2.G (Addgene #12259) (molarity 1:1 ...
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bioRxiv - Neuroscience 2023Quote: ... pMDLg/pRRE (Addgene #12251), and pMD2.G (Addgene #12259 ...
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Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complexbioRxiv - Cell Biology 2023Quote: ... LifeAct7-mCherry was a gift from Michael Davidson (Addgene plasmid #54491) (Riedl et al. ...
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bioRxiv - Cell Biology 2023Quote: ... and pMD2.G (#12253, #12251, #12259; Addgene) as previously described59 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were co-transfected with pMDL (Addgene #12251), pREV (Addgene #12253) ...
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bioRxiv - Cell Biology 2023Quote: The sequence coding for Halo tag was amplified from pSEMS-Halo7Tag-hFis (111136 Addgene) vector by using primers (5’-GCAATTCGATATGGGATCCGAAATCGGTACTGGCTTT CC-3’ and 5’-GGCCTCGAGATTAACCGGAAATCTCCAGAGTAG-3’).
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bioRxiv - Cell Biology 2023Quote: ... Obtained pcDNA-Halo was further digested with HindIII/Bam-HI restriction enzymes and ligated with the NPM insert (obtained by PCR amplification from eGFP-NPM (17578 Addgene) with our primers 5’-CCCAAGCTTCCACCATGGAAGATTCGATGGACATGG-3’ and 5’-CGGGATCCAAGAGACTTCCTCCACTGCC-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNAs were introduced into pLenti-sgRNA backbone (Addgene #71409) with BsmBI digestion (New Englands Biolabs #R0739 ...
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bioRxiv - Neuroscience 2023Quote: ... and pMD2.G (Addgene #12259) (mixed 1:1:1) ...
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bioRxiv - Neuroscience 2023Quote: ... transfection mix for each plasmid was prepared in the following manner: 1 µg of transfer plasmid and 2 µg of second-generation packaging DNA mix containing psPAX (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Neuroscience 2023Quote: ... This lentiviral construct was made using the 3rd generation lentiviral plasmid FUGW (Addgene, 14883), where the PacI + EcoRI fragment was replaced by the hSyn-GCaMP8f fragment (Zhang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... This lentiviral construct was made using the 3rd generation lentiviral plasmid FUGW (Addgene, 14883), where the PacI + EcoRI fragment was replaced by hSyn-hM4D(Gi)-mCherry ...
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bioRxiv - Cell Biology 2023Quote: ... or S170A-ABHD17B-FLAG along with pMD2.G (Addgene plasmid # 12259) and psPAX2 (Addgene plasmid # 12260 ...
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bioRxiv - Cell Biology 2023Quote: ... and psPAX2 (Addgene plasmid # 12260) using X-tremeGENE 9 transfection reagent[22] ...
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bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were co-transfected with vectors the ORF3a proteins and the vector or 4xmts-Neon-Green (mitochondria; Addgene, #98876). At 48 h post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and pMRX-IP-HaloTag7-LC3 (Addgene #184899) were used for retroviral generation of ATG3 KO/HaloTag7-LC3 stable cell line ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids of pVSV-G (Addgene #138479), pBS-CMV-gagpol (Addgene #35614 ...
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bioRxiv - Cell Biology 2023Quote: ... that contain CRISPR sequences were annealed and ligated into BbsI-linearized pSpCas9(BB)-2A-GFP vector77 (a gift from Feng Zhang; Addgene plasmid # 48138). Sequence-verified gRNA constructs were then transfected into HeLa cells for 24 h and GFP-positive cells were individually sorted by fluorescence activated cell sorting (FACS ...
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bioRxiv - Cell Biology 2023Quote: ... pBS-CMV-gagpol (Addgene #35614) and pMRX-IP-HaloTag7-LC3 (Addgene #184899 ...
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bioRxiv - Cell Biology 2023Quote: ... pUCmini-iCAP-PHP.S was a gift from Viviana Gradinaru (Addgene plasmid #103006; http://n2t.net/addgene:103006; RRID:Addgene_103006). The helper plasmid used was pHelper (TaKaRa).
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bioRxiv - Cell Biology 2023Quote: ... cDNAs from Addgene plasmids #111906 and #111905 were PCR amplified ...
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bioRxiv - Cell Biology 2023Quote: ... and DNA was purified before ligation into pJR103 (Addgene plasmid #187242). The ligated DNA was precipitated using isopropanol (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting fragments and mCherry cDNA amplified from Addgene plasmid #102245 were assembled using Gibson Assembly ...
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bioRxiv - Cell Biology 2023Quote: ... Lenti-Cas9-blast (a kind gift of F. Zhang; Addgene #52962) or TRIPZ (Open Biosystems/Horizon ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GFP-P4M-SidMx2 (Addgene plasmid #51472), fyve mApple GRK2ct (created by fusing 2XFYVE domains (75) ...
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bioRxiv - Cell Biology 2023Quote: ... pFA6a-GBP-mCherry-kanMX6 was a gift from Quanwen Jin (Addgene plasmid # 89068 ...
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bioRxiv - Cell Biology 2023Quote: ... The donor plasmid was cloned into the empty backbone pUC19 cloning vector (Addgene #50005) digested with SaII and KpnI – the 5’ and 3’ homology arms at the TTC1 C-terminus appended with the TTC1 protospacer + PAM sequence on both ends ...
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bioRxiv - Cell Biology 2023Quote: Expression constructs used in this study include pCI-mScarlet (Addgene #85042; pC1-mScarlet-Syp [7]), pCIG2-GFP-MACF43 [42] ...
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bioRxiv - Neuroscience 2023Quote: ... to PV-Cre mice and pENN-AAV1-hSyn-Cre-WPRE-hGH (Addgene: 105553) to Ai6 mice ...
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bioRxiv - Neuroscience 2023Quote: ... td-tomato-C1 (SNAP*tdTom clone) was replaced by mCherry (Plasmid #238287 Addgene) using XhoI ...
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bioRxiv - Neuroscience 2023Quote: ... The CFP-GCaMP7s-tdTom were inserted into pAAV-CAG-eYFP (Plasmide #104055 Addgene), by replacing the YFP with the restriction enzymes XbaI (5’ ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128) or AAV9-Ef1a-DIO-mCherry (UNC Vector core ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128), and AAV2/1-hSyn-DIO-GreenDownwardcADDis (Boston Children’s Hospital Vector Core ...
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bioRxiv - Neuroscience 2023Quote: We subcloned split-sfGCaMP6s2 (from pGP-GCaMP6s-27) to another AAV compatible plasmid (Addgene, Plasmid#162374). We removed the M13-sfGFP1-6 fragment by digesting it from clone TOM-M13-cpGFP (Suppl ...
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bioRxiv - Neuroscience 2023Quote: ... We used sfGFP-N1 (Plasmid #54737 Addgene) as the backbone for inserting the stop codon and deleted the following FNSH residues via reverse PCR ...
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bioRxiv - Neuroscience 2023Quote: ... the plasmid 119739 (Addgene) was used as a template ...
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bioRxiv - Neuroscience 2023Quote: We used AAV-9-syn-GCaMP6s vector with synapsin promoter (pGP-AAV-syn-GCaMP6s-WPRE.4.641 at a titre of 1x1013 GC·ml-1, Addgene, Watertown, MA, USA), and therefore it transduced neurons showing higher tropism for the AAV 2/9 subtype ...
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bioRxiv - Biophysics 2023Quote: Dendra-2 EEA1 was generated by replacing TagRFP-T in RagRFP-T EEA1 (Addgene plasmid #42635) at cloning sites AgeI and XhoI ...