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Citations for Addgene :
7551 - 7600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and psPAX2 (Addgene #12260) by Lipofectamine 3000 (Life technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the gene editing crispr-cas9 system (lentiCRISPR-v2) (Addgene #52961). sgRNAs were designed using the CRISPR tool (http://crispr.mit.edu ...
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bioRxiv - Cancer Biology 2023Quote: ... a gift of Dmitri Wiederschain (Addgene plasmid #21915). 293T cells were transfected with shRNA constructs and packaging plasmids (psPAX2 and VSV-G ...
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bioRxiv - Cancer Biology 2023Quote: ... as described previously.16,49 Sting knockdown in the MSI MC38 CRC cells was generated as described previously using shRNA and the pLKO.1 system.16,50 Ovalbumin (OVA) expressing MSI and CIN MC38 CRC cells were made by transfection with the pCI-neo-cOVA plasmid (Addgene #25097) and selection with 200 µg/ml G418.51
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bioRxiv - Cancer Biology 2023Quote: The lentiviral construct for the constitutive expression of the bioluminescence (firefly luciferase) and the red fluorescence (mCherry) was generated by amplifying the cDNA encoding both genes from Addgene Plasmid #44965 and cloned in the lentivirus backbone (Addgene #12262 ...
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bioRxiv - Cancer Biology 2023Quote: ... lentiCRISPRv2 puro was a gift from Brett Stringer (Addgene plasmid # 98290). Single-guide RNAs were designed through MIT CRISPR Designer (crispr.mit.edu) ...
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bioRxiv - Cancer Biology 2023Quote: ... and lentiCRISPRv2 hygro which was a gift from Brett Stringer (Addgene plasmid # 98291).
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bioRxiv - Cancer Biology 2023Quote: ... cloned into pLenti PGK Neo DEST (pLenti PGK Neo DEST (w531-1) a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19067) by using the Gateway LR Clonase II Enzyme Mix (Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: pDONR223_MDM2_WT was a gift from Jesse Boehm & Matthew Meyerson & David Root (Addgene plasmid # 82897) cloned into pLenti PGK Neo DEST (pLenti PGK Neo DEST (w531-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... ILK1 and TAZ-S89A (Addgene plasmids #32840) were amplified by PCR and cloned into pLenti-GFP-puro (Addgene plasmids #17481 ...
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bioRxiv - Cancer Biology 2023Quote: ... pDEST/Lifeact-mCherry-N1 was a gift from Robin Shaw (Addgene plasmid # 40908), pmCherry Paxillin was a gift from Kenneth Yamada (Addgene plasmid # 50526 ...
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bioRxiv - Cancer Biology 2023Quote: ... pmCherry Paxillin was a gift from Kenneth Yamada (Addgene plasmid # 50526) and pmCherry Paxillin (#50526 ...
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bioRxiv - Cancer Biology 2023Quote: ... were amplified by PCR and cloned into pLenti-GFP-puro (Addgene plasmids #17481) Lentivirus were produced by transforming pLenti constructs ...
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bioRxiv - Developmental Biology 2023Quote: ... Double stranded RNA (dsRNA) was synthesized using pJC53.2 plasmids (Addgene Plasmid ID: 26536), as previously described 80 ...
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bioRxiv - Developmental Biology 2023Quote: Riboprobes for in situ hybridization were synthesized using the oligonucleotide primers listed in Supplementary Table 4 to clone the DNA fragment of interest into vector pJC53.2 (Addgene Plasmid ID: 26536), followed by riboprobe synthesis previously described 80.
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bioRxiv - Cancer Biology 2023Quote: ... a gift from Feng Zhang (Addgene #62988). The resulting plasmid was introduced into RPE1 by transfection with Lipofectamine 3000 (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: GFP protein fused with a nuclear localization signal (GFP-NLS) was stably transduced into HDFs using lentiviruses (pTRIP-SFFV-EGFP-NLS; Addgene, Cat# 86677). Cells were cultured on eight-well coverslip-bottomed slides (ibidi GmbH ...
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bioRxiv - Cancer Biology 2023Quote: pBMN-FKBP-YFP destabilization domain plasmid was purchased from Addgene RRID:Addgene_31763 ...
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bioRxiv - Cancer Biology 2023Quote: ... was bought from Addgene (Watertown, MA, USA, Addgene ID: 73179). Plasmid library were amplified by electro-transformation (Lucigen ...
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bioRxiv - Cancer Biology 2023Quote: ... was purchased from Addgene (plasmid # 109334, Watertown, MA). pInducer20 puro was a gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... pInducer20 blast (Addgene) was purchased from Addgene (plasmid # 109334 ...
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bioRxiv - Cancer Biology 2023Quote: Cas9: plenti-EF1a-Cas9-2A-blast and pXPR047 (Cas9-GFP reporter, Addgene# 107145) plasmids were provided by Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... or pLenti-PGK-tdTomato-Akaluc (TdT-AkaLuc) virus (subcloned from pLenti-PGK-Venus-Akaluc(neo)) and HR180-LGR5-iCT plasmids through Gibson assembly (Addgene 124701; 129094). For cecal injections ...
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bioRxiv - Cancer Biology 2023Quote: ... and pVSVg (Addgene, 14888), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... The oligonucleotides corresponding to the gRNA sequences were cloned in the pLentiCRISPRv1 vector (Addgene Plasmid # 49535), a doxycycline-inducible lentiviral vector ...
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bioRxiv - Cancer Biology 2023Quote: ... cloned in a doxycycline-inducible lentiviral vector Tet-pLKO-puro vector (Addgene #21915). Daoy medulloblastoma cells were transduced with the lentiviral particles carrying the shRNA construct and stably transduced cells were selected in the presence of puromycin ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting XRN1 sg1 and sg2-resistant XRN1 constructs and a GFP control construct were sub-cloned into the pLEX307 lentiviral expression vector (Addgene) under the control of an EF-1α promoter ...
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bioRxiv - Cancer Biology 2023Quote: ... Individual gRNAs (Table S2) against murine and human SMARCA4 were cloned into pLentivectorCRISPR v2 (52961, Addgene). Non-target control (NTC ...
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bioRxiv - Biochemistry 2023Quote: A pET29a-YS14 plasmid containing Xenopus laevis histones was obtained as a gift from Jung-Hyun Min (Addgene plasmid # 66890). H2A-H2B was subcloned into pET His6 TEV LIC cloning vector (2B-T ...
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bioRxiv - Biochemistry 2023Quote: ... the tagging plasmid pTF277 (Addgene 44094) was altered by PCR to replace the 2xStrep tag with 3xMyc ...
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bioRxiv - Cancer Biology 2023Quote: ... The pLKO.1 (Addgene #8453) lentiviral vector was digested with AgeI (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... gRNAs targeting TP63 were cloned into lentiCRISPR v2 (a gift from Feng Zhang, Addgene plasmid # 52961). An empty LCV2-Cas9 construct was used as a control ...
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bioRxiv - Cell Biology 2023Quote: ... pRK5-HA-Ubiquitin-WT was a gift from Ted Dawson (Addgene plasmid 17608; http://n2t.net/addgene:17608; RRID:Addgene_17608) (Lim et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The scrambled control construct was acquired from Addgene (Plasmid no #26701).
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bioRxiv - Cell Biology 2023Quote: pTriEx-NTOM20-LOV2 (Addgene plasmid # 81009) and pTriEx-mCherry-Zdk1 (Addgene plasmid # 81057 ...
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bioRxiv - Immunology 2023Quote: ... The lentiCRISPRv2-puro vector containing the Cas9 expression cassette and the gRNA scaffold was a gift from Brett Stringer (Addgene plasmid #98290 ...
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bioRxiv - Immunology 2023Quote: ... MIGR1 was a gift from Warren Pear (Addgene plasmid #27490). The mutations in ST10A were alanine substitutions for S336 ...
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bioRxiv - Immunology 2023Quote: ... pMD2.G and pCMVR8.74 were kindly provided by Didier Trono (Addgene plasmid #12259 & #22036).
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bioRxiv - Immunology 2023Quote: Full-length mouse Stat1 (NM_001205313.1) and Irf1 (NM_008390.2) coding sequence was cloned together with V5-TurboID adapted from Addgene plasmid V5-TurboID-NES_pCDNA3 (Addgene # 107169 ...
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bioRxiv - Immunology 2023Quote: ... Oligos were ligated into LentiCRISPRv2 vector (Addgene catalog # 52961 ...
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bioRxiv - Immunology 2023Quote: ... coding sequence was cloned together with V5-TurboID adapted from Addgene plasmid V5-TurboID-NES_pCDNA3 (Addgene # 107169) into the pCW57.1 mCherry vector (Catalog # 41393 ...
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bioRxiv - Immunology 2023Quote: ... Sortase A pentamutant (eSrtA) in pET29 was a gift from David Liu (Addgene plasmid # 75144) and was expressed as published (64) ...
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bioRxiv - Genomics 2023Quote: ... FKBP12F36V-mNeonGreen-V5 (for C-terminal tagging) was amplified from pAAV-hSOX9-dTAG-mNeonGreen-V5 (Addgene plasmid #194971).
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bioRxiv - Immunology 2023Quote: ... and psPAX2 plasmid (Addgene #12260) necessary for lentivirus production were obtained from Addgene ...
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bioRxiv - Immunology 2023Quote: ... The envelope pCMV-VSV-G plasmid (Addgene #8454) and psPAX2 plasmid (Addgene #12260 ...
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bioRxiv - Neuroscience 2023Quote: Birds were injected with the AAV9-CAG-GCaMP6f-WPRE-SV40 virus (100836-AAV9, Addgene). The total amount of virus injected was 897 nL (65 injections of 13.8 nL each) ...
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bioRxiv - Neuroscience 2023Quote: ... available from Addgene (Plasmid# 128618) followed by heat inactivation of the enzymes by incubation at 80°C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: The adeno associated viral vector AAV5-synapsin-DIO-GCAMP6s used in this study was purchased from Addgene. The AAV9-CMV-DIO-FLAG-MC3R ...
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bioRxiv - Neuroscience 2023Quote: ... The hM4Di virus was derived from Addgene plasmid 83896 ...
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bioRxiv - Neuroscience 2023Quote: ... mice received injections of either hM4Di virus or a control virus (pAAV-S5E2-GFP; Addgene #135631-AAV1). The hM4Di virus was derived from Addgene plasmid 83896 ...