-
No products found
because this supplier's products are not listed.
Logan Richards, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 2X Laemmli sample buffer (BioRad) was added to lysates and samples were incubated at 95°C for 5 mins ...
-
No products found
because this supplier's products are not listed.
Oana Păun, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... and resuspended in 2X Laemmli sample buffer (Sigma). Samples were then incubated at 95°C for 5 minutes and stored at -80°C until western blot analysis.
-
No products found
because this supplier's products are not listed.
M Wagstaff, et al.,
bioRxiv - Cell Biology 2024
Quote:
... each sample was diluted in 2x Laemmli buffer (ThermoFisher Scientific) plus Co-IP lysis buffer and heated to 95°C for 5 minutes prior to immunoblotting.
-
No products found
because this supplier's products are not listed.
Stamatis Papathanasiou, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Laemmli-SDS sample buffer (Boston BioProducts) was added ...
-
No products found
because this supplier's products are not listed.
Christopher Carlein, et al.,
bioRxiv - Physiology 2024
Quote:
... the samples were mixed with 2x Laemmli buffer (ref: S3401, Merck) in a 1:1 ratio and denatured for 5 min at 95 °C ...
-
No products found
because this supplier's products are not listed.
Marco Pirazzini, et al.,
bioRxiv - Pathology 2021
Quote:
... CGNs were lysed with Laemmli sample buffer containing protease inhibitors (Roche) or fixed for 10 min with 4% (w/v ...
-
No products found
because this supplier's products are not listed.
Brett A. Duguay, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... 4 µg of each lysate in 2X Laemmli buffer containing DTT/BPB was combined with GlycoBuffer 2 (1X final; NEB, P0704S) and NP-40 (1% final ...
-
No products found
because this supplier's products are not listed.
Elli M. Buchert, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... combine the following per sample: 25 μl 2x TDE1 Buffer (Illumina Cat #20034197), 3.5 μl Tn5 Enzyme (100nM final ...
-
No products found
because this supplier's products are not listed.
Fanny E. Hartmann, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
PCR Kit (Buffer 2X, Qiagen, USA), and 1.4 µL of the primer mix ...
-
No products found
because this supplier's products are not listed.
Marta Rigoli, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... An equal volume of 2x PLB buffer (Promega) was added to permeabilize the yeast cells ...
-
No products found
because this supplier's products are not listed.
Jung-Ho Chun, et al.,
bioRxiv - Biochemistry 2024
Quote:
... washed 2x with 1x Perm/Wash Buffer (BD), and incubated with anti-IFN-𝛾 (BioLegend ...
-
No products found
because this supplier's products are not listed.
Helena Hemberger, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 2X RNA Binding Buffer (Zymo Research) was added and vortexed for 10 seconds ...
-
No products found
because this supplier's products are not listed.
Melanie Werner-Klein, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 55 μl of 2x HiRPM hybridization buffer (Agilent, Germany), 11 μl of 10X GE Blocking agent (Agilent ...
-
No products found
because this supplier's products are not listed.
Patricia Ho, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Cells were washed 2X with FACS buffer and then fixed with Fixation Buffer (Biolegend), washed 2X with FACs buffer ...
-
No products found
because this supplier's products are not listed.
Andreja Zubković, et al.,
bioRxiv - Microbiology 2023
Quote:
... mixed with 2x Laemmli buffer with β- mercaptoethanol (Santa Cruz Biotech, Dallas, TX, USA), and denatured for 6 min at 95°C ...
-
No products found
because this supplier's products are not listed.
Daniel P. Ball, et al.,
bioRxiv - Immunology 2021
Quote:
... and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR), 100 mM DTT ...
-
No products found
because this supplier's products are not listed.
Marta E. Stremska, et al.,
bioRxiv - Immunology 2023
Quote:
... Supernatants were mixed with 5X Laemmli Buffer (0.3M Tris-H-Cl ...
-
No products found
because this supplier's products are not listed.
Yosuke Tanaka, et al.,
bioRxiv - Cell Biology 2024
Quote:
... the beads were boiled with 2 × Laemmli’s sample buffer and subjected to immunoblotting using an Amersham ECL Prime Western Blotting Detection System (GE Healthcare).
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No products found
because this supplier's products are not listed.
Ben Verpoort, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Proteins were eluted by heating Protein-G agarose beads and anti-HA magnetic beads for 15 min at 42°C in 50μL 2X sample buffer and analyzed by western blotting using rabbit anti-HA (1:1000, Cell Signaling Technology) and mouse anti-FLAG (1:1000 ...
-
No products found
because this supplier's products are not listed.
Roger Huerlimann, et al.,
bioRxiv - Microbiology 2024
Quote:
... 10 µl of 2x MightyAmp Buffer (Takara Bio Inc., Shiga, Japan), and 7.8 µl of nuclease-free water ...
-
No products found
because this supplier's products are not listed.
Maxim I. Maron, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... This was followed by addition of Laemmli buffer to 1x and blotting with H3 (Abcam, ab1791), CHTOP ...
-
No products found
because this supplier's products are not listed.
Max J. Kellner, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... gargle solution or sputum sample were mixed 1:1 with 2x QuickExtract DNA extraction solution (Lucigen) and heat inactivated for 5 minutes at 95°C ...
-
No products found
because this supplier's products are not listed.
Shogo Tsuji, et al.,
bioRxiv - Physiology 2022
Quote:
... the compact region of DMH from four DMH-Prdm13-KO or control mice was collected by laser microdissection into Laemmli’s sample buffer using the Leica LMD 6000 system (Leica) and boiled 5 min ...
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No products found
because this supplier's products are not listed.
Andrew Dimond, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... 25 μl 2X Tagmentation buffer (Diagenode, C01019043), 22.5 μl H2O ...
-
No products found
because this supplier's products are not listed.
Ciceron Ayala-Orozco, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 2X (10 mL) MEM vitamin solution (Corning, 25-020-Cl), 1X (5 mL ...
-
No products found
because this supplier's products are not listed.
Lisa L. Kirkemo, et al.,
bioRxiv - Biochemistry 2021
Quote:
Frozen cell and exosome pellets were lysed using 2X RIPA buffer (VWR) with protease inhibitor cocktail (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Meilin Zhu, et al.,
bioRxiv - Microbiology 2023
Quote:
... Fixative solution at 2X the working concentration contained 5% Glutaraldehyde (Electron Microscopy Services (EMS) #16000) ...
-
No products found
because this supplier's products are not listed.
Jean Farup, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The cells were then resuspended in wash buffer and incubated in MACS human FcR blocking solution (20μl/sample, cat nb. 130-059-901, Miltenyi Biotec) and primary antibodies against CD45-FITC (12μl/sample ...
-
No products found
because this supplier's products are not listed.
Karen Grimes, et al.,
bioRxiv - Genomics 2023
Quote:
... into 96-well plates containing 5 μl modified freeze buffer (0.1% NP-40, 7.5. % DMSO, 42.5 % 2X Profreeze-CDM (Lonza) in PBS ...
-
No products found
because this supplier's products are not listed.
Paul Knabl, David Mörsdorf, Grigory Genikhovich,
bioRxiv - Developmental Biology 2024
Quote:
... samples were incubated in Bloxall™Blocking Solution (Vector Laboratories) before the antibody blocking step (see Immunohistochemistry ...
-
No products found
because this supplier's products are not listed.
Iago Grobas, Marco Polin, Munehiro Asally,
bioRxiv - Biophysics 2020
Quote:
... 2x (Nikon Plan 2x UW, NA 0.06), 2.5x (Leica 2.5x N PLAN ...
-
No products found
because this supplier's products are not listed.
Alyssa Q. Stiving, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and 1X Sample Buffer (Perkin Elmer) in formamide (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Noam Cohen, et al.,
bioRxiv - Cell Biology 2021
Quote:
LCM samples were washed from lysis buffer by mixing with an equal portion of SPRI bead solution (Beckman Coulter, A63881), incubated 5 min R/T and washed twice with 100% EtOH under magnetic field ...
-
No products found
because this supplier's products are not listed.
Renata Varnaitė, et al.,
bioRxiv - Immunology 2020
Quote:
... samples were incubated in a 2% formaldehyde solution (Polysciences) for 2 h ...
-
No products found
because this supplier's products are not listed.
Ivana Cvijovíc, Michael Swift, Stephen R. Quake,
bioRxiv - Immunology 2023
Quote:
... The tissue solution was diluted in RoboSep buffer (Stemcell Technologies) and then passed through a 100µm cell strainer into a 50mL tube ...
-
No products found
because this supplier's products are not listed.
Ling-Zhen Liu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The perfusion solution Ringer’s buffer and perfusion solution II containing 1 mg/mL collagenase (Solarbio, Cat C8140) were used for toad liver tissue perfusion ...
-
No products found
because this supplier's products are not listed.
Oleh Krupa, et al.,
bioRxiv - Neuroscience 2021
Quote:
Imaging of cleared brain samples was performed using the Ultramicroscope II (LaVision Biotec) equipped with MVPLAPO 2X/0.5 NA objective (Olympus), sCMOS camera (Andor) ...
-
0.15 M MOPS, pH 7.5, 0.22 u filtered. Buffer concentrate used to buffer the constituted...
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Jin Li, et al.,
bioRxiv - Genomics 2024
Quote:
... followed by the replacement of buffer with 2ml ovomucoid solution (15 mg ovomucoid, Worthington Biochemical ...
-
No products found
because this supplier's products are not listed.
Meghan Barrett, et al.,
bioRxiv - Neuroscience 2022
Quote:
Brains were dissected in Phosphate Buffer Solution (PBS; MP Biomedicals LLC). Adult optic lobes (OL ...
-
No products found
because this supplier's products are not listed.
Laila A. Damiati, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Following two washes, the samples were incubated in 200 μl of substrate solution (tetramethylbenzidine, TMB solution) (R&D systems) per sample for 20 min ...
-
No products found
because this supplier's products are not listed.
Li Huang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Sample solutions were filtered using a 100 kDa microcentrifuge membrane filter (Sartorius) (3 × 5 minutes ...
-
No products found
because this supplier's products are not listed.
Jan Bohl, et al.,
bioRxiv - Biochemistry 2023
Quote:
... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
-
No products found
because this supplier's products are not listed.
Eloise J. Lockyer, et al.,
bioRxiv - Microbiology 2022
Quote:
... Samples were washed 2x in dH2O and were then transferred to a Pelco BioWave Pro+ microwave (Ted Pella Inc, Redding, USA) for a further two dH2O washes in the Biowave without vacuum (at 250W for 40s) ...
-
No products found
because this supplier's products are not listed.
Aaron Held, et al.,
bioRxiv - Neuroscience 2022
Quote:
... They were then washed 2x in 1xPBS and incubated for 1 hour in a secondary solution of PBT with donkey anti-chicken 647 (Jackson Immunoresearch 703-605-155) and Hoechst (1:1000 ...
-
No products found
because this supplier's products are not listed.
Han Wang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 2–3 μL of sample solution was applied to a holey carbon grid (Quantifoil R2/1 ...
-
No products found
because this supplier's products are not listed.
Lisa Leib, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Samples were resuspended in two volumes of buffer NTB (Macherey-Nagel; #740595.150) and DNA was purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel ...
-
No products found
because this supplier's products are not listed.
Charles Bayly-Jones, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Enzyme solution in buffer was applied and subsequently substituted with inhibitor/DMSO normalisation (Digital Dispenser D330e, Tecan, Switzerland) and preincubated at 30 °C for 20 min ...
-
No products found
because this supplier's products are not listed.
Yasmin Bay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Drug compound solutions were prepared in assay buffer in clear V-bottom 96-well plates (GR-651161, Greiner). Typically ...
-
No products found
because this supplier's products are not listed.
S.A. Kitchen, et al.,
bioRxiv - Genetics 2020
Quote:
... an individual larva was incubated in 12 µl of lysis solution (10.8 µl Buffer TL (Omega BioTek, Norcross, GA), 1 µl of OB Protease Solution (Omega BioTek ...
-
No products found
because this supplier's products are not listed.
Song Xue, et al.,
bioRxiv - Biochemistry 2021
Quote:
Samples of bFGF phase separation in solution or on cell surface were examined on an inverted microscope (LSM 780, Carl Zeiss, Germany) equipped with a confocal spinning disk unit (CSU-X1 ...